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  • CHEN Xinnan, LI Zengyan
    Journal of Baotou Medical College. 2023, 39(9): 91-96. https://doi.org/10.16833/j.cnki.jbmc.2023.09.018
    Wnt蛋白家族属于一类具有高度保守半胱氨酸残基的分泌性脂质修饰糖蛋白,其通路是一组高度保守的信号转导通路,能够介导多种生理和病理过程,如参与调控细胞增殖、分化、凋亡、免疫与应激、炎症和纤维化。目前认为Wnt信号通路包括三个分支:经典Wnt信号通路,即Wnt/β-Catenin信号通路;Wnt/PCP通路;Wnt/Ca2+通路。Wnt/β-catenin信号途径在心、肝、肺、肾等器官纤维化等病理生理过程中均发挥了重要作用。越来越多的证据表明,Wnt/β-catenin信号通路在糖尿病肾病(diabetic nephropathy, DN)的进展中意义重大。本文就Wnt/β-catenin在系膜细胞凋亡、上皮间充质转变(epithelial mesenchymal transition, EMT)、DN条件下足细胞功能障碍、小管EMT和肾纤维化中的作用进行综述。此外,还介绍了这一过程所涉及的分子机制。这一信息为Wnt/β-catenin保护肾脏损伤和延缓DN进展提供了一个新的分子靶点。
  • WU Xiaorong, ZHU Shilong, GONG Shiji, YANG Zhanjun, JIA Jianxin
    Journal of Baotou Medical College. 2023, 39(11): 1-5. https://doi.org/10.16833/j.cnki.jbmc.2023.11.001
    Objective: To clarify whether general cistanosides (GCs) ameliorates cognitive dysfunction in rapidly aging mice (SAMP8) by mediating specific androgen receptor (AR) in regulation of expression of synaptic protein. Methods: A total of 76 7-month-old male SAMP8 mice were randomly divided into the model group, GCs group, GCs + flutamide (F) group, GCs + fulvestrant (ICI) group, F group, and ICI group. The learning and memory functions of mice in each group were tested with Morris water maze. Western blotting and RT-PCR were used to test the expression level of synaptophysin (SYN), postsynaptic density-95 (PSD-95) and the brain-derived neurotrophic factor (BDNF) of mice in each group. Results: GCs significantly improved the learning and cognitive function of SAMP8 mice. Comparing with the GCs group, the learning and memory function of mice in the GCs+F group and GCs+ICI group was significantly lowered. Western blotting and RT-PCR results showed that the expression level of BDNF, SYN, and PSD-95 were significantly increased at the protein level and gene level in the GCs group compared with the Model group (P<0.05). SYN expression level was increased in the GCs+F and GCs+ICI group, and expression levels of BDNF and PSD-95 were significantly decreased in the GCs+F and GCs+ICI group compared with the GCs group (P<0.05). Conclusion: GCs may specifically mediate AR to enhance synaptic plasticity in SAMP8 mice, which in turn improve learning and cognitive function.
  • OU Haiqiang, LI Hui, LU Xiaoshan
    Journal of Baotou Medical College. 2023, 39(11): 47-50. https://doi.org/10.16833/j.cnki.jbmc.2023.11.009
    Objective: To compare the short-term pain response and long-term clinical efficacy of iRoot SP and AH-PLUS as apical sealers. Methods: A total of 96 single-rooted permanent teeth of 90 patients with chronic periapical periodontitis from August 2017 to December 2018 were randomly selected as the study objects. After routine preparation of root canal, they were divided into the iRoot SP group and AH-PLUS group based on different apical sealers they used. The pain response of the two groups 1 week after surgery was compared, and clinical efficacy 6 months after surgery of the two groups were compared. Results: The pain degree in the AH-PLUS group was slightly higher than that in iRoot SP group 1 week after surgery, and the difference was statistically significant (P<0.05). The accurate filling rate in the iRoot SP group was higher than that in the AH-PLUS group (P<0.05). Six months after root canal filling, there was a statistically significant difference between the two groups in the treatment effect (P<0.05). The success rate of the iRoot SP group was higher than that of the AH-PLUS group (P<0.05). Conclusion: iRoot SP has less pain stimulation to periapical tissues 1 week after surgery, better filling effect and long-term clinical efficacy than the AH-PLUS, it could be widely used in clinical practice.
  • LIN Yufeng, ZHAO Rui, CHEN Xianghui, ZHU Wei, LI Xiaofeng, XIE Yabin, XIE Wei, BADE Rengui, JIANG Shuyuan, LIU Xiaolei, SHAO Guo, BAI Haihua, JIA Xiaoe
    Journal of Baotou Medical College. 2023, 39(8): 1-5. https://doi.org/10.16833/j.cnki.jbmc.2023.08.001
    Objective: To observe the regenerative effect of Mongolian medicine Eerdun Wurile (EW) on zebrafish heart injury and its possible mechanism, and provide experimental basis for further research on Mongolian medicine Eerdun Wurile. Methods: The animal model of ventricular injury was established using the transgenic line Tg (vmhc: mCherry) treated with metronidazole (MTZ). The zebrafish embryos at 3 dpf(days post fertilization) were randomly divided into the control group (DMSO group,n=30), the experimental myocardial ablation group (MTZ group, n=30) and the Eerdun Wurile treatment group (MTZ+EW, n=30) after ventricular injury. Three groups were treated with 25 ug/ml Eerdun Wurile, a Mongolian medicine, after MTZ treatment for 24 hours. Three groups were tested for cell proliferation at 72 hpt. Cell proliferation were observed with confocal microscope and quantitatively analyzed. At the same time, NOS, iNOS, TNF-α, IL-1β and IL-6 were analyzed with real time qPCR. Results: After the treatment with Mongolian medicine Erden-Uhelle, the proportion of regeneration and repair after myocardial injury was significantly increased in the treatment group. And the proliferation of regenerated myocardial cells was enhanced in Eerdun Wurile treated group. The expression of inflammatory factors was significantly lower than that of the control group. Conclusion: Mongolian medicine Eerdun Wurile can promote the repair and regeneration of zebrafish embryonic cardiac muscle cells, promote the proliferation of cardiac muscle cells, and inhibit the inflammatory reaction after zebrafish embryonic ventricular injury.
  • ZHENG Yuanhai, LIN Yuanfeng, FANG Xiaoli, CAI Yirong, WEN Xiaofang
    Journal of Baotou Medical College. 2023, 39(8): 59-62. https://doi.org/10.16833/j.cnki.jbmc.2023.08.012
    Objective: To investigate the diagnostic value of peripheral blood neutrophil CD64 index (nCD64IND), monocyte CD14 index (mCD14IND) and interleukin 6 (IL-6) levels in adult pulmonary infection. Methods: A total of 63 adult patients with pulmonary infection hospitalized from December 2020 to February 2021 were selected as the study group and 47 healthy adults as the control group. The levels of nCD64IND, mCD14IND and IL-6 in peripheral blood were determined by flow cytometry. Pearson's method was used to analyze the correlation between the three indices and the common indicators of pulmonary infection, such as CRP and PCT. The ROC curve was drawn to evaluate the sensitivity and specificity of the three indices in the diagnosis of pulmonary infection. Results: The levels of nCD64IND, mCD14IND and IL-6 in peripheral blood of adult patients with pulmonary infection were significantly higher than those of healthy control group (all P<0.05). Correlation analysis showed that the levels of nCD64IND and IL-6 in peripheral blood of patients were positively correlated with CRP, mCD14IND was negatively correlated with CRP, and the three indices were not significantly correlated with PCT. ROC curve analysis showed that the sensitivity of nCD64IND, mCD14IND and IL-6 levels in the diagnosis of lung infection was 81 %, 58.7 % and 84.1 %, respectively, and the specificity was 89.4 %, 78.7 % and 89.4 %, respectively. Conclusion: The determination of nCD64IND, mCD14IND and IL-6 in peripheral blood has certain diagnostic value for pulmonary infection.
  • LI Tingyu, LUO Sumei, SHI Hui
    Journal of Baotou Medical College. 2023, 39(10): 91-96. https://doi.org/10.16833/j.cnki.jbmc.2023.10.018
    Rheumatoid arthritis is a chronic systemic autoimmune disease. The inflammatory response caused by the abnormal activation of the immune system will lead to the formation of pannus in the synovium of the joints, and the gradual destruction of bone and cartilage. The clinical manifestations are pain and swelling of multiple joints, or even deformity, affect the quality of life of patients, and eventually lead to loss of joint function. The main reason for its onset and recurrence is that the immune system produces immune cells, cytokines and antibodies against self-antigens, among which the NLRP3 inflammasome plays a key role in the inflammatory response. As a binding protein of NLRP3, NEK7 kinase acts downstream of K+ efflux and regulates the oligomerization and activation of NLRP3 inflammasomes. However, there have been no report on the involvement of NEK7 in the inflammatory activities of rheumatoid arthritis through the activation of NLRP3 inflammasomes. This article reviews the research progress of NLRP3 inflammasome activation and NEK7 kinase regulating the process of NLRP3 inflammasome activation, in order to provide new insights for the understanding of NLRP3 inflammasome activation by NEK7 on inflammatory activity in rheumatoid arthritis and future research related to targeted therapy.
  • LI Xiaofeng, LIN Yufeng, ZHANG Yabei, CHEN Xianghui, ZHAO Rui, ZHU Wei, XIE Yabin, XIE Wei, BADE Rengui, JIANG Shuyuan, LIU Xiaolei, SHAO Guo, BAI Haihua, JIA Xiaoe, YANG Zhifu
    Journal of Baotou Medical College. 2024, 40(1): 1-7. https://doi.org/10.16833/j.cnki.jbmc.2024.01.001
    Objective: To explore the main active components and drug targets of Mongolian medicine Erdun-Wurile in the treatment of Parkinson’s disease and its potential action mechanism based on the network pharmacology, and study the binding ability of drugs and target proteins with molecular docking. Methods: The active components and corresponding targets of Eerdun-Wurile were mined based on the traditional Chinese medicine systems pharmacology database and analysis platform, TCMCSP). The mined data were screened under the term of pharmacokinetic parameters [oral bioavailability (OB)≥30%, drug-likeness property (DL)≥0.18]. GeneCards, OMIM and TTD databases of human gene-disease related databases were used to screen out the possible targets in active components of Eerdun-Wurile in the treatment of Parkinson’s disease. The Venn diagram was drawn by Cytoscape 3.9.1, and PPI network diagram was drawn with STRING database. The Bioinformatics and Metascape database were used to search the potential active ingredients of Erden-Wurile and potential targets for the treatment of Parkinson’s disease, and GO function analysis and KEGG enrichment analysis was performed on screened targets with molecular docking of small drug molecules and target proteins. Results: 118 kinds of active ingredients with good DL and oral absorption were screened from Eerdun-Wurile medicine, and 207 targets were predicted to treat Parkinson’s disease. GO function analysis and KEGG enrichment analysis showed that the mechanism of Mongolian medicine Eerdun-Wurile in treating Parkinson’s disease mainly involved with biological processes such as positive regulation of RNA polymerase II promoter transcription, positive regulation of DNA template transcription, positive regulation of gene expression. Pathways involved were AKT1, TP53, TNF, VEGFA, CASP3, etc. Conclusion: The effect of Mongolian medicine Eerdun-Wurile in treating Parkinson’s disease is the action result of multi-components, multi-targets and multi-channels, which provides theoretical basis for further research on treating Parkinson’s disease with Eerdun-Wurile.
  • ZHU Hao, FENG Ke, WANG Yani, XU Tengyue, YANG Guang, YANG Chenhui, ZHANG Shuting, HAN Wenyan
    Journal of Baotou Medical College. 2023, 39(10): 69-75. https://doi.org/10.16833/j.cnki.jbmc.2023.10.014
    Objective: To investigate the current situation of depression, anxiety and stress of students in a university, and to provide a basis for the school to understand the psychological status of students. Methods: From November 1 to November 30,2021, a stratified cluster random sampling method was used to investigate the depression, anxiety and stress status of undergraduate, junior college and graduate students in Jinshan Campus of Inner Mongolia Medical University. The survey tool consists of two parts: a self-made general situation questionnaire and a Self-rating Depression-anxiety-stress Scale (DASS-21). The general situation questionnaire includes demographic characteristics, economic status, self-rated health status, stress status, interpersonal relationship, exercise status, mobile phone / network use status, etc. Results: Of the 1569 participants, 21.73 % were found to be depressed, 29.32 % were anxious, and 11.03 % were stressed. The results of stratified analysis and multivariate analysis showed that the protective factors of depression were : female (OR=0.68, P<0.01), physical exercise (OR=0.81, P<0.01), good physical condition (OR=0.53, P<0.01), good learning status (OR=0.90, P<0.05), good interpersonal relationship (OR=0.75, P<0.01), and the higher the grade, the higher the depression rate of the students. The protective factors of anxiety were good physical condition (OR=0.54, P<0.01) and good interpersonal relationship (OR=0.77, P<0.05). The protective factors of stress were female (OR=0.71, P<0.05), good physical condition (OR=0.60, P<0.01), more physical exercise (OR=0.70, P<0.01) and good interpersonal relationship (OR=0.81, P<0.01). Conclusion: The incidence of anxiety among college students is relatively high. Schools should take the initiative to regularly monitor the psychological status of students. Setting up interpersonal relationship counseling courses and carrying out indoor physical exercise in winter may help to slow down the occurrence of students ' psychological problems.
  • WANG Yanfang, SONG Kuan, SONG Zhaohe, ZHANG Hongli, MA Yong, WEI Simeng
    Journal of Baotou Medical College. 2023, 39(11): 92-96. https://doi.org/10.16833/j.cnki.jbmc.2023.11.018
    Objective: To investigate the antidepressive effect of Qingxin Chenxiang Bawei Powder and its effect on monoamine neurotransmitters.Methods: Kunming male mice were randomly divided into the blank control group (distilled water), positive-drug group (fluoxetine hydrochloride 0.02 g/kg) and Mongolian medicine Qingxin Chenxiang Bawei Powder groups [the high-dose (27 g/kg) group, medium-dose (9 g/kg)group and low-dose (3 g/kg) group].Intragastric administration were performed on all experimental mice.Mice tail suspension test (TST) and forced swimming test (FST) were used to evaluate the antidepressive effect of the drug 7 days after intragastric administration.After FST behavioral experiment, the contents of 5-HT, DA and NE in plasma and hippocampus were determined by enzyme-linked immunosorbent assay (ELISA).Results: Compared with the blank control group, medium-dose and low-dose groups of Mongolian medicine could significantly shorten the immobility time in mice FST, and the difference was statistically significant (P<0.05).The immobility time in mice TST was significantly shortened in the low-dose group, and the difference was statistically significant(P<0.05).The plasma DA level was significantly increased in the low-dose group, and the difference was statistically significant (P<0.05).The levels of 5-HT, DA and NE in the hippocampus of mice in the low-dose, medium-dose and high-dose groups were significantly increased, and the difference was statistically significant (P<0.05).Conclusion: Qingxin Chenxiang Bawei Powder have certain antidepressive effect on mice, which may be related to its regulation effect on monoamine neurotransmitter content.
  • ZHONG Jieyi
    Journal of Baotou Medical College. 2023, 39(11): 55-58. https://doi.org/10.16833/j.cnki.jbmc.2023.11.011
    Objective: To explore the prognostic significance of D-dimer, routine coagulation tests and platelet parameters in pregnant women. Methods: A total of 454 cases of pregnant women who came to Guangdong Tongjiang Hospital for examination and delivery from January 2020 to June 2021 were selected as the normal pregnancy group (96 cases in the first trimester, 138 cases in the second trimester, 114 cases in the third trimester, and 106 cases in the parturient period), another 60 cases of pregnancy with other diseases such as gestation combining with diabetes or hypertension were selected as the other-disease-combining group, and 45 cases of obstetric accidents were collected as the obstetric accident group. In addition, 167 cases of non-pregnant women in childbearing age who came to our hospital for health examination were selected as the healthy control group. The D-D level and coagulation tests including activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FIB), and thrombin time (TT) were compared among the four groups. The platelet parameters including platelet count (PLT), mean platelet volume (MPV), and platelet volume distribution width (PDW) were also compared among the four groups. Results: The levels of D-D and FIB in the normal pregnancy group were significantly lower than those in the other-disease-combining group and the obstetric accident group (P<0.05). PT level in the normal pregnancy group was significantly higher than that in the other-disease-combining group and the obstetric accident group (P<0.05). The PLT, MPV and PDW levels in the normal pregnancy group were significantly higher than those in the other-disease-combining group and the obstetric accident group (P<0.05). Conclusion: Four indexes of blood coagulation, D-D and platelet parameters can effectively monitor the state of blood coagulation of pregnant women before delivery, which is of great significance in mastering the coagulation condition, improving the pregnancy outcome and preventing maternal thrombosis or postpartum hemorrhage, it is worthy of popularization and clinical promotion.
  • ZHANG Wei, WANG Yin, WEN Zhen ping
    Journal of Baotou Medical College. 2023, 39(11): 14-22. https://doi.org/10.16833/j.cnki.jbmc.2023.11.003
    Objective: To screen piRNA pathway related genes associated with diagnosis and prognosis of colorectal cancer (CRC). Methods: The expression of 39 piRNA pathpathy-related genes at the tissue level was analyzed based on 647 colorectal tumor samples and 51 non-tumor samples from the Cancer Genome Atlas (TCGA) database, and the risk prediction model was constructed. KM and ROC were used to evaluate the prediction performance and effect of the measurement model. Finally, candidate molecules were selected based on clinicopathological features and validated at the cellular level. Results: Among 37 piRNA pathway related genes with significant differences, 8 genes co-constructed the risk prediction model. KM analysis showed that OS decreased significantly in high-risk group. The ROC curve showed that the area under the curve of the model was 0.624, 0.669, and 0.670 in 1, 3, and 5 years respectively. The expression levels of POLR2F and PAICS were overexpressed in colorectal cancer cells and CTPAC Database data, and correlated with clinicopathological features. In particular, the expression level of PAICS was positively correlated with tumor immune infiltrating cells in CRC, and significantly lower in cancer-associated fibroblasts. Conclusion: piRNA pathway related genes, especially POLR2F and PAICS, may be the potential biomarkers for cancer diagnosis and prognosis in CRC.
  • CHEN Lijuan, YUAN Xia, LIU Qingqing
    Journal of Baotou Medical College. 2023, 39(9): 70-73. https://doi.org/10.16833/j.cnki.jbmc.2023.09.014
    Objective: To analyze the effect of the adjuvant therapy with bronchoalveolar lavage, pulmonary surfactant protein,lactate dehydrogenase (LDH), penetrating hormone 3 (PTX3) level, and arterial blood gas indexes in children with refractory mycoplasma pneumonia (RMPP). Methods: Clinical data of 97 children with RMPP in our hospital from October 2016 to June 2022 were collected retrospectively, and divided into the drug treatment group (48 cases) and bronchoalveolar lavage group (49 cases) according to the different treatment methods. The children in the drug treatment group were treated with macrolide for anti-infection and symptomatic treatment. The children in the bronchoalveolar lavage group were treated with bronchoscopic bronchoalveolar lavage on the basis of the drug treatment group. The curative effect, the levels of pulmonary surfactant protein A (SP-A), pulmonary surfactant protein D (SP-D), LDH and PTX3 and arterial blood gas indexes before and after treatment of the two groups were compared. Results: The total effective rate in the bronchoalveolar lavage group was 89.80 %, which was obviously higher than that in the drug treatment group (70.83 %) (P<0.05). Before treatment, the levels of SP-A, SP-D, LDH, PTX3 and arterial blood gas indexes had no significant difference between the two groups (P>0.05). After treatment, the levels of SP-A, SP-D, LDH and PTX3 in the bronchoalveolar lavage group were obviously lower than those in the drug treatment group, and the arterial blood gas indexes were obviously better than those in the drug treatment group (P<0.05). The incidence of adverse reactions in the bronchoalveolar lavage group (10.00 %) was obviously lower than the drug treatment group (26.00 %) (P<0.05). Conclusion: The adjuvant therapy with bronchoalveolar lavage in the treatment children with RMPP has a good treatment effect, which could significantly improve the arterial blood gas indexes and lower the levels of pulmonary surfactant protein (SP-A, SP-D), LDH and PTX3 with high safety.
  • HU Wenhui, JU Hongge, LI Feng, ZHANG Wei, MA Junbing, CHEN Peng, LI Yuanyuan, SUN Yang
    Journal of Baotou Medical College. 2023, 39(9): 1-7. https://doi.org/10.16833/j.cnki.jbmc.2023.09.001
    Objective: To observe the expression of oncogene c-Fos, cyclin D1 and cyclin dependent kinase 4 (CDK4) in breast cancer and para-cancerous tissues and their correlation with clinicopathological features, and to analyze the survival of breast cancer. Methods: Western blot (WB) and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) were used to detect the expression level of c-Fos protein and mRNA content of c-Fos, Cyclin-D1 and CDK4 in 20 cases of breast cancer and para-cancerous tissues. Breast tumor-on-a-chip was purchased to do immunohistochemical (IHC) experiment to analyze the relationship between expression levels of c-Fos, Cyclin-D1 and CDK4 and the clinicopathological features and prognosis of breast cancer. Results: WB test showed that the expression level of c-Fos protein in breast cancer was significantly higher than that in para-cancerous tissues, and the difference was statistically significant(P<0.05). RT-qPCR method showed that the mRNA content of c-Fos in breast cancer was higher than that in para-cancerous tissues, however, the difference was not statistically significant(P>0.05). The mRNA contents of Cyclin-D1 and CDK4 in breast cancer were significantly higher than those in para-cancerous tissues(P<0.05). IHC experiment showed that the expression levels of c-Fos, Cyclin-D1 and CDK4 in breast cancer tissues were significantly higher than those in para-cancerous tissues, and the differences were statistically significant (P<0.05). The expression levels of the three proteins in cancer tissues were all positively correlated(r=0.320,r=0.486,r=0.514,P<0.05). In breast cancer, the expression level of c-Fos was correlated to clinical stage(P<0.05), the expression level of Cyclin-D1 was correlated to vascular invasion(P<0.05), the expression level of CDK4 was related to lymph node metastasis(P<0.05), and the high expression of the three proteins was the risk factor to the over survival of breast cancer patients. Conclusion: c-Fos, Cyclin-D1 and CDK4 are highly expressed in breast cancer, and there is a positive correlation among them, which indicates that they involved in the occurrence and development of breast cancer. Highly expressed c-Fos, Cyclin-D1 and CDK4 is positively correlated with clinicopathological features of breast cancer, which is a risk factor to the prognosis of patients with breast cancer.
  • GAO Han, JIN Guoxi, WANG Huimin, PEI Xiaoyan
    Journal of Baotou Medical College. 2023, 39(11): 32-36. https://doi.org/10.16833/j.cnki.jbmc.2023.11.006
    Objective: To investigate the relationship between serum fibroblasts growth factor (FGF) 19 level and the development of atherosclerosis (AS) in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 78 T2DM patients were selected and divided into two groups based on carotid intima-media thickness (CIMT). Among them, 40 T2DM patients were control group and 38 T2DM patients with AS were experimental group. Record the blood pressure and body mass index (BMI) of all inclusive patients. Detection of related biochemical indicators such as blood sugar, blood lipids. Serum FGF19, heme oxygenase-1 (HO-1),malondialdehyde (MDA), glutathione peroxidase (GSH-Px) levels were measured by enzyme-linked adsorption immunosorbent assay (ELISA), and superoxide peroxide dismutase (SOD) level was determined by biochemical method. The differences in outcome measures between the two sets were compared. The correlations between FGF19 level and HO-1, SOD, MDA, GSH-Px, CIMT and brachial ankle pulse wave velocity (baPWV) were analyzed by Pearson correlation analysis. Multiple linear regression analysis was performed to analyze the influencing factors of FGF19. Results: Compared with the control group, CIMT, baPWV, HO-1, MDA increased in the experimental group (P<0.01), FGF19, SOD and GSH-Px decreased significantly compared with the control group (P<0.01). Correlation analysis showed that the serum FGF19 level of the experimental group was negatively correlated with HO-1, MDA, CIM, baPWV(r=-0.668、-0.657、-0.901、-0.734,P<0.01) were positively correlated with SOD and GSH-px (r=0.345、0.760,P<0.01).Multiple linear regression analysis showed that FGF19, HO-1, SOD, MDA, GSH-PX were all the possible influencing factors of CIMT (P<0.05). Conclusion: Serum FGF19 level in T2DM patients has a certain relationship with oxidative stress, and has important clinical significance in predicting the occurrence and progression of AS.
  • TANG Na, ZHANG Wenlan, BAI Li, PANG Chunyan, WANG Yongfu, ZHANG Wei, HU Tongping
    Journal of Baotou Medical College. 2023, 39(10): 18-22. https://doi.org/10.16833/j.cnki.jbmc.2023.10.004
    Objective: To investigate the value of circulating cell-free DNA (cfDNA) in the early diagnosis and disease activity of patients with rheumatoid arthritis (RA). To explore the relationship between cfDNA content and immunological diagnostic indicators of RA and related indicators of disease activity evaluation (rheumatoid factor (RF), Anti-cyclic citrullinated peptide antibodies (anti-CCP antibodies), C-reactive protein (CRP), Erythrocyte sedimentation rate (ESR)) and Disease Activity Score of 28 joints (DAS28-ESR). Methods: A total of 80 newly diagnosed RA patients who did not receive anti-rheumatic drugs were selected as the observation group (RA group), and 40 healthy people were selected as the control group (HC group). The serum cfDNA content of two groups was measured and the integrity was calculated. The correlation between cfDNA content and clinical indicators of RA patients was analyzed. Results: The RA group had a significantly higher level of cfDNA than the HC group (P<0.05), but there was no correlation between and diagnostic serological markers (P>0.05). According to the disease activity index of 28 joints (DAS28-ESR score), RA patients were stratified by disease activity, and it was found that the cfDNA content in the high DAS28-ESR score group was higher than that in the low DAS28-ESR score group (P<0.05). cfDNA was correlated with DAS28-ESR, CRP and ESR (P<0.05). Conclusion: The content of serum cfDNA in RA patients is increased, which is correlated with inflammatory response. It has diagnostic value for RA and may be an independent risk factor for the pathogenesis of RA.
  • SUO Yaowen, MO Chaoran, YANG Zhanjun
    Journal of Baotou Medical College. 2023, 39(12): 1-7. https://doi.org/10.16833/j.cnki.jbmc.2023.12.001
    Objective: To study the mechanism of total flavonoids of Dracocephalummoldavica L. (TFD) on improving nerve injury in Parkinson's disease model mice. Methods: A total of 120 mice were randomly divided into normal control group (n=20) and observation group (n=100). The mice in the observation group were intraperitoneally injected with 30 mg/(kg·d) PTP to prepare PD mouse model, and the mice in the control group were intraperitoneally injected with the same volume of normal saline. After 7 days, the mice in the observation group were randomly divided into 5 groups : model group and TFD treatment group [25, 50, 75, 100 mg/(kg·d )]. The mice in the treatment group were given intragastric administration according to the body weight of the mice, and the mice in the normal control group and the model group were given an equal volume of normal saline for 1 month. The motor function of mice was evaluated by behavioral experiments. The number of positive staining cells of tyrosine hydroxylase (TH) and selective autophagy adaptor protein (P62) in the substantia nigra of mice was detected by immunofluorescence histochemistry. The expression levels of microtubule-associated protein 1 light chain 3β (LC3B), P62, autophagy effector protein (Beclin1) and TH mRNA and protein in the substantia nigra of mice were detected by fluorescence quantitative PCR and Western-blot. Results: TFD treatment significantly increased the suspension motion score, movement distance and fall latency of PD model mice (P<0.05). TFD treatment increased the number of positive staining cells expressing P62 and TH in the substantia nigra of PD model mice, and TFD treatment increased the number of positive staining cells expressing PINK1, Parkin, P62 and TH proteins in the substantia nigra of PD model mice (P<0.05). Conclusion: TFD may improve autophagy by up-regulating LC3B, P62 and Beclin1, and promote the clearance of damaged mitochondria and other organelle of dopaminergic neurons, thus producing neuroprotective effects on MPTP-induced PD model mice.
  • ZHANG Yunqiu, ZHANG Xiangeng, CAO Bing, WANG Hongyan, LIU Yunqi, ZHOU Fan, WANG Yuting
    Journal of Baotou Medical College. 2023, 39(11): 64-69. https://doi.org/10.16833/j.cnki.jbmc.2023.11.013
    Objective: To understand the willingness to participate elderly care of elderly people who live alone and explore its influencing factors, with the purpose of providing basis for the establishment of a robust social system of elderly care service.Methods: The data of 1 208 elderly people who live alone from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) were collected, using Andersen's model as a framework and 2 test to analyze the difference in the choice of elderly care mode among the elderly with different characteristics. Propensity, enabling resources, and needs were analyzed with binary logistic regression analysis to determine the influencing factors of willingness to participate elderly care of elderly people who live alone. Results: Among the 1 208 elderly living alone, the 92.0 % of elderly people preferred community service for home care, and only 8.0 % of elderly people willing to be admitted to institutional care for elderly. Logistic regression analysis showed that older people with an education level of elementary school, general income level, or having more than one child who live alone had lower willingness to institutional care for elderly (OR<1,P<0.05). The elderly living alone who were routinely cared for by related institutions, with more loneliness and poor health status, and very restricted in their ability to perform daily living, were more likely to choose an institutional care for elderly value (OR>1, P<0.05). The determination coefficients R2 of models Ⅰ to Ⅲ increased stepwise, among which the enabling factors contributed to the most to the model. Conclusion: The willingness to participate elderly care of elderly people who live alone is mainly preferred to community service for home care. The willingness to participate elderly care of elderly people who live alone is affected by several factors, of which the enabling factors have the greatest influence. In addition to enhance community service for home care, a diverse and precise model of elderly care should be explored to meet the needs of elderly people who live alone.
  • WANG Jun, ZHANG Ruimin, WU Xiangming, MIAO Wei, ZHAO Yongxiang
    Journal of Baotou Medical College. 2023, 39(11): 37-41. https://doi.org/10.16833/j.cnki.jbmc.2023.11.007
    Objective: To investigate the correlation between primary intussusception and food allergen-specific IgE, food-specific IgG and vasoactive intestinal peptide. Methods: A total of 35 children with intussusception admitted to the Fourth Hospital of Baotou from October 2021 to May 2022 were selected into the experimental group, and 35 children with no intestinal diseases at the same period were selected into the control group. The levels of 7 food allergen-specific IgE antibodies, food-specific IgG antibodies and serum vasoactive intestinal peptide of children between the two groups were compared. Results: The total positive rate of food allergen-specific IgE in children of the experimental group was 40.00 % and 28.57 % in the control group, and the differences in positive rate and total positive rate of food allergen-specific IgE of the two groups were not statistically significant (P>0.05). The total positive rate of food-specific IgG was 88.57 % in the children of the experimental group and 62.86 % in the control group, and the difference was statistically significant (P<0.05), but the difference in the positive rate of various types of food-specific IgG in the two groups was not statistically significant (P>0.05). The level of vasoactive intestinal set was lower in the experimental group than that in the control group, and the difference was statistically significant (P<0.05). The difference in serum vasoactive intestinal peptide levels between food-specific IgG-positive and negative children was statistically significant (P<0.05), and there was a negative correlation between food-specific IgG and vasoactive intestinal peptide (P<0.05). Conclusions: No significant correlation was seen between food allergen-specific IgE and the occurrence of primary intussusception, while food-specific IgG was correlated with the occurrence of primary intussusception. The changed levels of vasoactive intestinal peptides in the serum of children with intussusception suggest that the level of vasoactive intestinal peptide is related to the occurrence of primary intussusception, and that food-specific IgG may affect intestinal function by regulating the levels of vasoactive intestinal peptides, which ultimately lead to the occurrence of primary intussusception.
  • NIU Lu, MENG Xianmei, SHAO Guo
    Journal of Baotou Medical College. 2023, 39(9): 34-39. https://doi.org/10.16833/j.cnki.jbmc.2023.09.007
    Objective: To elucidate the differentially expressed genes in reflux esophagitis and explore the possible mechanism of mucosal damage in reflux esophagitis, and preliminarily screen and identify the expression changes and effects of long-chain non-coding RNA HOTAIR in reflux esophagitis. Methods: Transcriptome sequencing and bioinformatics analysis were performed on tissue specimens at mucosal erosion site of reflux esophagitis and mucosa smooth esophageal tissue under para-erosion endoscopy. The expression changes of long-chain non-coding RNA HOTAIR in the two types of tissues were determined by real-time fluorescent quantitative PCR, and its relationship with the age of patients with reflux esophagitis was analyzed. Results: The transcriptome sequencing results showed that there were a variety of genes differentially expressed between the erosive group and the para-erosive group of reflux esophagitis, of which 7661 genes were down-regulated. Bioinformatics analysis showed that differentially expressed genes were enriched in immune responses. For the screened long-chain non-coding RNA HOTAIR, real-time fluorescent quantitative PCR showed that its relative expression was correlated with the age of patients with reflux esophagitis (P<0.05). Compared with the para-erosion group, the expression of HOTAIR was up-regulated in the erosion group of reflux esophagitis in the older age group (P<0.05), and down-regulated in the erosion group of reflux esophagitis in the younger age group (P<0.05). Conclusion: The differentially expressed genes may be involved in the pathogenesis of reflux esophagitis through immune response. The screened long-chain non-coding RNA HOTAIR expression showed opposite trends in patients of different ages, suggesting that HOTAIR may play an important role in reflux esophagitis and can be used as an important auxiliary indicator for diagnosis.
  • LI Puchao, LIU Quanli
    Journal of Baotou Medical College. 2023, 39(10): 79-83. https://doi.org/10.16833/j.cnki.jbmc.2023.10.016
    Objective: The formulation process of torasemide injection was optimized. Methods: The formulation and preparation technology of torasemide injection were determined by screening the types of excipients, the range of pH value, the proportion of activated carbon dosage and sterilization conditions. Results: The best prescription is torasemide 5 g, polyethylene glycol 400 100 g, tromethamine 2 g, sodium hydroxide 0.5 g, water for injection 1 000 mL. The range of pH value was determined to be 9.00±0.20; the dosage of activated carbon was 0.10 %; the sterilization conditions were 121 ℃ for 15 min. The results of process verification and stability test show that the prescribed process is feasible. Conclusion: The torasemide injection product determined by this prescription and technology has stable quality and good safety.