FAN Zixu, LI Xingnan, JIN Min, WANG Zhanli, GAO Zhixiang
Objective: To explore the mechanism of Mongolian medicine Sendeng-9 Decoction (Brucellosis Ⅱ) in the treatment of brucellosis by network pharmacology. Methods: The BATMAN-TCM database was used to analyze the active components and potential targets of Mongolian medicine Sendeng-9 decoction. Brucellosis-related targets were screened through Genecards, OMIM, and DisGeNET databases. The Venn diagram was constructed to obtain the intersection target of disease and the intersection target of Mongolian medicine Sendeng-9 decoction in the treatment of brucellosis. The network diagram of “drug-component-disease-target” relationship was drawn by Cytoscape software. The protein-protein interaction (PPI) network of ' active ingredient-disease ' common targets was constructed by STRING database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of key targets were performed using the David database. Results: There were 182 active components of Mongolian medicine Sendeng-9 Decoction, which acted on 1306 targets. There were 620 brucellosis targets and 152 drug-disease intersection targets. The main active ingredients of Mongolian medicine Sendeng-9 Decoction in the treatment of brucellosis were lauric acid, myristic acid, linoleic acid, jasmonone, lupinone, geranylacetone, oxysophocarpine, 3-nonen-2-one, etc. The core targets for the treatment of brucellosis were IL-6, IL-1β, INS, TNF, PTGS2, JUN, TP53, IGF1, CCL2, HIF1A. A total of 1064 GO entries were obtained, including 834 BP entries, 107 CC entries, and 123 MF entries. There were 162 KEGG signaling pathways, mainly focusing on HIF-1 signaling pathway, TNF signaling pathway, MAPK signaling pathway, FoxO signaling pathway, IL-17 signaling pathway and so on. Conclusion: Through the method of network pharmacology, this study preliminarily discusses the anti-inflammatory and disease-resistant pathogenic microorganisms of the effective components of Mongolian medicine Senden-9 decoction, which provides a theoretical basis for the treatment of brucellosis, and further study of the mechanism of action and clinical application of this prescription.