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  • CHU Ning, GAO Fang, JIA Yanbin
    Journal of Baotou Medical College. 2024, 40(6): 90-96. https://doi.org/10.16833/j.cnki.jbmc.2024.06.017
    铁死亡(Ferroptosis)是一种铁依赖性的新型的细胞程序性死亡(PCD)方式。花生四烯酸脂氧合酶-15(Arachidonate 15-lipoxygenase,ALOX15)是铁死亡的重要调控因子,它可以催化多不饱和脂肪酸(Polyunsaturated fatty acid,PUFA)生成脂质氢过氧化物,从而促进铁死亡的发生。ALOX15参与了多种癌症的发生发展,并发挥重要作用。本文对铁死亡的重要调控因子ALOX15与乳腺癌、食管癌、胃癌、结直肠癌以及前列腺癌的相关性进行综述,为相关肿瘤的研究提供参考。
  • WANG Haiyan, ZHANG Yonghong
    Journal of Baotou Medical College. 2024, 40(6): 25-29. https://doi.org/10.16833/j.cnki.jbmc.2024.06.005
    Objective: To investigate the influencing factors and roles of serum Cys C in microangiopathy of type 2 diabetes mellitus.Methods: A total of 830 patients with T2DM admitted from September 2020 to June 2022 were selected as the research objects, and the patients were divided into observation group (type 2 diabetic microangiopathy group) and control group (simple type 2 diabetes group) according to whether they had microangiopathy. The basic data and biochemical indexes of the two groups were compared. Results: The prevalence of hypertension, duration of diabetes and age in the observation group were higher than those in the control group (P<0.05). The levels of glycosylated hemoglobin (HbA1c), fasting blood glucose (FPG), serum creatinine (Scr), uric acid (UA), serum cystatin C (Cys C), low density lipoprotein (LDL), triglyceride (TG), serum total cholesterol (TC), apolipoprotein A (APOA) and apolipoprotein B (APOB) in the observation group were higher than those in the control group, and the level of high density lipoprotein (HDL) was lower than that in the control group (P<0.05). Binary logistic regression analysis showed that hypertension, course of disease, HbA1c, FPG, Cys C, HDL and APOA were the influencing factors of T2DM microangiopathy. The receiver operating curve (ROC) results showed that Cys C could be used as a potential diagnostic indicator for T2DM microvascular disease (AUC=0.707, P=0.000). Conclusion: HbA1c, FPG, HDL, APOA, hypertension, duration of diabetes, Cys C and other factors are independent influencing factors of T2DM microangiopathy, and serum Cys C can be used as a potential diagnostic indicator. The focus of T2DM microangiopathy management is not only to control blood glucose, but also to find prevention and treatment targets from a new perspective, providing new ideas for the prevention and treatment of T2DM microangiopathy.
  • ZHANG Shanshan, LI Yumei, HUANG Zhuhua, BI Minghong
    Journal of Baotou Medical College. 2024, 40(5): 33-38. https://doi.org/10.16833/j.cnki.jbmc.2024.05.007
    Objective: To compare the treatment and prognosis of kirsten rat sarcoma viral oncogene (KRAS) mutant lung adenocarcinoma patients with different mutation states and molecular typing. Methods: The data of patients with advanced KRAS mutant lung adenocarcinoma who were diagnosed and received first-line therapy in the First Affiliated Hospital of Bengbu Medical College from April 2019 to April 2022 were collected. The co-mutation of TP53, molecular typing and the correlation of treatment prognosis were analyzed. Results: Follow-up to April 2022, the mPFS of patients with KRAS/TP53 co-mutation was significantly shorter than that of patients with KRAS single mutation (HR=0.613, 95%CI: 0.396-0.951, P=0.029), and the difference was statistically significant. The mPFS of chemotherapy combined with anti-angiogenesis therapy was significantly prolonger than that of chemotherapy alone (HR=0.593, 95%CI: 0.355-0.990, P=0.038). The mPFS of chemotherapy combined with immunotherapy group was significantly longer than that of chemotherapy alone (HR=0.426, 95%CI: 0.247-0.736, P=0.02). There was no significant difference in mPFS between chemotherapy combined with anti-angiogenesis therapy group and chemotherapy combined with immunotherapy group (HR=0.648, 95%CI: 0.371-1.130, P=0.126). Conclusion: TP53 is a poor prognostic factor in patients with advanced KRAS mutant lung adenocarcinoma. There is no significant difference in treatment and prognosis between different molecular subtypes of G12C and non-G12C. Chemotherapy combined with immunotherapy or anti-angiogenesis therapy can prolong PFS in patients with advanced lung adenocarcinoma compared with chemotherapy alone, but there is no significant difference in PFS between the combined treatment groups.
  • CHENG Hong
    Journal of Baotou Medical College. 2024, 40(2): 78-82. https://doi.org/10.16833/j.cnki.jbmc.2024.02.014
    Objective: To study the causal relationship between vitamin D and hyperemesis gravidarum by two-sample Mendelian randomization. Methods: Based on the IEU OpenGWAS project website, the GWAS data set of vitamin D with the largest sample size and the GWAS data set of hyperemesis gravidarum were searched. The SNPs strongly associated with vitamin D were selected from the ieu-b-4808 library, and P<5×10-8 with a linkage disequilibrium coefficient of 0.001 and a region width of 10 000 kb were set. SNPs associated with hyperemesis gravidarum were selected from finn-b-O15_EXCESS_VOMIT_PREG. The combined data set included 174 SNPs as instrumental variables. The association between vitamin D and hyperemesis gravidarum was analyzed by four regression models: random effect inverse variance weighting (IVW), MR-Egger, weighted median, and weighted model. Results: The MR-Egger regression intercept was 0.005, and the corresponding P value was 0.513, suggesting that the SNPs had no gene pleiotropy. IVW regression results showed that OR(95%CIs) was 0.478(0.303-0.753), P value was 0.001; the results of MR-Egger, weighted median and weighted model were similar to those of IVW. The Cochran's Q values of IVW and MR-Egger regression were 143.733(P=0.893) and 143.303(P=0.888), respectively, suggesting that there was no heterogeneity between SNPs. Conclusion: Vitamin D is one of the protective factors of hyperemesis gravidarum in pregnancy.
  • WU Peng, YANG Zhanjun
    Journal of Baotou Medical College. 2024, 40(6): 1-6. https://doi.org/10.16833/j.cnki.jbmc.2024.06.001
    Objective: To investigate the protective effect of Dracocephalum moldavica L. on stroke based on network pharmacology. Methods: The effective components of Dracocephalum moldavica L. were searched by NCBI and CNKI. The effective targets of Dracocephalum moldavica L. were predicted by Pubchem and swiss target prediction databases. The target genes related to cerebral stroke were searched by GeneCards and Omim databases. The Venn diagram was constructed to screen the intersection genes. Cytoscape was used to establish and visualize the network diagram of drugs, components, targets and diseases. String software was used to construct the protein network interaction diagram. GO enrichment and KEGG analysis were performed using the DAVID database. Results: A total of 23 effective components of Dracocephalum moldavica L. were found by searching the literature, and there were 1682 corresponding targets. The main molecules were NADPH oxidase 4 (NOX4), xanthine dehydrogenase (XDH), myeloperoxidase (MPO), etc. Through PPI topology analysis, 57 core targets were obtained; GO enrichment analysis showed that 443 biological processes (BP), 104 molecular functions (MF) and 66 cellular components (CC) were involved. The results of KEGG showed that 143 signaling pathways were involved, including cancer pathway (hsa05200: pathways in cancer), PI3K-Akt signaling pathway (hsa04151: PI3K-Aktsignaling pathway), neuroactive ligand-receptor interaction (hsa05022: neuroactive ligand-receptor interaction) and so on. Conclusion: This study preliminarily revealed that Dracocephalum moldavica L. can treat stroke through a variety of targets and pathways, providing a theoretical basis for further scientific experiments.
  • ZHANG Xiaoxue, WANG Jinzhi, ZHAO Yue, QI Lina, WANG Ruiyue, HOU Bing, DOU Na
    Journal of Baotou Medical College. 2024, 40(5): 55-60. https://doi.org/10.16833/j.cnki.jbmc.2024.05.011
    Objective: To explore the correlation of upper limb motor function Fugl-Meyer rating scale (FMA-UE), simple upper limb function assessment scale (STEF) and test évaluant les membres supérieurs des personnes agées (TEMPA), to observe the ceiling effect or floor effect in patients with different degrees of stroke, and to compare the responsiveness of the three scales, so as to provide guidance for the selection of scales in clinic. Methods: FMA-UE, STEF and TEMPA were used to evaluate the upper limb function in patients with stroke. Spearman method was used to analyze the correlation of the three scales, and the proportion of people close to the maximum / minimum score was calculated to observe whether there was ceiling effect or floor effect. Twenty patients in subacute stage were selected for routine upper limb rehabilitation training. Two weeks later, the upper limb function was evaluated with TEMPA, FMA-UE and STEF, and the responsiveness of the three scales was observed. Results: The correlation coefficient between FMA-UE and STEF was 0.767, and that between STEF and TEMPA was 0.559~0.848, and that between STEF and TEMPA was 0.840~0.908. FMA-UE showed ceiling effect in patients with mild upper limb disorders, STEF showed significant floor effect in patients with moderate upper limb disorders, and all patients with severe upper limb disorders scored 0. TEMPA scale did not show ceiling effect or floor effect. The responsiveness of the three scales was good, including effect scale (ES): FMA-UE > STEF > TEMPA task analysis dimension > TEMPA function score dimension, standardized response mean (SRM), median effect scale (MES): FMA-UE > TEMPA task analysis dimension > STEF > TEMPA function score dimension. Conclusion: There is a high correlation and good responsiveness among the three upper limb function assessment scales. However, FMA-UE shows ceiling effect in patients with mild upper limb disorders. STEF is only suitable for the evaluation of patients with mild upper limb disorders. TEMPA scale does not find ceiling effect or floor effect, so it can be used widely.
  • WANG Bin, CAO Dengyi, WANG Yuanpeng, LI Naishu, CUI Wanjin
    Journal of Baotou Medical College. 2024, 40(2): 8-13. https://doi.org/10.16833/j.cnki.jbmc.2024.02.002
    Objective: To investigate the effect of Chemerin on the invasion and migration of colon cancer cells and its mechanism. Methods: Colon cancer cell lines HCT116 and SW480 were treated with different concentrations of Chemerin (0, 100, 200, 300, 400, 500 ng/mL), and the invasion and migration ability of cells were detected by Transwell method. HCT116 and SW480 cells were further studied and divided into 4 groups: pEGFP-N1 negative control group (vector control), si-Chemerin negative control group (si-NC), pEGFP-N1-Chemerin group and si-Chemerin group. After transfection, the invasion and migration ability of each group was detected. The mRNA levels of E-cadherin (E-cad), N-cadherin (N-cad) and vimentin were detected by qRT-PCR, and the protein levels of E-cad, N-cad and vimentin were detected by Western blot. Results: Exogenous Chemerin increased the invasion rate and migration number of colon cancer cells HCT116 and SW480 (P<0.05), which was dose-dependent, and cell migration number and invasion rate in pEGFP-N1-Chemerin group>vector control group, si-NC group>si-Chemerin group (P<0.05). The expression levels of E-cad mRNA and protein in pEGFP-N1-Chemerin group<vector control group, si-NC group<si-Chemerin group (P<0.05). The mRNA and protein expression levels of N-cad and vimentin in pEGFP-N1-Chemerin group>vector control group, si-NC group>si-Chemerin group (P<0.05). Conclusion: Chemerin may promote colon cancer cell invasion and migration by regulating epithelial-mesenchymal transition.
  • Yang Mei, WU Luofeng, LIU Shan
    Journal of Baotou Medical College. 2024, 40(6): 74-79. https://doi.org/10.16833/j.cnki.jbmc.2024.06.014
    Objective: To explore the mediating effect of attitudes toward aging on social isolation and self-perception aging of the elderly in rural communities. Methods: The convenience sampling method was used to investigate 422 elderly people in rural communities in Chengdu from June 2021 to May 2022 by using general data questionnaire, Aging Attitude Questionnaire(AAQ), Lubben Social Network Scale (LSNS-6) and the Brief Ageing Perceptions Questionnaire(B-APQ). Results: The B-APQ score of the elderly in the rural community was (51.80±5.21) scores, the LSNS-6 score was (16.59±3.05) scores, and the AAQ score was (78.24±6.03) scores. Self-perceived aging was positively correlated with social isolation (r=0.535, P<0.01) and negatively correlated with attitudes toward aging (r=-0.526, P<0.01); attitudes toward aging played a part of the mediating role between social isolation and self-perceived aging among the elderly in rural communities, and the amount of mediating effect was 0.39 [95%CI(0.245~0.432)], accounting for 52.0% of the total effect. Conclusion: Attitudes toward aging plays a part of the mediating role in the social isolation and self-perception aging relationship of the elderly in rural communities, and the community should help the elderly to establish a positive psychological state and reduce the negative impact caused by social isolation, so as to help the elderly in rural communities get more positive self-perception aging and promote healthy aging.
  • Gaohuricha, GUO Wuxia, CHEN Hong, LIU Mengyu, LI Xiaoxu, ZHAO Zhiying
    Journal of Baotou Medical College. 2024, 40(2): 1-7. https://doi.org/10.16833/j.cnki.jbmc.2024.02.001
    Objective: To explore the mechanism of Mongolian medicine Xinheisugawurile in the treatment of cerebral ischemia-reperfusion injury. Methods: The drug and disease targets were predicted by TCMSP, BATMAN and GeneCards databases, respectively. Venny was used to obtain their common targets, and STRING software was used to construct PPI network. The David database was used to carry out GO biological and KEGG pathway enrichment studies, and Cytoscape software was used to construct an active ingredient-key target-important pathway network. Finally, molecular docking was performed using Autodock and other software. Results: A total of 30 key targets, 15 important biological processes, 20 core KEGG pathways and 10 main active components were obtained for the treatment of cerebral ischemia-reperfusion injury by Mongolian medicine Xinheisugawurile. The results of molecular docking showed that the binding ability of the main active components to the key targets was ≤1.2 kcal/mol. Conclusion: Network pharmacology analysis shows that Mongolian medicine Xinheisugawurile mainly acts on estrogen receptor α (ESR1), nuclear factor-κBp65 (RELA), tumor protein p53 (TP53) and protein kinase B1 (AKT1) through β-carotene, baicalein, piperine, acacetin and other active ingredients. It regulates apoptosis, tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor-1 (HIF-1), phosphatidylinositol-3 kinase (PI3K)-AKT, estrogen and other signaling pathways, participates in the positive and negative regulation of apoptosis, inflammatory response, angiogenesis, hypoxia and other biological processes, so as to achieve the effect of treating cerebral ischemia-reperfusion injury.
  • QI Dong, YAO Chuanshun, YAO Muzi, JIA Yuan, HU Shumin
    Journal of Baotou Medical College. 2024, 40(2): 62-66. https://doi.org/10.16833/j.cnki.jbmc.2024.02.011
    Objective: To investigate the value of left atrial appendage CT angiography (CTA) double phase scan in the diagnosis of left atrial appendage thrombus in patients with atrial fibrillation. Methods: Thirty-six patients with atrial fibrillation were retrospectively collected from January 2020 to June 2022, and all patients underwent left atrial appendage (LAA) CTA double phase scan and transesophageal echocardiography (TEE). The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic compliance rate of LAA-CTA phase I and phase II diagnoses of LAA thrombus were calculated using the TEE test as the "gold standard", and the consistency of the LAA-CTA phase I and phase II diagnoses with the TTE diagnoses was evaluated using the Kappa test. Results: Twenty-one patients with left atrial appendage thrombus diagnosed in the first stage of LAA-CTA, 15 patients with left atrial appendage thrombus diagnosed in the second stage, and 14 patients with left atrial appendage thrombus diagnosed by TTE; the sensitivity, specificity, positive predictive value, negative predictive value and diagnostic compliance rate of LAA-CTA phase I for the diagnosis of LAA thrombus were 64.29%, 45.45%, 42.86%, 66.67% and 52.78%, respectively; the sensitivity, specificity, positive predictive value, negative predictive value and diagnostic compliance rate of LAA-CTA phase II for the diagnosis of LAA thrombus were 92.86%, 90.91%, 86.67%, 95.24% and 91.67%, respectively, with statistically significant differences (P<0.05). The results of LAA-CTA phase I diagnosis of left atrial appendage thrombus were in general agreement with TEE diagnosis (Kappa=0.089), while the results of LAA-CTA phase II diagnosis of left atrial appendage thrombus were in better agreement with TEE diagnosis (Kappa=0.827), with statistically significant differences (P<0.05). Conclusion: The sensitivity, specificity, and diagnostic compliance rate of the second-stage scan are higher, and the results of the second-stage diagnosis of thrombus are in better agreement with TEE, so the left atrial appendage CTA double phase scan method has higher clinical application value in the diagnosis of LAA thrombus.
  • WEI Jing, GUO Fangzheng, SONG Yamin, LI Baiqing, XU Tao, WANG Hongtao
    Journal of Baotou Medical College. 2024, 40(7): 23-31. https://doi.org/10.16833/j.cnki.jbmc.2024.07.004
    Objective:To study the prokaryotic expression and purification of GroEL1 from mycobacterium tuberculosis by genetic engineering and bioinformatics, and to predict its structure and function, and to analyze its application value in novel tuberculosis vaccine. Methods:The GroEL1 gene was amplified by PCR in vitro and cloned into the pET28a plasmid. The pET28a-GroEL1 vector was successfully constructed by sequencing and transformed into the expression strain of E. coli BL21 (DE3). The recombinant GroEL1 protein was expressed by IPTG and purified by nickel affinity column. The nucleotide and amino acid sequences of GroEL1 of H37Ra strain were obtained from UniProt database. Protparam, TMHMM-2.0, Protscale, NetChop-3.1, Psortb and SignalP-4.1 were used to predict the physicochemical properties, transmembrane helix, hydrophilic/hydrophobic, phosphorylation sites, subcellular localization and signal peptide of GroEL1 protein, respectively. NetNGlyc-1.0 and YinOYang-1.2 predicted the glycosylation sites. SOPMA and Swissmodel predicted the secondary structure and tertiary structure of the protein. Clustalw compares homologous sequences. String predicted interacting proteins, IEBD and ABCpred predicted B-cell epitopes of proteins. Results:The recombinant pET28a-GroEL1 vector was successfully constructed and the GroEL1 protein partially expressed in soluble form in E. coli. The recombinant GroEL1 protein was purified by nickel affinity chromatography with a purity of more than 90%. Western blot analysis confirmed that the recombinant GroEL1 protein had good immunoreactivity. The GroEL1 gene of mycobacterium tuberculosis strain H37Ra has a total length of 1 620 bp, encoding 539 amino acids with a molecular weight of 55.88 kD and an isoelectric point of 4.98. It is predicted that the protein has strong hydrophilicity, stable properties, no transmembrane region, 37 possible phosphorylation modification sites and 8 O-glycosylation sites, which is a non-secreted protein. Located in the cytoplasm, the secondary structure showed α-helix (53.43%), extended chain (11.87%), β-turn(7.61%), random coil(27.09%), multiple B cell antigen epitopes and multiple GroEL1 protein interacting proteins. Conclusion:The recombinant GroEL1 protein was successfully expressed and purified, and the structure and function of GroEL1 protein were predicted by bioinformatics, which laid a foundation for the prevention, diagnosis and treatment of tuberculosis.
  • ZHU Hongbo, DAI Yujing, ZHAO Jie, HUO Dongsheng
    Journal of Baotou Medical College. 2024, 40(6): 7-13. https://doi.org/10.16833/j.cnki.jbmc.2024.06.002
    Objective: To explore the mechanism of ginsenoside Rb1 in the treatment of Parkinson 's disease by using network pharmacology technology, and to provide scientific theoretical basis for the clinical rational application of ginsenoside Rb1. Methods: The targets of ginsenoside Rb1 were predicted in SwissTargetPrediction, SEA and SuperPred databases. The related targets of Parkinson's disease were obtained in the GeneCards and Omim databases using the keyword 'Parkinson Disease'. The ginsenoside Rb1 and the target of Parkinson's disease were mapped to each other, and the Veen diagram was made to obtain the intersection gene. Then, Cytoscape 3.8.2 software was used to construct the 'drug-target' network ; the 21 common drug targets related to Parkinson's disease in the Venn diagram were imported into the String (https://string-db.org/) database for protein-protein interaction prediction, so as to predict the core targets. The core targets of ginsenoside Rb1 in the treatment of Parkinson's disease were imported into the DAVID database to obtain GO analysis results and KEGG enrichment results. AutoDock Vina was used to perform molecular docking between ginsenoside Rb1 and key targets to verify their interaction activity. Results: There were four core targets of ginsenoside Rb1 in the treatment of Parkinson's disease, namely BCL2L1, VEGFA, FGF2, KDR, with 28 related pathways. Combined with the biological characteristics of ginsenoside Rb1, it was found that ginsenoside Rb1 treatment of Parkinson's disease mght be involved in the biological regulation process through the PI3K/Akt signaling pathway, and this regulation process was closely related to the mechanism of apoptosis in cell bioengineering. Through the molecular docking verification of the key target and ginsenoside Rb1, the target interaction was highly combined. Conclusion: The mechanism of ginsenoside Rb1 in the treatment of PD may play a role by reducing the biological process of apoptosis. The related pathways affected are PI3K/Akt signaling pathway, MAPK signaling pathway, Notch signaling pathway, and BCL2L1, VEGFA, FGF2, KDR and other targets.
  • ZHANG Zhaojun, SUN Jieke, LI Jianseng, SHI Yurong
    Journal of Baotou Medical College. 2024, 40(8): 1-6. https://doi.org/10.16833/j.cnki.jbmc.2024.08.001
    Objective: To explore the role of lipoyltransferase (LIPT1) in the development and immune infiltration of breast cancer by bioinformatics. Methods: TCGA, GEPIA, HPA, UALCAN, Kaplan-Meier, TIMER 2.0 and other databases were used to explore the differential expression, function and related mechanism of LIPT1 in breast cancer, and to analyze the effect of LIPT1 on the immune infiltration of invasive breast cancer. Results: The expression of LIPT1 was associated with invasive breast cancer, and PPI network analysis showed that LIPT1 was associated with ACSM1, DBT, DLAT, DLT, DLST, GCSH, LIAS, LIPT2, PDHB, and PDHX; the expression of LIPT1 was associated with immune cell infiltration in invasive breast cancer. Conclusion: Bioinformatics predicts that LIPT1 expression is down-regulated in invasive breast cancer and is associated with tumor immune cell infiltration.
  • LI Jishuai, ZHANG Rongxin
    Journal of Baotou Medical College. 2024, 40(6): 80-85. https://doi.org/10.16833/j.cnki.jbmc.2024.06.015
    Objective: To visually analyze the published literature of traditional Chinese medicine in the treatment of postpartum hypogalactia in CNKI using Citespace 6.1R6, and to summarize the current status, research hotspots and frontier development of traditional Chinese medicine in the treatment of postpartum hypogalactia. Methods: The literature related to the treatment of postpartum hypogalactia by traditional Chinese medicine collected by CNKI from January 1,2010 to December 21,2022 was searched. Citespace 6.1R6 was used to standardize the data based on bibliometrics, and the publication time, authors, institutions and keywords in the literature were visually analyzed and mapped. Results: A total of 464 articles were included, with a time span of 12 years, and the number of publications increased year by year. There were 359 authors in this field, of which 17 were high-frequency authors. The main research teams were ZHANG Shuying's team and LIU Manfang's team. A total of 525 research institutions were included, among which the headquarters and affiliated hospitals of Guangzhou University of Chinese Medicine, the headquarters and affiliated hospitals of Chengdu University of Traditional Chinese Medicine, and the headquarters and affiliated hospitals of Shandong University of Traditional Chinese Medicine were the top three institutions with the largest number of publications. A total of 327 keywords were included, forming 11 cluster labels. The top three keywords were 'lack of milk', 'acupoint massage', 'lactation volume'; there were 4 centrality >0.1. Conclusion: The research hotspots of traditional Chinese medicine in the treatment of postpartum hypogalactia are mostly the clinical efficacy observation literature of external treatment of traditional Chinese medicine, and the basic research is relatively few. It is necessary to strengthen the mining research of basic literature; some institutions have a deep understanding and good research results in this field, but the cooperation between teams and institutions is only internal communication, and there is less cross-regional cooperation. Cross-regional communication and cooperation should be strengthened to carry out deeper theoretical innovation.
  • ZHOU Chengli, GUO Jimin, LIU Yang, TANG Hongping
    Journal of Baotou Medical College. 2024, 40(2): 49-57. https://doi.org/10.16833/j.cnki.jbmc.2024.02.009
    Objective: To study the imaging findings of ultrasound (US), mammography (MG) and magnetic resonance imaging (MRI) in patients with pathological subtypes of breast papillary carcinoma, and to analyze the relationship between subtypes and imaging features of breast papillary carcinoma and common immunohistochemical indicators. Methods: A retrospective analysis of 47 cases of papillary breast cancer admitted from February 16, 2012 to December 20, 2021, including 47 cases of US examination (47 lesions), 45 cases of MG examination (45 lesions), and 14 cases of MRI examination (16 lesions). The relationship between subtype cases and their imaging features and immunohistochemical indicators such as ER, PR, Her-2 and Ki-67 was compared and analyzed. Results: US characteristics: In the expression of ER and PR, there was no significant difference in the ultrasound imaging characteristics of each subtype (P&gt; 0.05), the positive expression rates of ER and PR in 42 cases were 97.6% (41/42) and 88.1% (37/42), respectively. In the expression of Her-2, there was a statistically significant difference in the posterior echo of pDCIS subtype tumor (P=0.039). The tumor diameter of EPC subtype and the posterior echo of IPC subtype showed significant differences in Ki-67 expression (P=0.037,P=0.039). MG characteristics: There were statistical differences in the calcification distribution of SPC subtype in Ki-67 expression (P=0.048), and there was no statistically significant difference in the imaging characteristics of MG in ER, PR and Her-2 expression (P&gt; 0.05), the positive expression rates of ER and PR in 26 cases were 96.2% (25/26) and 80.8% (21/26), respectively. MRI features: There were no significant differences in MRI imaging features of ER, PR, Her-2 and Ki-67 expression subtypes (P&gt; 0.05), the positive expression rates of ER and PR in 13 cases were 100.0% (13/13) and 84.6% (11/13), respectively. The negative expression rate of Her-2 was 76.9% (10/13). Conclusions: Papillary breast carcinoma is a kind of breast cancer with positive expression of ER and PR. Tumor diameter ≥2 cm in EPC subtype and posterior echo attenuation of mass in IPC subtype on US are associated with positive expression of Ki-67. The positive expression of Ki-67 is related to the imaging characteristics of MG with non-benign distribution of calcification of SPC subtype. These imaging features are helpful to determine the biological characteristics of papillary breast carcinoma and to predict the prognosis of patients.
  • LI Chao, YU Dajun, BU Lyuting
    Journal of Baotou Medical College. 2024, 40(5): 65-71. https://doi.org/10.16833/j.cnki.jbmc.2024.05.013
    Objective: To explore the current status and relationship of electronic health literacy , health promotion behavior , and quality of life in elderly patients with chronic diseases. Methods: From August 1,2022 to February 20,2023, 325 chronic disease patients ≥60 years old were randomly selected using convenience sampling method. A structural equation model was constructed to analyze the relationship between electronic health literacy, health promotion behavior and health related quality of life in elderly patients with chronic diseases. Results: The total score of eHEALTH for patients was (18.73±10.64), the total score of health promotion behavior was (124.02±19.63), the total utility value of the European Five Dimensional Health Scale(EQ-5D-5L) was (0.79±0.22), and the EQ-VAS score was (72.35±16.21).The total score of patients' electronic health literacy is positively correlated with the total score of health promotion behavior (r=0.197, P<0.001); The total score of electronic health literacy is positively correlated with the score of quality of life (r=0.188, P<0.001), and the total score of health promotion behavior was also positively correlated with EQ-VAS scores (r=0.289, P<0.001). Structural equation model analysis showed that electronic health literacy in elderly patients with chronic diseases can directly affect their quality of life, with a direct effect of 0.168 (P<0.05); Electronic health literacy can also indirectly affect patients' quality of life by influencing their health promoting behavior, with an indirect effect of 0.046 (P<0.05). Conclusion: Medical institutions should attach importance to the level of electronic health literacy of patients, implement targeted health intervention measures, effectively improve the level of electronic health literacy, and thereby enhance their health promotion behavior and quality of life.
  • NIE Zeyin, LI Chenyu, CHEN Jiale, MIAO Huachun, WU Feng
    Journal of Baotou Medical College. 2024, 40(2): 14-19. https://doi.org/10.16833/j.cnki.jbmc.2024.02.003
    Objective: To observe the effect of electroacupuncture on the expression of high mobility group protein 1 (HMGB1) and phosphorylated c-Jun N-terminal kinase (p-JNK) in the anterior cingulate cortex of rats with cerebral ischemia, and to explore the protective effect and mechanism of electroacupuncture on the anterior cingulate cortex of rats with cerebral ischemia. Methods: Twenty-four male SD rats were randomly assigned into four groups, namely sham-operation, model, electroacupuncture, and sham-electroacupuncture groups, with 6 rats in each group. Cerebral ischemia was induced by blocking the right middle cerebral artery, creating a rat model of cerebral ischemia. Electroacupuncture was administered for 30 minutes, once daily for 14 days, targeting “Baihui”(GV20) and “Zusanli”(ST36) on the left side of the electroacupuncture group. In contrast, only the skin was penetrated in the sham-electroacupuncture group without activating the electroacupuncture device. Longa score was used to evaluate the neurological impairment of rats in each group. Nissl staining was used to observe the morphology and distribution of neurons in the right anterior cingulate cortex. The expression of HMGB1 and p-JNK protein in the right anterior cingulate cortex was detected by immunohistochemistry. Results: Compared with the sham-operation group, the neurological deficit scores of the model group and the sham-electroacupuncture group were increased (P<0.01), the number of Nissl positive neurons in the right anterior cingulate cortex was decreased (P<0.01), and the expression of HMGB1 and p-JNK protein was increased (P<0.01). Compared with the model group, the neurological deficit score of the rats in the electroacupuncture group decreased on the 7th and 14th day of cerebral ischemia (P<0.05), the number of Nissl positive neurons increased (P<0.01), and the expression of HMGB1 and p-JNK protein decreased (P<0.01). Conclusion: Electroacupuncture may reduce the damage of anterior cingulate cortex by inhibiting the overexpression of HMGB1 and p-JNK after cerebral ischemia.
  • FAN Zixu, LI Xingnan, JIN Min, WANG Zhanli, GAO Zhixiang
    Journal of Baotou Medical College. 2024, 40(5): 72-78. https://doi.org/10.16833/j.cnki.jbmc.2024.05.014
    Objective: To explore the mechanism of Mongolian medicine Sendeng-9 Decoction (Brucellosis Ⅱ) in the treatment of brucellosis by network pharmacology. Methods: The BATMAN-TCM database was used to analyze the active components and potential targets of Mongolian medicine Sendeng-9 decoction. Brucellosis-related targets were screened through Genecards, OMIM, and DisGeNET databases. The Venn diagram was constructed to obtain the intersection target of disease and the intersection target of Mongolian medicine Sendeng-9 decoction in the treatment of brucellosis. The network diagram of “drug-component-disease-target” relationship was drawn by Cytoscape software. The protein-protein interaction (PPI) network of ' active ingredient-disease ' common targets was constructed by STRING database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of key targets were performed using the David database. Results: There were 182 active components of Mongolian medicine Sendeng-9 Decoction, which acted on 1306 targets. There were 620 brucellosis targets and 152 drug-disease intersection targets. The main active ingredients of Mongolian medicine Sendeng-9 Decoction in the treatment of brucellosis were lauric acid, myristic acid, linoleic acid, jasmonone, lupinone, geranylacetone, oxysophocarpine, 3-nonen-2-one, etc. The core targets for the treatment of brucellosis were IL-6, IL-1β, INS, TNF, PTGS2, JUN, TP53, IGF1, CCL2, HIF1A. A total of 1064 GO entries were obtained, including 834 BP entries, 107 CC entries, and 123 MF entries. There were 162 KEGG signaling pathways, mainly focusing on HIF-1 signaling pathway, TNF signaling pathway, MAPK signaling pathway, FoxO signaling pathway, IL-17 signaling pathway and so on. Conclusion: Through the method of network pharmacology, this study preliminarily discusses the anti-inflammatory and disease-resistant pathogenic microorganisms of the effective components of Mongolian medicine Senden-9 decoction, which provides a theoretical basis for the treatment of brucellosis, and further study of the mechanism of action and clinical application of this prescription.
  • WANG Sibo, WU Tao, PANG Chunyan, WANG Hui
    Journal of Baotou Medical College. 2024, 40(2): 28-31. https://doi.org/10.16833/j.cnki.jbmc.2024.02.005
    Objective: To study the immunoregulatory effect of umbilical cord mesenchymal stem cells (UC-MSCs) on peripheral blood of patients with Sjogren's syndrome (SS). Methods: UC-MSCs were isolated and cultured from umbilical cord tissue of newborns, and the cell surface markers of UC-MSCs were detected by flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from 20 SS patients in the active phase of the disease. Co-cultures of PBMCs from SS patients with UC-MSCs were established, and cell viability was assessed using the CCK-8 assay. The optimal ratio of co-cultured cells was determined for subsequent experiments. Flow cytometry was used to analyze the changes in lymphocyte subsets in SS patient PBMCs after co-culture. Results: UC-MSCs reduced the proportions of peripheral blood CD3+ T cells, CD3+ CD8+ T cells, and CD19+ B cells in PBMC of SS patients, while the proportions of CD16+ CD56+ NK cells, CD3+ CD4+ T cells, and CD4+ /CD8+ ratio were not significantly affected. Conclusion: UC-MSCs can modulate the immune function of SS patients, thereby exerting a certain therapeutic effect on SS.
  • SUN Yang, JU Hongge, MA Qiang
    Journal of Baotou Medical College. 2024, 40(2): 93-96. https://doi.org/10.16833/j.cnki.jbmc.2024.02.017
    Objective: To investigate the clinical features, pathological features and differential diagnosis of Castleman’s disease of the retroperitoneum with strange-shaped cells, so as to reduce misdiagnosis. Methods: The clinical data of 1 case of Castleman 's disease of the retroperitoneum with strange-shaped cells admitted to our hospital were retrospectively analyzed and the literature was reviewed. Results: The patient came to the hospital with abdominal distension and discomfort for more than half a year. CT examination showed that a large retroperitoneal mass, and retroperitoneal tumor resection was considered for liposarcoma. Postoperative pathological examination showed that the number of lymphoid follicles increased, the mantle area widened, germinal center atrophy, a large number of plasma cells and a few lymphocytes between follicles in the background could be seen scattered strange-shape large cells. Immunohistochemical staining showed that CD3, CD5, CD38, CD20 and CD21 were all (+), and CyClinD1 (-), Kappa and Lambda were all (+). The patient was diagnosed as Castleman’s disease of the retroperitoneum, and was discharged from hospital with recovered well after surgery. After 9 months of follow-up, no obvious signs of recurrence were found. Conclusion: Castleman's disease with strange-shaped cells is rare, and the lack of understanding of strange-shaped cells can easily be misdiagnosed as malignant tumors, leading to overtreatment. The signs and imaging examinations are lack of specificity. Timely histopathological examination is the key to avoid misdiagnosis.