Objective: To explore the possible role of Notch signaling pathway in regulating nerve regeneration under hypoxic preconditioning conditions by using a mouse hypoxic preconditioning model. Methods: The hypoxic preconditioning model was established by intracerebroventricular injection of γ-secretase inhibitor DAPT in ICR mice. According to the number of hypoxic exposures, the mice were randomly divided into normoxic group (C group), hypoxic group (H group), hypoxic preconditioning group (HPC group), normoxic+dimethyl sulfoxide control group (DMSO group), normoxic+DAPT group (C+DAPT group), hypoxic+DAPT group (H+DAPT group) and hypoxic preconditioning+DAPT group (HPC+DAPT group). The expression of Notch1 and its downstream effector Hes1 mRNA and protein in hippocampus were detected by Western-blot and RT-PCR. The cell proliferation of hippocampal dentate gyrus was detected by immunofluorescence. Results: Compared with group C and group H, after 7 days of hypoxic preconditioning, the mRNA and protein levels of Notch1 increased (P＜0.05), and the protein expression of Hes1 increased (P＜0.05). Compared with H group, the number of Brdu positive cells in HPC group was decreased (P＜0.05). Compared with DMSO group, the mRNA and protein levels of Notch1 in each DAPT injection group were decreased (P＜0.05), and the increase of Hes1 protein expression was reversed. Conclusion: Hypoxic preconditioning may activate the Notch signaling pathway and regulate neuronal regeneration in the hippocampus of mice.
YAN Chang
,
XU Wenqiang
,
XIE Yabin
,
XIE Wei
. Mechanism of hypoxic preconditioning induced hippocampal neuron regeneration in mice through Notch signaling pathway[J]. Journal of Baotou Medical College, 2025
, 41(7)
: 53
-58
.
DOI: 10.16833/j.cnki.jbmc.2025.07.010
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