Objective: To establish an osteogenic model of MC3T3-E1 cells by chemical induction, to explore the relationship between FAK gene expression and osteoblast formation, and to understand the effect of FAK on osteogenic differentiation. Methods: The cell morphology of MC3T3-E1 cells after osteogenesis induction was observed, mineralization was detected by alizarin red chemical staining, and the expression of osteogenic markers Runx2 and ALP was detected by real-time fluorescence quantitative PCR, while the expression of FAK in the osteogenesis process was analyzed. Results: MC3T3-E1 cells showed long shuttle or polygonal shape after 3 d of osteogenesis induction; mineralized nodules were visible by alizarin red staining at 21 d and 28 d. The expression of osteogenic markers Runx2 and ALP showed a continuous increase, and the expression of FAK showed an overall increasing trend, with a slight decrease at 7 d. Conclusion: The expression of FAK was basically consistent with the expression trend of osteogenesis-related genes, suggesting that FAK may play a role in the osteogenesis process of MC3T3-E1 cells.

Objective: To evaluate the marginal and internal suitability of temporary crowns of different materials made by direct or indirect methods.Methods: 40 patients with full crown restoration of the first molars in the outpatient department of Anhui Medical College from January 2021 to January 2022 were selected and divided into 4 group with 10 crowns each group.A stereo microscope was used to compare the differences in the edge and internal fit of the 4 sets of temporary crowns.Results: The average gap of temporary crowns made of different materials was 184.27-222.40 μm.The indirect CAD/CAM resin temporary crown set has the smallest margin and internal clearance, and it conforms to the normal distribution. The edge and internal clearance of the PMMA resin temporary crown made by the direct method were larger than that of the PMMA full crown made by the indirect method (P＜0.05). Conclusion: The indirect method CAD/CAM resin temporary crown has higher accuracy, followed by Protemp resin,the PMMA temporary crown prepared by the direct method has the maximum edge and internal clearance.

Wnt蛋白家族属于一类具有高度保守半胱氨酸残基的分泌性脂质修饰糖蛋白,其通路是一组高度保守的信号转导通路,能够介导多种生理和病理过程,如参与调控细胞增殖、分化、凋亡、免疫与应激、炎症和纤维化。目前认为Wnt信号通路包括三个分支:经典Wnt信号通路,即Wnt/β-Catenin信号通路;Wnt/PCP通路;Wnt/Ca²⁺通路。Wnt/β-catenin信号途径在心、肝、肺、肾等器官纤维化等病理生理过程中均发挥了重要作用。越来越多的证据表明,Wnt/β-catenin信号通路在糖尿病肾病(diabetic nephropathy, DN)的进展中意义重大。本文就Wnt/β-catenin在系膜细胞凋亡、上皮间充质转变(epithelial mesenchymal transition, EMT)、DN条件下足细胞功能障碍、小管EMT和肾纤维化中的作用进行综述。此外,还介绍了这一过程所涉及的分子机制。这一信息为Wnt/β-catenin保护肾脏损伤和延缓DN进展提供了一个新的分子靶点。

Objective: To clarify whether general cistanosides (GCs) ameliorates cognitive dysfunction in rapidly aging mice (SAMP8) by mediating specific androgen receptor (AR) in regulation of expression of synaptic protein. Methods: A total of 76 7-month-old male SAMP8 mice were randomly divided into the model group, GCs group, GCs + flutamide (F) group, GCs + fulvestrant (ICI) group, F group, and ICI group. The learning and memory functions of mice in each group were tested with Morris water maze. Western blotting and RT-PCR were used to test the expression level of synaptophysin (SYN), postsynaptic density-95 (PSD-95) and the brain-derived neurotrophic factor (BDNF) of mice in each group. Results: GCs significantly improved the learning and cognitive function of SAMP8 mice. Comparing with the GCs group, the learning and memory function of mice in the GCs+F group and GCs+ICI group was significantly lowered. Western blotting and RT-PCR results showed that the expression level of BDNF, SYN, and PSD-95 were significantly increased at the protein level and gene level in the GCs group compared with the Model group (P＜0.05). SYN expression level was increased in the GCs+F and GCs+ICI group, and expression levels of BDNF and PSD-95 were significantly decreased in the GCs+F and GCs+ICI group compared with the GCs group (P＜0.05). Conclusion: GCs may specifically mediate AR to enhance synaptic plasticity in SAMP8 mice, which in turn improve learning and cognitive function.

白血病是一类起源于造血系统并具有高度异质性的恶性克隆性疾病。三氧化二砷(arsenic trioxide, ATO, As₂O₃)是治疗急性早幼粒细胞性白血病(acute promyelocytic leukemia, APL)和其他类型白血病的重要药物。ATO治疗APL效果最为显著,可通过多种机制发挥作用,包括诱导细胞凋亡和自噬、抑制细胞生长等,同时ATO联合其他药物治疗白血病也取得了很大的研究进展。然而,虽然ATO治疗白血病有很好的疗效,但其具有全身细胞毒性作用,近年来研究学者致力于采用纳米技术靶向递送ATO和其他药物以降低其毒性作用的研究已取得一定的成果。

Objective: To compare the short-term pain response and long-term clinical efficacy of iRoot SP and AH-PLUS as apical sealers. Methods: A total of 96 single-rooted permanent teeth of 90 patients with chronic periapical periodontitis from August 2017 to December 2018 were randomly selected as the study objects. After routine preparation of root canal, they were divided into the iRoot SP group and AH-PLUS group based on different apical sealers they used. The pain response of the two groups 1 week after surgery was compared, and clinical efficacy 6 months after surgery of the two groups were compared. Results: The pain degree in the AH-PLUS group was slightly higher than that in iRoot SP group 1 week after surgery, and the difference was statistically significant (P＜0.05). The accurate filling rate in the iRoot SP group was higher than that in the AH-PLUS group (P＜0.05). Six months after root canal filling, there was a statistically significant difference between the two groups in the treatment effect (P＜0.05). The success rate of the iRoot SP group was higher than that of the AH-PLUS group (P＜0.05). Conclusion: iRoot SP has less pain stimulation to periapical tissues 1 week after surgery, better filling effect and long-term clinical efficacy than the AH-PLUS, it could be widely used in clinical practice.

Objective: To explore the expression and clinical significance of THBS2 and Survivin in colorectal cancer. Methods: Paraffin-embedded colorectal cancer tissues and 20 paracancer tissues of 60 patients with complete medical records meeting the inclusion criteria were selected as colorectal cancer group and colorectal cancer group, and THBS2 and Survivin expression were detected by immunohistochemical staining. The pathological data and medical records of 60 patients with colorectal cancer were collected, and the survival follow-up of 30 patients with colorectal cancer was obtained. Spearman detection and Chi-square detection were performed to analyze the correlation of THBS2 and Survivin with different pathological parameters, and survival curves of THBS2 and Survivin with different expression parameters were plotted. Results: The positive expression rate of THBS2 was 80.0 % in colorectal cancer tissues, 10.0 % in adjacent tissues, 83.3 % in colorectal cancer tissues, and 5.0 % in adjacent tissues. The expressions of THBS2 and Survivin were statistically significant in colon cancer tissues and adjacent tissues (P＜0.01), and the expressions of THBS2 and Survivin were statistically significant in lymph node metastasis, degree of tumor differentiation, and AJCC eighth stage of colorectal cancer (P＜0.05). The expressions of THBS2 and Survivin were not significantly different with age, gender, pathological type, liver metastasis, tumor site and tumor size (P＞0.05). THBS2 expression was positively correlated with Survivin expression (r=0.447, P＜0.05). Conclusion: The differential expression of THBS2 and Survivin in colorectal cancer is related to the occurrence and development of colorectal cancer.

Objective: To observe the effects of curcumin on mitochondrial autophagy and oxidative stress in human renal tubular epithelial cells (HK-2) under high glucose environment, and to explore the protective effect and mechanism of curcumin on HK-2 cells. Methods: HK-2 cells were divided into 4 groups: control group(5.5 mmol/L glucose), curcumin group(5.5 mmol/L glucose +10 μmol/L Cur), high glucose group(30 mmol/L glucose) and curcumin high glucose group(30 mmol/L glucose+10 μmol/L Cur). All groups were stimulated for 48h. Total protein was collected, and the expression of mitochondrial autophagy related proteins Pink1, Parkin, LC3B and beclin1 was detected by Western blot. Mitochondrial membrane potential was detected by JC-1. Reactive oxygen species (ROS) detection kit was used to detect intracellular ROS production. Results: Compared with the control group, the indexes in the curcumin group did not change significantly(P＞0.05). Compared with curcumin group, the protein expressions of Pink1, Parkin, LC3B and Beclin1 in high glucose group were significantly decreased(P＜0.01), intracellular ROS expression increased significantly(P＜0.01), and mitochondrial membrane potential activity decreased(P＜0.01). Compared with high glucose group, the protein expression of Pink1, Parkin, LC3B and Beclin1 in curcumin high glucose group was significantly increased(P＜0.01), intracellular ROS expression was significantly decreased(P＜0.01), mitochondrial membrane potential activity increased(P＜0.01). Conclusion: Curcumin can reverse the mitochondrial autophagy induced by high glucose and inhibit oxidative stress in HK-2 cells.

Objective: To observe the regenerative effect of Mongolian medicine Eerdun Wurile (EW) on zebrafish heart injury and its possible mechanism, and provide experimental basis for further research on Mongolian medicine Eerdun Wurile. Methods: The animal model of ventricular injury was established using the transgenic line Tg (vmhc: mCherry) treated with metronidazole (MTZ). The zebrafish embryos at 3 dpf(days post fertilization) were randomly divided into the control group (DMSO group,n=30), the experimental myocardial ablation group (MTZ group, n=30) and the Eerdun Wurile treatment group (MTZ+EW, n=30) after ventricular injury. Three groups were treated with 25 ug/ml Eerdun Wurile, a Mongolian medicine, after MTZ treatment for 24 hours. Three groups were tested for cell proliferation at 72 hpt. Cell proliferation were observed with confocal microscope and quantitatively analyzed. At the same time, NOS, iNOS, TNF-α, IL-1β and IL-6 were analyzed with real time qPCR. Results: After the treatment with Mongolian medicine Erden-Uhelle, the proportion of regeneration and repair after myocardial injury was significantly increased in the treatment group. And the proliferation of regenerated myocardial cells was enhanced in Eerdun Wurile treated group. The expression of inflammatory factors was significantly lower than that of the control group. Conclusion: Mongolian medicine Eerdun Wurile can promote the repair and regeneration of zebrafish embryonic cardiac muscle cells, promote the proliferation of cardiac muscle cells, and inhibit the inflammatory reaction after zebrafish embryonic ventricular injury.

Objective: To investigate the neuroprotective effect of ginsenoside Rb1 (GsRb1) on Parkinson's disease (PD) model mice induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) and its dose-effect relationship. Methods: Fifty C57BL/6 mice were randomly divided into five groups: control group, MPTP group, GsRb1 treatment group (low-dose group, middle dose group and high-dose group), with 10 mice in each group. The model group and the treatment group were injected MPTP (30 mg/kg) into the peritoneal cavity continuously for 5 days to prepare the PD mouse model. The treatment group received intraperitoneal injection of GsRb1 at doses of 10 mg/kg, 20 mg/kg and 40mg/kg respectively every day. The control group was given the same amount of normal saline. On the second day after administration, the behavioral changes of the mice were detected by using Rotarod test, Pole-climbing test and Grasping test. Results: Compared with the control group, the latent period of MPTP group mice was significantly reduced (P＜0.01), the climbing time was significantly increased (P＜0.01), and the Graspiing test score was significantly reduced (P＜0.01). Compared with the MPTP group, the latent period in the middle and high dose groups increased significantly (P＜0.05), the climbing time in low and middle dose groups decreased significantly (P＜0.05) and the scores of Graspiing test in middle and high dose groups were significantly higher (P＜0.05). Conclusion: GsRb1 has neuroprotective effect on MPT-induced PD model mice, and 20 mg/kg/d has a better effect.

Objective: To investigate the diagnostic value of peripheral blood neutrophil CD64 index (nCD64IND), monocyte CD14 index (mCD14IND) and interleukin 6 (IL-6) levels in adult pulmonary infection. Methods: A total of 63 adult patients with pulmonary infection hospitalized from December 2020 to February 2021 were selected as the study group and 47 healthy adults as the control group. The levels of nCD64IND, mCD14IND and IL-6 in peripheral blood were determined by flow cytometry. Pearson's method was used to analyze the correlation between the three indices and the common indicators of pulmonary infection, such as CRP and PCT. The ROC curve was drawn to evaluate the sensitivity and specificity of the three indices in the diagnosis of pulmonary infection. Results: The levels of nCD64IND, mCD14IND and IL-6 in peripheral blood of adult patients with pulmonary infection were significantly higher than those of healthy control group (all P＜0.05). Correlation analysis showed that the levels of nCD64IND and IL-6 in peripheral blood of patients were positively correlated with CRP, mCD14IND was negatively correlated with CRP, and the three indices were not significantly correlated with PCT. ROC curve analysis showed that the sensitivity of nCD64IND, mCD14IND and IL-6 levels in the diagnosis of lung infection was 81 %, 58.7 % and 84.1 %, respectively, and the specificity was 89.4 %, 78.7 % and 89.4 %, respectively. Conclusion: The determination of nCD64IND, mCD14IND and IL-6 in peripheral blood has certain diagnostic value for pulmonary infection.

Objective: To investigate the relationship between serum fibroblasts growth factor (FGF) 19 level and the development of atherosclerosis (AS) in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 78 T2DM patients were selected and divided into two groups based on carotid intima-media thickness (CIMT). Among them, 40 T2DM patients were control group and 38 T2DM patients with AS were experimental group. Record the blood pressure and body mass index (BMI) of all inclusive patients. Detection of related biochemical indicators such as blood sugar, blood lipids. Serum FGF19, heme oxygenase-1 (HO-1),malondialdehyde (MDA), glutathione peroxidase (GSH-Px) levels were measured by enzyme-linked adsorption immunosorbent assay (ELISA), and superoxide peroxide dismutase (SOD) level was determined by biochemical method. The differences in outcome measures between the two sets were compared. The correlations between FGF19 level and HO-1, SOD, MDA, GSH-Px, CIMT and brachial ankle pulse wave velocity (baPWV) were analyzed by Pearson correlation analysis. Multiple linear regression analysis was performed to analyze the influencing factors of FGF19. Results: Compared with the control group, CIMT, baPWV, HO-1, MDA increased in the experimental group (P＜0.01), FGF19, SOD and GSH-Px decreased significantly compared with the control group (P＜0.01). Correlation analysis showed that the serum FGF19 level of the experimental group was negatively correlated with HO-1, MDA, CIM, baPWV(r=-0.668、-0.657、-0.901、-0.734,P＜0.01) were positively correlated with SOD and GSH-px (r=0.345、0.760,P＜0.01).Multiple linear regression analysis showed that FGF19, HO-1, SOD, MDA, GSH-PX were all the possible influencing factors of CIMT (P＜0.05). Conclusion: Serum FGF19 level in T2DM patients has a certain relationship with oxidative stress, and has important clinical significance in predicting the occurrence and progression of AS.

Rheumatoid arthritis is a chronic systemic autoimmune disease. The inflammatory response caused by the abnormal activation of the immune system will lead to the formation of pannus in the synovium of the joints, and the gradual destruction of bone and cartilage. The clinical manifestations are pain and swelling of multiple joints, or even deformity, affect the quality of life of patients, and eventually lead to loss of joint function. The main reason for its onset and recurrence is that the immune system produces immune cells, cytokines and antibodies against self-antigens, among which the NLRP3 inflammasome plays a key role in the inflammatory response. As a binding protein of NLRP3, NEK7 kinase acts downstream of K⁺ efflux and regulates the oligomerization and activation of NLRP3 inflammasomes. However, there have been no report on the involvement of NEK7 in the inflammatory activities of rheumatoid arthritis through the activation of NLRP3 inflammasomes. This article reviews the research progress of NLRP3 inflammasome activation and NEK7 kinase regulating the process of NLRP3 inflammasome activation, in order to provide new insights for the understanding of NLRP3 inflammasome activation by NEK7 on inflammatory activity in rheumatoid arthritis and future research related to targeted therapy.

Objective: To explore the prognostic significance of D-dimer, routine coagulation tests and platelet parameters in pregnant women. Methods: A total of 454 cases of pregnant women who came to Guangdong Tongjiang Hospital for examination and delivery from January 2020 to June 2021 were selected as the normal pregnancy group (96 cases in the first trimester, 138 cases in the second trimester, 114 cases in the third trimester, and 106 cases in the parturient period), another 60 cases of pregnancy with other diseases such as gestation combining with diabetes or hypertension were selected as the other-disease-combining group, and 45 cases of obstetric accidents were collected as the obstetric accident group. In addition, 167 cases of non-pregnant women in childbearing age who came to our hospital for health examination were selected as the healthy control group. The D-D level and coagulation tests including activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FIB), and thrombin time (TT) were compared among the four groups. The platelet parameters including platelet count (PLT), mean platelet volume (MPV), and platelet volume distribution width (PDW) were also compared among the four groups. Results: The levels of D-D and FIB in the normal pregnancy group were significantly lower than those in the other-disease-combining group and the obstetric accident group (P＜0.05). PT level in the normal pregnancy group was significantly higher than that in the other-disease-combining group and the obstetric accident group (P＜0.05). The PLT, MPV and PDW levels in the normal pregnancy group were significantly higher than those in the other-disease-combining group and the obstetric accident group (P＜0.05). Conclusion: Four indexes of blood coagulation, D-D and platelet parameters can effectively monitor the state of blood coagulation of pregnant women before delivery, which is of great significance in mastering the coagulation condition, improving the pregnancy outcome and preventing maternal thrombosis or postpartum hemorrhage, it is worthy of popularization and clinical promotion.

Objective: To explore the correlation between of senile depression based on the expression levels of nerve synaptic plasticity and leukoaraiosis (LA), serum neurogranin (NG), brain derived neurotrophin (BDNF). Methods: The elderly patients with depression admitted to our hospital from October 2020 to June 2021 were selected as the depression group (53 patients), and the normal physical examinees during the same period were selected as the non-depression group (145 patients). Univariate and multivariate Logistic regression were used to analyze the risk factors of senile depression, and Pearson correlation was used to analyze the relationship between serum NG, BDNF level, Fazekas score and HAMD score. Results: The expression level of serum NG in the depression group was higher than that in the non-depression group, and the expression level of serum BDNF was lower than that in the non-depression group (P＜0.05). Univariate analysis showed that age, Fazekas score, serum NG level and BDNF level were associated with senile depression. Multivariate Logistic regression analysis showed that age, serum levels of NG and BDNF were independent influencing factors of senile depression (P＜0.05). Pearson correlation analysis showed that serum NG level was positively correlated with HAMD score (r=0.751, P＜0.05), serum BDNF level was negatively correlated with HAMD score (r=-0.721, P＜0.05), Fazekas score was positively correlated with HAMD score (r=0.601, P＜0.001), and serum NG level was positively correlated with Fazekas score (r=0.640, P＜0.05). Conclusion: Abnormal expression of serum NG and BDNF may mediate the occurrence of senile depression based on synaptic plasticity ; and the more severe the degree of LA, the more prone to senile depression.

Objective: To explore the application effect of the Mobile Internet & Unified-Diabetes-Care model in the home management of diabetes.Methods: A total of 892 patients with type 2 diabetes who received medical treatment from March 2020 to October 2021 were selected for whole-process management by the Unified-Diabetes-Care team relying on the mobile terminal of the Internet blood glucose management platform.The questionnaire on cognition and behavior of diabetes-related knowledge and the self-management ability survey scale were used for collection and evaluation before and 6 and 12 months after management, respectively.At the same time, the changes of fasting blood glucose, blood glucose 2 hours after meals, glycosylated hemoglobin, blood lipid, urinary albumin/creatinine and other metabolic indexes before and after management were compared.Results: After 6 months and 12 months of management, the cognition and behavior of diabetes-related knowledge were improved compared with that before management, and increased with the extension of management time (P＜0.05).Diet control, regular exercise, medication compliance, blood glucose detection, complication management and scale total score were improved (P＜0.05), and the diet control, regular exercise and complication management were further improved after 12 months compared with 6 months of management (P＜0.05).The up-to-standard rates of blood glucose and blood pressure were significantly increased after management, and further increased with the passage of time (P＜0.05).Fasting blood glucose, 2 hours postprandial blood glucose, glycosylated hemoglobin, blood lipid, urinary albumin/creatinine were significantly decreased after management (P＜0.05), and fasting blood glucose, glycosylated hemoglobin, urinary albumin/creatinine were continuously improved after 12 months of management compared with 6 months of management (P＜0.05).Conclusion: Home management of type 2 diabetes patients through the Mobile Internet & Unified-Diabetes-Care model has continuously enhanced the self-management awareness and management behavior of patients, improved the metabolic indicators such as blood sugar, blood fat, urinary albumin/creatinine, and increased the standard compliance rate.The application of the new management model has greatly improved the level of chronic disease management.

Objective: To investigate the effects of bariatric surgery on food intake and body weight in obese rats by regulating Ghrelin. Methods: Ninety male 5-week-old SD rats were randomly divided into normal diet feeding group (n=15) and high fat diet feeding group (n=75). After 5 weeks of high fat diet feeding, the rats in the high fat diet group whose body weight was 20 % higher than that in the normal diet group were considered as successful obesity models. The rats in high-fat diet group were randomly divided into obesity model group, sham operation group and bariatric operation group, with 15 rats in each group. The body weight (once a week) and daily food intake of rats were recorded during the modeling period. The sleeve gastrectomy was performed in the bariatric operation group, and the changes of body mass and food intake in each group were recorded after 1-4 weeks. At the 4th week, blood samples from abdominal aorta were collected to detect the contents of triglyceride (TG) and total cholesterol (TC) in serum, and the protein expression of Ghrelin, mTOR, p-mTOR, p70s6k and p-p70s6k in gastric tissue was detected by WB. Results: Compared with the obesity model group and the sham operation group, the food intake and body weight of obese rats in the bariatric operation group were decreased (P＜0.05). The contents of serum triglyceride and total cholesterol in bariatric surgery group were higher than those in sham operation group and obesity model group at the 4th week after bariatric surgery (P＜0.05). Compared with the obesity model group, the protein expressions of p-mTOR, p-P70S6K and Ghrelin in the gastric tissue of the bariatric surgery group were significantly decreased (P＜0.05). Conclusion: Bariatric surgery may reduce food intake and body weight in obese rats by inhibiting mTOR/ P70S6K pathway and down-regulating the expression of Ghrelin that promotes food intake.

Objective: To investigate the value of circulating cell-free DNA (cfDNA) in the early diagnosis and disease activity of patients with rheumatoid arthritis (RA). To explore the relationship between cfDNA content and immunological diagnostic indicators of RA and related indicators of disease activity evaluation (rheumatoid factor (RF), Anti-cyclic citrullinated peptide antibodies (anti-CCP antibodies), C-reactive protein (CRP), Erythrocyte sedimentation rate (ESR)) and Disease Activity Score of 28 joints (DAS28-ESR). Methods: A total of 80 newly diagnosed RA patients who did not receive anti-rheumatic drugs were selected as the observation group (RA group), and 40 healthy people were selected as the control group (HC group). The serum cfDNA content of two groups was measured and the integrity was calculated. The correlation between cfDNA content and clinical indicators of RA patients was analyzed. Results: The RA group had a significantly higher level of cfDNA than the HC group (P＜0.05), but there was no correlation between and diagnostic serological markers (P＞0.05). According to the disease activity index of 28 joints (DAS28-ESR score), RA patients were stratified by disease activity, and it was found that the cfDNA content in the high DAS28-ESR score group was higher than that in the low DAS28-ESR score group (P＜0.05). cfDNA was correlated with DAS28-ESR, CRP and ESR (P＜0.05). Conclusion: The content of serum cfDNA in RA patients is increased, which is correlated with inflammatory response. It has diagnostic value for RA and may be an independent risk factor for the pathogenesis of RA.

Objective: To analyze the effect of the adjuvant therapy with bronchoalveolar lavage, pulmonary surfactant protein,lactate dehydrogenase (LDH), penetrating hormone 3 (PTX3) level, and arterial blood gas indexes in children with refractory mycoplasma pneumonia (RMPP). Methods: Clinical data of 97 children with RMPP in our hospital from October 2016 to June 2022 were collected retrospectively, and divided into the drug treatment group (48 cases) and bronchoalveolar lavage group (49 cases) according to the different treatment methods. The children in the drug treatment group were treated with macrolide for anti-infection and symptomatic treatment. The children in the bronchoalveolar lavage group were treated with bronchoscopic bronchoalveolar lavage on the basis of the drug treatment group. The curative effect, the levels of pulmonary surfactant protein A (SP-A), pulmonary surfactant protein D (SP-D), LDH and PTX3 and arterial blood gas indexes before and after treatment of the two groups were compared. Results: The total effective rate in the bronchoalveolar lavage group was 89.80 %, which was obviously higher than that in the drug treatment group (70.83 %) (P＜0.05). Before treatment, the levels of SP-A, SP-D, LDH, PTX3 and arterial blood gas indexes had no significant difference between the two groups (P＞0.05). After treatment, the levels of SP-A, SP-D, LDH and PTX3 in the bronchoalveolar lavage group were obviously lower than those in the drug treatment group, and the arterial blood gas indexes were obviously better than those in the drug treatment group (P＜0.05). The incidence of adverse reactions in the bronchoalveolar lavage group (10.00 %) was obviously lower than the drug treatment group (26.00 %) (P＜0.05). Conclusion: The adjuvant therapy with bronchoalveolar lavage in the treatment children with RMPP has a good treatment effect, which could significantly improve the arterial blood gas indexes and lower the levels of pulmonary surfactant protein (SP-A, SP-D), LDH and PTX3 with high safety.

Objective: To investigate the antidepressive effect of Qingxin Chenxiang Bawei Powder and its effect on monoamine neurotransmitters.Methods: Kunming male mice were randomly divided into the blank control group (distilled water), positive-drug group (fluoxetine hydrochloride 0.02 g/kg) and Mongolian medicine Qingxin Chenxiang Bawei Powder groups [the high-dose (27 g/kg) group, medium-dose (9 g/kg)group and low-dose (3 g/kg) group].Intragastric administration were performed on all experimental mice.Mice tail suspension test (TST) and forced swimming test (FST) were used to evaluate the antidepressive effect of the drug 7 days after intragastric administration.After FST behavioral experiment, the contents of 5-HT, DA and NE in plasma and hippocampus were determined by enzyme-linked immunosorbent assay (ELISA).Results: Compared with the blank control group, medium-dose and low-dose groups of Mongolian medicine could significantly shorten the immobility time in mice FST, and the difference was statistically significant (P＜0.05).The immobility time in mice TST was significantly shortened in the low-dose group, and the difference was statistically significant(P＜0.05).The plasma DA level was significantly increased in the low-dose group, and the difference was statistically significant (P＜0.05).The levels of 5-HT, DA and NE in the hippocampus of mice in the low-dose, medium-dose and high-dose groups were significantly increased, and the difference was statistically significant (P＜0.05).Conclusion: Qingxin Chenxiang Bawei Powder have certain antidepressive effect on mice, which may be related to its regulation effect on monoamine neurotransmitter content.