Objective: To investigate the expression of adrenomedullin (ADM) gene in liver cancer and its effect on the survival and prognosis. Methods: Kaplan-Meier Plotter database was used to analyze the effect of ADM gene expression on overall survival and progression-free survival of patients with liver cancer. Results: The ADM gene in BioGPS database was lowly expressed in human normal liver tissue (16.90±0.00). A total of 425 studies on ADM gene were retrieved from the Oncomine database, while there were 43 studies with statistically significant differential expression, of which 22 studies were highly expressed and 21 studies were lowly expressed. Kaplan-Meier Plotter database results showed that the overall survival (OS) and progression-free survival (PFS) of patients in the ADM high expression group were significantly shorter than those in the low expression group (P＜0.001). Conclusion: ADM is highly expressed in liver cancer tissue, and the ADM expression is related to the prognosis of liver cancer patients. It is expected to provide an important basis for the treatment and prognosis of liver cancer.

Objective: To systematically evaluate the influencing factors of depression and anxiety among empty nesters in China. Methods: The Chinese and English databases were searched from the inception to July 2022. The literature screening,quality assessment,and information extraction were performed by 2 researchers independently. RevMan 5.4 and Stata 15.0 were used for Meta-analysis. Results: A total of 22 studies with 34 911 participants were included. Among them,the related factors of depression were reported in 19 studies,and the related factors of anxiety in empty nesters were reported in 7 studies. The Meta-analysis suggested that the risk factors for depression among empty nesters were living without spouse [OR=1.39(1.21,1.59)],low frequency of children’s visits [OR=1.49(1.34,1.67)],living in rural areas[OR=1.40(1.22,1.62)],religious belief [OR=2.66(1.68,4.22)],poor self-rated health status [OR=2.16(1.80,2.58)],comorbid chronic diseases [OR=1.39(1.14,1.69)],physical pain [OR=2.09(1.62,2.70)],poor self-care ability in life [OR=2.01(1.22,3.32)],multiple somatic disorders [OR=1.98(1.71,2.29)],and poor economic status [OR=2.43(1.96,3.02)]. The protective factors for depression among empty-nesters were high education level [OR=0.80(0.71,0.90)],living no alone [OR=0.61(0.39,0.95)],physical exercise [OR=0.42(0.29,0.61)],drinking [OR=0.52(0.34,0.97)],and high social support [OR=0.96(0.94,0.98)]. Gender and age were not associated with depression among empty nesters(P＞0.05). In addition,the risk factors for anxiety among empty nesters were poor economic status [OR=1.80(1.49,2.19)],living without spouse [OR=1.88(1.35,2.64)],religious belief [OR=2.39(1.74,3.27)],and multiple somatic disorders [OR=2.81(2.05,3.85)]. No significant associations were found among chronic diseases and anxiety in empty nesters(P＞0.05). Conclusion: Numerous factors that contribute to depression and anxiety among empty nesters in China. Therefore,preventive measures should be timely taken to lower incidence of depression and anxiety in empty nesters of China.

Objective: To study the anti-nonalcoholic steatohepatitis mechanism of total flavonoids from Scabiosa comosa by network pharmacology. Methods: Based on the TCMSP database and literature reports, the main active components of total flavonoids from Scabiosa comosa were obtained. The Swiss Target Prediction server was used to predict the target of its active ingredients according to the reverse pharmacophore matching method, and the targets for improving NASH were screened out through databases such as Genecards, OMIM and Disgenet. The potential targets of drug targets and disease targets were used to make the Venn diagram. The Cytoscape software was used to construct the ' active ingredient-target ' network diagram. The STRING platform was used to construct the protein interaction network. The DAVID database and ClueGO plug-in were used for GO enrichment analysis and KEGG pathway analysis. Results: A total of 43 active components and 439 targets of total flavonoids were screened. The main active components in the network were ononin, camphene -3-O- rutinoside, isorhamnetin, quercetin, luteolin, eupatorium odoratum, etc. The key targets in the protein interaction network were TNF, AKT1, TP53, VEGFA, IL1B, etc. GO enrichment analysis involved 503 biological processes, 54 cellular components and 120 molecular functions. KEGG pathway enrichment analysis screened the top 20 related pathways, mainly acting on VEGF signaling pathway, PPAR signaling pathway, estrogen signaling pathway and so on. Conclusion: The flavonoid components from Scabiosa comosa can play a role in the treatment of nonalcoholic steatohepatitis through multiple targets and multiple pathways. The results provide a scientific basis for further study of the role of Scabiosa comosa in the field of anti-nonalcoholic steatohepatitis.

Objective: To investigate whether intestinal fecal microbiota transplantation (FMT) can improve the efficacy of anti-programmed cell death protein 1 (PD-1) therapy in the treatment of ovarian cancer and its possible mechanisms of action. Methods: Twenty 5-week-old C57bl/6 SPF female mice were randomly divided into four groups, with five mice in each group, namely the control group (NC group), fecal bacteria transplantation FMT group, PD-1 group, and the combined treatment group (FMT combined with PD-1 group). Each group of mice was uniformly treated with broad-spectrum antibiotics for 2 weeks to construct a pseudo sterile mouse model. On day 0, ID8-luc cells were injected intraperitoneally to construct an ovarian cancer bearing mouse model. Before medication and 2 weeks after medication treatment, mice were subjected to one in vivo imaging system (IVIS) to evaluate the therapeutic effect. After the end of the experiment on the 30th day, the mice were decapitated and killed. Peritoneal tumor tissue was taken for HE and immunohistochemical staining to observe the pathological changes of the tumor tissue. Results: The combined treatment group was better than the single PD-1 group in treating ovarian cancer bearing mice, and reduced the expression of PD-L1 in tumor cells (P＜0.05),and increased the infiltration of CD8⁺T cells in the tumor microenvironment (TME) (P＜0.05). Conclusion: Fecal microbiota transplantation (FMT) enhances the efficacy of PD-1 monoclonal antibody in treating ovarian cancer bearing mice by increasing the infiltration of CD8⁺T cells in the tumor microenvironment (TME).

Objective: To investigate the clinical value of CD26 and CLDN1 in the prevention and treatment of papillary thyroid carcinoma (PTC). Methods: Pathological tissue section specimens of 48 surgical patients with PTC admitted to Baotou Cancer Hospital from December 2021 to December 2022 were selected, with tumor tissues as the experimental group and adjacent tissues as the control group. The expression levels of CD26 and CLDN1 in the two groups were measured by immunohistochemistry. SPSS 27.0 was used to analyze the relationship between CD26, CLDN1 and clinicopathology in PTC patients. The correlation between the positive rates of each group was tested by Spearman correlation analysis (α＝0.05). Results: The positive expression rates of CD26 and CLDN1 in tumor tissues were 85.42% and 83.33%, respectively, which were higher than 27.08% and 20.83% in adjacent tissues, and the differences were statistically significant (P＜0.05). There was no significant difference in the positive expression rate of CD26 and CLDN1 protein in different PTC patients with different age, gender, maximum tumor diameter, unilateral, bilateral thyroid gland tumors, and invasion of the capsule (all P＞0.05). CD26 and CLDN1 were correlated with lymph node metastasis(P＜0.05). There was a significant positive correlation between the expression of CD26 and CLDN1 in PTC tissues (rs＝0.294, P＜0.05). Conclusion: CD26 and CLDN1 play an important role in the occurrence and development of PTC. Combined detection of CD26 and CLDN1 is helpful to the diagnosis and treatment of PTC.

Objective: To explore the dynamic changes of metabolic activity after multiple intakes of alcohol, and to analyze the induction and gender differences of metabolic enzyme activity. Methods: The end-expiratory alcohol detection method of rats was established by breath alcohol detectors, and the breath alcohol concentration of rats after intragastric administration of alcohol was detected. The time-varying changes of blood alcohol concentration of rats after alcohol intake were dynamically measured, and the pharmacokinetic parameters were calculated to evaluate cytochrome P450 2E1(CYP2E1)metabolic activity and gender differences in rats. Results: With the increase of alcohol intake, the metabolic activity of CYP2E1 showed an induced trend (P＜0.05). The activity of alcohol metabolism in female rats was stronger than that in male rats (P＜0.05). Conclusion: Alcohol metabolism and induction have sexual dimorphism.

Objective: To identify the related genes in patients with childhood asthma (CA) based on bioinformatics and immunoassay, and to explore its potential value in the diagnosis of CA. Methods: The GSE27011 data set was downloaded from the Gene Expression Omnibus (GEO) database, and the differentially expressed genes were screened by RStudio. The immune differences between the disease group and the normal group were analyzed respectively. The PPI network was drawn and the Hub genes were screened. The Hub genes were used to select the genes most related to immunity, and the prediction model was constructed by using the screened genes to make a nomogram. Finally, the independent GSE18965 data set was selected for verification. Results: A total of 37 differentially expressed genes were obtained. The difference analysis of immune cells showed that the levels of natural killer cells and plasma cell-like dendritic cells in CA were higher, and the difference of immune function showed that the cytotoxic activity of CA and the level of promoting inflammatory immune function were higher. Ten Hub genes were selected through the PPI network, and then five genes most related to immunity were screened, namely KLRF1, KLRD1, PRF1, IL2RB, and NCAM1. Using five genes to construct models and verify, it was found that KLRF1, KLRD1, PRF1 genes and CA had high predictive diagnostic value. Conclusion: The identified KLRF1, KLRD1 and PRF1 genes may be biomarkers for the diagnosis of CA, and natural killer cells may be a potential therapeutic target for CA.

白益母草为唇形科脓疮草属脓疮草的地上部分,具有解毒消肿等功效。白益母草中含有生物碱类、黄酮类、甾醇类等成分,也含有多种微量元素和有机酸。作为蒙药传统药材,白益母草被蒙医视为疮疾圣药。研究证实白益母草具有显著的抗炎功效,并且在治疗痛经,保护血管,预防高血压、糖尿病等方面具有良好的作用,临床应用前景广泛。本文对近年来白益母草化学成分和临床应用研究,特别是抗炎作用研究进展进行总结,为今后白益母草的深入研究和开发利用提供参考。

Objective: To construct a gene plasmid vector carrying NLRP3 and NLRP12 mutations in Wistar rats according to the NLRP3 (p.V72M, c.214G＞A) and NLRP12 (p.R754H, c.2261G＞A) gene mutation sites of the familial cold autoinflammatory syndrome (FCAS) family. Methods: According to the amino acid homology, the NLRP3 and NLRP12 gene mutation plasmids of rats were designed. The target genes were amplified by polymerase chain reaction (PCR) and recovered. The double enzyme digestion and connection were performed on the pCMV-mCherry-MCS-Neo vector. The connected products were transformed into competent cells. The positive transformants were identified by colony PCR, plasmid extraction electrophoresis and sequencing. Results: Agarose gel electrophoresis showed that the NLRP3 and NLRP12 mutant genes were successfully amplified. The gene mutation plasmid was confirmed by enzyme digestion electrophoresis and DNA sequencing. The gene sequence was completely correct and the recombinant plasmid vector was successfully constructed. Conclusion: The plasmid vectors of NLRP3 and NLRP12 gene mutations is successfully constructed, which provides a biological basis for further exploring the pathogenesis of FCAS caused by NLRP3 and NLRP12 gene mutations and the functional study of NLRP3 and NLRP12 genes.

Objective: To study and analyze the changes of intestinal flora in 1-5 years after cholecystectomy. Methods: A total of 15 fecal samples of healthy people and 16 fecal samples of people 1 to 5 years after cholecystectomy were collected from Baotou Central Hospital. The collected samples were sequenced to analyze the composition, richness and diversity of intestinal microbial flora in the two groups, and to compare the differences of intestinal microbial flora between the two groups. Results: (1) At the phylum level, the relative abundance of Bacteroidetes, Proteobacteria, Verrucomicrobia and Fusobacteria increased, while the relative abundance of Actinobacteria decreased in the population 1-5 years after cholecystectomy. (2) At the genus level, the relative abundance of Escherichia, Ruminococcus, Blautia, and Gemmatiaceae increased, while the relative abundance of Faecalibacterium, Bifidobacterium, Collins, and Agathobacter decreased in the population 1 to 5 years after cholecystectomy. The Chao1 index and Observed _ species index of the healthy population group were higher than those of the population group 1-5 years after cholecystectomy, and the differences were statistically significant (P＜0.05). The Pielou evenness of the healthy population group was slightly lower than that of the population group 1-5 years after cholecystectomy, and the difference was statistically significant (P＜0.05). Lachnospiraceae, Ruminococcus, Faecalimonas and Burkholderia were the intestinal flora with significant differences between the groups in the population group from 1 to 5 years after cholecystectomy. Conclusion: (1) The composition of intestinal flora in the population 1-5 years after cholecystectomy has changed. (2) The relative abundance of short-chain fatty acid-producing bacteria and probiotics in the population 1-5 years after cholecystectomy decreased, while the relative abundance of pathogenic bacteria increased.

血清前列腺特异性抗原(prostate-specific antigen, PSA)这一血清标志物目前已被广泛运用到前列腺癌(prostate cancer, PCa)的筛查和诊断,但对于血清总前列腺特异性抗原(total prostate-specific antigen 2, tPSA)处于4～10 ng/mL诊断灰区的前列腺癌,其诊断意义有限。为提高了前列腺癌患者PSA处于灰区时的诊断率,减少不必要的有创穿刺操作,本文就前列腺特异性抗原同源异构体2(prostate-specific antigen isoform 2, p2PSA)及前列腺健康指数(prostate health index, PHI)对于早期有临床意义前列腺癌(clinically significant prostate cancer, csPCa)诊断的临床应用价值,与前列腺癌的侵袭性,以及对于前列腺癌患者的疾病进展、预后、复发等方面都具有潜在的作用进行论述。近些年,多参数磁共振成像(mpMRI)也成为了预测csPCa的常用工具,使用MRI和PHI相互补充可用于检测早期有临床意义的前列腺癌。

Objective: To explore the current situation and influencing factors of college students' health emergency literacy. Methods: The three-stage cluster sampling were used for stratified cluster random sampling, and college students majoring in arts, science and medicine in three universities were surveyed with self-designed questionnaire on health emergency literacy. Chi-square test and multi-level Logistic regression model were used for statistical analysis. Results: The average score of health emergency literacy knowledge and skills among the selected college students was (56.68±6.50),52.63% of which having basic health emergency literacy scores ≥60.Among the five aspects in the questionnaire, scores on nuclear and radiation incidents had the highest pass rate (90.64%), while the average score and pass rate of general knowledge applicable to various incidents were lower. The pass rate of female college students was higher than that of male in health emergency literacy awareness, infectious disease, and emergency medical rescue (P＜0.05). In health emergency literacy awareness, infectious disease, emergency medical rescue, and group acute poisoning events, the scores was statistically different among different age groups (P＜0.05). There was no significant difference in health emergency literacy between college students with agricultural household registration and non-agricultural household registration (P＞0.05). Multivariate logistic regression analysis results showed that those of 19-21 years old(OR=3.069)or with average monthly household income of 1000-1999 Yuan(OR=8.834)had lower health emergency literacy, while college students from minority nationality(OR=0.150)had higher health emergency literacy. Conclusion: The health emergency literacy level of college students, especially the general knowledge applicable to various events, needs to be improved. Further measures should be taken to improve the health emergency literacy of college students.

Objective: To analyze the core drug pairs and their molecular biological mechanisms for the treatment of hepatitis B cirrhosis based on data mining and network pharmacology. Methods: Retrieve all the literatures related to the application of traditional Chinese medicine in the treatment of hepatitis B cirrhosis from January 2000 to December 2022 in China National Knowledge Infrastructure, Wanfang Database and VIP Science and Technology Journal, and study the medication rules of the prescriptions that meet the standards. The commonly used core drug pairs were subjected to pharmacological analysis, and the active components, main targets, and action pathways were predicted. Finally, molecular docking technology was used to simulate and verify on the computer. Results: A total of 275 prescriptions for the treatment of hepatitis B cirrhosis were screened. The analysis showed that TCM for the treatment of hepatitis B cirrhosis included 13 kinds of drugs such as Huangqi and Danshen. The core combination mode was 28 groups. Quercetin,Luteolin,Kaempferoland other ingredients were found as the main components of core prescription drugs for the treatment of hepatitis B cirrhosis,involving core targets such as serine/threonine protein kinase 1 (AKT1), tumor necrosis factor (TNF), tumor protein p53 (TP53), interleukin-6 (IL-6), vascular endothelial growth factor A (VEGFA), Caspase-3 (CASP3) and phosphatidylinositol-3-kinase/protein kinase (PI3K/Akt), TNF, interleukin-17 (Interleukin-17). Conclusion: The treatment principle of hepatitis B cirrhosis is to strengthen the body and relieve masses. "Huangqi-Danshen" can play a role in regulating cell growth, proliferation, survival and inflammatory reaction by interfering with PI3K-Akt, TNF, IL-17 and other signal pathways, so as to delay the progression of liver cirrhosis in hepatitis B.

Objective: To explore the current situation and influencing factors of long-term cognitive dysfunction in patients with valvular heart disease after operation, and to provide basis for postoperative nursing and rehabilitation guidance. Methods: A total of 248 patients with valvular heart disease who were admitted to the Department of Cardiothoracic Surgery of Yijishan Hospital from May 2022 to May 2023 were selected as the research objects. The T-MoCA scale was used to evaluate the patients at the third month after operation to determine whether the patients had postoperative cognitive dysfunction (POCD). Results: The incidence of POCD in patients with valvular heart disease was 17.8% at 3 months after operation. The results of univariate analysis showed that age, education level, combined cerebrovascular disease, BMI, preoperative T-MoCA score, anesthesia time, cardiopulmonary bypass time, mechanical ventilation time, postoperative hospitalization days, total hospitalization days and postoperative ICU hospitalization time were the influencing factors of POCD in the third month after operation, and the difference was statistically significant (P＜0.05). Multivariate Logistic regression analysis showed that advanced age, long cardiopulmonary bypass time, high BMI, long postoperative ICU hospitalization time, and cerebrovascular disease were independent risk factors for POCD in the third month after surgery (P＜0.05). Conclusion: The incidence of cognitive dysfunction in elderly patients with valvular heart disease is higher in the third month after operation. Patients with advanced age, cerebrovascular disease, high BMI, low preoperative T-MOCA score, anesthesia time, cardiopulmonary bypass time, mechanical ventilation time, postoperative hospitalization days, total hospitalization days, and long postoperative ICU hospitalization time are at higher risk. Therefore, effective measures and means should be taken to reduce the incidence of POCD, improve the prognosis of patients, and shorten the postoperative rehabilitation treatment and nursing time.

Objective: To investigate the relationship between the activity and gene polymorphism of coagulation factor Ⅻ and the risk of recurrent spontaneous abortion (RSA) in Baotou Han population. Methods: RSA patients and healthy pregnant women were selected as the research objects. The activity of coagulation factor Ⅻ was measured. The genotype composition of FⅫ gene single nucleotide polymorphism (SNP) rs1801020 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results of the two groups were analyzed to determine the correlation between FⅫ gene SNP rs1801020 and RSA under different genetic models. Results: There were significant differences in the genotype and allele frequency distribution of SNP rs1801020 in FⅫ gene between the two groups (P＜0.05); the frequency of T allele in RSA group was lower than that in control group (χ²=8.756, OR=2.043, 95%CI: 1.267-3.295,P<0.05); the frequency of CT genotype in RSA group was higher than that in control group, while the frequency of TT genotype was lower than that in control group, and the difference was statistically significant (χ²=9.905, OR=2.611, 95%CI: 1.447-4.713,P＜0.05); FⅫ activity in RSA patients was different among rs1801020 genotypes, and the order of activity was CC, CT and TT. FⅫ SNP rs1801020 was associated with the risk of RSA under codominant, dominant and overdominant models (OR=2.611, 2.636, 2.470, P＜0.05); compared with TT genotype, CT and CT+CC genotypes could increase the risk of RSA; compared with CC+TT genotype, CT genotype could increase the risk of RSA. Conclusion: The difference in the distribution of FⅫ rs1801020 between the two groups may be related to the risk of RSA in Baotou Han women. Compared with TT genotype, mutant heterozygous CT genotype may increase the risk of RSA, the T allele of rs1801020 locus of FⅫ gene can cause the decrease of FⅫ activity.

Objective: To investigate the efficacy of desonide cream combined with Heling emollient in the treatment of mild to moderate atopic dermatitis in infants. Methods: A total of 60 infants with atopic dermatitis treated in our hospital from June 2022 to June 2023 were randomly divided into the control group (n=30) and the observation group(n=30). The control group was treated with desonide cream alone, the observation group was treated with desonide cream combined with Heling emollient. Scoring atopic dermatitis (SCORAD) before and after treatment and the clinical efficacy were compared between the two groups of patients. Results: After 2 weeks of treatment, the SCORAD scores and clinical efficacy of two groups were both improved, and the SCORAD score and effective rate in the observation group were better than those in the control group (P＜0.05). The incidence rate of adverse reactions in the observation group and the control group was 3.33% and 16.66% respectively (P＞0.05). Conclusion: Desonide cream combined with Heling emollient can significantly reduce clinical symptoms and improve the therapeutic effect in infants with mild to moderate atopic dermatitis.

Objective: To detect differentially expressed miRNAs in exosomes derived from intestinal epithelial cells of essential hypertensive rats, and to enrich and analyze their target genes. Methods: Three Spontaneously hypertensive rats (SHR) and three Wistar-Kyoto rats (WKY) were selected as the research objects. The expression of miRNA in exosomes derived from intestinal epithelial cells of rats was detected by high-throughput sequencing. The differentially expressed miRNAs in the two groups of rats were screened and their target genes were predicted. The target genes were analyzed by Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) using biological function software. Results: Compared with the WKY group, there were 37 differentially expressed miRNAs in the SHR group (All P＜0.05), 32 miRNAs were up-regulated and 5 miRNAs were down-regulated. Among them, rno-miR-208b-3p, rno-miR-134-5p, rno-miR-93-3p, rno-miR-378b, rno-miR-96-5p, rno-miR-210-3p, and rno-miR-208a-3p were noteworthy. A total of 10 662 miRNA downstream target genes were predicted, and a total of 34 127 target sites were predicted. GO enrichment was mainly concentrated in membrane-bound organelles, protein binding, and positively regulated biological processes. KEGG enrichment was mainly concentrated in MAPK, mTOR, Ras and TNF signaling pathways. Conclusion: The expression of miRNA in exosomes derived from intestinal epithelial cells of essential hypertensive rats was significantly different. Its target genes may be involved in MAPK, mTOR, Ras and TNF signaling pathways through cell growth and differentiation, angiogenesis and metabolic function, thus affecting the occurrence and development of hypertensive diseases.

Objective: To compare the effects of closed reduction under non-anesthesia and axillary brachial plexus block on patients with distal radius fractures. Methods: From May 2021 to May 2023, 88 patients aged 0~16 years old who underwent distal radius fracture treatment in Beijing Jishuitan Hospital Guizhou Hospital were selected and randomly divided into control group (44 patients) and experimental group (44 patients). The control group underwent closed reduction under non-anesthesia, the experimental group underwent closed reduction under axillary brachial plexus block. Mean artery pressure (MAP), mean heart rate and mean respiration were measured. The wrist joint activity before and after reduction was recorded. Visual Analogue Scale (VAS) was used to evaluate the degree of patients' own pain. The success rate of reduction and the incidence of complications in the two groups were recorded. Results: After reduction, the MAP, mean heart rate and mean respiration level in the experimental group were lower than those in the control group (P＜0.05). The levels of radial bias angle, palm curvature, pronation and supination in the experimental group were higher than those in the control group (P＜0.05). The VAS score level at the immediate, 12 h, 24 h and 48 h postoperative period in the experimental group was lower than that in the control group (P＜0.05). The reduction success rate in the experimental group was higher than that in the control group (P＜0.05). The overall complication rate was slightly higher in the experimental group than that in the control group (P＞0.05). Conclusion: Compared with closed reduction under non-anesthetic state, closed reduction under axillary brachial plexus block can reduce blood pressure, heart rate, respiration and pain in children with distal radius fracture, improve their wrist function and success rate of reduction, with high safety.

Objective: To explore the genes and mechanisms in colorectal cancer and adjacent tissues at different distances by bioinformatics analysis. Methods: Eukaryotic mRNA was sequenced by Illumina Novaseq 6000 sequencing platform. Results: Differential expression analysis showed that there were 28 000 differentially expressed genes in the upper margin group of colorectal cancer, of which 11 735 were up-regulated and 16 265 were down-regulated. There were 32 438 differentially expressed genes in the lower margin group of colorectal cancer, of which 11 477 were up-regulated and 20 961 were down-regulated. The differentially expressed genes IGF2BP1, ADAM12, CDON, AKAP6, IGSF9B, LONR2 and KCNB1 were found to be down-regulated in colorectal cancer tissues and adjacent tissues at different distances. Functional annotation analysis of differential genes showed that differential genes were mainly involved in cellular processes, and the pathways involved were mainly metabolism, signal transduction and immune system. In terms of pathogenesis, it mainly exists in cancer, nervous system diseases and gastrointestinal system diseases. Differential gene enrichment analysis showed that differential genes were mainly in cardiac conduction, collagen fiber tissue, collagen decomposition process, etc., and the enriched pathways were mainly human papillomavirus infection pathway and P13K-Akt signaling pathway. Conclusion: The expression of differential genes IGF2BP1, ADAM12, CDON, AKAP6, IGSF9 B, LONR2 and KCNB1 is down-regulated in colorectal cancer tissues and adjacent tissues at different distances. Differential genes exist in cells and are mainly involved in metabolic pathways, signal transduction pathways, and immune system pathways. The pathogenesis mainly exists in cancer, nervous system diseases and gastrointestinal system diseases.

Objective: To investigate the prevalence of metabolic syndrome (MS) and Helicobacter pylori (Hp) infection, and to analyze the relationship between MS and Hp infection, uric acid (UA). Methods: A total of 305 patients with HP infection and UA were selected from the Zhangzhou Affiliated Hospital of Fujian Medical University from January 2022 to September 2022, they were divided into the MS Group and non-MS Group, HP (+) group and HP (-) group. BP and blood glucose were compared between groups. Binary logistic regression model was used to analyze the risk factors of MS and the relationship between Hp infection and age and sex. Results: The incidence of MS and Hp infection was 15.41% and 44.92% respectively. There were significant differences in age, male ratio, Hp infection rate, UA, BMI, TG, blood glucose, BP and HDL-C between the MS Group and the non-MS Group (P＜0.05). There were significant differences in age, MS ratio, UA, BMI, overweight, TG, hypertriglyceridemia, BP, hypertension, HDL-C and low HDL-C between Hp (+) group and Hp (-) group (P＜0.05). Age was an independent risk factor for Hp infection. Hp infection, male gender, HDL-C, blood glucose, DBP and BMI were independent risk factors for MS. Conclusion: The incidence of MS in male is higher than that in female. The relationship between Hp infection and sex is not obvious, but the infection rate increases with higher age. Hp infection and male are independent risk factors for MS, while UA was not.