Objective: To investigate the expression of adrenomedullin (ADM) gene in liver cancer and its effect on the survival and prognosis. Methods: Kaplan-Meier Plotter database was used to analyze the effect of ADM gene expression on overall survival and progression-free survival of patients with liver cancer. Results: The ADM gene in BioGPS database was lowly expressed in human normal liver tissue (16.90±0.00). A total of 425 studies on ADM gene were retrieved from the Oncomine database, while there were 43 studies with statistically significant differential expression, of which 22 studies were highly expressed and 21 studies were lowly expressed. Kaplan-Meier Plotter database results showed that the overall survival (OS) and progression-free survival (PFS) of patients in the ADM high expression group were significantly shorter than those in the low expression group (P＜0.001). Conclusion: ADM is highly expressed in liver cancer tissue, and the ADM expression is related to the prognosis of liver cancer patients. It is expected to provide an important basis for the treatment and prognosis of liver cancer.

Objective: To systematically evaluate the influencing factors of depression and anxiety among empty nesters in China. Methods: The Chinese and English databases were searched from the inception to July 2022. The literature screening,quality assessment,and information extraction were performed by 2 researchers independently. RevMan 5.4 and Stata 15.0 were used for Meta-analysis. Results: A total of 22 studies with 34 911 participants were included. Among them,the related factors of depression were reported in 19 studies,and the related factors of anxiety in empty nesters were reported in 7 studies. The Meta-analysis suggested that the risk factors for depression among empty nesters were living without spouse [OR=1.39(1.21,1.59)],low frequency of children’s visits [OR=1.49(1.34,1.67)],living in rural areas[OR=1.40(1.22,1.62)],religious belief [OR=2.66(1.68,4.22)],poor self-rated health status [OR=2.16(1.80,2.58)],comorbid chronic diseases [OR=1.39(1.14,1.69)],physical pain [OR=2.09(1.62,2.70)],poor self-care ability in life [OR=2.01(1.22,3.32)],multiple somatic disorders [OR=1.98(1.71,2.29)],and poor economic status [OR=2.43(1.96,3.02)]. The protective factors for depression among empty-nesters were high education level [OR=0.80(0.71,0.90)],living no alone [OR=0.61(0.39,0.95)],physical exercise [OR=0.42(0.29,0.61)],drinking [OR=0.52(0.34,0.97)],and high social support [OR=0.96(0.94,0.98)]. Gender and age were not associated with depression among empty nesters(P＞0.05). In addition,the risk factors for anxiety among empty nesters were poor economic status [OR=1.80(1.49,2.19)],living without spouse [OR=1.88(1.35,2.64)],religious belief [OR=2.39(1.74,3.27)],and multiple somatic disorders [OR=2.81(2.05,3.85)]. No significant associations were found among chronic diseases and anxiety in empty nesters(P＞0.05). Conclusion: Numerous factors that contribute to depression and anxiety among empty nesters in China. Therefore,preventive measures should be timely taken to lower incidence of depression and anxiety in empty nesters of China.

Objective: To explore the role of lipoyltransferase (LIPT1) in the development and immune infiltration of breast cancer by bioinformatics. Methods: TCGA, GEPIA, HPA, UALCAN, Kaplan-Meier, TIMER 2.0 and other databases were used to explore the differential expression, function and related mechanism of LIPT1 in breast cancer, and to analyze the effect of LIPT1 on the immune infiltration of invasive breast cancer. Results: The expression of LIPT1 was associated with invasive breast cancer, and PPI network analysis showed that LIPT1 was associated with ACSM1, DBT, DLAT, DLT, DLST, GCSH, LIAS, LIPT2, PDHB, and PDHX; the expression of LIPT1 was associated with immune cell infiltration in invasive breast cancer. Conclusion: Bioinformatics predicts that LIPT1 expression is down-regulated in invasive breast cancer and is associated with tumor immune cell infiltration.

Objective:To study the prokaryotic expression and purification of GroEL1 from mycobacterium tuberculosis by genetic engineering and bioinformatics, and to predict its structure and function, and to analyze its application value in novel tuberculosis vaccine. Methods:The GroEL1 gene was amplified by PCR in vitro and cloned into the pET28a plasmid. The pET28a-GroEL1 vector was successfully constructed by sequencing and transformed into the expression strain of E. coli BL21 (DE3). The recombinant GroEL1 protein was expressed by IPTG and purified by nickel affinity column. The nucleotide and amino acid sequences of GroEL1 of H37Ra strain were obtained from UniProt database. Protparam, TMHMM-2.0, Protscale, NetChop-3.1, Psortb and SignalP-4.1 were used to predict the physicochemical properties, transmembrane helix, hydrophilic/hydrophobic, phosphorylation sites, subcellular localization and signal peptide of GroEL1 protein, respectively. NetNGlyc-1.0 and YinOYang-1.2 predicted the glycosylation sites. SOPMA and Swissmodel predicted the secondary structure and tertiary structure of the protein. Clustalw compares homologous sequences. String predicted interacting proteins, IEBD and ABCpred predicted B-cell epitopes of proteins. Results:The recombinant pET28a-GroEL1 vector was successfully constructed and the GroEL1 protein partially expressed in soluble form in E. coli. The recombinant GroEL1 protein was purified by nickel affinity chromatography with a purity of more than 90%. Western blot analysis confirmed that the recombinant GroEL1 protein had good immunoreactivity. The GroEL1 gene of mycobacterium tuberculosis strain H37Ra has a total length of 1 620 bp, encoding 539 amino acids with a molecular weight of 55.88 kD and an isoelectric point of 4.98. It is predicted that the protein has strong hydrophilicity, stable properties, no transmembrane region, 37 possible phosphorylation modification sites and 8 O-glycosylation sites, which is a non-secreted protein. Located in the cytoplasm, the secondary structure showed α-helix (53.43%), extended chain (11.87%), β-turn(7.61%), random coil(27.09%), multiple B cell antigen epitopes and multiple GroEL1 protein interacting proteins. Conclusion:The recombinant GroEL1 protein was successfully expressed and purified, and the structure and function of GroEL1 protein were predicted by bioinformatics, which laid a foundation for the prevention, diagnosis and treatment of tuberculosis.

Objective:To systematically evaluate the risk factors of hyperuricemia (HUA) in middle-aged and elderly people in China. Methods:The Embase,Web of Science,PubMed,the Cochrane Library,Chinese National Knowledge Infrastructure (CNKI),Wangfang Data,Chinese Biomedical Literature Database (CBM),and VIP database were selected to collect literaturse on risk factors of HUA in middle-aged and elderly people in China. The retrieval time limitation was defined from the establishment of the database until March 2022. RevMan 5.3 and Stata 14.0 software were used for data analysis. Results:A total of 20 studies were included,including 72 020 cases of HUA patient. Meta-analysis results showed that hypertension (OR=1.43,95%CI: 1.26-1.62),age ≥45 (OR=1.11,95%CI: 1.07-1.15),alcohol consumption (OR=1.47,95%CI: 1.31-1.65),high body mass index (BMI) (OR=1.06,95%CI: 1.02-1.19),high triglyceride (TG) (OR=1.81,95%CI: 1.58-2.07),renal insufficiency (OR=2.88,95%CI: 1.76~4.73),high cholesterol (TC) (OR=1.21,95%CI: 1.09~1.33),high serum creatinine (OR=1.69,95%CI: 1.53~1.85),central obesity (OR=1.52,95%CI: 1.30-1.79) and high urea nitrogen (OR=1.53,95%CI: 1.23-1.90) were risk factors for HUA in middle-aged and elderly people in China. High density lipoprotein (HDL-C) (OR=0.50,95%CI:0.36~0.70) and hyperglycemia (OR=0.75,95%CI: 0.66-0.86) were protective factors to HUA in middle-aged and elderly people in China. Conclusion:Hypertension,age ≥45 years,alcohol consumption,high BMI,high TG,renal insufficiency,high TC,high serum creatinine,central obesity and high urea nitrogen are the risk factors for HUA in middle-aged and elderly people in China. Meanwhile,high blood glucose and HDL-C are the protective factors to HUA.

Objective: To study the anti-nonalcoholic steatohepatitis mechanism of total flavonoids from Scabiosa comosa by network pharmacology. Methods: Based on the TCMSP database and literature reports, the main active components of total flavonoids from Scabiosa comosa were obtained. The Swiss Target Prediction server was used to predict the target of its active ingredients according to the reverse pharmacophore matching method, and the targets for improving NASH were screened out through databases such as Genecards, OMIM and Disgenet. The potential targets of drug targets and disease targets were used to make the Venn diagram. The Cytoscape software was used to construct the ' active ingredient-target ' network diagram. The STRING platform was used to construct the protein interaction network. The DAVID database and ClueGO plug-in were used for GO enrichment analysis and KEGG pathway analysis. Results: A total of 43 active components and 439 targets of total flavonoids were screened. The main active components in the network were ononin, camphene -3-O- rutinoside, isorhamnetin, quercetin, luteolin, eupatorium odoratum, etc. The key targets in the protein interaction network were TNF, AKT1, TP53, VEGFA, IL1B, etc. GO enrichment analysis involved 503 biological processes, 54 cellular components and 120 molecular functions. KEGG pathway enrichment analysis screened the top 20 related pathways, mainly acting on VEGF signaling pathway, PPAR signaling pathway, estrogen signaling pathway and so on. Conclusion: The flavonoid components from Scabiosa comosa can play a role in the treatment of nonalcoholic steatohepatitis through multiple targets and multiple pathways. The results provide a scientific basis for further study of the role of Scabiosa comosa in the field of anti-nonalcoholic steatohepatitis.

Objective:To explore the key prognostic genes in the tumor microenvironment of endometrial carcinoma by bioinformatics analysis and PCR expression verification. Methods: The gene expression data of 552 patients with endometrial carcinoma were downloaded from TCGA database, and the immune score and interstitial score were performed by R software ESTIMATE algorithm to screen the differentially expressed genes. A variety of algorithms were used to further explore the prognosis of differential genes and related pathways. PCR assay was used to analyze the expression correlation of differential genes in clinical tissues and cell lines. Results: Cox regression analysis and KM survival analysis indicated that CCR4 was a potential prognostic gene. GSEA and KEGG results showed that CCR4 was significantly enriched in the immune response-activation cell surface receptor signaling pathway. The results of RT-qPCR showed that the expression of CCR4 in tumor group was significantly higher than that in non-tumor group at cell level and tissue level. Conclusion: Immunoinfiltration exists in the tumor microenvironment of endometrial carcinoma, and CCR4 is expected to be a new diagnostic marker for endometrial carcinoma.

Objective: To explore the dynamic changes of metabolic activity after multiple intakes of alcohol, and to analyze the induction and gender differences of metabolic enzyme activity. Methods: The end-expiratory alcohol detection method of rats was established by breath alcohol detectors, and the breath alcohol concentration of rats after intragastric administration of alcohol was detected. The time-varying changes of blood alcohol concentration of rats after alcohol intake were dynamically measured, and the pharmacokinetic parameters were calculated to evaluate cytochrome P450 2E1(CYP2E1)metabolic activity and gender differences in rats. Results: With the increase of alcohol intake, the metabolic activity of CYP2E1 showed an induced trend (P＜0.05). The activity of alcohol metabolism in female rats was stronger than that in male rats (P＜0.05). Conclusion: Alcohol metabolism and induction have sexual dimorphism.

Objective:To compare the effectiveness of fluorescence staining method and KOH wet mount method on detecting superficial fungal diseases and the detection rates at different detection sites. Methods: Specimens of patients who were diagnosed with superficial fungal disease at the Dermatology Department of the Second Affiliated Hospital of Baotou Medical College from October 2021 to March 2023 were collected. The fluorescence staining and KOH wet mount method were used for microscopic examination. The positive detection rate and the positive detection rate at different detection sites were compared. Results:The detection patients of the KOH wet mount method and fluorescence staining method were 558 and 691 cases (the positive rates were 38.35% and 58.47%) respectively, with a statistically significant difference in positive detection rates (χ²=49.967, P＜0.01). And the positive detection rate of fluorescence staining at the head, face, hands, feet, trunk, groin, and nail parts was higher than that of KOH wet mount method, and the differences were statistically significant. The differences were sequentially decreased from foot, head, face, groin, hand, nail, and trunk parts. Conclusion: The positive detection rate of fluorescence staining tests on superficial fungal diseases is significantly better than that of KOH wet mount method, which could reduce the missed and misdiagnosed rate. It is preferred to detect superficial fungal diseases at nail, foot, groin, head and face, while hand and body detection could choose KOH test or fluorescence staining test based on the clinical experience of doctors to relieve the economic burden of patients.

Objective: To investigate whether intestinal fecal microbiota transplantation (FMT) can improve the efficacy of anti-programmed cell death protein 1 (PD-1) therapy in the treatment of ovarian cancer and its possible mechanisms of action. Methods: Twenty 5-week-old C57bl/6 SPF female mice were randomly divided into four groups, with five mice in each group, namely the control group (NC group), fecal bacteria transplantation FMT group, PD-1 group, and the combined treatment group (FMT combined with PD-1 group). Each group of mice was uniformly treated with broad-spectrum antibiotics for 2 weeks to construct a pseudo sterile mouse model. On day 0, ID8-luc cells were injected intraperitoneally to construct an ovarian cancer bearing mouse model. Before medication and 2 weeks after medication treatment, mice were subjected to one in vivo imaging system (IVIS) to evaluate the therapeutic effect. After the end of the experiment on the 30th day, the mice were decapitated and killed. Peritoneal tumor tissue was taken for HE and immunohistochemical staining to observe the pathological changes of the tumor tissue. Results: The combined treatment group was better than the single PD-1 group in treating ovarian cancer bearing mice, and reduced the expression of PD-L1 in tumor cells (P＜0.05),and increased the infiltration of CD8⁺T cells in the tumor microenvironment (TME) (P＜0.05). Conclusion: Fecal microbiota transplantation (FMT) enhances the efficacy of PD-1 monoclonal antibody in treating ovarian cancer bearing mice by increasing the infiltration of CD8⁺T cells in the tumor microenvironment (TME).

糖尿病脑病(diabetic encephalopathy,DE)是糖尿病常见的严重慢性并发症,可导致认知功能障碍和大脑神经结构改变,其发病机制可能与神经炎症、氧化应激、胰岛素抵抗等有关。目前,关于糖尿病脑病及认知功能障碍的治疗仍处于探索阶段。本文旨在对糖尿病脑病认知功能障碍的影像学表现、发病机制、治疗等方面进行综述。

Objective: To investigate the clinical value of CD26 and CLDN1 in the prevention and treatment of papillary thyroid carcinoma (PTC). Methods: Pathological tissue section specimens of 48 surgical patients with PTC admitted to Baotou Cancer Hospital from December 2021 to December 2022 were selected, with tumor tissues as the experimental group and adjacent tissues as the control group. The expression levels of CD26 and CLDN1 in the two groups were measured by immunohistochemistry. SPSS 27.0 was used to analyze the relationship between CD26, CLDN1 and clinicopathology in PTC patients. The correlation between the positive rates of each group was tested by Spearman correlation analysis (α＝0.05). Results: The positive expression rates of CD26 and CLDN1 in tumor tissues were 85.42% and 83.33%, respectively, which were higher than 27.08% and 20.83% in adjacent tissues, and the differences were statistically significant (P＜0.05). There was no significant difference in the positive expression rate of CD26 and CLDN1 protein in different PTC patients with different age, gender, maximum tumor diameter, unilateral, bilateral thyroid gland tumors, and invasion of the capsule (all P＞0.05). CD26 and CLDN1 were correlated with lymph node metastasis(P＜0.05). There was a significant positive correlation between the expression of CD26 and CLDN1 in PTC tissues (rs＝0.294, P＜0.05). Conclusion: CD26 and CLDN1 play an important role in the occurrence and development of PTC. Combined detection of CD26 and CLDN1 is helpful to the diagnosis and treatment of PTC.

Objective: To analyze the clinical features, in-hospital treatment and 12-month outcomes of heart failure inpatients with different age shock indexes (ASI). Methods: A total of 259 heart failure patients aged over 18 years who were admitted in the Second Affiliated Hospital of Baotou Medical College from October 2020 to November 2021 were consecutively included in this study. The baseline data were collected and ASI (age × heart rate/systolic blood pressure) was calculated on the day of admission. Patients were divided into the high ASI (n=129) and low ASI group (n=129). Clinical features of the two groups of patients, differences in hospital treatment and outcomes of 12 months after discharge, and correlation between ASI and the risk for all-cause mortality 12 months after discharge in patients were analyzed. Results: Lower body mass index, systolic blood pressure, diastolic blood pressure and left ventricular ejection fraction value were found in the High ASI group of patients, with thinner left ventricular posterior wall and higher brain natriuretic peptide and white blood cell count. More patients in the high ASI group took β-blockers orally and used inotropes and diuretics intravenously than in the low ASI group (P＜0.05). 12 months after discharge, patients in the high ASI group had more rehospitalizations (34.9%) and all-cause deaths (23.3%), and a higher risk of all-cause death after 12 months. (HR=3.05, 95%CI: 1.25~7.45, P=0.014). Conclusion: Patients in the high ASI and low ASI groups had different clinical characteristics and similar treatments during hospitalization. The risk of all-cause death 12 months after discharge was higher in patients with high ASI than patients with low ASI. ASI was an independent risk factor for long-term prognosis of hospitalized patients with heart failure.

Objective:To provide evidence-based medical basis for the treatment of stress urinary incontinence (SUI) by warm acupuncture combining with pelvic floor muscle training using Meta-analysis. Methods: The subject words “warm acupuncture”, “urinary incontinence” and “stress urinary incontinence” were searched in PubMed, Cochrane Library Central Database, Embase, Chinese Biomedical Literature Database (CBM), China Knowledge Network Database (CNKI), Wanfang Data Knowledge Service Platform (Wanfang Data), and VIP Journal Full-text Database (VIP). The retrieval time limit was from the establishment of the database to June 2022. According to the requirements of Cochrane system evaluation, the methodological quality of the selected clinical trials was evaluated, the data were extracted and analyzed. Results: A total of 11 studies and 774 patients were included. Compared with the control group (conventional pelvic floor muscle training), Meta-analysis results showed that the total effective rate of warm acupuncture combined with pelvic floor muscle training group in the treatment of SUI was higher than that of the control group [OR= 4.02, 95%CI(2.58,6.26),P＜0.000 01], with the ICI-Q-SF score of [MD=-1.17, 95%CI(-1.63, -0.7),P＜ 0.000 01] and 1 h pad test of [MD=-1.65, 95%CI(-2.07, -1.23),P＜0.000 01], and the difference was statistically significant. No adverse reactions were reported in the included literature. Conclusion: Warm acupuncture combining with pelvic floor muscle training in the treatment of SUI can improve the quality of life of patients and reduce leakage of urine, which has a good effect.

Objective: To investigate the relationship between the activity and gene polymorphism of coagulation factor Ⅻ and the risk of recurrent spontaneous abortion (RSA) in Baotou Han population. Methods: RSA patients and healthy pregnant women were selected as the research objects. The activity of coagulation factor Ⅻ was measured. The genotype composition of FⅫ gene single nucleotide polymorphism (SNP) rs1801020 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results of the two groups were analyzed to determine the correlation between FⅫ gene SNP rs1801020 and RSA under different genetic models. Results: There were significant differences in the genotype and allele frequency distribution of SNP rs1801020 in FⅫ gene between the two groups (P＜0.05); the frequency of T allele in RSA group was lower than that in control group (χ²=8.756, OR=2.043, 95%CI: 1.267-3.295,P<0.05); the frequency of CT genotype in RSA group was higher than that in control group, while the frequency of TT genotype was lower than that in control group, and the difference was statistically significant (χ²=9.905, OR=2.611, 95%CI: 1.447-4.713,P＜0.05); FⅫ activity in RSA patients was different among rs1801020 genotypes, and the order of activity was CC, CT and TT. FⅫ SNP rs1801020 was associated with the risk of RSA under codominant, dominant and overdominant models (OR=2.611, 2.636, 2.470, P＜0.05); compared with TT genotype, CT and CT+CC genotypes could increase the risk of RSA; compared with CC+TT genotype, CT genotype could increase the risk of RSA. Conclusion: The difference in the distribution of FⅫ rs1801020 between the two groups may be related to the risk of RSA in Baotou Han women. Compared with TT genotype, mutant heterozygous CT genotype may increase the risk of RSA, the T allele of rs1801020 locus of FⅫ gene can cause the decrease of FⅫ activity.

电压依赖性阴离子通道(voltage dependent-anion channel, VDAC)位于线粒体外膜,是一种转运三磷酸腺苷 (adenosine triphosphate, ATP)和细胞间代谢产物的通道蛋白,对线粒体代谢及细胞生长起着重要的调节作用。最近的研究表明,VDAC在心肌病、动脉粥样硬化、高血压和糖尿病(diabetes mellitus, DM)中均有显著升高。糖尿病肾脏疾病(diabetes kidney disease, DKD)是糖尿病的主要微血管并发症,是导致终末期肾病的最常见原因,其发病机制复杂,VDAC参与其中。本文对VDAC蛋白的结构、特性、作用机制以及与DKD关系的研究进展进行综述。

Objective: To explore the current situation and influencing factors of college students' health emergency literacy. Methods: The three-stage cluster sampling were used for stratified cluster random sampling, and college students majoring in arts, science and medicine in three universities were surveyed with self-designed questionnaire on health emergency literacy. Chi-square test and multi-level Logistic regression model were used for statistical analysis. Results: The average score of health emergency literacy knowledge and skills among the selected college students was (56.68±6.50),52.63% of which having basic health emergency literacy scores ≥60.Among the five aspects in the questionnaire, scores on nuclear and radiation incidents had the highest pass rate (90.64%), while the average score and pass rate of general knowledge applicable to various incidents were lower. The pass rate of female college students was higher than that of male in health emergency literacy awareness, infectious disease, and emergency medical rescue (P＜0.05). In health emergency literacy awareness, infectious disease, emergency medical rescue, and group acute poisoning events, the scores was statistically different among different age groups (P＜0.05). There was no significant difference in health emergency literacy between college students with agricultural household registration and non-agricultural household registration (P＞0.05). Multivariate logistic regression analysis results showed that those of 19-21 years old(OR=3.069)or with average monthly household income of 1000-1999 Yuan(OR=8.834)had lower health emergency literacy, while college students from minority nationality(OR=0.150)had higher health emergency literacy. Conclusion: The health emergency literacy level of college students, especially the general knowledge applicable to various events, needs to be improved. Further measures should be taken to improve the health emergency literacy of college students.

血清前列腺特异性抗原(prostate-specific antigen, PSA)这一血清标志物目前已被广泛运用到前列腺癌(prostate cancer, PCa)的筛查和诊断,但对于血清总前列腺特异性抗原(total prostate-specific antigen 2, tPSA)处于4～10 ng/mL诊断灰区的前列腺癌,其诊断意义有限。为提高了前列腺癌患者PSA处于灰区时的诊断率,减少不必要的有创穿刺操作,本文就前列腺特异性抗原同源异构体2(prostate-specific antigen isoform 2, p2PSA)及前列腺健康指数(prostate health index, PHI)对于早期有临床意义前列腺癌(clinically significant prostate cancer, csPCa)诊断的临床应用价值,与前列腺癌的侵袭性,以及对于前列腺癌患者的疾病进展、预后、复发等方面都具有潜在的作用进行论述。近些年,多参数磁共振成像(mpMRI)也成为了预测csPCa的常用工具,使用MRI和PHI相互补充可用于检测早期有临床意义的前列腺癌。

Objective: To analyze the effects of Helicobacter pylori on blood pressure, carotid atherosclerosis, long non-coding RNA (LncRNA) MALAT1, nitric oxide (NO), corticosterone and new cardiovascular and cerebrovascular diseases. Methods: The patients with both hypertension and Helicobacter pylori infection were divided into experimental group and control group. 296 cases each.The control group was treated with antihypertensive and lipid-lowering treatment, and the experimental group was treated with anti-Helicobacter pylori drugs on this basis. Then the changes of blood pressure, blood lipid level, carotid intima thickness and heart rate were compared between the two groups. After 2 years of follow-up, the proportion of new cardiocerebral artery occlusion was observed. Thirty-two 6-week-old male rats were divided into spontaneously hypertensive rats (SHR) group A and group B, Wistar-Kyoto (WKY) rats group C and group D, with 8 rats in each group. Rats in group A and group C were given Helicobacter pylori gavage, and rats in group B and group D were given 0.9% NaCl gavage. After 14 weeks, blood and gastric tissue were taken for detection. Results: (1) Helicobacter pylori affected blood pressure, so that the blood pressure of SHR and WKY rats were significantly increased; the degree of hypertension in SHR was more obvious (P＜0.05). (2)The increase of Helicobacter pylori value affected the levels of LncRNA MALAT1, NO and corticosterone (P＜0.05). (3) The proportion of new heart and cerebral artery insufficiency or even occlusion after the decrease of Helicobacter pylori value was less than that of the control group (P＜0.05). Conclusion: (1)The content of Helicobacter pylori is positively correlated with the increase of blood pressure, LncRNA MALAT1 and corticosterone, and negatively correlated with NO. (2) After the treatment of Helicobacter pylori, it can reduce the degree of blood pressure level and the proportion of coronary cerebral artery occlusion.

Objective: To identify the related genes in patients with childhood asthma (CA) based on bioinformatics and immunoassay, and to explore its potential value in the diagnosis of CA. Methods: The GSE27011 data set was downloaded from the Gene Expression Omnibus (GEO) database, and the differentially expressed genes were screened by RStudio. The immune differences between the disease group and the normal group were analyzed respectively. The PPI network was drawn and the Hub genes were screened. The Hub genes were used to select the genes most related to immunity, and the prediction model was constructed by using the screened genes to make a nomogram. Finally, the independent GSE18965 data set was selected for verification. Results: A total of 37 differentially expressed genes were obtained. The difference analysis of immune cells showed that the levels of natural killer cells and plasma cell-like dendritic cells in CA were higher, and the difference of immune function showed that the cytotoxic activity of CA and the level of promoting inflammatory immune function were higher. Ten Hub genes were selected through the PPI network, and then five genes most related to immunity were screened, namely KLRF1, KLRD1, PRF1, IL2RB, and NCAM1. Using five genes to construct models and verify, it was found that KLRF1, KLRD1, PRF1 genes and CA had high predictive diagnostic value. Conclusion: The identified KLRF1, KLRD1 and PRF1 genes may be biomarkers for the diagnosis of CA, and natural killer cells may be a potential therapeutic target for CA.