Objective: To analyze the proportion of SPP1⁺ macrophages in peripheral blood of patients with connective tissue disease complicated with interstitial lung disease (CTD-ILD) and the expression level of its expression product chemokine 18 (CCL18) in serum of patients with CTD-ILD and its clinical significance. Methods: Twenty patients with connective tissue disease (CTD) and 20 healthy people were selected as the control group, and 35 patients with newly diagnosed CTD-ILD were selected as the experimental group. The differences in the expression levels of SPP1⁺ macrophages and CCL18 in the experimental group and the control group were compared, and the correlation with type I collagen (COL1A1), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) was analyzed. Results: Compared with the control group, the proportion of SPP1⁺ macrophages in peripheral blood mononuclear cells of CTD-ILD patients was significantly increased (P＜0.05), and the content of CCL18 in serum was also significantly increased (P＜0.05). At the same time, SPP1⁺ macrophages and CCL18 were positively correlated with ESR, CRP, COL1A1 and lung imaging score (HRCT score) (P＜0.05), and negatively correlated with the percentage of forced vital capacity (FVC%) and the percentage of carbon monoxide diffusion (DLCO%) (P＜0.05). The area under the curve (AUC) of SPP1⁺ macrophages and CCL18 were 0.732 and 0.939, respectively. The AUC of the combined detection of SPP1⁺ macrophages and CCL18 was 0.939, the sensitivity was 93.5%, the specificity was 83.3%, and the Youden index was 0.768. Conclusion: SPP1⁺ macrophages and CCL18 are poor prognostic factors in patients with CTD-ILD. The combined detection of SPP1⁺ macrophages and CCL18 is of great significance for the prognosis of the disease.

Objective: To optimize and enhance the quality standard of Yurijle-13 pill, a Mongolian medicinal preparation. Methods: :Microscopic identification was carried out on the powder of the formula encompassing Aucklandia lappa Decne, Gardenia jasminoides Ellis, Chrysanthemum morifolium Ramat, Punica granatum L, and Piper longum L in Yurijle-13 pill. Thin Layer Chromatography (TLC) was established for the identification of Aucklandia lappa Decne, Gardenia jasminoides Ellis, and Salvia miltiorrhiza Bge in Yurijle-13 pill. High Performance Liquid Chromatography (HPLC) was developed to concurrently determine the contents of the principal ingredient, Costunolide (CL), and Dehydrocostus lactone (DL) in the Aucklandia lappa Decne of Yurijle-13 pill by using a SinoPak SP analytical column (4.6 mm×250 mm, 5 μm), with a mixture of methanol and water (6535) as the mobile phase, a flow rate of 1.0 mL/min, a column temperature of 30 ℃, and a detection wavelength of 225 nm. Results: :The microscopic characteristics of the powder of Aucklandia lappa Decne, Gardenia jasminoides Ellis, Chrysanthemum morifolium Ramat, Punica granatum L, and Piper longum L in Yurijle-13 pill were distinct. Aucklandia lappa Decne, Gardenia jasminoides Ellis, and Salvia miltiorrhiza Bge demonstrated strong specificity in TLC qualitative identification. The linear regression equation for CL was y=315.83x-3.9247 (within the concentration range of 0.0051 to 0.2504 mg/mL), and that for DL was y=276.65x-5.5702 (within the concentration range of 0.0497 to 0.2494 mg/mL). The RSDs for the tests of precision, stability, and reproducibility were 1.13% and 1.17%, 3.43% and 3.04%, and 0.65% and 0.26%, respectively, and the average recovery rates were within the specified range (85.0% to 110.0%). Conclusion: :Compared with the Inner Mongolia Autonomous Region hospital preparation approved, the method for identification and content determination of Yourile-13 pill established in this study not only increase the types of identified medicinal materials and the main components of the drug, but also has the advantages of convenient operation, strong specificity and good repeatability, which is suitable for the quality control of Yourile-13 pill.

家庭参与式护理模式(family integrated care, FICare)具备促进早产儿成长发育、减轻早产儿父母负面情绪以及提升早产儿父母育儿照护能力等优势。本文详细阐述了FICare的起源、定义、特点、应用状况、干预内容以及实施方法,并针对我国新生儿监护病房(neonatal intensive care unit, NICU)所面临的挑战,提出了相应的建议与展望,旨在为国内早产儿护理提供参考依据。

Objective: To analyze the epidemiological characteristics, etiological surveillance, and clustering epidemic of hand-foot-mouth disease (HFMD) in Jinniu district of Chengdu from 2017 to 2023, so as to provide basis for the prevention and control strategy of HFMD. Methods: Descriptive epidemiological research methods were used to analyze the HFMD surveillance data in Jinniu district of Chengdu, from 2017 to 2023. Results: From 2017 to 2023, a total 12 960 cases of HFMD were reported in Jinniu district, with an annual incidence rate of 64.05/100 000 to 305.95/100 000; except for a few years, the number of began to increase in May each year, with the overall seasonal epidemic peaks occurring from May to July and from September to November. The male-to-female ratio of cases was 1.4∶1; the cases were mainly concentrated in children aged 0-＜6 years, accounting for 90.75% of the total number of cases; the most cases were scattered children, followed by children in kindergartens, accounting for 92.93% of the reported cases. The results of enterovirus monitoring showed that other enteroviruses, CVA16, and CVA6 were the main pathogens accounting for 59.75%, 15.56%, and 14.57%, respectively. The clustering epidemic was mainly concentrated from to July and from September to November, mainly occurring in kindergartens, accounting for 96.08% of the total; events with 2-5 cases were the most common, accounting for 93.56%. Conclusion: The prevention and control of HFMD should focus on young and scattered children, a particular emphasis on the changing dominant strains causing the disease. Outbreaks in Jinniu district mainly occurred in kindergartens, especially from May to and from September to November, during which targeted measures should be taken to reduce the occurrence and spread of the epidemic.

Objective: This article evaluates the biological safety of silver and stainless steel needles,which are used in Mongolian medicine.It provides scientific and technical support for their development and promotion. Methods: The experimental method followed the series of standards for biological evaluation of medical devices.Among them,Extraction standards for silver needle and stainless steel needle extracts followed the《Biological evaluation of medical devices - Part 12: Sample preparation and reference materials》(GB/T 16886.12-2023),Reference for extra-dermal and subcutaneous sensitization experiments followed the 《Biological evaluation of medical devices - Part 10: Irritation and skin sensitization tests》(GB/T 16886.10-2017),Blood compatibility test followed the《Biological evaluation of medical devices - Part 4: Selection of blood interaction tests》(GB/T 16886.4-2022),In this study, dermal and subcutaneous sensitization tests as well as blood compatibility tests were adopted to evaluate the biosafety of silver needles and stainless steel needles respectively.Male SD rats were used as experimental animals.The experimental needles were silver and stainless steel needles.The experimental solvent was needle extract and physiological saline.The general and local indicators,and the blood compatibility indicators of rats were observed in the experiment. Results: Silver and stainless steel needles did not cause obvious allergic reactions in rats in extracutaneous and subcutaneous sensitisation experiments,and did not affect the general and local indexes of rats.Silver and stainless steel needles did not cause any abnormalities such as blood coagulation,haemolysis and platelet aggregation in rats in the blood compatibility test. Conclusion: Silver and stainless steel needles are biocompatible and biologically effective,and have no significant adverse effects on the human body.They are suitable for Mongolian medical processes.

Objective: To measure the quality of life of patients with ischemic stroke by using five-dimensional euro quality of life (EQ-5D) and stroke specific quality of life scale (SS-QOL), and to compare the differences between EQ-5D and SS-QOL. Methods: The EQ-5D and SS-QOL were used to evaluate the quality of life of 236 ischemic stroke patients in a tertiary hospital in Nantong city. Internal consistency reliability, known-group validity, Spearman's correlation, discriminant validity, and central tendency of the two scales were compared and analyzed. Results: The Cronbach's α coefficients of EQ-5D and SS-QOL were 0.826 and 0.948, respectively. EQ-5D and SS-QOL scores were different in different infarct hemispheres, NIHSS score, water swallow test grades, and other disease-related characteristics (P＜0.05). The two scales showed a significant overall correlation (r=0.773, P=0.000), but some dimensions were not correlated (P＞0.05). There were significant differences in EQ-5D utility value and EQ-VAS value among different SS-QOL scores (P＜0.01). The total score of SS-QOL was statistically significant in different dimensions and levels of EQ-5D (P＜0.01). The EQ-5D exhibited a ceiling effect (53.4%), with an overall poor central tendency. In contrast, the SS-QOL total score showed no ceiling effect and had a good overall central tendency. Conclusion: EQ-5D and SS-QOL have good reliability, validity and discrimination, which can be used to evaluate the quality of life of patients with ischemic stroke. EQ-5D has a ceiling effect and is suitable for rapid screening of moderate and severe patients. SS-QOL has no ceiling effect and is suitable for comprehensive evaluation of the dynamic changes of patients' quality of life.

Objective: To investigate the protective effect of ursolic acid (UA) on alcoholic liver damage (ALD) mice by observing the expression of bile salt export pump (BSEP) and sodium taurocholate cotransporting polypeptide (NTCP) in mice after UA intervention. Methods: Eighteen 10-week-old SPF C57BL/6J mice were randomly divided into three groups (normal control group, alcohol model group, and UA+alcohol group). The mice were treated with intragastric administration for 7 weeks. The changes of body weight were recorded. The pathological changes of liver tissue were observed by HE staining. The serum total bile acid (TBA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected by microplate method. The expression of BSEP and NTCP protein in liver tissue was detected by Western blotting. Results: The average body weight of mice in the alcohol group was significantly lower than that in the normal control group (P＜0.05). The structure of hepatic lobules in the normal control group was intact and there was no inflammatory cell infiltration, while the structure of hepatic lobules in the alcohol model group was blurred, accompanied by obvious steatosis, and the NAS score was significantly increased (P＜0.05). Compared with the alcohol model group, after UA intervention, the damage of hepatic lobule structure in mice was improved, the number of fat vacuoles and inflammatory cells in hepatocytes was reduced, and the NAS score was significantly reduced (P＜0.05). In addition, compared with the normal control group, the liver index of the alcohol model group was significantly increased (P＜0.05), while the liver index of the UA+alcohol group was significantly lower than that of the alcohol model group (P＜0.05). At the same time, after UA intervention, the increase of TBA, AST and ALT induced by alcohol intake was significantly inhibited (P＜0.05). The results of Western blotting showed that compared with the normal control group, the expression levels of BSEP and NTCP in the liver tissue of the alcohol model group were significantly decreased (P＜0.05). After UA intervention, the expression levels of transporter BSEP and NTCP protein in UA+alcohol group were significantly increased (P＜0.05). Conclusion: UA supplementation can significantly improve liver damage caused by alcohol, and the mechanism may be related to the up-regulation of BSEP and NTCP protein expression, thereby regulating bile acid homeostasis.

Objective: To explore the value of nucleic acid detection of pathogenic bacteria in lower respiratory tract in the diagnosis of lower respiratory tract infection. Methods: Patients who underwent nucleic acid testing and bacterial culture for lower respiratory pathogens from December 2023 to November 2024 were selected as subjects. The detection rates of respiratory pathogens by both methods were compared. Results: For six common pathogens causing lower respiratory infections, the positive detection rate of nucleic acid testing was significantly higher than that of conventional bacterial culture (69.68% vs.20.88%). For Pseudomonas aeruginosa, the two methods had good consistency in positive detection rates (Kappa=0.644, P＜0.001), while the consistency of detection rate of klebsiella pneumoniae between the two methods was general (Kappa=0.473, P＜0.001), and that of staphylococcus aureus was poor (Kappa=0.287, P＜0.001). The average turnaround time within the laboratory for nucleic acid testing was 4.68 hours, while that for conventional bacterial culture was 49.75 hours, the average turnaround time of nucleic acid detection in the laboratory was significantly lower than that of bacterial culture (P＜0.001). Conclusion: Nucleic acid detection of pathogenic bacteria in lower respiratory tract has more advantages in rapid identification of pathogenic bacteria in respiratory tract, with shorter laboratory turnaround time, can provide timely information on pathogenic bacteria for clinicians, and has higher clinical significance for patients with severe and rapid progress.

Objective: To observe the effect of Mongolian medicine total flavonoids of Dracocephalum moldavica L (TFDM) on the expression of microtubule-associated protein-2 (MAP-2) in the ischemic penumbra of rats after cerebral ischemia-reperfusion, and explore its role in neural plasticity. Methods: Constructing a middle cerebral artery occlusion reperfusion model using SD rats. The experiment was divided into sham surgery group (Sham), ischemia-reperfusion group (I/R), and TFDM administration group (TFDM). Samples were collected at four time points: 1, 3, 7, 14 days after surgery. The immunohistochemical staining was used to observe the expression of MAP-2 positive labeled cells and dendrites in the ischemic penumbra of the cortex, and Western blot was used to detect the expression of MAP-2 protein in the ischemic penumbra of the cortex. Results: The results of immunohistochemistry and Western Blot showed that the expression of MAP-2 in the ischemic penumbra of the TFDM group was significantly higher than that in the I/R group on the 3rd and 7th day after reperfusion (P＜0.05). Conclusion: TFDM may play a neuroprotective role by up-regulating the expression of MAP-2 protein, enhancing the regulation of neural plasticity in the ischemic penumbra, promoting dendritic growth and repairing damaged nerve structure.

Objective: To investigate and analyze the efficacy of high-flow nasal cannula oxygen therapy in the sequential treatment of mechanical ventilation weaning for pneumonia complicated with type I respiratory failure. Methods: A total of 70 patients with pneumonia and type I respiratory failure admitted from January 2023 to December 2024 were divided into experimental group and control group. All patients were treated with mechanical ventilation. The experimental group was treated with high-flow nasal cannula oxygen therapy, and the control group was treated with non-invasive positive pressure ventilation. The clinical efficacy, blood gas analysis indexes (before treatment, 12, 24 and 48 hours after treatment) and sputum traits, inflammation index levels (before treatment and 3 days after treatment), sequential treatment time, total hospitalization time and reintubation mechanical ventilation rate were compared between the two groups. Results: The total effective rate of clinical efficacy in the experimental group was higher than that in the control group (P＜0.05). There were no statistically significant differences in PaO₂, PaCO₂, and SaO₂ levels between the two groups before treatment and at 12, 24, and 48 hours after treatment (P＞0.05). There was no significant difference in PaO₂, PaCO₂ and SaO₂ levels between the two groups before treatment and 12,24 and 48 hours after treatment (P＞0.05). There was no difference in CRP and PCT levels between the two groups before treatment (P＞0.05). After 3 days of treatment, CRP and PCT in the experimental group were lower than those in the control group (P＜0.05). There was no significant difference in sputum traits between the two groups before treatment (P＞0.05). After 12, 24 and 48 hours of treatment, the sputum traits I of the experimental group were better than those of the control group (P＜0.05). The sequential treatment time and total hospitalization time of the experimental group were lower than those of the control group (P＜0.05). Conclusion: High-flow nasal cannula oxygen therapy can improve the clinical efficacy of patients with pneumonia complicated with type I respiratory failure after mechanical ventilation weaning, dilute sputum, improve oxygenation, reduce inflammation, improve patient tolerance, reduce the risk of re-intubation mechanical ventilation due to intolerance, shorten the treatment time, and promote its rehabilitation. It is worthy of application.

Objective: To analyze the expression level and the correlation with clinical data of type X collagen α 1 chain (COL10A1) in the serum of breast cancer patients, so as to evaluate its predictive value for breast cancer risk. Methods: GEPIA database was used to analyze the expression of COL10A1 in breast cancer patients. The expression of COL10A1 in the serum of 30 breast cancer patients was detected by PCR, and its relationship with the clinical data of patients was analyzed. ROC curve was used to analyze the clinical diagnostic value of COL10A1 in breast cancer. The potential targets of COL10A1-mediated breast cancer were obtained through the Genecards database and OMIM database, and they were input into the STRING database to obtain the TSV file of the protein-protein interaction (PPI) network. GO enrichment analysis and KEGG enrichment analysis were used to explore different functional categories and related signal transduction pathways. Results: Bioinformatics analysis and PCR results showed that COL10A1 was highly expressed in breast cancer, which was closely related to histological grade, TNM stage, distant lymph node metastasis and Her2. ROC curve showed that the area under the curve of COL10A1 was 0.690 0 (95%CI: 0.5567-0.8233). When the optimal cut-off value was 1.1026, the sensitivity and specificity of COL10A1 in the diagnosis of breast cancer were 60.0% and 68.9%, respectively. A total of 14 potential targets of COL10A1-mediated breast cancer were obtained by combining the Genecards database with the OMIM database. A total of 241 GO terms were enriched by GO analysis, including 9 cellular components, 48 biological processes, and 12 molecular functions. KEGG enrichment analysis enriched a total of 12 signal transduction pathways. Conclusion: The high expression of serum COL10A1 in breast cancer patients is closely related to clinicopathological indicators, and has certain clinical value for breast cancer risk assessment.

慢性阻塞性肺疾病(chronic obstructive pulmonary diseases, COPD)是一种进展性呼吸系统疾病,伴随长期的炎症反应。常用的治疗药物虽然能改善患者部分临床症状,但往往会面临药物依赖性和不良反应等诸多挑战,寻找相对安全、有效的治疗策略成为目前亟须解决的问题。天然来源的中药及其活性成分在抗炎和调节免疫功能等方面表现出较大的优势和潜力,为COPD的治疗带来了新的契机。因此,本文系统梳理了近年来关于中药复方、单体及其活性成分通过多种炎症相关信号通路干预COPD的研究进展,旨在为中药治疗COPD提供理论依据与研究思路。

Objective: To analyze the correlation between the levels of serum transcription activator factor 3 (ATF3), tumor necrosis factor receptor 1 (TNFR1), angiopoietin-like protein 4 (ANGPTL4) and the renal function, prognosis of patients with diabetic nephropathy. Methods: A total of 152 patients with diabetic nephropathy who were treated in the Second People's Hospital of Jiaozuo City from April 2022 to January 2024 were selected as the study group, and 152 patients with simple diabetes who were treated in the same period were selected as the control group. The levels of serum ATF3, TNFR1, ANGPTL4 and renal function indexes such as serum calcium, serum phosphorus, serum creatinine and glomerular filtration rate (eGFR) were compared between the two groups at admission, and their correlation was analyzed. The levels of serum ATF3, TNFR1 and ANGPTL4 in patients with different prognosis were compared at admission, and their correlation with prognosis and predictive value for prognosis were analyzed by partial regression. Results: Compared with the control group, the levels of ATF3, TNFR1, and ANGPTL4 in the study group were significantly higher at admission (P＜0.05). The levels of blood calcium and creatinine in the study group were higher than those in the control group, while the levels of blood phosphorus and eGFR were lower than those in the control group (P＜0.05). Serum ATF3, ANGPTL4, and TNFR1 were negatively correlated with blood phosphorus and eGFR, and positively correlated with blood calcium and creatinine levels (P＜0.05). In the study group, patients with poor prognosis had higher levels of ATF3, ANGPTL4, and TNFR1 on admission than patients with good prognosis (P＜0.05). Logistic regression analysis showed that serum ATF3, ANGPTL4 and TNFR1 were risk factors for poor prognosis of patients (P＜0.05). The efficacy of combined prediction of serum ATF3, TNFR1 and ANGPTL4 levels on admission in predicting poor prognosis of patients was 0.807, which was higher than that of each index alone (P＜0.05). Conclusion: The increase in serum levels of ATF3, TNFR1, and ANGPTL4 in patients with diabetic nephropathy is associated with their renal function. Clinical detection of these levels can help predict the prognosis of patients and provide effective reference for clinical diagnosis and treatment.

Objective: To identify the key genes related to the occurrence and development of lung adenocarcinoma by bioinformatics, and to explore its potential application value in the survival and prognosis of lung adenocarcinoma. Methods: The transcriptome data from the cancer genome atlas (TCGA) database were screened for differentially expressed genes between lung adenocarcinoma and normal tissues via R software. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) were subsequently performed to study the function and pathway enrichment of the identified DEGs. Weighted gene co-expression network analysis (WGCNA) was used to identify the key modules and the genes in key modules based on the lung adenocarcinoma dataset from TCGA database. Kaplan-Meier analysis and Cox proportional hazards regression model were used to analyze the predictors related to the prognosis of patients with lung adenocarcinoma. Results: A total of 1 749 DEGs based on the gene expression data from TCGA were screened. By WGCNA, there were six gene modules related to the clinical characteristics of patients with lung adenocarcinoma, of which the blue, brown, turquoise, yellow and green modules showed the highest positive correlation with the occurrence and development of lung adenocarcinoma (r=0.69, r=0.42, r=-0.65, r=-0.41, r=-0.30, P＜0.01). Ultimately, CDK1, DLGAP5 and PLK1 were identified by integrating the DEGs with key module genes of WGCNA and then the overexpression of CDK1, DLGAP5 and PLK1 was associated with a poor prognosis in patients with lung adenocarcinoma (P＜0.05). Conclusion: CDK1, DLGAP5 and PLK1 are associated with a poor prognosis of lung adenocarcinoma, which as potential therapeutic targets for improving the prognosis of lung adenocarcinoma.

Objective: To investigate the effects of oral nutrition supplementation 10 days before surgery on preoperative nutritional status, immune function and postoperative infectious complications in patients with oral cancer. Methods: A total of 40 elderly patients with preoperative oral cancer who were screened by Nutritional Risk Screening 2002 (NRS-2002) score were divided into observation group and control group according to random number table method, with 20 patients in each group. The two groups were given dynamic nutritional support according to the target demand of energy and protein after operation. The control group was given routine nutritional education before operation. The observation group was given Nengquansu combined with whey protein powder oral nutrition supplement on the basis of the control group. The changes of nutritional status indexes, immune function indexes and postoperative infectious complications before and after intervention were compared between the two groups. Results: After intervention, the levels of albumin and prealbumin in the observation group were significantly higher than those in the control group, and the differences were statistically significant (P＜0.05), while there was no significant difference in RBC, HB between the observation group and the control group (P＞0.05). After intervention, IgA, CD4 and CD4/CD8 of the observation group were significantly higher than those of the control group, while C-reactive protein of the observation group was significantly lower than that of the control group, the differences were statistically significant (P＜0.05). After intervention, there was no significant difference in IgG, IgM, CD3, CD8 and NK cells between the observation group and the control group (P＞0.05). After intervention, the proportion of the total number of complications in the observation group was significantly lower than that in the control group, and the difference was statistically significant (P＜0.05). Conclusion: The preoperative oral nutritional supplement of Nengquansu combined with whey protein powder can improve the preoperative nutritional status and immune function of patients with oral cancer, and reduce the incidence of postoperative infectious complications.

Objective: To analyze the infection rate of hepatitis E virus (HEV) in different populations, and to explore the difference of HEV co-infection in different populations, so as to provide reference for early prevention and treatment of HEV co-infection in different populations, and to reduce the harm caused by HEV infection. Methods: A total of 805 serum samples were collected according to the inclusion and exclusion criteria, including 200 serum samples from normal population, 298 serum samples from pregnant women, 281 serum samples from patients with chronic liver disease (156 samples of chronic hepatitis B, 24 samples of chronic hepatitis C, 101 samples of other chronic liver diseases), and 26 serum samples from HIV-infected patients. HEV-specific IgG antibodies were detected by ELISA (capture method). Results: The positive rate of anti-HEV-IgG in HIV infected group was higher than that in normal group (P＜0.05). The age of anti-HEV-IgG positive group in HIV infected group was older than that in negative group (P＜0.05). The age of anti-HEV-IgG positive group was older than that of negative group (P＜0.05). The positive rate of anti-HEV-IgG in chronic liver disease population was higher than that in normal population (P＜0.05). In the group over 40 years old, the positive rate of anti-HEV-IgG in chronic liver disease group was higher than that in normal population (P＜0.05). Conclusion: HIV-infected patients and patients with chronic liver disease are high-risk groups for HEV infection, and the HEV infection rate is significantly higher than that of the normal population, and the risk of co-infection with HEV increases with age. Therefore, it is necessary to study the infection rate of HEV co-infection in different populations, promote multidisciplinary cooperation, effectively screen HEV-infected populations, and provide reference for early prevention and rational treatment of HEV.

Objective: To observe the efficacy and safety of bidirectional subintimal angioplasty under fluoroscopy in the treatment of TASC Ⅱ C and D lesions of lower extremity arteriosclerosis obliterans (ASO). Methods: The clinical data of 22 patients with TASC Ⅱ grade C and D (24 limbs in total) ASO who underwent bidirectional subintimal angioplasty in the Department of Interventional Vascular, Hefei Second People's Hospital from June 2018 to June 2021 were collected. The success rate of operation, the incidence of complications in hospital and the improvement rate of ankle brachial index (ABI) after operation were statistically analyzed. Results: A total of 23 limbs were successfully operated, and 1 limb failed to open the occluded segment after repeated attempts of antegrade and retrograde arteries of the lower leg, with a success rate of 95.8%. The incidence of postoperative complications was 30.4%, and the mortality rate was 0.0%. The postoperative ABI was (0.79±0.16), which was better than(0.27±0.19) of the preoperative ABI (P＜0.05). The 6-month target vessel patency rate was 91.3% (21/23). There was no new amputation within 6 months. Conclusion: Bidirectional subintimal angioplasty is a safe and convenient endovascular treatment method. It is an effective remedy for long-segment and severe lower extremity arterial occlusive lesions that cannot be recanalized.

Objective: The endophytes with antibacterial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans are isolated and screened from Schisandra chinensis fruit, providing more screening sources for developing new clinical antibacterial drugs. Methods: The inhibitory activities of the endophytes against the three test pathogens were detected by the plate standoff method and Oxford cup method, and the data were statistically analyzed by SPSS Statistics 20.0 software, and the genera of the endophytes were further identified by morphological observation, physiological and biochemical characteristics, and molecular biology methods. Results: 25 endophytic strains were isolated, among which the fermentation broth of strain GF20 had strong antibacterial activity against the three tested pathogenic bacteria. After identification, GF20 was determined to be a bacterium of the genus Bacillus. Conclusion: Schisandra chinensis fruits are rich in endophytic bacteria, which have different antibacterial activities against common pathogenic bacteria and can potentially develop new antibacterial drugs.

Objective: To observe the expression and role of long non-coding RNA(LncRNA) potassium voltage-gated channel subfamily q member 1 overlapping transcription 1(KCNQ1OT1) in coronary atherosclerotic heart disease(CHD), and to explore whether it can become a potential biomarker for predicting acute myocardial infarction(AMI). Methods: A total of 240 patients were selected and divided into AMI group, unstable angina pectoris(UAP) group, stable angina pectoris(SAP) group and non-coronary heart disease control group, with 60 cases in each group. Venous blood was collected from the AMI group 6 hours after symptom onset, and blood samples were collected from the other three groups in the early morning of the next day after admission. The supernatant of the blood samples was extracted after centrifugation and frozen for RNA extraction. The basic clinical data, myocardial injury markers, lipid levels and other test results of the selected patients were recorded, and their history of hypertension and diabetes was noted. The expression levels of LncRNA in the peripheral blood of the patients were detected by real-time fluorescence quantitative PCR(RT-qPCR), and the ROC curve was drawn and the area under the curve was calculated to determine the diagnostic value of LncRNA. The correlation between LncRNA and some myocardial injury markers, N-terminal pro-B-type natriuretic peptide(NT-proBNP), and left ventricular ejection fraction(LVEF) was analyzed. Results: (1) The expression of LncRNA KCNQ1OT1 in the serum of AMI patients was significantly higher than that in the control group(P＜0.05).(2) The expression of LncRNA KCNQ1OT1 in the serum of AMI patients was positively correlated with the expression of cTnI, CK-MB and NT-proBNP, and negatively correlated with the expression of LVEF%.(3) The predictive ability of LncRNA KCNQ1OT1: AUC was 0.793(95% CI: 0.727 - 0.859), with a sensitivity of 60.2%, a specificity of 90.2%, and a cutoff value of 1.635. Conclusion: The highly expressed LncRNA KCNQ1OT1 in serum can serve as a biological marker for the diagnosis and prognosis assessment of AMI.

Objective: To investigate whether total glycosides from cornus officinalis (TGCO) can improve cognitive dysfunction caused by hypothyroidism via regulating cholinergic anti-inflammatory pathway (CAP). Methods: Sixty adult male SD rats were randomly divided into model group, blank control group, TGCO low-dose group (3 g/kg·d), TGCO medium-dose group (6 g/kg·d), TGCO high-dose group (12 g/kg·d) and positive control group (Euthyrox). The rat model of hypothyroidism was prepared by intragastric administration of 0.1% propylthiouracil solution for 30 days. After successful modeling, the corresponding doses of TGCO were given to each administration group for 21 days. The learning and cognitive function was tested by Morris water maze. The levels of serum thyroid hormones and related neurotransmitters were measured by ELISA. The expression of inflammatory factors, neurotransmitter synthase and related signaling pathway proteins in the brain were detected by Western blot. Results: Compared with the model group, the escape latency of each TGCO dose group was gradually shortened, the target quadrant residence time and the number of crossing the platform were gradually increased, and the effect of the high dose group was the most significant (P＜0.05). Compared with the model group, the levels of triiodothyronine (T3) and tetraiodothyronine (T4) in the serum of rats in the TGCO high-dose group were increased, and the levels of thyroid stimulating hormone (TSH) were decreased (P＜0.05). At the same time, the levels of acetylcholine (Ach) and dopamine (DA) in the serum of rats in the TGCO high-dose group were increased (P＜0.05), and the level of 5-hydroxytryptamine (5-HT) was decreased (P＜0.05). Compared with the model group, the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in each TGCO dose group were decreased gradually (P＜0.05). The expression of choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH) were increased gradually in a dose-dependent manner (P＜0.05). Conclusion: TGCO can effectively improve cognitive dysfunction caused by hypothyroidism, and its mechanism may be related to regulating neurotransmitters, inhibiting inflammatory response and regulating related signaling pathways, which provides a new theoretical basis for clinical treatment.