急性胰腺炎(acute pancreatitis, AP)是一种严重的消化系统疾病,其发生和发展与多种因素相关。近年来,肠道菌群失衡被认为是影响AP进程的重要因素。研究表明,AP患者的肠道菌群多样性减少、有益菌与致病菌比例失衡、特定菌群丰度的变化等,均通过影响肠道屏障功能和激发炎症反应影响AP进程。肠黏膜屏障在维持机体免疫防御系统完整性和正常生理功能方面起着至关重要的作用。肠道菌群失衡导致肠黏膜屏障功能受损,使肠道通透性增加,细菌发生易位,引发全身炎症反应综合征甚至多器官功能衰竭,加重胰腺炎病情,影响患者预后。此外,肠道菌群失衡还导致代谢产物的变化,如短链脂肪酸(short-chain fatty acids, SCFAs)和胆汁酸等,这些变化对胰腺功能和AP的发展有着重要影响。因此,维持肠道菌群平衡可能对AP的治疗和预后具有重要意义。笔者寻找对AP至关重要的特定菌株或参与AP的其他代谢产物,通过调节AP患者肠道菌群作为治疗AP的一种有效方法,深入研究肠道菌群与AP之间的相互作用,不仅有助于揭示AP的病理机制,还可能为AP的预防和治疗提供新的策略和方法。

Objective: To analyze the proportion of SPP1⁺ macrophages in peripheral blood of patients with connective tissue disease complicated with interstitial lung disease (CTD-ILD) and the expression level of its expression product chemokine 18 (CCL18) in serum of patients with CTD-ILD and its clinical significance. Methods: Twenty patients with connective tissue disease (CTD) and 20 healthy people were selected as the control group, and 35 patients with newly diagnosed CTD-ILD were selected as the experimental group. The differences in the expression levels of SPP1⁺ macrophages and CCL18 in the experimental group and the control group were compared, and the correlation with type I collagen (COL1A1), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) was analyzed. Results: Compared with the control group, the proportion of SPP1⁺ macrophages in peripheral blood mononuclear cells of CTD-ILD patients was significantly increased (P＜0.05), and the content of CCL18 in serum was also significantly increased (P＜0.05). At the same time, SPP1⁺ macrophages and CCL18 were positively correlated with ESR, CRP, COL1A1 and lung imaging score (HRCT score) (P＜0.05), and negatively correlated with the percentage of forced vital capacity (FVC%) and the percentage of carbon monoxide diffusion (DLCO%) (P＜0.05). The area under the curve (AUC) of SPP1⁺ macrophages and CCL18 were 0.732 and 0.939, respectively. The AUC of the combined detection of SPP1⁺ macrophages and CCL18 was 0.939, the sensitivity was 93.5%, the specificity was 83.3%, and the Youden index was 0.768. Conclusion: SPP1⁺ macrophages and CCL18 are poor prognostic factors in patients with CTD-ILD. The combined detection of SPP1⁺ macrophages and CCL18 is of great significance for the prognosis of the disease.

Objective: To investigate the active ingredients and mechanism of Sophora alopecuroides L. in the treatment of alcoholic liver disease (ALD) based on network pharmacology and molecular docking techniques. Methods: The active ingredients in Sophora alopecuroides L. were screened by Herb platform, and the corresponding targets of active ingredients were searched in SIB database. The targets of alcoholic liver disease were obtained from NCBI and GeneCards databases. The mapping tool Venny 2.1 was used for intersection comparison to obtain common targets. The protein interaction analysis and visual presentation were performed on the String platform. The DAVID database was used for GO function and KEGG pathway enrichment analysis. Finally, AutoDock and PyMOL software were used for molecular docking. Results: Eight active ingredients and 97 effective targets were identified by network pharmacology. The mechanism of action might be related to cancer pathway, lipid and atherosclerosis, T cell receptor signaling pathway and VEGF signaling pathway, and the main targets involved were HSP90AA1, AKT1, FYN and AKT2. Molecular docking results showed that matrine, sophocarpine and sophoramine had good binding force with HSP90AA1, FYN and AKT1. Conclusion: Matrine, sophocarpine and sophoramine in Sophora alopecuroides L. can ameliorate ALD by inhibiting inflammatory response or apoptosis through multi-target and multi-pathway interactions.

Objective: To observe the effects of ribokinase (Rbks) gene knockout on phenotype and glucose and lipid metabolism in C57BL/6J mice. Methods: 12 male 8-week-old wide type (WT) and Rbks gene knockout (KO) C57BL/6J mice were fed for 10 weeks. The changes of appearance, body weight, food intake, water intake and fasting blood glucose were observed regularly. At the end of the observation period, the contents of triglyceride and cholesterol were measured. Results: After being fed normally for 10 weeks, compared with WT mice, KO mice had no significant difference in appearance and body weight(P＞0.05), but food intake and water intake decreased, fasting blood glucose level increased significantly(P＜0.05), serum triglyceride content increased and total cholesterol content decreased (all P＜0.05). Conclusion: Although Rbks gene knockout has no effect on the shape and weight of mice, it will affect the balance of glucose and lipid metabolism in mice.

Objective: This article evaluates the biological safety of silver and stainless steel needles,which are used in Mongolian medicine.It provides scientific and technical support for their development and promotion. Methods: The experimental method followed the series of standards for biological evaluation of medical devices.Among them,Extraction standards for silver needle and stainless steel needle extracts followed the《Biological evaluation of medical devices - Part 12: Sample preparation and reference materials》(GB/T 16886.12-2023),Reference for extra-dermal and subcutaneous sensitization experiments followed the 《Biological evaluation of medical devices - Part 10: Irritation and skin sensitization tests》(GB/T 16886.10-2017),Blood compatibility test followed the《Biological evaluation of medical devices - Part 4: Selection of blood interaction tests》(GB/T 16886.4-2022),In this study, dermal and subcutaneous sensitization tests as well as blood compatibility tests were adopted to evaluate the biosafety of silver needles and stainless steel needles respectively.Male SD rats were used as experimental animals.The experimental needles were silver and stainless steel needles.The experimental solvent was needle extract and physiological saline.The general and local indicators,and the blood compatibility indicators of rats were observed in the experiment. Results: Silver and stainless steel needles did not cause obvious allergic reactions in rats in extracutaneous and subcutaneous sensitisation experiments,and did not affect the general and local indexes of rats.Silver and stainless steel needles did not cause any abnormalities such as blood coagulation,haemolysis and platelet aggregation in rats in the blood compatibility test. Conclusion: Silver and stainless steel needles are biocompatible and biologically effective,and have no significant adverse effects on the human body.They are suitable for Mongolian medical processes.

Objective: To optimize and enhance the quality standard of Yurijle-13 pill, a Mongolian medicinal preparation. Methods: :Microscopic identification was carried out on the powder of the formula encompassing Aucklandia lappa Decne, Gardenia jasminoides Ellis, Chrysanthemum morifolium Ramat, Punica granatum L, and Piper longum L in Yurijle-13 pill. Thin Layer Chromatography (TLC) was established for the identification of Aucklandia lappa Decne, Gardenia jasminoides Ellis, and Salvia miltiorrhiza Bge in Yurijle-13 pill. High Performance Liquid Chromatography (HPLC) was developed to concurrently determine the contents of the principal ingredient, Costunolide (CL), and Dehydrocostus lactone (DL) in the Aucklandia lappa Decne of Yurijle-13 pill by using a SinoPak SP analytical column (4.6 mm×250 mm, 5 μm), with a mixture of methanol and water (6535) as the mobile phase, a flow rate of 1.0 mL/min, a column temperature of 30 ℃, and a detection wavelength of 225 nm. Results: :The microscopic characteristics of the powder of Aucklandia lappa Decne, Gardenia jasminoides Ellis, Chrysanthemum morifolium Ramat, Punica granatum L, and Piper longum L in Yurijle-13 pill were distinct. Aucklandia lappa Decne, Gardenia jasminoides Ellis, and Salvia miltiorrhiza Bge demonstrated strong specificity in TLC qualitative identification. The linear regression equation for CL was y=315.83x-3.9247 (within the concentration range of 0.0051 to 0.2504 mg/mL), and that for DL was y=276.65x-5.5702 (within the concentration range of 0.0497 to 0.2494 mg/mL). The RSDs for the tests of precision, stability, and reproducibility were 1.13% and 1.17%, 3.43% and 3.04%, and 0.65% and 0.26%, respectively, and the average recovery rates were within the specified range (85.0% to 110.0%). Conclusion: :Compared with the Inner Mongolia Autonomous Region hospital preparation approved, the method for identification and content determination of Yourile-13 pill established in this study not only increase the types of identified medicinal materials and the main components of the drug, but also has the advantages of convenient operation, strong specificity and good repeatability, which is suitable for the quality control of Yourile-13 pill.

Objective: To investigate the impact of Mongolian medicine saorilao-4 (SRL-4) on miRNA and core genes involved in the gene regulatory network of lung tissue in rats with pulmonary fibrosis (PF). Methods: Rats were randomly divided into four groups: blank control group (CON), model group (MOD), positive drug control group, and SRL-4 group. Except for the CON group, rats in the other groups were administered with bleomycin via intratracheal injection to establish a pulmonary fibrosis model. The total RNA was extracted from rat lung tissue for transcriptome sequencing. The differentially expressed miRNA (DEM) in each group was screened by the difference analysis software edge R. The differentially expressed gene (DEG) of DEM was predicted by miRanda. The GO and KEGG were used to analyze the biological function enrichment of DEG. Cytoscape was used to construct the target gene regulatory network and screen the core genes. Results: Compared with CON, MOD selected 16 DEMs. Compared with MOD, SRL-4 screened 10 DEMs and regulated 63 052 target genes. GO analysis showed that the DEGs of SRL-4 and MOD were enriched in 52 GO entries. KEGG analysis showed that DEG was enriched in 182 signaling pathways, among which the number of genes related to purine metabolic pathway was the highest. By constructing a gene regulatory network, six core genes were screened, namely Spata25, Sultan1a1, Mpv17i, Cryba4, Jakmip3 and Fkbp5. Conclusion: By constructing a miRNA-Target regulatory network between SRL-4 and MOD, we identified six hub genes that are considered key molecules in the gene regulatory network for the treatment of PF. The improvement effect of SRL-4 on PF may be related to miR-433-3p, novel_202, miR-150-3p, and these 6 core genes. The purine metabolism-related signaling pathway may be a critical target and essential pathway for the treatment of PF.

随着中药病理学研究的不断发展,中药在治疗变应性鼻炎方面的理论日益充实且成效显著。近年来,变应性鼻炎的机制研究取得了长足的进展,其中自噬及其通路更成为该领域的热门研究方向。本文通过对细胞自噬发挥治疗作用的中药单体、方剂及其调控通路进行总结归纳与系统分析,以期明确其中的生物学规律和特点,为后期更深入的研究提供参考。

Objective: To analyze the epidemiological characteristics, etiological surveillance, and clustering epidemic of hand-foot-mouth disease (HFMD) in Jinniu district of Chengdu from 2017 to 2023, so as to provide basis for the prevention and control strategy of HFMD. Methods: Descriptive epidemiological research methods were used to analyze the HFMD surveillance data in Jinniu district of Chengdu, from 2017 to 2023. Results: From 2017 to 2023, a total 12 960 cases of HFMD were reported in Jinniu district, with an annual incidence rate of 64.05/100 000 to 305.95/100 000; except for a few years, the number of began to increase in May each year, with the overall seasonal epidemic peaks occurring from May to July and from September to November. The male-to-female ratio of cases was 1.4∶1; the cases were mainly concentrated in children aged 0-＜6 years, accounting for 90.75% of the total number of cases; the most cases were scattered children, followed by children in kindergartens, accounting for 92.93% of the reported cases. The results of enterovirus monitoring showed that other enteroviruses, CVA16, and CVA6 were the main pathogens accounting for 59.75%, 15.56%, and 14.57%, respectively. The clustering epidemic was mainly concentrated from to July and from September to November, mainly occurring in kindergartens, accounting for 96.08% of the total; events with 2-5 cases were the most common, accounting for 93.56%. Conclusion: The prevention and control of HFMD should focus on young and scattered children, a particular emphasis on the changing dominant strains causing the disease. Outbreaks in Jinniu district mainly occurred in kindergartens, especially from May to and from September to November, during which targeted measures should be taken to reduce the occurrence and spread of the epidemic.

Objective: To investigate the role of logistic prediction model based on two-dimensional ultrasound image features to assess the risk of BRAF V600E mutation in TI-RADS 4a thyroid nodules. Methods: Patients with TI-RADS category 4a thyroid nodules who underwent post-puncture BRAF V600E gene testing at Yijishan Hospital, Wannan Medical College from January 2021 to September 2021 were collected, and data on ultrasound, pathology and gender and age of selected patients were obtained for retrospective analysis. The patients were divided into the BRAF V600E positive group(n=130)and BRAF V600E negative group(n=104)according to the gene test results. Multiple logistic regression models of two-dimensional ultrasound were constructed based on ultrasound image characteristics. The predictive efficacies of the models were assessed by ROC(receiver operating characteristic)curves and calibration curves, and the area under curve AUC was compared between the models using the Delong test. Results: The AUC for the training set obtained from the logistic echo model was 0.885 5(95%CI: 0.833 8-0.937 2), with specificity, sensitivity and accuracy of 67.0%, 96.0% and 83.0% respectively. The AUC of the validation set was 0.859 3(95%CI: 0.771 9-0.946 7), with specificity, sensitivity and accuracy of 79.0%, 84.0% and 82.0% respectively. Delong test results showed no statistically significant difference in AUC between the training and validation set models(P＞0.05). good clinical efficacy of the prediction models(total point 0.9)was showed by column plots. The calibration curve showed high agreement between the actual and predicted probabilities(C-index 0.9). Conclusion: Logistic regression models constructed based on 2D ultrasound image features can be used as a simple and non-invasive quantitative tool to predict the risk of BRAF V600E mutation in TI-RADS 4a thyroid nodules.

Objective: To investigate the protective effect of ursolic acid (UA) on alcoholic liver damage (ALD) mice by observing the expression of bile salt export pump (BSEP) and sodium taurocholate cotransporting polypeptide (NTCP) in mice after UA intervention. Methods: Eighteen 10-week-old SPF C57BL/6J mice were randomly divided into three groups (normal control group, alcohol model group, and UA+alcohol group). The mice were treated with intragastric administration for 7 weeks. The changes of body weight were recorded. The pathological changes of liver tissue were observed by HE staining. The serum total bile acid (TBA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected by microplate method. The expression of BSEP and NTCP protein in liver tissue was detected by Western blotting. Results: The average body weight of mice in the alcohol group was significantly lower than that in the normal control group (P＜0.05). The structure of hepatic lobules in the normal control group was intact and there was no inflammatory cell infiltration, while the structure of hepatic lobules in the alcohol model group was blurred, accompanied by obvious steatosis, and the NAS score was significantly increased (P＜0.05). Compared with the alcohol model group, after UA intervention, the damage of hepatic lobule structure in mice was improved, the number of fat vacuoles and inflammatory cells in hepatocytes was reduced, and the NAS score was significantly reduced (P＜0.05). In addition, compared with the normal control group, the liver index of the alcohol model group was significantly increased (P＜0.05), while the liver index of the UA+alcohol group was significantly lower than that of the alcohol model group (P＜0.05). At the same time, after UA intervention, the increase of TBA, AST and ALT induced by alcohol intake was significantly inhibited (P＜0.05). The results of Western blotting showed that compared with the normal control group, the expression levels of BSEP and NTCP in the liver tissue of the alcohol model group were significantly decreased (P＜0.05). After UA intervention, the expression levels of transporter BSEP and NTCP protein in UA+alcohol group were significantly increased (P＜0.05). Conclusion: UA supplementation can significantly improve liver damage caused by alcohol, and the mechanism may be related to the up-regulation of BSEP and NTCP protein expression, thereby regulating bile acid homeostasis.

Objective: To observe the effect of Mongolian medicine total flavonoids of Dracocephalum moldavica L (TFDM) on the expression of microtubule-associated protein-2 (MAP-2) in the ischemic penumbra of rats after cerebral ischemia-reperfusion, and explore its role in neural plasticity. Methods: Constructing a middle cerebral artery occlusion reperfusion model using SD rats. The experiment was divided into sham surgery group (Sham), ischemia-reperfusion group (I/R), and TFDM administration group (TFDM). Samples were collected at four time points: 1, 3, 7, 14 days after surgery. The immunohistochemical staining was used to observe the expression of MAP-2 positive labeled cells and dendrites in the ischemic penumbra of the cortex, and Western blot was used to detect the expression of MAP-2 protein in the ischemic penumbra of the cortex. Results: The results of immunohistochemistry and Western Blot showed that the expression of MAP-2 in the ischemic penumbra of the TFDM group was significantly higher than that in the I/R group on the 3rd and 7th day after reperfusion (P＜0.05). Conclusion: TFDM may play a neuroprotective role by up-regulating the expression of MAP-2 protein, enhancing the regulation of neural plasticity in the ischemic penumbra, promoting dendritic growth and repairing damaged nerve structure.

Objective: To analyze the expression level and the correlation with clinical data of type X collagen α 1 chain (COL10A1) in the serum of breast cancer patients, so as to evaluate its predictive value for breast cancer risk. Methods: GEPIA database was used to analyze the expression of COL10A1 in breast cancer patients. The expression of COL10A1 in the serum of 30 breast cancer patients was detected by PCR, and its relationship with the clinical data of patients was analyzed. ROC curve was used to analyze the clinical diagnostic value of COL10A1 in breast cancer. The potential targets of COL10A1-mediated breast cancer were obtained through the Genecards database and OMIM database, and they were input into the STRING database to obtain the TSV file of the protein-protein interaction (PPI) network. GO enrichment analysis and KEGG enrichment analysis were used to explore different functional categories and related signal transduction pathways. Results: Bioinformatics analysis and PCR results showed that COL10A1 was highly expressed in breast cancer, which was closely related to histological grade, TNM stage, distant lymph node metastasis and Her2. ROC curve showed that the area under the curve of COL10A1 was 0.690 0 (95%CI: 0.5567-0.8233). When the optimal cut-off value was 1.1026, the sensitivity and specificity of COL10A1 in the diagnosis of breast cancer were 60.0% and 68.9%, respectively. A total of 14 potential targets of COL10A1-mediated breast cancer were obtained by combining the Genecards database with the OMIM database. A total of 241 GO terms were enriched by GO analysis, including 9 cellular components, 48 biological processes, and 12 molecular functions. KEGG enrichment analysis enriched a total of 12 signal transduction pathways. Conclusion: The high expression of serum COL10A1 in breast cancer patients is closely related to clinicopathological indicators, and has certain clinical value for breast cancer risk assessment.

Objective: To explore the value of nucleic acid detection of pathogenic bacteria in lower respiratory tract in the diagnosis of lower respiratory tract infection. Methods: Patients who underwent nucleic acid testing and bacterial culture for lower respiratory pathogens from December 2023 to November 2024 were selected as subjects. The detection rates of respiratory pathogens by both methods were compared. Results: For six common pathogens causing lower respiratory infections, the positive detection rate of nucleic acid testing was significantly higher than that of conventional bacterial culture (69.68% vs.20.88%). For Pseudomonas aeruginosa, the two methods had good consistency in positive detection rates (Kappa=0.644, P＜0.001), while the consistency of detection rate of klebsiella pneumoniae between the two methods was general (Kappa=0.473, P＜0.001), and that of staphylococcus aureus was poor (Kappa=0.287, P＜0.001). The average turnaround time within the laboratory for nucleic acid testing was 4.68 hours, while that for conventional bacterial culture was 49.75 hours, the average turnaround time of nucleic acid detection in the laboratory was significantly lower than that of bacterial culture (P＜0.001). Conclusion: Nucleic acid detection of pathogenic bacteria in lower respiratory tract has more advantages in rapid identification of pathogenic bacteria in respiratory tract, with shorter laboratory turnaround time, can provide timely information on pathogenic bacteria for clinicians, and has higher clinical significance for patients with severe and rapid progress.

Objective: To compare the efficacy of 3% hypertonic saline(HTS) and 10% HTS combining with 20% mannitol(MT) in the treatment of high cranial pressure after acute massive cerebral infarction. Methods: A total of 40 patients with acute massive cerebral infarction complicated with intracranial hypertension diagnosed and treated by the inpatient Department of Encephalopathy, Guilin Hospital of Traditional Chinese Medicine from 2020 to 2023 were selected in this study. Selected patients were divided into two groups(20 patients in each group) according to random number table method. The treatment group(groupA) was treated with 10% HTS 60 mL and 20% MT 125 mL at alternate static points for cranial pressure reduction(the specific frequency was referred to the adjustment of intracranial pressure), and the control group(groupB) was treated with 130 mL of 3% HTS and 125 mL of 20% MT sequentially. Intracranial pressure(ICP), mean arterial pressure(MAP) and central venous pressure(CVP) were continuously monitored before and after medication in both groups, and the corresponding cerebral perfusion pressure(CPP) was calculated according to ICP and MAP. The duration of effective reduction of intracranial pressure, the maximum reduction and duration of intracranial pressure in enrolled patients were recorded, and the blood sodium value and plasma osmotic pressure were recorded before, 2 and 6 hours after medication. Results: A total of 40 cases were enrolled, with 36 cases completed and 4 cases rejected. The statistical results showed that ICP, MAP, CPP, blood sodium and osmotic pressure were significantly different between the two groups before and after treatment in different periods(P＜0.05). After 2 and 6 h of medication, ICP in groupA was lower than that in groupB(P＜0.05), while MAP, CVP, CPP, sodium and plasma osmotic pressure were higher than those in the control group(P＜0.05). ICP was the most significantly decreased 2 h after treatment(P＜0.05), and ICP was the most significantly decreased in the range of 150 to 160 mmol/L of blood sodium(P＜0.05). Conclusion: 3% and 10% HTS can effectively reduce ICP in patients with acute large area cerebral infarction, but 10% HTS has more lasting effect on reducing intracranial pressure and less influence on blood sodium and internal environment, which is worthy of clinical promotion.

Objective: To investigate the expression of miR-27a-3p in chronic intermittent hypoxia (CIH) and its involvement in insulin resistance through biological analysis. Methods: Download the miRNA expression dataset under CIH conditions from GEO, use the EdgeR package of R software for differential miRNA screening, and select differential miR-27a-3p as the research object. Using Target Scan, miRRisk, and miRDB to predict the target genes of miR-27a-3p and obtain the intersection for GO and KEGG enrichment analysis. The gene set of insulin resistance was retrieved through genecard, and the intersection of miR-27a-3p target genes was taken. Then, a PPI network of miR-27a-3p target genes was constructed, central genes were screened, and a miRNA mRNA pathway regulatory network was constructed. Results: a total of 37 differentially expressed miRNAs were screened from the GSE210021 dataset, among which miR-27a-3p was one of the down regulated genes. There are 505 intersecting target genes of miR-27a-3p in three databases, mainly involved in the regulation of protein serine/threonine kinase activity and other biological processes, as well as the MAPK signaling pathway. There are 137 intersecting target genes between the insulin resistance gene set and miR-27a-3p, including central genes such as EGFR. The miRNA mRNA pathway shows that miR-27a-3p is a key regulatory factor in the MAPK signaling pathway and other pathways. Conclusion: Under chronic intermittent hypoxia, miR-27a-3p expression is downregulated, which may be involved in the occurrence of insulin resistance by acting on target genes such as MAPK14. The mechanism may involve related signaling pathways of MAPK and other targets.

家庭参与式护理模式(family integrated care, FICare)具备促进早产儿成长发育、减轻早产儿父母负面情绪以及提升早产儿父母育儿照护能力等优势。本文详细阐述了FICare的起源、定义、特点、应用状况、干预内容以及实施方法,并针对我国新生儿监护病房(neonatal intensive care unit, NICU)所面临的挑战,提出了相应的建议与展望,旨在为国内早产儿护理提供参考依据。

Objective: To investigate the early effect of Kinesio taping combined with rehabilitation training on patients with anterior and posterior cruciate ligament reconstruction under arthroscopy. Methods: A retrospective analysis of 40 patients (40 knees) who underwent arthroscopic anterior and posterior cruciate ligament reconstruction in the First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology from May 2013 to April 2023, including 28 males and 12 females, aged 18-50 years, with an average age of 32.5 years; the time from injury to treatment was 1-12 days, with an average of 5.9 days, and the time from injury to operation was 8-21 days, with an average of 15.1 days; among them, 20 patients receiving traditional rehabilitation were defined as the control group, and 20 patients receiving traditional rehabilitation+Kinesio taping were defined as the experimental group. The pain degree of the affected limbs of the two groups was evaluated by Visual Analogue Scale (VAS) before operation and 5 days, 2, 4, 6 and 8 weeks after operation. The swelling degree of the knee joint of the two groups was evaluated by the difference between the circumference of 5 cm above the patella of the affected limb and the circumference of the same position on the healthy side. Lysholm score was used to evaluate the recovery of knee joint function after operation. Berg scale was used to evaluate the recovery of limb balance ability in the two groups. The recovery of proprioception of the affected limbs of the two groups was evaluated by active flexion position recurrence method. Results: All 40 patients were followed up after operation. The VAS score of the affected limb in the experimental group was lower than that in the control group at 2 weeks after operation (P＜0.05), the improvement of swelling degree was better than that in the control group at 4 weeks after operation (P＜0.01), the Lysholm score was gradually higher than that in the control group at 4 weeks after operation (P＜0.05), the Berg score was better than that in the control group at 6 weeks after operation (P＜0.01), and the recovery of proprioception of the affected limb was better than that in the control group at 8 weeks after operation (P＜0.05). Conclusion: Kinesio taping can effectively relieve early limb pain and swelling in patients with anterior and posterior cruciate ligament rupture after arthroscopic reconstruction, promote the recovery of knee joint balance and proprioception, and improve knee joint function.

Objective: To analyze the correlation between the levels of serum transcription activator factor 3 (ATF3), tumor necrosis factor receptor 1 (TNFR1), angiopoietin-like protein 4 (ANGPTL4) and the renal function, prognosis of patients with diabetic nephropathy. Methods: A total of 152 patients with diabetic nephropathy who were treated in the Second People's Hospital of Jiaozuo City from April 2022 to January 2024 were selected as the study group, and 152 patients with simple diabetes who were treated in the same period were selected as the control group. The levels of serum ATF3, TNFR1, ANGPTL4 and renal function indexes such as serum calcium, serum phosphorus, serum creatinine and glomerular filtration rate (eGFR) were compared between the two groups at admission, and their correlation was analyzed. The levels of serum ATF3, TNFR1 and ANGPTL4 in patients with different prognosis were compared at admission, and their correlation with prognosis and predictive value for prognosis were analyzed by partial regression. Results: Compared with the control group, the levels of ATF3, TNFR1, and ANGPTL4 in the study group were significantly higher at admission (P＜0.05). The levels of blood calcium and creatinine in the study group were higher than those in the control group, while the levels of blood phosphorus and eGFR were lower than those in the control group (P＜0.05). Serum ATF3, ANGPTL4, and TNFR1 were negatively correlated with blood phosphorus and eGFR, and positively correlated with blood calcium and creatinine levels (P＜0.05). In the study group, patients with poor prognosis had higher levels of ATF3, ANGPTL4, and TNFR1 on admission than patients with good prognosis (P＜0.05). Logistic regression analysis showed that serum ATF3, ANGPTL4 and TNFR1 were risk factors for poor prognosis of patients (P＜0.05). The efficacy of combined prediction of serum ATF3, TNFR1 and ANGPTL4 levels on admission in predicting poor prognosis of patients was 0.807, which was higher than that of each index alone (P＜0.05). Conclusion: The increase in serum levels of ATF3, TNFR1, and ANGPTL4 in patients with diabetic nephropathy is associated with their renal function. Clinical detection of these levels can help predict the prognosis of patients and provide effective reference for clinical diagnosis and treatment.

Objective: To investigate and analyze the efficacy of high-flow nasal cannula oxygen therapy in the sequential treatment of mechanical ventilation weaning for pneumonia complicated with type I respiratory failure. Methods: A total of 70 patients with pneumonia and type I respiratory failure admitted from January 2023 to December 2024 were divided into experimental group and control group. All patients were treated with mechanical ventilation. The experimental group was treated with high-flow nasal cannula oxygen therapy, and the control group was treated with non-invasive positive pressure ventilation. The clinical efficacy, blood gas analysis indexes (before treatment, 12, 24 and 48 hours after treatment) and sputum traits, inflammation index levels (before treatment and 3 days after treatment), sequential treatment time, total hospitalization time and reintubation mechanical ventilation rate were compared between the two groups. Results: The total effective rate of clinical efficacy in the experimental group was higher than that in the control group (P＜0.05). There were no statistically significant differences in PaO₂, PaCO₂, and SaO₂ levels between the two groups before treatment and at 12, 24, and 48 hours after treatment (P＞0.05). There was no significant difference in PaO₂, PaCO₂ and SaO₂ levels between the two groups before treatment and 12,24 and 48 hours after treatment (P＞0.05). There was no difference in CRP and PCT levels between the two groups before treatment (P＞0.05). After 3 days of treatment, CRP and PCT in the experimental group were lower than those in the control group (P＜0.05). There was no significant difference in sputum traits between the two groups before treatment (P＞0.05). After 12, 24 and 48 hours of treatment, the sputum traits I of the experimental group were better than those of the control group (P＜0.05). The sequential treatment time and total hospitalization time of the experimental group were lower than those of the control group (P＜0.05). Conclusion: High-flow nasal cannula oxygen therapy can improve the clinical efficacy of patients with pneumonia complicated with type I respiratory failure after mechanical ventilation weaning, dilute sputum, improve oxygenation, reduce inflammation, improve patient tolerance, reduce the risk of re-intubation mechanical ventilation due to intolerance, shorten the treatment time, and promote its rehabilitation. It is worthy of application.