Objective: To explore the correlation between anxiety, depression symptoms and gastrointestinal symptoms in patients with gastrointestinal diseases in rural areas of Fuyang City and Bozhou City in Anhui Province. Methods: Using stratified random sampling method, 40 township hospitals were selected from Fuyang and Bozhou cities in Anhui province. A face-to-face survey was conducted among 309 gastrointestinal patients who met the inclusion criteria. Spearman rank correlation test and negative binomial regression model were used to analyze the association between patients’ anxiety, depression symptoms and gastrointestinal symptoms. Results: Among patients with at least one gastrointestinal symptom, the detection rates of anxiety symptoms and depression symptoms were 30.3% and 46.9%, respectively. Spearman rank correlation analysis showed that the duration of swallowing discomfort and loose stool were positively correlated with anxiety symptom score (r_s=0.441, 0.263, P＜0.05); the duration of hiccups or belching, postprandial mood swings, and the frequency of abdominal pain per year were positively correlated with depression symptom score (r_s=0.289, 0.306, 0.273, P＜0.05). The negative binomial regression analysis results showed that age, history of hospitalization in the past three months, history of gastrointestinal drug use in the past month, anxiety symptom scor, and depression symptom scor were influencing factors of the number of gastrointestinal symptoms (P<0.05). Conclusion: Patients presenting with at least one gastrointestinal symptom exhibit a higher detection rate of anxiety and depressive symptoms. Furthermore, the severity of anxiety and depression correlates with the number of gastrointestinal symptoms reported. Primary care doctors should pay attention to the screening and intervention of anxiety, depression and other psychological disorders in patients with gastrointestinal diseases.

Objective: To explore the regulatory effect of ursolic acid on the expression of inflammatory factors associated with cervical cancer cells (HeLa) by inhibiting the synthesis of prostaglandin E2 (PGE₂). Methods: HeLa cells were selected as the research subjects, and the logarithmic growth phase cells were divided into blank control group, ursolic acid group, cyclophosphamide group, ursolic acid+cyclophosphamide group, and ursolic acid+EP₂ receptor agonist group. The mRNA and protein expressions of interleukin-6 (IL-6), IL-1β and tumor necrosis factor α (TNF-α) in HeLa cells were detected by RT-PCR and Elisa. The Toll-like receptors (TLR4)/ myeloid differentiation factor 88 (MyD88)/NF-κB signaling pathway in HeLa cells was detected by Western blot, and the signaling pathway agonist was used to verify the pathway. Results: Compared with the blank control group, the expression of IL-6, IL-1β and TNF-α mRNA and protein in ursolic acid group was significantly decreased (P＜0.05). After adding PGE2 agonist, the expression of IL-6, IL-1β and TNF-α mRNA and protein was significantly higher than that in ursolic acid group (P＜0.05). Compared with the blank control group, the expression of TLR4, MyD88 and NF-κB protein in ursolic acid group was significantly decreased (P＜0.001). After adding PGE2 agonist, the expression of TLR4, MyD88 and NF-κB protein was significantly higher than that in ursolic acid group (P＜0.001). Compared with the ursolic acid group, TLR4, MyD88, and NF-κB signaling pathways were significantly activated after the addition of TLR4 agonist (LPS) (P＜0.001). Conclusion: Ursolic acid can inhibit the expression of PGE2 by inhibiting the TLR4/MyD88/NF-κB signaling pathway, thereby regulating the expression of inflammatory factors related to the development of HeLa cells.

Objective: This study employs the theoretical framework of the health ecological model to examine the current status and influencing factors of fertility intentions among the reproductive-aged population. It does so by considering perspectives at individual, family, community, and macro policy levels. The findings aim to provide a foundation for developing a comprehensive fertility support system and enhancing fertility intentions within this demographic group. Methods: A total of 1 851 questionnaires were collected nationwide through voluntary sampling, which is a non-probability sampling method. Binary logistic regression analysis was employed to examine the factors influencing the willingness to have a second or third child. Results: The factors influencing the willingness to have a second child differ between men and women. For men, those who identify with the belief that children are the most important people in the family tend to be more inclined towards having a second child. In contrast, for women, higher personal monthly income, having more siblings, recognizing maternal achievement, and access to quality community childcare services are associated with a greater willingness to have a second child. Conversely, women from families with lower economic status exhibit less inclination towards having a second child. Among female respondents, compared to those in Northeast China, individuals from Central China demonstrate lower willingness for third childbirths. Additionally, older respondents who experienced emotional abuse during childhood and those who recognize maternal achievement as significant show higher willingness for third childbirths when they also express satisfaction with reproductive policies. Conclusion: The improvement of fertility intentions among people of childbearing age depends on comprehensive interventions from multiple aspects such as individuals, families, and communities. It is necessary to establish a comprehensive fertility support system to provide assistance in boosting the fertility intentions of couples of childbearing age.

Objective: To analyze the nutritional status and influencing factors of elderly people living at home using the mini-nutritional assessment (MNA) method. Methods: A total of 380 elderly people (aged ≥58 years) living at home from October 2022 to January 2024 were selected as the research subjects. Their demographic data, life behavior information, and nutritional and health status were collected. A multiple linear regression model was used to analyze the factors influencing the nutritional status of elderly people living at home. Results: The research shows that the maximum age of the elderly living at home is 89 years old, with an average age of (70.23±5.86) years old. Among them, there are 189 males (49.74%) and 191 females (50.26%). The assessment score of the MNA scale for home-based elderly people was (24.94±3.24) points. Among them, 55 people had the risk of malnutrition, and 27 people were clearly diagnosed with malnutrition (accounting for a total of 21.58%). Through the analysis of the multiple linear regression model, it was found that the main risk factors affecting the nutritional status of the elderly living at home were age, marital status, satiety, and the number of chronic diseases (β′=-0.142, 0.112, 0.194, 0.124, P＜0.05). The protective factors influencing the nutritional status of elderly people living at home include high economic level, active activity ability in daily life, and the use of health supplements or nutritional supplements (β′=0.158, 0.105, 0.113). Conclusion: The micro-nutritional assessment method can be used to analyze the nutritional status and influencing factors of elderly people living at home, and has a relatively high assessment value.

Objective: To improve the quality standard of Mengyao Senden Siwei Soup. Methods: The microscopic identification of wenguan wood, chebula, gardenia and kawakuem seed in Senden four-flavor soup was established. Thin layer chromatography (TLC) was used to qualitatively identify wenguan wood, gardenia and chebula. The content of epicatechin in Junyao Wenguan wood was determined by high-performance liquid chromatography (HPLC) under the conditions of Supersil ODS-BP column (250 mm×4.6 mm, 5 μm), column temperature of 30 ℃, acetonitrile-1% formic acid solution (10∶90) as mobile phase, 280 nm as the detection wavelength, and the flow rate was 1.0 mL/min. Results: The microscopic characteristics of wenguan wood, gardenia, kawakuem seed and chebula were obvious. Thin-layer chromatography was used to analyze the results of wenguan wood, gardenia, kawakuem seed and chebula, and the results showed that the spots of each component were well separated and the negative control was not disturbed. The quantitative analysis of epicatein showed that the linear relationship was good in the concentration range of 0.018 7-0.138 8 mg/mL, and the regression equation could be expressed as Y=7E+06X-11 135 (correlation coefficient R²=0.999 3). The results of methodological validation showed that the average recovery rate of the sample recovery test was 98.17%, and the relative standard deviation was 1.73%. In addition, the RSDs of precision, repeatability and stability tests were controlled within the range of 0.11%, 1.75% and 1.55%, respectively, indicating that the method was reliable and stable. Conclusion: The method constructed in this study is accurate, reliable, sensitive and reproducible, and can be used for the quality control of Mongolian medicine Senden Siwei decoction.

Objective: Improve the quality control standards for the Mongolian medicine preparation Garixi pill. Methods: The experiment employed microscopic identification methods to qualitatively identify the five herbal ingredients-Piper longum, Cinnamomum cassia, Punica granatum, Cinnamomum cassia, and Rheum palmatum-in the Garixi pill formulation. Thin-layer chromatography (TLC) was selected to perform qualitative identification of Piper longum, Cinnamomum cassia, Cinnamon in the Garixi pill. Simultaneously, the content of piperine in the Garixi pill was determined using high-performance liquid chromatography (HPLC). An Elit SinoPak SP column (4.6 × 250 mm, 5 μm) was used, with methanol-water as the mobile phase for gradient elution. The column temperature was set to 30 ℃, the detection wavelength was 343 nm, and the flow rate was controlled at 1.0 mL/min. Results: The microscopic identification characteristics were obvious. The results of TLC identification showed that long pepper fruit, elecampane inula root and cassia bark had clear spots, strong specificity, good separation, negative without interference and easy identification. The peak area of piperine had a good linear relationship in the concentration range of 0.010 5-0.089 8 mg/mL. The regression equation was Y=1 110.7X+1.015 7 (R²=0.999 6). The average recovery was 105.25%, RSD=1.90%. Conclusion: The quality standard analysis method of Mongolian medicine Garixi pill established in this study has the characteristics of simple operation, accurate results, good repeatability and precision, and strong specificity, which can provide a reliable basis for the quality control of the preparation.

Objective: Through the integration of network pharmacology and molecular docking technology, the molecular regulation pathway and mechanism of action of Biflora mint drink in the treatment of diabetes-related periodontitis were elucidated, revealing its synergistic therapeutic properties of “multi-component-multi-target-multi-pathway”. Methods: Based on TCMSP database, 45 active core ingredients (e.g., quercetin, kaempferol) of the formula were screened, and the potential targets were predicted by Swiss Target Prediction. The targets related to diabetes mellitus combined with periodontitis were obtained from OMIM and DisGeNET databases, and 139 core targets were identified by the Venn diagram analysis. Cluster Profiler R software was used to analyze the GO/KEGG enrichment of the targets, and the “component-target-pathway” network was constructed, and the molecular docking affinity between the active ingredients and the key targets (e.g., TNF-α, IL-6) was verified by Auto DockVina. Results: The 14 core components of the Twoflower Mint Drink(e.g., quercetin, kaempferol) acted on 74 key targets and regulated 20 core pathways(including AGE-RAGE, IL-17, HIF-1 signaling pathway). The molecular docking showed that quercetin and other components had a binding free energy of -7.9 kcal/mol to TNF-α, suggesting a strong binding ability, and the GO analysis showed that the targets were enriched in inflammatory response, immune regulation and glucose metabolism. The KEGG pathway was involved in IL-17-mediated bone metabolism imbalance and AGE-RAGE inflammatory cascade. Conclusion: Double-flower mint drink can reduce inflammatory injury by synergistically inhibiting IL-6, TNF-α, IL-1β, regulating AGE-RAGE/NF-κB axis, intervening in IL-17 pathway to improve bone resorption, and regulating HIF-1-mediated adaptation to hypoxia, which embodies the multi-targeted and integrated mechanism of action of traditional Chinese medicine. This study provides a molecular basis for the treatment of diabetes mellitus combined with periodontitis by the compound formula and supports the optimization study of its quality standard.

Objective: To explore the effect of mevalonate diphosphate decarboxylase a (mvda) on the myocardial regeneration of zebrafish. Methods: On the 6th day after fertilization, the zebrafish embryos of the control group (DMSO), MTZ-treated myocardial injury group (MTZ), MTZ-treated group after mvda knock-out (mvda MO+MTZ) and MTZ-treated group after mvda overexpression (mvda OE+MTZ) were detected by confocal laser scanning microscopy. Results: Adding MTZ led to myocardial ablation of zebrafish. Compared with the MTZ group, mvda knocked-down could inhibit myocardial regeneration of zebrafish(P＜0.05), while regeneration ratio of ventricular in zebrafish with overexpression of mvda was higher(P＜0.05). It was found that zebrafish with overexpression of mvda had a higher rate of heart rate recovery to normal level than those with MTZ injury and mvda knocked down (P＜0.05). Zebrafish with overexpression of mvda could improve myocardial regeneration and promote recovery of cardiac function. Conclusion: Mvda can regulate the myocardial regeneration of zebrafish.

Objective: With the development of high-throughput sequencing technology, the demand for research at the single-cell level is constantly increasing. Especially for single-cell sequencing studies in skin tissues, the demand for dissociation into single-cell suspensions is growing day by day. However, the existing technology is difficult to dissociate skin tissue into single-cell suspensions in a short period of time. The dense structure of skin tissue leads to the fact that the existing dissociation methods are time-consuming and difficult to meet the timeliness requirements of downstream omics research. This experiment rapidly and effectively dissociates skin tissue through an innovative enzymatic digestion formula and mechanical processing method, meeting the requirements of single-cell research. Methods: Enzyme digestion method: Enzyme digestion method was divided into two groups, group A: type I collagenase+DNase I, group B: type II collagenase+0.25% trypsin+DNase I, both groups were prepared with Hanks buffer. The mechanical method was constant temperature shock and constant temperature beat. The enzyme digestion method and the mechanical treatment method were combined in pairs to treat the skin tissue. Results: The optimal dissociation method was enzyme digestion group A (type I collagenase 2 mg/mL, DNase I 10 U/mL)+constant temperature beating (180 times/min, 37 ℃). This combination achieved effective digestion of skin tissue within 15 min. The single cell suspension with high purity and high activity was successfully prepared by further treatment of hyaluronidase and red blood cell lysate, filtration (30 μm) and centrifugation. Conclusion: By optimizing the combination of enzyme types, concentrations and mechanical treatment methods, efficient digestion of skin tissue was achieved within 15 minutes, obtaining high-purity and high-activity single-cell suspensions. This greatly enhanced the efficiency and accuracy of the research, providing reliable technical support for subsequent flow cytometry analysis, cell sorting and high-throughput sequencing studies..

Objective: To explore the possible role of Notch signaling pathway in regulating nerve regeneration under hypoxic preconditioning conditions by using a mouse hypoxic preconditioning model. Methods: The hypoxic preconditioning model was established by intracerebroventricular injection of γ-secretase inhibitor DAPT in ICR mice. According to the number of hypoxic exposures, the mice were randomly divided into normoxic group (C group), hypoxic group (H group), hypoxic preconditioning group (HPC group), normoxic+dimethyl sulfoxide control group (DMSO group), normoxic+DAPT group (C+DAPT group), hypoxic+DAPT group (H+DAPT group) and hypoxic preconditioning+DAPT group (HPC+DAPT group). The expression of Notch1 and its downstream effector Hes1 mRNA and protein in hippocampus were detected by Western-blot and RT-PCR. The cell proliferation of hippocampal dentate gyrus was detected by immunofluorescence. Results: Compared with group C and group H, after 7 days of hypoxic preconditioning, the mRNA and protein levels of Notch1 increased (P＜0.05), and the protein expression of Hes1 increased (P＜0.05). Compared with H group, the number of Brdu positive cells in HPC group was decreased (P＜0.05). Compared with DMSO group, the mRNA and protein levels of Notch1 in each DAPT injection group were decreased (P＜0.05), and the increase of Hes1 protein expression was reversed. Conclusion: Hypoxic preconditioning may activate the Notch signaling pathway and regulate neuronal regeneration in the hippocampus of mice.

Objective: To analyze the association between the single nucleotide polymorphism (SNP) rs7217186 of the arachidonate 15-lipoxygenase (ALOX15) gene and the infection of Helicobacter pylori (H.pylori) as well as the risk of non-cardia gastric cancer. Methods: We performed a case-control study involving 458 patients with non-cardia gastric cancer and 460 healthy controls from the Han Chinese population in Baotou. H.pylori infection status in controls was assessed using both the ¹⁴C-urea breath test (UBT) and enzyme-linked immunosorbent assay (ELISA). Genotyping of ALOX15 rs7217186 was conducted via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The potential associations between this genetic variant and H.pylori infection or gastric cancer risk were evaluated using unconditional logistic regression analysis. Results: A total of 460 controls were tested for H.pylori infection, the positive rate was 42.3%.ALOX15 rs7217186 was not associated with infection by H.pylori. ALOX15 rs7217186 was not associated with the risk of non-cardia gastric cancer. Conclusion: ALOX15 rs7217186 polymorphism may not significantly contribute to H.pylori infection susceptibility or non-cardia gastric cancer risk.

Objective: To investigate the effect of sodium fluoride (NaF) on oxygen-glucose deprivation/rehydration (oxygen-glucose deprivation and reoxygenation,OGD/R) damage in microglia (BV2). Methods: OGD/R model of BV2 cells was constructed, treated with different concentrations of NaF, and used to determine the optimal timing by thiazole blue (methyl thiazolyl tetrazolium, MTT). BV2 cells were divided into control group (Control), OGD/R model group, 0.25 mmol/L NaF group, 2 mmol/L NaF group, 0.25 mmol/L NaF+OGD/R group, and 2 mmol/L NaF+OGD/R group. OGD/R molding was performed for 6 h after oxygen-glucose deprivation, except for control group and NaF groups. Cell viability was measured by MTT. Cell morphology was observed by an optical microscope. And lactate dehydrogenase (lactate dehydrogenase, LDH) content in the medium supernatant was detected by chromatometry. Results: After NaF exposure, the adhesion of BV2 cells decreased, and the number of cells decreased significantly, and the cells morphology changed. With the increase of NaF concentration and treatment time, cell viability decreased(P＜0.05). And the higher the NaF concentration, the lower the viability of BV2 cells. Compared with the control group, Cell viability was significantly decreased in the OGD/R group (P＜0.05). Compared with the OGD/R group, cell viability of BV2 cells were significantly decreased (P＜0.05), and the release of LDH increased significantly in 0.25 mmol/L NaF+OGD/R group and 2 mmol/L NaF+OGD/R group (P＜0.05). Compared with 0.25 mmol/L NaF group and 2 mmol/L NaF+OGD/R group, cell viability of BV2 cells decreased (P＜0.05) and the release of LDH increased (P＜0.05) in 0.25 mmol/L NaF+OGD/R group and 2 mmol/L NaF+OGD/R group, respectively. Conclusion: Sodium fluoride can aggravate microglia damage induced by oxygen-glucose deprivation/reoxygenation.

Objective: The endophytes with antibacterial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans are isolated and screened from Schisandra chinensis fruit, providing more screening sources for developing new clinical antibacterial drugs. Methods: The inhibitory activities of the endophytes against the three test pathogens were detected by the plate standoff method and Oxford cup method, and the data were statistically analyzed by SPSS Statistics 20.0 software, and the genera of the endophytes were further identified by morphological observation, physiological and biochemical characteristics, and molecular biology methods. Results: 25 endophytic strains were isolated, among which the fermentation broth of strain GF20 had strong antibacterial activity against the three tested pathogenic bacteria. After identification, GF20 was determined to be a bacterium of the genus Bacillus. Conclusion: Schisandra chinensis fruits are rich in endophytic bacteria, which have different antibacterial activities against common pathogenic bacteria and can potentially develop new antibacterial drugs.

Objective: Comprehensive analysis of ANAPC4 expression and its clinical relevance in hepatocellular carcinoma (HCC), with exploration of underlying mechanisms. Methods: The mRNA expression data sets of HCC in GEO, TCGA, SRA, GTEx, Oncomine and ArrayExpress databases were collected, and the mRNA expression level of ANAPC4 was analyzed. The protein expression intensity of ANAPC4 was observed by downloading ANAPC4 immunohistochemical staining pictures from the THPA database. The receiver operating characteristic curve(ROC), summary receiver operating characteristics, sensitivity, specificity, negative and positive likelihood ratios were used to evaluate the potential value of ANAPC4 in the differential diagnosis of HCC. Begg test was used to evaluate data publication bias. The expression level of ANAPC4 was further verified by single cell analysis, and the potential effect of ANAPC4 gene on the growth of HCC cell lines was analyzed by CRISPR knockout screening technology. Differentially related genes were used for GO and KEGG pathway enrichment analysis. Results: A total of 35 data sets (2 731 HCC tissues vs. 3 486 normal tissues) were included in the study. The pooled standardized mean difference (SMD) was 0.65(95%CI: 0.45-0.84), the area under the SROC curve was 0.79(95%CI: 0.75-0.83), the sensitivity was 0.62(95%CI: 0.50-0.72), and the specificity was 0.84(95%CI: 0.74-0.90). The positive likelihood ratio was 3.79(95%CI: 2.50-5.75), and the negative likelihood ratio was 0.46(95%CI: 0.36-0.58). Begg test suggested that there was no statistically significant publication bias. Single-cell sequencing data analysis revealed that ANAPC4 is significantly upregulated in HCC cells, which was further confirmed at the single-cell level. CRISPR-Cas9 knockout experiments demonstrated that ANAPC4 deletion markedly impaired the proliferative capacity of HCC cells. Notably, genes positively correlated with high ANAPC4 expression were significantly enriched in the cell cycle pathway. Conclusion: ANAPC4 is highly expressed in HCC and has a good ability to identify HCC, and may be involved in the occurrence and development of HCC through the cell cycle pathway.

Objective: To observe the correlation between vaginal flora and sex hormone levels in postmenopausal vaginitis. Methods: Totally 30 patients with postmenopausal bacterial vaginitis were selected as the experimental group, and 30 postmenopausal healthy subjects were selected as the control group. The vaginal pH value, flora density, diversity and sex hormone levels were compared between the two groups. Results: The pH value of postmenopausal patients with bacterial vaginitis was higher than that of the control group(P＜0.05), the bacterial density of the Gram-positive bacteria group was lower than that of the control group(P＜0.05), and the bacterial diversity was higher than that of the control group(P＜0.05), the bacterial density of gram-negative bacilli and positive cocci was higher than that of the control group(P＜0.05), the levels of luteinizing hormone(LH), follicle-stimulating hormone(FSH) and prolactin(PRL) were higher than those of the control group, and estradiol(E2), progesterone(PRGE) and testosterone(TESTO) were lower than those of the control group, the differences between the two groups were statistically significant(P＜0.05), that was the higher the postmenopausal E2, PRGE and TESTO, the lower the PRL, and the higher the concentration of Gram-positive bacteria. Conclusion: The flora density of Gram-positive bacteria in the vaginal microecology of postmenopausal women is correlated with sex hormones.

Objective: To investigate the predictive value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) quantitative perfusion parameters combined with serum miR-6861-5p level in the prognosis of patients with primary liver cancer. Methods: A total of 78 patients with primary liver cancer from January 2022 to January 2024 were retrospectively selected as the research objects. According to the pathological stage, the patients were divided into stage Ⅰ-Ⅱ group (46 patients) and stage Ⅲ group (32 patients). DCE-MRI quantitative perfusion parameters [transport constant (K^Trans), rate constant (K_ep), volume percentage of extracellular space outside blood vessels (V_e)] and serum miR-6861-5p levels were compared between the two groups and patients with different prognosis. The correlation between each index and pathological stage, prognosis of patients and the predictive value of combined detection for prognosis of patients were analyzed. Results: K^Trans, K_ep, V_e and miR-6861-5p in stage Ⅰ-Ⅱ group were lower than those in stage Ⅲ group (P＜0.05). Compared with patients with good prognosis, patients with poor prognosis had higher levels of K^Trans, K_ep, V_e and miR-6861-5p (P＜0.05). The levels of K^Trans, K_ep, V_e and miR-6861-5 p were positively correlated with pathological stage and prognosis of patients (P＜0.05). The area under the curve (AUC) of DCE-MRI quantitative perfusion parameters (K^Trans, K_ep, V_e) combined with miR-6861-5p in predicting the prognosis of patients was 0.929, the Youden index was 0.812, the sensitivity was 95.24%, and the specificity was 85.96% (P＜0.05). Conclusion: DCE-MRI quantitative perfusion parameters and serum miR-6861-5p levels are related to the pathological stage and prognosis of patients with primary liver cancer. Combined detection has certain predictive value for the prognosis of patients.

Objective: To explore and analyze the predictive ability of bedside ultrasound in evaluating cardiac diastolic function, diaphragmatic function and the combination of the two in the weaning results of patients undergoing mechanical ventilation in intensive care unit. Methods: A total of 103 patients with mechanical ventilation who were hospitalized in the intensive care unit of the First Affiliated Hospital of Baotou Medical College from December 2022 to September 2023 were selected. According to the results of spontaneous breathing trial (SBT) before weaning, they were divided into successful weaning group (65 patients) and weaning failure group (38 patients). The diaphragm function index and cardiac diastolic function index of the two groups were observed. The statistical significance of diaphragm function and cardiac diastolic function parameters was statistically analyzed, and the ROC curve was drawn. Results: The successful weaning group exhibited higher LVEF, DE, DTF, e', tricuspid E/A, and TAPSE values compared to the failed weaning group. The successful weaning group exhibited significantly lower values for respiratory rate, E/e', and RVEDA/LVEDA ratio than the failed weaning group (P＜0.05).There was no significant difference in mitral valve E/A value between the successful weaning group and the failed weaning group (P＞0.05). DE, E/e′and TAPSE were the three parameters with the largest AUC in the evaluation of diaphragmatic function and cardiac diastolic function, respectively, and the AUC of the combination of the three was 0.904. Conclusion: Bedside ultrasound assessment of cardiac diastolic function and diaphragmatic function in ICU patients with mechanical ventilation can accurately predict and analyze the weaning results of patients with mechanical ventilation, in order to reduce the occurrence of weaning failure events.