Objective: To analyze the core drug pairs and their molecular biological mechanisms for the treatment of hepatitis B cirrhosis based on data mining and network pharmacology. Methods: Retrieve all the literatures related to the application of traditional Chinese medicine in the treatment of hepatitis B cirrhosis from January 2000 to December 2022 in China National Knowledge Infrastructure, Wanfang Database and VIP Science and Technology Journal, and study the medication rules of the prescriptions that meet the standards. The commonly used core drug pairs were subjected to pharmacological analysis, and the active components, main targets, and action pathways were predicted. Finally, molecular docking technology was used to simulate and verify on the computer. Results: A total of 275 prescriptions for the treatment of hepatitis B cirrhosis were screened. The analysis showed that TCM for the treatment of hepatitis B cirrhosis included 13 kinds of drugs such as Huangqi and Danshen. The core combination mode was 28 groups. Quercetin,Luteolin,Kaempferoland other ingredients were found as the main components of core prescription drugs for the treatment of hepatitis B cirrhosis,involving core targets such as serine/threonine protein kinase 1 (AKT1), tumor necrosis factor (TNF), tumor protein p53 (TP53), interleukin-6 (IL-6), vascular endothelial growth factor A (VEGFA), Caspase-3 (CASP3) and phosphatidylinositol-3-kinase/protein kinase (PI3K/Akt), TNF, interleukin-17 (Interleukin-17). Conclusion: The treatment principle of hepatitis B cirrhosis is to strengthen the body and relieve masses. "Huangqi-Danshen" can play a role in regulating cell growth, proliferation, survival and inflammatory reaction by interfering with PI3K-Akt, TNF, IL-17 and other signal pathways, so as to delay the progression of liver cirrhosis in hepatitis B.

Objective: To study and analyze the changes of intestinal flora in 1-5 years after cholecystectomy. Methods: A total of 15 fecal samples of healthy people and 16 fecal samples of people 1 to 5 years after cholecystectomy were collected from Baotou Central Hospital. The collected samples were sequenced to analyze the composition, richness and diversity of intestinal microbial flora in the two groups, and to compare the differences of intestinal microbial flora between the two groups. Results: (1) At the phylum level, the relative abundance of Bacteroidetes, Proteobacteria, Verrucomicrobia and Fusobacteria increased, while the relative abundance of Actinobacteria decreased in the population 1-5 years after cholecystectomy. (2) At the genus level, the relative abundance of Escherichia, Ruminococcus, Blautia, and Gemmatiaceae increased, while the relative abundance of Faecalibacterium, Bifidobacterium, Collins, and Agathobacter decreased in the population 1 to 5 years after cholecystectomy. The Chao1 index and Observed _ species index of the healthy population group were higher than those of the population group 1-5 years after cholecystectomy, and the differences were statistically significant (P＜0.05). The Pielou evenness of the healthy population group was slightly lower than that of the population group 1-5 years after cholecystectomy, and the difference was statistically significant (P＜0.05). Lachnospiraceae, Ruminococcus, Faecalimonas and Burkholderia were the intestinal flora with significant differences between the groups in the population group from 1 to 5 years after cholecystectomy. Conclusion: (1) The composition of intestinal flora in the population 1-5 years after cholecystectomy has changed. (2) The relative abundance of short-chain fatty acid-producing bacteria and probiotics in the population 1-5 years after cholecystectomy decreased, while the relative abundance of pathogenic bacteria increased.

Objective: To explore the genes and mechanisms in colorectal cancer and adjacent tissues at different distances by bioinformatics analysis. Methods: Eukaryotic mRNA was sequenced by Illumina Novaseq 6000 sequencing platform. Results: Differential expression analysis showed that there were 28 000 differentially expressed genes in the upper margin group of colorectal cancer, of which 11 735 were up-regulated and 16 265 were down-regulated. There were 32 438 differentially expressed genes in the lower margin group of colorectal cancer, of which 11 477 were up-regulated and 20 961 were down-regulated. The differentially expressed genes IGF2BP1, ADAM12, CDON, AKAP6, IGSF9B, LONR2 and KCNB1 were found to be down-regulated in colorectal cancer tissues and adjacent tissues at different distances. Functional annotation analysis of differential genes showed that differential genes were mainly involved in cellular processes, and the pathways involved were mainly metabolism, signal transduction and immune system. In terms of pathogenesis, it mainly exists in cancer, nervous system diseases and gastrointestinal system diseases. Differential gene enrichment analysis showed that differential genes were mainly in cardiac conduction, collagen fiber tissue, collagen decomposition process, etc., and the enriched pathways were mainly human papillomavirus infection pathway and P13K-Akt signaling pathway. Conclusion: The expression of differential genes IGF2BP1, ADAM12, CDON, AKAP6, IGSF9 B, LONR2 and KCNB1 is down-regulated in colorectal cancer tissues and adjacent tissues at different distances. Differential genes exist in cells and are mainly involved in metabolic pathways, signal transduction pathways, and immune system pathways. The pathogenesis mainly exists in cancer, nervous system diseases and gastrointestinal system diseases.

Objective: To construct a gene plasmid vector carrying NLRP3 and NLRP12 mutations in Wistar rats according to the NLRP3 (p.V72M, c.214G＞A) and NLRP12 (p.R754H, c.2261G＞A) gene mutation sites of the familial cold autoinflammatory syndrome (FCAS) family. Methods: According to the amino acid homology, the NLRP3 and NLRP12 gene mutation plasmids of rats were designed. The target genes were amplified by polymerase chain reaction (PCR) and recovered. The double enzyme digestion and connection were performed on the pCMV-mCherry-MCS-Neo vector. The connected products were transformed into competent cells. The positive transformants were identified by colony PCR, plasmid extraction electrophoresis and sequencing. Results: Agarose gel electrophoresis showed that the NLRP3 and NLRP12 mutant genes were successfully amplified. The gene mutation plasmid was confirmed by enzyme digestion electrophoresis and DNA sequencing. The gene sequence was completely correct and the recombinant plasmid vector was successfully constructed. Conclusion: The plasmid vectors of NLRP3 and NLRP12 gene mutations is successfully constructed, which provides a biological basis for further exploring the pathogenesis of FCAS caused by NLRP3 and NLRP12 gene mutations and the functional study of NLRP3 and NLRP12 genes.

Objective: To investigate the relationship between the activity and gene polymorphism of coagulation factor Ⅻ and the risk of recurrent spontaneous abortion (RSA) in Baotou Han population. Methods: RSA patients and healthy pregnant women were selected as the research objects. The activity of coagulation factor Ⅻ was measured. The genotype composition of FⅫ gene single nucleotide polymorphism (SNP) rs1801020 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results of the two groups were analyzed to determine the correlation between FⅫ gene SNP rs1801020 and RSA under different genetic models. Results: There were significant differences in the genotype and allele frequency distribution of SNP rs1801020 in FⅫ gene between the two groups (P＜0.05); the frequency of T allele in RSA group was lower than that in control group (χ²=8.756, OR=2.043, 95%CI: 1.267-3.295,P<0.05); the frequency of CT genotype in RSA group was higher than that in control group, while the frequency of TT genotype was lower than that in control group, and the difference was statistically significant (χ²=9.905, OR=2.611, 95%CI: 1.447-4.713,P＜0.05); FⅫ activity in RSA patients was different among rs1801020 genotypes, and the order of activity was CC, CT and TT. FⅫ SNP rs1801020 was associated with the risk of RSA under codominant, dominant and overdominant models (OR=2.611, 2.636, 2.470, P＜0.05); compared with TT genotype, CT and CT+CC genotypes could increase the risk of RSA; compared with CC+TT genotype, CT genotype could increase the risk of RSA. Conclusion: The difference in the distribution of FⅫ rs1801020 between the two groups may be related to the risk of RSA in Baotou Han women. Compared with TT genotype, mutant heterozygous CT genotype may increase the risk of RSA, the T allele of rs1801020 locus of FⅫ gene can cause the decrease of FⅫ activity.

Objective: To systematically evaluate the efficacy and adverse reactions of epirubicin (EPI) and mitomycin (MMC) in patients with non-muscle invasive bladder cancer (NMIBC) after perfusion chemotherapy. Methods: CNKI, Wanfang, VIP, SinoMed, PubMed, Embase, The Cochrance Library and Web of science were searched for Chinese and English randomized controlled trials of epirubicin and mitomycin in the treatment of NMIBC from the establishment of the database to January 2023. RevMan 5.3 software was used to perform a meta-analysis of postoperative tumor recurrence rate, total incidence of adverse reactions, incidence of bladder irritation, liver and kidney function damage, and hematuria in the EPI group and the MMC group. Results: There were 16 studies that met the inclusion and exclusion criteria, with a total of 1244 patients, while 626 patients in the EPI group and 618 patients in the MMC group. The results of meta-analysis showed that the postoperative tumor recurrence rate [OR=0.55,95%CI(0.41, 0.73), P＜0.01], the incidence of total adverse reactions [OR=0.56,95%CI(0.38, 0.82), P=0.003], the incidence of bladder irritation [OR=0.54, 95%CI(0.37, 0.77), P=0.0007], the incidence of hematuria [OR=0.53,95%CI(0.33, 0.85), P=0.009], and the incidence of liver and kidney dysfunction [OR=0.26, 95%CI(0.10, 0.69), P=0.007] in the EPI group were significantly lower than those in the MMC group (P＜0.05). Conclusion: EPI is superior to MMC in terms of therapeutic effect and total incidence of adverse reactions. EPI bladder perfusion is recommended to prevent tumor recurrence and improve the prognosis of patients after bladder cancer surgery.

Objective: To detect differentially expressed miRNAs in exosomes derived from intestinal epithelial cells of essential hypertensive rats, and to enrich and analyze their target genes. Methods: Three Spontaneously hypertensive rats (SHR) and three Wistar-Kyoto rats (WKY) were selected as the research objects. The expression of miRNA in exosomes derived from intestinal epithelial cells of rats was detected by high-throughput sequencing. The differentially expressed miRNAs in the two groups of rats were screened and their target genes were predicted. The target genes were analyzed by Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) using biological function software. Results: Compared with the WKY group, there were 37 differentially expressed miRNAs in the SHR group (All P＜0.05), 32 miRNAs were up-regulated and 5 miRNAs were down-regulated. Among them, rno-miR-208b-3p, rno-miR-134-5p, rno-miR-93-3p, rno-miR-378b, rno-miR-96-5p, rno-miR-210-3p, and rno-miR-208a-3p were noteworthy. A total of 10 662 miRNA downstream target genes were predicted, and a total of 34 127 target sites were predicted. GO enrichment was mainly concentrated in membrane-bound organelles, protein binding, and positively regulated biological processes. KEGG enrichment was mainly concentrated in MAPK, mTOR, Ras and TNF signaling pathways. Conclusion: The expression of miRNA in exosomes derived from intestinal epithelial cells of essential hypertensive rats was significantly different. Its target genes may be involved in MAPK, mTOR, Ras and TNF signaling pathways through cell growth and differentiation, angiogenesis and metabolic function, thus affecting the occurrence and development of hypertensive diseases.

Objective: To analyze the expression and correlation of human papillomavirus type 16 E6 protein (HPV16-E6), inhibitor of intracellular differentiation-1 (ID-1) and prolylcis-trans isomerase1 (Pin1) in cervical cancer. Methods: A total of 65 patients with cervical cancer admitted to the Affiliated Hospital of Putian University from March 2017 to March 2022 were selected. All patients underwent surgical resection of cancer tissues in our hospital, and cervical cancer tissues and adjacent tissues more than 2 cm from the edge of cancer tissues were retained. The positive expression rates of HPV16-E6, ID-1 and Pin1 in cancer tissues and adjacent tissues were detected by immunohistochemistry. The relationship between HPV16-E6, ID-1, Pin1 and clinical features was analyzed. Results: Compared with adjacent tissues, the positive rates of HPV16-E6, ID-1 and Pin1 in cancer tissues were higher (P＜0.05). HPV16-E6, ID-1 and Pin1 were positively correlated with depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter (P＜0.05).. After controlling for age, pathological type and other factors, HPV16-E6, ID-1 and Pin1 were correlated with depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter (P＜0.05). Conclusion: HPV16-E6, ID-1 and Pin1 are highly expressed in patients with cervical cancer, and HPV16-E6, ID-1 and Pin1 are related to the depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter in patients with cervical cancer.

Objective: To investigate the differences in the occupation, age, gender and serum agglutination test (SAT) antibody titers between asymptomatic Brucellosis cases and acute Brucellosis cases, with the purpose of providing basic information to the prevention and treatment of Brucellosis. Methods: Using a completely random sampling method, 164 acute brucellosis patients and 160 latent brucellosis infections diagnosed according to the "Diagnostic Criteria for Brucellosis" in the Ximeng region from January to December 2019 were selected as the research subjects.Compare the patient's age and SAT titers after logarithmic transformation using two independent sample t-tests; Compare occupation and gender using χ² test.Results: Comparison of occupation between the two groups of patients, the differences were statistically significant (χ²=15.837, P＜0.05). Comparison of age and SAT titer in the two groups, the differences were statistically significant (P＜0.05). Patients with asymptomatic Brucellosis were younger than acute Brucellosis cases, and the SAT titer of symptomatic Brucellosis cases was higher than acute Brucellosis cases. There was no significant difference in gender between the two groups of patients (χ²=0.018, P＞0.05). Conclusion: The infection status of brucellosis may be related to occupation, age, and SAT antibody titers.

Objective: To analyze the related indexes of vaginal microecology that affect the infection and outcome of high-risk human papillomavirus (HR-HPV), and to explore the correlation between HR-HPV and vaginal microecology, so as to provide the corresponding basis for the diagnosis and treatment of HR-HPV infection. Methods: Retrospective analysis of patients who underwent HR-HPV and vaginal secretion tests at the First Affiliated Hospital of Baotou Medical College from September 2021 to March 2023. According to the inclusion criteria, 1 052 cases of HR-HPV infection were selected, while 526 cases in the positive group and 526 cases in the negative group, and 131 cases in the returned portion: 76 cases in the returned group and 55 cases in the persistently infected group. The differences in the vaginal microecology of the above patients were compared between the groups. Results: (1) Age was correlated with HR-HPV infection and regression (P＜0.05). (2)The proportion of abnormal vaginal cleanliness, bacterial vaginitis (BV), pH＞4.5, H₂O₂(+),leukocyte esterase (LE) (+),and sialidase (SNA) (+)in the HR-HPV positive group was higher than that in HR-HPV negative group (P＜0.05); abnormal cleanliness, and SNA(+) were the independent risk factors for HR-HPV infection (P＜0.05). (3)The proportion of abnormal flora density, abnormal diversity, abnormal cleanliness, BV, pH＞4.5, H₂O₂(+), LE(+), SNA(+) in persistently infected group was higher than that of the regression group (P＜0.05); abnormal density, pH＞4.5, H₂O₂(+), LE(+) were the independent risk factors for the persistently infected of HR-HPV (P＜0.05); there was no change in the comparison of vaginal cleanliness before and after the regression of HR-HPV (P＞0.05). Conclusion: Vaginal microecological imbalance is associated with HR-HPV infection and persistence. Bacterial vaginitis, elevated pH value and abnormal H₂O₂ are risk factors for HR-HPV infection and persistence.

Objective: To investigate the correlation between obstructive sleep apnea (OSA) and lung function and cognitive impairment, and to investigate the possible mechanisms of lung function decline and cognitive impairment caused by OSA. Methods: Forty-six patients with obstructive sleep apnea hypopnea syndrome (OSAHS) diagnosed by polysomnography (PSG) from January 2021 to June 2022 were collected. According to the apnea hypopnea index (AHI), they were divided into mild to moderate group (n=20) and severe group (n=26). Healthy subjects (n=23) were selected as the control group. The general data, PSG, lung function, blood gas analysis, and Montreal Cognitive Assessment Scale (MoCA) scores of the subjects were compared and statistically analyzed. Results: The vital capacity (VC), forced expiratory volume in 1 second percent predicted (FEV1%pred), forced expiratory volume in one second/forced vital capacity (FEV1/FVC%), expiratory reserve volume (ERV), functional residual capacity (FRC), maximum mid-expiratory flow rate (MMEF%) and 50% forced expiratory flow rate (FEF50%) in the severe group were lower than those in the mild-to-moderate group and the healthy group (P＜0.05). The partial pressure of oxygen (PaO₂) in the severe group was lower than that in the mild to moderate group and the healthy group (P＜0.05). There was significant difference in MoCA score among the three groups (P＜0.05). Correlation analysis showed that PaO₂ was negatively correlated with AHI and the longest apnea time, and positively correlated with LSaO₂. Conclusion: The lung function and cognitive ability of OSA patients are impaired, and the lung function and cognitive ability of patients with severe OSA are more obvious. Night hypoxia and low daytime PaO₂ play an important role in the mechanism of lung function decline and cognitive impairment in OSA patients.

Objective: To analyze the effect of cone-beam computed tomography (CBCT) guided radiotherapy for malignant tumors. Methods: A total of 68 patients with malignant tumors who received radiotherapy from October 2021 to October 2022 were selected as the research objects. They were randomly divided into observation group and control group. Among them, 34 patients in the observation group received CBCT-guided radiotherapy, with a frequency of 1 time/d. 34 patients in the control group also received CBCT-guided radiotherapy, with a frequency of 1 time/3 d. The clinical effects of CBCT-guided methods were compared and analyzed. Results: The absolute values of x, y, z axis setup errors and setup expansion boundary in the observation group were lower than those in the control group (P＜0.05). The maximum irradiation dose and average irradiation dose of malignant tumor patients in the gross tumor area and planned target area in the observation group were higher than those in the control group (P＜0.05). The objective effective rate of the observation group was higher than that of the control group (P＜0.05). The quality of life scores in all fields of the observation group were higher than those of the control group (P＜0.05). Conclusion: For patients with malignant tumors, CBCT scanning and guidance before treatment can reduce the positioning error during radiotherapy. With the increase of CBCT scanning frequency, the adaptability and accuracy of radiotherapy can be better improved, the treatment effect can be ensured, and the discomfort reaction can be prevented.

Objective: To investigate the protective mechanism of TAK-242 on hippocampal inflammatory injury in rats with post-stroke depression. Methods: A total of 54 male 3-month-old SD rats were divided into sham operation group (n=18), post-stroke depression (PSD) model group (n=18) and TAK group (n=18). The middle cerebral artery occlusion (MCAO) model was established by suture method, and then chronic unpredictable mild stress (CUMS) stimulation was performed for 3 weeks to establish the PSD model. After the PSD model was successfully established, the TAK group was intraperitoneally injected with diluted TAK-242 0.3 mg/kg, which was performed on the 5th, 10th, 15th and 20th day of CUMS, respectively. The level of IL-1β in peripheral blood was detected by enzyme-linked immunosorbent assay. Results: Compared with the sham operation group, the body weight, sucrose preference index and movement (LA) of the PSD group and the TAK group were lower than those of the sham operation group, and the level of IL-1β was significantly increased (P＜0.05). Compared with PSD group, the body weight, sucrose preference index and LA of rats in TAK group were increased, and the expression level of IL-1β was decreased (P＜0.05). Conclusion: TAK-242 can reduce the expression of IL-1β in peripheral blood by inhibiting TLR4 signaling pathway, thus exerting a neuroprotective effect on hippocampal inflammatory injury in rats with post-stroke depression, and providing new ideas for clinical prevention and treatment of post-stroke depression.

Objective: To observe the expression levels of regulatory T cells (Treg), helper T cells 17 (Th17) and cytokine interleukin 17 (IL-17) in peripheral blood of patients with type 1 diabetes mellitus (T1DM) and latent autoimmune diabetes in adults (LADA), with the purpose of exploring the role of Th17/Treg in T1DM by analyzing its correlation with islet-reactive B cells function in T1DM. Methods: A total of 78 cases of T1DM patients, LADA patients and normal healthy people who were admitted in the Endocrinology Department, the First Affiliated Hospital of Bengbu Medical College from December 2021 to June 2022 were selected and divided into the T1DM group, LADA group and normal control (NC) group. Serum IL-17 was detected by ELISA method. Proportions of Th17 and Treg cells were detected by flow cytometry, and the differences of Th17 and IL-17 expression among the three groups were compared. Results: The proportion of Treg cells in the T1DM group and LADA group was significantly lower than that in the NC group, and it was significantly lower in the T1DM group than that in the LADA group. The expression levels of Th17 and IL-17 in the T1DM group and LADA group were significantly higher than those in the NC group, but there was no significant difference between the T1DM group and LADA group. The results of Pearson correlation analysis showed that serum IL-17 and Th17 were negatively correlated with FCP and 2h-CP (IL-17: r/P=-0.273, 0.016, -0.352, 0.002), Treg was positively correlated with FCP and 2h-CP (r/P=0.494/<0.001, 0.575/<0.001). Multivariate logistic regression analysis showed that IL-17 was a risk factor to T1DM and LADA by taking T1DM, LADA, NC as dependent variables(1=T1DM,2=LADA,3=NC), Th17 was a risk factor to T1DM, and Treg was a protective factor to T1DM and LADA. Conclusion: T1DM and LADA patients have unbalanced Th 17 / Treg ratio in vivo, manifested by increased expression level of Th 17 and IL-17 and decreased expression level of Treg, it may be associated with functional impairment of islet-reactive B cells, which could lead to the occurrence and development of T1DM.

Objective: To investigate the active ingredients and mechanism of Sophora alopecuroides L. in the treatment of alcoholic liver disease (ALD) based on network pharmacology and molecular docking techniques. Methods: The active ingredients in Sophora alopecuroides L. were screened by Herb platform, and the corresponding targets of active ingredients were searched in SIB database. The targets of alcoholic liver disease were obtained from NCBI and GeneCards databases. The mapping tool Venny 2.1 was used for intersection comparison to obtain common targets. The protein interaction analysis and visual presentation were performed on the String platform. The DAVID database was used for GO function and KEGG pathway enrichment analysis. Finally, AutoDock and PyMOL software were used for molecular docking. Results: Eight active ingredients and 97 effective targets were identified by network pharmacology. The mechanism of action might be related to cancer pathway, lipid and atherosclerosis, T cell receptor signaling pathway and VEGF signaling pathway, and the main targets involved were HSP90AA1, AKT1, FYN and AKT2. Molecular docking results showed that matrine, sophocarpine and sophoramine had good binding force with HSP90AA1, FYN and AKT1. Conclusion: Matrine, sophocarpine and sophoramine in Sophora alopecuroides L. can ameliorate ALD by inhibiting inflammatory response or apoptosis through multi-target and multi-pathway interactions.

急性胰腺炎(acute pancreatitis, AP)是一种严重的消化系统疾病,其发生和发展与多种因素相关。近年来,肠道菌群失衡被认为是影响AP进程的重要因素。研究表明,AP患者的肠道菌群多样性减少、有益菌与致病菌比例失衡、特定菌群丰度的变化等,均通过影响肠道屏障功能和激发炎症反应影响AP进程。肠黏膜屏障在维持机体免疫防御系统完整性和正常生理功能方面起着至关重要的作用。肠道菌群失衡导致肠黏膜屏障功能受损,使肠道通透性增加,细菌发生易位,引发全身炎症反应综合征甚至多器官功能衰竭,加重胰腺炎病情,影响患者预后。此外,肠道菌群失衡还导致代谢产物的变化,如短链脂肪酸(short-chain fatty acids, SCFAs)和胆汁酸等,这些变化对胰腺功能和AP的发展有着重要影响。因此,维持肠道菌群平衡可能对AP的治疗和预后具有重要意义。笔者寻找对AP至关重要的特定菌株或参与AP的其他代谢产物,通过调节AP患者肠道菌群作为治疗AP的一种有效方法,深入研究肠道菌群与AP之间的相互作用,不仅有助于揭示AP的病理机制,还可能为AP的预防和治疗提供新的策略和方法。

Objective: To investigate the effect and mechanism of puerarin (Pue) in the treatment of postmenopausal osteoporosis (PMOP) in rats. Methods: Female SD rats were divided into sham operation group (SHAM group), model group (PMOP group), low-dose puerarin group (Pue-L group) and high-dose puerarin group (Pue-H group). In the SHAM group, partial fat around the ovary was removed, and bilateral ovaries were removed in the other groups. Bone mineral density (BMD) and hematoxylin-eosin (HE) staining were used to observe the femoral tissue at 4 weeks after operation to determine whether the model was successful. After successful modeling, rats in Pue-L group and Pue-H group were subcutaneously injected with different concentrations of puerarin injection, and rats in SHAM group and PMOP group were injected with the same amount of normal saline. BMD of rats was detected after 8 weeks of administration. The bone structure was observed by micro-CT scanning and the related parameters were calculated. HE staining was used to observe the pathological changes of rat femur. The protein expression level of JAK2/STAT3 signaling pathway was detected by Western Blot (WB). Results: (1) Pue significantly increased BMD in PMOP rats. (2) Micro-CT analysis showed that Pue could improve the bone microstructure of PMOP rats. (3) HE staining showed that after Pue treatment, the bone loss of PMOP rats decreased, the number and density of trabecular bone increased, and the pathological changes were restored. (4) WB results showed that compared with SHAM group, the expression levels of p-JAK2 and p-STAT3 protein in PMOP group were increased (P＜0.001). Compared with PMOP group, the expression levels of p-JAK2 and p-STAT3 protein in Pue-L group and Pue-H group were decreased (P＜0.001). Conclusion: Puerarin can effectively treat osteoporosis in PMOP rats, which may play an anti-osteoporosis role by inhibiting the activation of JAK2/STAT3 pathway.

Objective: To analyze the proportion of SPP1⁺ macrophages in peripheral blood of patients with connective tissue disease complicated with interstitial lung disease (CTD-ILD) and the expression level of its expression product chemokine 18 (CCL18) in serum of patients with CTD-ILD and its clinical significance. Methods: Twenty patients with connective tissue disease (CTD) and 20 healthy people were selected as the control group, and 35 patients with newly diagnosed CTD-ILD were selected as the experimental group. The differences in the expression levels of SPP1⁺ macrophages and CCL18 in the experimental group and the control group were compared, and the correlation with type I collagen (COL1A1), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) was analyzed. Results: Compared with the control group, the proportion of SPP1⁺ macrophages in peripheral blood mononuclear cells of CTD-ILD patients was significantly increased (P＜0.05), and the content of CCL18 in serum was also significantly increased (P＜0.05). At the same time, SPP1⁺ macrophages and CCL18 were positively correlated with ESR, CRP, COL1A1 and lung imaging score (HRCT score) (P＜0.05), and negatively correlated with the percentage of forced vital capacity (FVC%) and the percentage of carbon monoxide diffusion (DLCO%) (P＜0.05). The area under the curve (AUC) of SPP1⁺ macrophages and CCL18 were 0.732 and 0.939, respectively. The AUC of the combined detection of SPP1⁺ macrophages and CCL18 was 0.939, the sensitivity was 93.5%, the specificity was 83.3%, and the Youden index was 0.768. Conclusion: SPP1⁺ macrophages and CCL18 are poor prognostic factors in patients with CTD-ILD. The combined detection of SPP1⁺ macrophages and CCL18 is of great significance for the prognosis of the disease.

目的: 通过Meta整合并分析了关于经皮冠状动脉介入治疗(percutaneous coronary intervention,PCI)术后患者心脏康复需求的质性研究的中英文文献,旨在满足患者的心脏康复需求,为患者提供有针对性的康复治疗计划。方法: 计算机检索中文数据库:中国知网(CNKI)、万方(WanFang)、维普(VIP)、中国生物医学文献服务系统(Sinomed);计算机检索外文数据库:Medline、Pubmed、荷兰医学文摘数据库(Embase)、护理学全文数据库(CINAHL)、Proquest、Elsevier、Web of Science,收集从建库到2022年9月涉及PCI患者心脏康复需求的质性研究。结果: 共纳入8篇文献,58个研究结果归纳为10个新的类别,并合并为3个结果,即:提供健康教育及二级预防指导,拓宽患者获取信息渠道,建立医院-家庭-社会的支持环境。结论: 医护工作者需重视PCI术后患者对心脏康复的需求,给予患者专业知识和情感支持,搭建医护沟通平台,提供延续性护理,进行个体化心脏康复指导,提高其对自身疾病的认识,促进健康行为,最终改善PCI术后患者生活质量。

Objective: To observe the effects of ribokinase (Rbks) gene knockout on phenotype and glucose and lipid metabolism in C57BL/6J mice. Methods: 12 male 8-week-old wide type (WT) and Rbks gene knockout (KO) C57BL/6J mice were fed for 10 weeks. The changes of appearance, body weight, food intake, water intake and fasting blood glucose were observed regularly. At the end of the observation period, the contents of triglyceride and cholesterol were measured. Results: After being fed normally for 10 weeks, compared with WT mice, KO mice had no significant difference in appearance and body weight(P＞0.05), but food intake and water intake decreased, fasting blood glucose level increased significantly(P＜0.05), serum triglyceride content increased and total cholesterol content decreased (all P＜0.05). Conclusion: Although Rbks gene knockout has no effect on the shape and weight of mice, it will affect the balance of glucose and lipid metabolism in mice.