Objective: To study and analyze the changes of intestinal flora in 1-5 years after cholecystectomy. Methods: A total of 15 fecal samples of healthy people and 16 fecal samples of people 1 to 5 years after cholecystectomy were collected from Baotou Central Hospital. The collected samples were sequenced to analyze the composition, richness and diversity of intestinal microbial flora in the two groups, and to compare the differences of intestinal microbial flora between the two groups. Results: (1) At the phylum level, the relative abundance of Bacteroidetes, Proteobacteria, Verrucomicrobia and Fusobacteria increased, while the relative abundance of Actinobacteria decreased in the population 1-5 years after cholecystectomy. (2) At the genus level, the relative abundance of Escherichia, Ruminococcus, Blautia, and Gemmatiaceae increased, while the relative abundance of Faecalibacterium, Bifidobacterium, Collins, and Agathobacter decreased in the population 1 to 5 years after cholecystectomy. The Chao1 index and Observed _ species index of the healthy population group were higher than those of the population group 1-5 years after cholecystectomy, and the differences were statistically significant (P＜0.05). The Pielou evenness of the healthy population group was slightly lower than that of the population group 1-5 years after cholecystectomy, and the difference was statistically significant (P＜0.05). Lachnospiraceae, Ruminococcus, Faecalimonas and Burkholderia were the intestinal flora with significant differences between the groups in the population group from 1 to 5 years after cholecystectomy. Conclusion: (1) The composition of intestinal flora in the population 1-5 years after cholecystectomy has changed. (2) The relative abundance of short-chain fatty acid-producing bacteria and probiotics in the population 1-5 years after cholecystectomy decreased, while the relative abundance of pathogenic bacteria increased.

Objective: To analyze the related indexes of vaginal microecology that affect the infection and outcome of high-risk human papillomavirus (HR-HPV), and to explore the correlation between HR-HPV and vaginal microecology, so as to provide the corresponding basis for the diagnosis and treatment of HR-HPV infection. Methods: Retrospective analysis of patients who underwent HR-HPV and vaginal secretion tests at the First Affiliated Hospital of Baotou Medical College from September 2021 to March 2023. According to the inclusion criteria, 1 052 cases of HR-HPV infection were selected, while 526 cases in the positive group and 526 cases in the negative group, and 131 cases in the returned portion: 76 cases in the returned group and 55 cases in the persistently infected group. The differences in the vaginal microecology of the above patients were compared between the groups. Results: (1) Age was correlated with HR-HPV infection and regression (P＜0.05). (2)The proportion of abnormal vaginal cleanliness, bacterial vaginitis (BV), pH＞4.5, H₂O₂(+),leukocyte esterase (LE) (+),and sialidase (SNA) (+)in the HR-HPV positive group was higher than that in HR-HPV negative group (P＜0.05); abnormal cleanliness, and SNA(+) were the independent risk factors for HR-HPV infection (P＜0.05). (3)The proportion of abnormal flora density, abnormal diversity, abnormal cleanliness, BV, pH＞4.5, H₂O₂(+), LE(+), SNA(+) in persistently infected group was higher than that of the regression group (P＜0.05); abnormal density, pH＞4.5, H₂O₂(+), LE(+) were the independent risk factors for the persistently infected of HR-HPV (P＜0.05); there was no change in the comparison of vaginal cleanliness before and after the regression of HR-HPV (P＞0.05). Conclusion: Vaginal microecological imbalance is associated with HR-HPV infection and persistence. Bacterial vaginitis, elevated pH value and abnormal H₂O₂ are risk factors for HR-HPV infection and persistence.

急性胰腺炎(acute pancreatitis, AP)是一种严重的消化系统疾病,其发生和发展与多种因素相关。近年来,肠道菌群失衡被认为是影响AP进程的重要因素。研究表明,AP患者的肠道菌群多样性减少、有益菌与致病菌比例失衡、特定菌群丰度的变化等,均通过影响肠道屏障功能和激发炎症反应影响AP进程。肠黏膜屏障在维持机体免疫防御系统完整性和正常生理功能方面起着至关重要的作用。肠道菌群失衡导致肠黏膜屏障功能受损,使肠道通透性增加,细菌发生易位,引发全身炎症反应综合征甚至多器官功能衰竭,加重胰腺炎病情,影响患者预后。此外,肠道菌群失衡还导致代谢产物的变化,如短链脂肪酸(short-chain fatty acids, SCFAs)和胆汁酸等,这些变化对胰腺功能和AP的发展有着重要影响。因此,维持肠道菌群平衡可能对AP的治疗和预后具有重要意义。笔者寻找对AP至关重要的特定菌株或参与AP的其他代谢产物,通过调节AP患者肠道菌群作为治疗AP的一种有效方法,深入研究肠道菌群与AP之间的相互作用,不仅有助于揭示AP的病理机制,还可能为AP的预防和治疗提供新的策略和方法。

Objective: To investigate the relationship between the activity and gene polymorphism of coagulation factor Ⅻ and the risk of recurrent spontaneous abortion (RSA) in Baotou Han population. Methods: RSA patients and healthy pregnant women were selected as the research objects. The activity of coagulation factor Ⅻ was measured. The genotype composition of FⅫ gene single nucleotide polymorphism (SNP) rs1801020 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results of the two groups were analyzed to determine the correlation between FⅫ gene SNP rs1801020 and RSA under different genetic models. Results: There were significant differences in the genotype and allele frequency distribution of SNP rs1801020 in FⅫ gene between the two groups (P＜0.05); the frequency of T allele in RSA group was lower than that in control group (χ²=8.756, OR=2.043, 95%CI: 1.267-3.295,P<0.05); the frequency of CT genotype in RSA group was higher than that in control group, while the frequency of TT genotype was lower than that in control group, and the difference was statistically significant (χ²=9.905, OR=2.611, 95%CI: 1.447-4.713,P＜0.05); FⅫ activity in RSA patients was different among rs1801020 genotypes, and the order of activity was CC, CT and TT. FⅫ SNP rs1801020 was associated with the risk of RSA under codominant, dominant and overdominant models (OR=2.611, 2.636, 2.470, P＜0.05); compared with TT genotype, CT and CT+CC genotypes could increase the risk of RSA; compared with CC+TT genotype, CT genotype could increase the risk of RSA. Conclusion: The difference in the distribution of FⅫ rs1801020 between the two groups may be related to the risk of RSA in Baotou Han women. Compared with TT genotype, mutant heterozygous CT genotype may increase the risk of RSA, the T allele of rs1801020 locus of FⅫ gene can cause the decrease of FⅫ activity.

Objective: To systematically evaluate the efficacy and adverse reactions of epirubicin (EPI) and mitomycin (MMC) in patients with non-muscle invasive bladder cancer (NMIBC) after perfusion chemotherapy. Methods: CNKI, Wanfang, VIP, SinoMed, PubMed, Embase, The Cochrance Library and Web of science were searched for Chinese and English randomized controlled trials of epirubicin and mitomycin in the treatment of NMIBC from the establishment of the database to January 2023. RevMan 5.3 software was used to perform a meta-analysis of postoperative tumor recurrence rate, total incidence of adverse reactions, incidence of bladder irritation, liver and kidney function damage, and hematuria in the EPI group and the MMC group. Results: There were 16 studies that met the inclusion and exclusion criteria, with a total of 1244 patients, while 626 patients in the EPI group and 618 patients in the MMC group. The results of meta-analysis showed that the postoperative tumor recurrence rate [OR=0.55,95%CI(0.41, 0.73), P＜0.01], the incidence of total adverse reactions [OR=0.56,95%CI(0.38, 0.82), P=0.003], the incidence of bladder irritation [OR=0.54, 95%CI(0.37, 0.77), P=0.0007], the incidence of hematuria [OR=0.53,95%CI(0.33, 0.85), P=0.009], and the incidence of liver and kidney dysfunction [OR=0.26, 95%CI(0.10, 0.69), P=0.007] in the EPI group were significantly lower than those in the MMC group (P＜0.05). Conclusion: EPI is superior to MMC in terms of therapeutic effect and total incidence of adverse reactions. EPI bladder perfusion is recommended to prevent tumor recurrence and improve the prognosis of patients after bladder cancer surgery.

Objective: To analyze the proportion of SPP1⁺ macrophages in peripheral blood of patients with connective tissue disease complicated with interstitial lung disease (CTD-ILD) and the expression level of its expression product chemokine 18 (CCL18) in serum of patients with CTD-ILD and its clinical significance. Methods: Twenty patients with connective tissue disease (CTD) and 20 healthy people were selected as the control group, and 35 patients with newly diagnosed CTD-ILD were selected as the experimental group. The differences in the expression levels of SPP1⁺ macrophages and CCL18 in the experimental group and the control group were compared, and the correlation with type I collagen (COL1A1), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) was analyzed. Results: Compared with the control group, the proportion of SPP1⁺ macrophages in peripheral blood mononuclear cells of CTD-ILD patients was significantly increased (P＜0.05), and the content of CCL18 in serum was also significantly increased (P＜0.05). At the same time, SPP1⁺ macrophages and CCL18 were positively correlated with ESR, CRP, COL1A1 and lung imaging score (HRCT score) (P＜0.05), and negatively correlated with the percentage of forced vital capacity (FVC%) and the percentage of carbon monoxide diffusion (DLCO%) (P＜0.05). The area under the curve (AUC) of SPP1⁺ macrophages and CCL18 were 0.732 and 0.939, respectively. The AUC of the combined detection of SPP1⁺ macrophages and CCL18 was 0.939, the sensitivity was 93.5%, the specificity was 83.3%, and the Youden index was 0.768. Conclusion: SPP1⁺ macrophages and CCL18 are poor prognostic factors in patients with CTD-ILD. The combined detection of SPP1⁺ macrophages and CCL18 is of great significance for the prognosis of the disease.

Objective: To investigate the active ingredients and mechanism of Sophora alopecuroides L. in the treatment of alcoholic liver disease (ALD) based on network pharmacology and molecular docking techniques. Methods: The active ingredients in Sophora alopecuroides L. were screened by Herb platform, and the corresponding targets of active ingredients were searched in SIB database. The targets of alcoholic liver disease were obtained from NCBI and GeneCards databases. The mapping tool Venny 2.1 was used for intersection comparison to obtain common targets. The protein interaction analysis and visual presentation were performed on the String platform. The DAVID database was used for GO function and KEGG pathway enrichment analysis. Finally, AutoDock and PyMOL software were used for molecular docking. Results: Eight active ingredients and 97 effective targets were identified by network pharmacology. The mechanism of action might be related to cancer pathway, lipid and atherosclerosis, T cell receptor signaling pathway and VEGF signaling pathway, and the main targets involved were HSP90AA1, AKT1, FYN and AKT2. Molecular docking results showed that matrine, sophocarpine and sophoramine had good binding force with HSP90AA1, FYN and AKT1. Conclusion: Matrine, sophocarpine and sophoramine in Sophora alopecuroides L. can ameliorate ALD by inhibiting inflammatory response or apoptosis through multi-target and multi-pathway interactions.

Objective: To analyze the clinicopathological features and laboratory characteristics of patients with dyslipidemia and liver dysfunction. Methods: The clinical data of 135 patients with liver dysfunction who underwent physical examination in the hospital from May 2019 to March 2022 were retrospectively analyzed. And they were divided into dyslipidemia group (n=60) and non-dyslipidemia group (n=75) according to whether accompanied by dyslipidemia. All patients underwent B-ultrasound examination, and the clinical data, clinicopathological characteristics, liver function indexes, lipid metabolism indexes and inflammatory indexes of were compared between the two groups. Results: There was no significant difference in gender and age between the two groups (P＞0.05). The proportion of liver and abdominal pain, the proportion of ascites and the incidence of total complications in the dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). The proportion of liver capsule thickening, echo enhancement, liver density decrease and liver margin roughness in dyslipidemia group was higher than that in non-dyslipidemia group (P＜0.05). The levels of total bilirubin (TBIL), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). The level of high density lipoprotein cholesterol (HDL-C) in dyslipidemia group was lower than that in non-dyslipidemia group (P＜0.05), and the levels of triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). The levels of serum C-reactive protein (CRP) and interleukin-6 (IL-6) in dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). Conclusion: The clinicopathological features of patients with liver dysfunction and dyslipidemia are mainly characterized by liver dysfunction, dyslipidemia, liver ascites, abdominal pain, high incidence of complications, decreased liver density, enhanced echo, rough liver margin, and increased inflammatory response.

Objective: To observe the effects of ribokinase (Rbks) gene knockout on phenotype and glucose and lipid metabolism in C57BL/6J mice. Methods: 12 male 8-week-old wide type (WT) and Rbks gene knockout (KO) C57BL/6J mice were fed for 10 weeks. The changes of appearance, body weight, food intake, water intake and fasting blood glucose were observed regularly. At the end of the observation period, the contents of triglyceride and cholesterol were measured. Results: After being fed normally for 10 weeks, compared with WT mice, KO mice had no significant difference in appearance and body weight(P＞0.05), but food intake and water intake decreased, fasting blood glucose level increased significantly(P＜0.05), serum triglyceride content increased and total cholesterol content decreased (all P＜0.05). Conclusion: Although Rbks gene knockout has no effect on the shape and weight of mice, it will affect the balance of glucose and lipid metabolism in mice.

Objective: To study the correlation between lipoprotein a, ApoE gene polymorphism and cardiac valve calcification, and to explore the role of genotype and lipoprotein a in the disease process. Methods: A total of 412 patients admitted to the Department of Cardiology from July 2021 to December 2022 were selected. The results of lipoprotein a and other blood lipids, ApoE genotype, echocardiography and carotid intima-media thickness (IMT) were collected, and the clinical data such as blood pressure were recorded. At the same time, the history of arrhythmia, heart failure, valvular heart disease and ACS were recorded. The correlation between ApoE genotype and blood lipid, cardiac valve calcification was analyzed. Results: The contents of TC, TG, lipoprotein a and LDL-C in the baseline data of the three groups were statistically different. Compared with the ε3 group, the contents of lipoprotein a and LDL-C in the ε2 group were lower, while those in the ε4 group were higher. Univariate analysis showed that age, HDL-C, TG, lipoprotein a, LDL-C, IMT, history of arrhythmia, history of heart failure, history of valvular heart disease were associated with valvular calcification. Binary logistic regression analysis showed that age, lipoprotein a, IMT and history of arrhythmia were independent risk factors for cardiac valve calcification. There was a correlation between lipoprotein a and cardiac valve calcification in the ε2 and ε3 subgroups, but there was no correlation in ε4 subgroup. The area under the curve for the combined diagnosis of IMT and lipoprotein a to predict cardiac valve calcification was 0.760 (P＜0.001). Conclusion: ApoE genotype does not directly affect the occurrence of calcification, but indirectly affects the disease by changing blood lipids. ε4 may be related to the increase of lipoprotein a and LDL-C. ε2 may be related to the decrease of lipoprotein a and LDL-C. Lipoprotein a may be related to the content of LDL-C.

Objective: To analyze the epidemiological characteristics, etiological surveillance, and clustering epidemic of hand-foot-mouth disease (HFMD) in Jinniu district of Chengdu from 2017 to 2023, so as to provide basis for the prevention and control strategy of HFMD. Methods: Descriptive epidemiological research methods were used to analyze the HFMD surveillance data in Jinniu district of Chengdu, from 2017 to 2023. Results: From 2017 to 2023, a total 12 960 cases of HFMD were reported in Jinniu district, with an annual incidence rate of 64.05/100 000 to 305.95/100 000; except for a few years, the number of began to increase in May each year, with the overall seasonal epidemic peaks occurring from May to July and from September to November. The male-to-female ratio of cases was 1.4∶1; the cases were mainly concentrated in children aged 0-＜6 years, accounting for 90.75% of the total number of cases; the most cases were scattered children, followed by children in kindergartens, accounting for 92.93% of the reported cases. The results of enterovirus monitoring showed that other enteroviruses, CVA16, and CVA6 were the main pathogens accounting for 59.75%, 15.56%, and 14.57%, respectively. The clustering epidemic was mainly concentrated from to July and from September to November, mainly occurring in kindergartens, accounting for 96.08% of the total; events with 2-5 cases were the most common, accounting for 93.56%. Conclusion: The prevention and control of HFMD should focus on young and scattered children, a particular emphasis on the changing dominant strains causing the disease. Outbreaks in Jinniu district mainly occurred in kindergartens, especially from May to and from September to November, during which targeted measures should be taken to reduce the occurrence and spread of the epidemic.

Objective: This article evaluates the biological safety of silver and stainless steel needles,which are used in Mongolian medicine.It provides scientific and technical support for their development and promotion. Methods: The experimental method followed the series of standards for biological evaluation of medical devices.Among them,Extraction standards for silver needle and stainless steel needle extracts followed the《Biological evaluation of medical devices - Part 12: Sample preparation and reference materials》(GB/T 16886.12-2023),Reference for extra-dermal and subcutaneous sensitization experiments followed the 《Biological evaluation of medical devices - Part 10: Irritation and skin sensitization tests》(GB/T 16886.10-2017),Blood compatibility test followed the《Biological evaluation of medical devices - Part 4: Selection of blood interaction tests》(GB/T 16886.4-2022),In this study, dermal and subcutaneous sensitization tests as well as blood compatibility tests were adopted to evaluate the biosafety of silver needles and stainless steel needles respectively.Male SD rats were used as experimental animals.The experimental needles were silver and stainless steel needles.The experimental solvent was needle extract and physiological saline.The general and local indicators,and the blood compatibility indicators of rats were observed in the experiment. Results: Silver and stainless steel needles did not cause obvious allergic reactions in rats in extracutaneous and subcutaneous sensitisation experiments,and did not affect the general and local indexes of rats.Silver and stainless steel needles did not cause any abnormalities such as blood coagulation,haemolysis and platelet aggregation in rats in the blood compatibility test. Conclusion: Silver and stainless steel needles are biocompatible and biologically effective,and have no significant adverse effects on the human body.They are suitable for Mongolian medical processes.

Objective: To investigate the role of logistic prediction model based on two-dimensional ultrasound image features to assess the risk of BRAF V600E mutation in TI-RADS 4a thyroid nodules. Methods: Patients with TI-RADS category 4a thyroid nodules who underwent post-puncture BRAF V600E gene testing at Yijishan Hospital, Wannan Medical College from January 2021 to September 2021 were collected, and data on ultrasound, pathology and gender and age of selected patients were obtained for retrospective analysis. The patients were divided into the BRAF V600E positive group(n=130)and BRAF V600E negative group(n=104)according to the gene test results. Multiple logistic regression models of two-dimensional ultrasound were constructed based on ultrasound image characteristics. The predictive efficacies of the models were assessed by ROC(receiver operating characteristic)curves and calibration curves, and the area under curve AUC was compared between the models using the Delong test. Results: The AUC for the training set obtained from the logistic echo model was 0.885 5(95%CI: 0.833 8-0.937 2), with specificity, sensitivity and accuracy of 67.0%, 96.0% and 83.0% respectively. The AUC of the validation set was 0.859 3(95%CI: 0.771 9-0.946 7), with specificity, sensitivity and accuracy of 79.0%, 84.0% and 82.0% respectively. Delong test results showed no statistically significant difference in AUC between the training and validation set models(P＞0.05). good clinical efficacy of the prediction models(total point 0.9)was showed by column plots. The calibration curve showed high agreement between the actual and predicted probabilities(C-index 0.9). Conclusion: Logistic regression models constructed based on 2D ultrasound image features can be used as a simple and non-invasive quantitative tool to predict the risk of BRAF V600E mutation in TI-RADS 4a thyroid nodules.

Objective: To observe the effect of berberine (BBR) on doxorubicin (DOX)-induced myocardial injury and its protective effect, and to further explore whether BBR can affect DOX-induced myocardial injury by regulating Wnt/β-catenin pathway. Methods: The rat myocardial cell line H9C2 was used as the research object and divided into Control group, model group, BBR group and LiCl agonist group. The cells in the control group were not treated. The DOX-induced myocardial injury model was established in the model group, BBR group and LiCl agonist group. The BBR group was intervened by BBR, and the LiCl agonist group was intervened by LiCl. CCK-8 method was used to detect the viability of cardiomyocytes. Flow cytometry was used to detect apoptosis. Western blot was used to detect the expression of Wnt/β-catenin pathway protein β-catenin, apoptosis-related proteins Bcl-2 and Bax. Results: (1)CCK-8 method : The cell viability of the model group was lower than that of the Control group (P＜0.05), while the cell viability of the BBR group and the LiCl agonist group was higher than that of the model group (P＜0.05). (2)Flow cytometry: The apoptosis rate of the model group was higher than that of the control group (P＜0.05). The apoptosis rate of BBR group and LiCl agonist group was lower than that of model group (P＜0.05). (3)Western blot: Compared with the Control group, the expression of Bax protein in the model group increased (P＜0.05), the expression of Bcl-2 and β-catenin protein decreased (P＜0.05), and the ratio of Bax/Bcl-2 also increased (P＜0.05). Compared with the model group, the expression of Bax protein in BBR group and LiCl agonist group decreased (P＜0.05), the expression of Bcl-2 protein increased (P＜0.05), the expression of β-catenin protein increased (P＜0.05), and the ratio of Bax/Bcl-2 decreased (P＜0.05). Conclusion: BBR may play a protective role in cardiomyocytes by promoting the expression of β-catenin in damaged cardiomyocytes, up-regulating the activation of Wnt/β-catenin signaling pathway and inhibiting cardiomyocyte apoptosis.

Objective: To investigate the mechanism of Banxia Baizhu Tianma Decoction (BBTD) on ischemic stroke. Methods: A total of 90 SD rats (245-280 g) were selected to prepare MCAO animal model by referring to Zea-longa's modified suture method. The cerebral infarction area of MCAO model animals was detected by TTC staining. The protein expression level of IL-6 in ischemic penumbra of ischemic brain tissue was detected by WB method, and the mRNA expression level of IL-6 in ischemic penumbra of ischemic brain tissue was detected by RT-PCR method. Results: Compared with the sham operation group, the percentage of cerebral infarction area in the model group increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the percentage of cerebral infarction area in BBTD and nimodipine treatment groups decreased to varying degrees, and the difference was statistically significant (P＜0.05). Compared with the sham operation group, the protein expression level of inflammatory factor IL-6 in the model group was significantly increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the protein expression level of inflammatory factor IL-6 in the BBTD and nimodipine treatment groups was decreased to varying degrees, and the difference was statistically significant (P＜0.05). Compared with the sham operation group, the mRNA expression level of inflammatory factor IL-6 in the model group was significantly increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the mRNA expression level of inflammatory factor IL-6 in BBTD and nimodipine treatment groups was decreased to varying degrees, and the difference was statistically significant (P＜0.05). Conclusion: Classic Banxia Baizhu Tianma Decoction can effectively alleviate ischemic brain injury in stroke events, which may be related to the inflammatory response mediated by IL-6.

Objective: To investigate the impact of Mongolian medicine saorilao-4 (SRL-4) on miRNA and core genes involved in the gene regulatory network of lung tissue in rats with pulmonary fibrosis (PF). Methods: Rats were randomly divided into four groups: blank control group (CON), model group (MOD), positive drug control group, and SRL-4 group. Except for the CON group, rats in the other groups were administered with bleomycin via intratracheal injection to establish a pulmonary fibrosis model. The total RNA was extracted from rat lung tissue for transcriptome sequencing. The differentially expressed miRNA (DEM) in each group was screened by the difference analysis software edge R. The differentially expressed gene (DEG) of DEM was predicted by miRanda. The GO and KEGG were used to analyze the biological function enrichment of DEG. Cytoscape was used to construct the target gene regulatory network and screen the core genes. Results: Compared with CON, MOD selected 16 DEMs. Compared with MOD, SRL-4 screened 10 DEMs and regulated 63 052 target genes. GO analysis showed that the DEGs of SRL-4 and MOD were enriched in 52 GO entries. KEGG analysis showed that DEG was enriched in 182 signaling pathways, among which the number of genes related to purine metabolic pathway was the highest. By constructing a gene regulatory network, six core genes were screened, namely Spata25, Sultan1a1, Mpv17i, Cryba4, Jakmip3 and Fkbp5. Conclusion: By constructing a miRNA-Target regulatory network between SRL-4 and MOD, we identified six hub genes that are considered key molecules in the gene regulatory network for the treatment of PF. The improvement effect of SRL-4 on PF may be related to miR-433-3p, novel_202, miR-150-3p, and these 6 core genes. The purine metabolism-related signaling pathway may be a critical target and essential pathway for the treatment of PF.

Objective: To observe the effect of quercetin on esophageal mucosal barrier function in rats with gastroesophageal reflux and explore its mechanism. Methods: Forty SD rats were randomly divided into blank control group, model group, quercetin administration group and positive control group. In addition to the blank control group, the rest of the rat model of reflux esophagitis was established. The quercetin administration group was given quercetin 50 mg/kg, the positive control group was given omeprazole 1.52 mg/kg, hydrotalcite 56.89 mg/kg, and domperidone 1.14 mg/kg. The blank control group was given an equal volume of normal saline once a day for 14 consecutive days. After the last administration, the rats were anesthetized and the blood was taken from the abdominal aorta, and the esophageal tissue samples were sacrificed. Then the pathological staining of esophageal tissue samples was performed, and the contents of TNF-α and IL-6 were detected by LISA, and the expression of IL-6 and TNF-α was detected by B. Results: Compared with the blank control group, the esophageal mucosa of the model group had obvious pathological changes, such as white and rough mucosa, defect, squamous epithelial hyperplasia, etc. Compared with the model group, the gastroesophageal histopathological changes in the quercetin treatment group were reduced. Compared with the blank control group, the contents of IL-6 and TNF-α in the model group were significantly increased (P＜0.05). Compared with the model group, the contents of IL-6 and TNF-α in the administration group and the positive control group were significantly decreased (P＜0.05). WB results showed that compared with the model group, the expression of TNF-α and IL-6 in the administration group and the positive control group was significantly decreased (P＜0.01). Conclusion: Quercetin may play a protective role by reducing inflammatory response and promoting gastroesophageal mucosal repair.

Objective: To investigate the protective effect of ursolic acid (UA) on alcoholic liver damage (ALD) mice by observing the expression of bile salt export pump (BSEP) and sodium taurocholate cotransporting polypeptide (NTCP) in mice after UA intervention. Methods: Eighteen 10-week-old SPF C57BL/6J mice were randomly divided into three groups (normal control group, alcohol model group, and UA+alcohol group). The mice were treated with intragastric administration for 7 weeks. The changes of body weight were recorded. The pathological changes of liver tissue were observed by HE staining. The serum total bile acid (TBA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected by microplate method. The expression of BSEP and NTCP protein in liver tissue was detected by Western blotting. Results: The average body weight of mice in the alcohol group was significantly lower than that in the normal control group (P＜0.05). The structure of hepatic lobules in the normal control group was intact and there was no inflammatory cell infiltration, while the structure of hepatic lobules in the alcohol model group was blurred, accompanied by obvious steatosis, and the NAS score was significantly increased (P＜0.05). Compared with the alcohol model group, after UA intervention, the damage of hepatic lobule structure in mice was improved, the number of fat vacuoles and inflammatory cells in hepatocytes was reduced, and the NAS score was significantly reduced (P＜0.05). In addition, compared with the normal control group, the liver index of the alcohol model group was significantly increased (P＜0.05), while the liver index of the UA+alcohol group was significantly lower than that of the alcohol model group (P＜0.05). At the same time, after UA intervention, the increase of TBA, AST and ALT induced by alcohol intake was significantly inhibited (P＜0.05). The results of Western blotting showed that compared with the normal control group, the expression levels of BSEP and NTCP in the liver tissue of the alcohol model group were significantly decreased (P＜0.05). After UA intervention, the expression levels of transporter BSEP and NTCP protein in UA+alcohol group were significantly increased (P＜0.05). Conclusion: UA supplementation can significantly improve liver damage caused by alcohol, and the mechanism may be related to the up-regulation of BSEP and NTCP protein expression, thereby regulating bile acid homeostasis.

Objective: To explore whether triptolide can alleviate the disease by reducing the infiltration of neutrophils in the joint cavity of collagen-induced arthritis (CIA) mice, and to analyze whether triptolide can reduce the formation of neutrophil extracellular capture networks (NETs) in patients with rheumatoid arthritis (RA) and its mechanism of action, so as to provide theoretical support for the clinical application of tripterygium glycosides in RA. Methods: The core targets of Tripterygium wilfordii intervention in RA disease were explored by using the public database of network medicine. After the CIA model was constructed using DBA1 mice, triptolide was given for 60 days. The hind limbs of the mice were collected for HE staining and the degree of inflammatory cell infiltration in the joint cavity of the mice was observed. Neutrophils in peripheral blood of RA patients were collected and stimulated with triptolide for 2 h. The formation of NETs was observed by immunofluorescence staining. Treatment with triptolide in RA group and triptolide could alleviate joint swelling in CIA mice; triptolide could also inhibit the formation of NETs in peripheral blood neutrophils of RA patients. Transcriptome sequencing was performed on neutrophils in RA group and Tripterygium wilfordii treatment group. Results: Through network pharmacology analysis, PPI protein interaction was performed on the locked overlapping genes, and KEGG and GO analysis were performed. It was found that IL-17, TNF signaling pathway and apoptosis-related pathway were closely related to RA disease. In vivo experiments showed that 389 differential genes were screened from the transcriptome sequencing results of the triptolide treatment group. The GO and KEGG pathways were mainly enriched in JAK-STAT, TNF, and PI3K-Akt signaling pathways. Conclusion: Triptolide can alleviate joint swelling in CIA mice by down-regulating neutrophil infiltration in the joint cavity of CIA mice, and triptolide can reduce the formation of NETs in peripheral blood of RA.This phenomenon may be related to JAK-STAT signaling pathway, TNF signaling pathway, PI3K-Akt and other signaling pathways.

随着中药病理学研究的不断发展,中药在治疗变应性鼻炎方面的理论日益充实且成效显著。近年来,变应性鼻炎的机制研究取得了长足的进展,其中自噬及其通路更成为该领域的热门研究方向。本文通过对细胞自噬发挥治疗作用的中药单体、方剂及其调控通路进行总结归纳与系统分析,以期明确其中的生物学规律和特点,为后期更深入的研究提供参考。