Objective: To explore the role of lipoyltransferase (LIPT1) in the development and immune infiltration of breast cancer by bioinformatics. Methods: TCGA, GEPIA, HPA, UALCAN, Kaplan-Meier, TIMER 2.0 and other databases were used to explore the differential expression, function and related mechanism of LIPT1 in breast cancer, and to analyze the effect of LIPT1 on the immune infiltration of invasive breast cancer. Results: The expression of LIPT1 was associated with invasive breast cancer, and PPI network analysis showed that LIPT1 was associated with ACSM1, DBT, DLAT, DLT, DLST, GCSH, LIAS, LIPT2, PDHB, and PDHX; the expression of LIPT1 was associated with immune cell infiltration in invasive breast cancer. Conclusion: Bioinformatics predicts that LIPT1 expression is down-regulated in invasive breast cancer and is associated with tumor immune cell infiltration.

Objective: To study the association between single nucleotide polymorphism (SNP) in YAP gene and the risk of colorectal cancer. Methods: YAP rs11225163 was genotyped by Taqman technique in 390 colorectal cancer cases and 413 controls. The odds ratio (OR) and its 95% confidence interval(CI) were calculated by unconditional logistic regression to evaluate the relationship between rs11225163 and the risk of colorectal cancer, rectal cancer and colon cancer under five genetic models: co-dominant, dominant, recessive, overdominant and Log-additive. Results: No associations were found between SNP rs11225163 and the risk of colorectal cancer, rectal cancer and colon cancer under 5 genetic models. Conclusion: YAP gene SNP rs11225163 may not play a major role in the development of colorectal cancer, rectal cancer and colon cancer.

Objective: To investigate the relationship between 25-hydroxyvitamin D and motor symptoms of Parkinson's disease (PD). Methods: A total of 105 PD patients admitted in our hospital from January 2019 to December 2021 were selected as the PD group. The total scores of tremor, postural instability/gait difficulty (PIGD) were calculated according to MDS-UPDRS Ⅱ~Ⅲ item. The PD patients were divided into the tremor-dominant (TD) type (n=33), postural instability/gait difficulty (PIGD) type (n=48), and middle type (n=24). During the same period, 50 elderly health checkups were used as a control group, 25-hydroxyvitamin D, ferritin, albumin, serum uric acid of the two groups of patients were detected. Results: The incidence of vitamin D deficiency in the PD group was 82.90%,which was higher than that in healthy control group 68.00% (34/50), and the difference was statistically significant (P＜0.05). The average level of 25-hydroxyvitamin D in the PD group was 15.16±5.13 ng/L, lower than that of 17.53±4.88 ng/L in control group, and the difference was statistically significant (P＜0.05). The level of 25-hydroxyvitamin D in the PIGD type (13.22±4.82 ng/L) was lower than that in the TD type (17.11±4.85 ng/L) and middle type (16.37±4.90 ng/L) PD patients, and the differences were statistically significant (P＜0.017). There was a strong negative correlation between 25-hydroxyvitamin D level and MDS-UPDRS Ⅲ score (rs=-0.645). Unitary linear regression analysis of 25-hydroxyvitamin D level and MDS-UPDRS Ⅲ score on one-way linear regression analysis (B=-2.556, P=0.000). Conclusion: The incidence of vitamin D deficiency is high in PD patients, and the vitamin D level is lower in the PIGD type PD patients. Vitamin D level is correlated with motor typing and severity of motor symptoms in PD patients.

Objective: To investigate the mechanism of H. pylori NapA protein inducing the secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in macrophages. Methods: Macrophages were stimulated by NapA, then chemokines MCP-1 and IL-8 in supernatant were detected through ELISA. Macrophages were pre-incubated with C29, ST2825, SB203580, SP600125 and PDTC for 1 h to inhibit the activity of TLR2, MyD88, p38, c-Jun and NF-κB, respectively. Then NapA protein was added and incubated for 4 h. The supernatant was collected and the expression levels of MCP-1 and IL-8 were examined. Results: The expression levels of MCP-1 and IL-8 in macrophages stimulated by H. pylori NapA protein were significantly higher than those in untreated group. When the activity of TLR2 was inhibited by C29, the expression of MCP-1 and IL-8 induced by NapA protein decreased. Inhibition of MyD88 NF-κB and p38 decreased the ability of macrophages to secrete MCP-1 and IL-8 induced by NapA protein. However, inhibition of c-Jun did not affect the secretion of MCP-1 and IL-8 by macrophages induced by NapA protein. Conclusion: H. pylori NapA protein induces macrophages to secrete chemokines MCP-1 and IL-8 via TLR2/MyD88/NF-κB pathway.

Objective: To explore the effect of GPIa C807T and G873A gene polymorphisms on platelet aggregation inhibition rate, aiming to provide experimental data for clinical individualized antiplatelet aggregation therapy. Methods: A total of 80 inpatients receiving antiplatelet treatment with aspirin were collected and divided into two groups according to the inhibition rate of platelet aggregation detected by thromboelastography.10 patients were divided into the aspirin resistance (AR) group and 70 patients into the aspirin sensitive (AS) group. The genotypes of GPIa C807T, G873A and their allele distribution in the two groups were analyzed, and the relationship between clinical data and experimental indicators of the two groups were also analyzed. Results: The 807T and 873G alleles in the AR group were higher than those in the AS group (χ²＝4.039, 4.354, P=0.044, 0.037). The prevalence of hypertension and stroke history in the AR group was higher than that in the AS group (χ²＝4.612, 4.737, P=0.032, 0.030). Plasma D-dimer (D-D) and serum triglyceride (TG) in the AR group were higher than those in the AS group (t=2.499, 2.276, P=0.016, 0.033), while platelet aggregation inhibition rate (IPA) and serum glucose (GLU) in the AR group were lower than those in the AS group (t=15.352, 3.076, P＜0.001, 0.005). Conclusion: GPIa C807T and G873A gene polymorphism were correlated with aspirin resistance. Joint detection of the two gene loci polymorphisms and platelet aggregation inhibition rate was recommended to jointly guide the clinical individualized antiplatelet aggregation therapy.

Objective: To investigate the effect of miR-140-3p on hypoxia/reoxygenation-induced myocardial cell reperfusion injury by targeting PI3K/Akt pathway. Methods: H9C2 cardiomyocytes derived from rats were subjected to hypoxia/reoxygenation to induce cell injury. MiR-140-3p mimics and their control (NC) were transfected into H9C2 cardiomyocytes, and H9C2 cells overexpressing miR-140-3p were treated with 10 μmol/L LY294002. The cardiomyocytes were divided into 6 groups, named control group (CON group), H/R group (Model group), Model+NC group, Model+miR-140-3p mimics group, Model+miR-140-3p mimics+LY294002 group, Model+LY294002 group. The relative expression of miR-140-3 p in each group was detected by fluorescence quantitative PCR (RT-PCR). The viability of cardiomyocytes in each group was detected by CCK-8 method. The apoptosis rate of cardiomyocytes in each group was detected by flow cytometry. The expression levels of phosphorylated phosphatidylinositol 3 kinase (p-PI3K), phosphorylated protein kinase B (p-Akt), Bax, Bcl-2, and cleared caspases-3 in each group were detected by Western blot. Results: Compared with the CON group, the expression level and cell viability of miR-140-3p, as well as the expression of Bcl-2, p-PI3K, and p-Akt proteins, were significantly reduced in the Model group (P＜0.05), while the expression and apoptosis rate of Bax and Cleared caspases-3 proteins were significantly increased (P＜0.05). Compared with the Model group, overexpression of miR-140-3p upregulated the expression level and cell viability of miR-140-3p, as well as Bcl-2, p-PI3K, and p-Akt proteins, while downregulated the expression and apoptosis rate of Bax and Cleared caspases-3 proteins, LY294002 could inhibit the expression of miR-140-3p, downregulate cell viability and Bcl-2, p-PI3K, p-Akt proteins, and upregulate the expression of Bax, Cleared caspases-3 proteins and apoptosis rate. Conclusion: MiR-140-3p can alleviate autophagy and apoptosis in hypoxia/reoxygenation cardiomyocytes by regulating the PI3K/Akt pathway, thereby alleviating myocardial injury.

Objective: To investigate the regulatory mechanism of miR-152-3p and DNA Methyltransferases(DNMT1) in colon cancer cells. Methods: qRT-PCR was used to detect the expression of miR-152-3p and DNMT1. Cell proliferation assay, colony formation assay, scratch healing assay and Transwell assay were used to detect the proliferation, migration and invasion ability of cancer cells in each treatment group. Dual luciferase assay was used to detect the targeting relationship between miR-152-3p and DNMT1. Western blot assay was used to detect the protein expression of DNMT1. Flow cytometry assay was used to detect apoptosis rate of cells. Results: MiR-152-3p was significantly downregulated while DNMT1 was highly expressed in colon cancer cells. Overexpression of miR-152-3p inhibited the proliferation, migration and invasion of colon cancer cells. MiR-152-3p could inhibit the expression of DNMT1, thereby inhibiting the proliferation, migration and invasion of colon cancer cells. Overexpression of miR-152-3p could target DNMT1 and promote cell apoptosis. Conclusion: MiR-152-3p could downregulate the expression of DNMT1, thereby inhibiting the development of colon cancer cells.

Objective: To study the effect of sepsis on myocardial injury and the intervention effect of vitamin C in rats. Methods: Ninety SD male rats were randomly divided into three groups.One group was treated with vitamin C (group A), the other group was treated with vitamin C as sepsis group (group B) and the last group was control group (group C).The levels of procalcitonin (PCT), lipopolysaccharide (LPS) and superoxide dismutase (SOD) were detected at 24 h and 48 h after successful modeling.The changes of left ventricular ejection fraction, left ventricular end-systolic and end-diastolic volume, myocardial cell action potential and ion channel voltage and density were detected 24 hours after modeling. Results: The levels of PCT and LPS in group A and group B were higher than those in group C, while the levels of PCT and LPS in group B were higher than those in group A at 48 h after modeling.Ultrasound showed that the left ventricular ejection fraction, systolic and end-diastolic volume of rats in group A and group B decreased, but group B was more significant.Compared with group C, the action potential of myocardial cells in group A and group B was significantly prolonged, while that in group B was more obvious.Compared with group C, the density of I_Ca-Land I_to in group A and group B was significantly lower than that in group C, and that in group B was more significant (all P＜0.05).At 48 hours after modeling, SOD detection showed that group A was higher than group C, and group B was lower than group C (all P＜0.05). Conclusion: PCT and LPS increased in septic rats, and vitamin C intervention can reduce their levels; SOD decreased in septic rats, and vitamin C intervention could reduce the level of SOD.The cardiac function of septic rats decreased, and the action potential of myocardial cells was significantly prolonged.At the same time, the density ofI_Ca-Land I_to in septic rats decreased, which could be improved after vitamin C intervention.

Objective: To analyze the effects of Helicobacter pylori on blood pressure, carotid atherosclerosis, long non-coding RNA (LncRNA) MALAT1, nitric oxide (NO), corticosterone and new cardiovascular and cerebrovascular diseases. Methods: The patients with both hypertension and Helicobacter pylori infection were divided into experimental group and control group. 296 cases each.The control group was treated with antihypertensive and lipid-lowering treatment, and the experimental group was treated with anti-Helicobacter pylori drugs on this basis. Then the changes of blood pressure, blood lipid level, carotid intima thickness and heart rate were compared between the two groups. After 2 years of follow-up, the proportion of new cardiocerebral artery occlusion was observed. Thirty-two 6-week-old male rats were divided into spontaneously hypertensive rats (SHR) group A and group B, Wistar-Kyoto (WKY) rats group C and group D, with 8 rats in each group. Rats in group A and group C were given Helicobacter pylori gavage, and rats in group B and group D were given 0.9% NaCl gavage. After 14 weeks, blood and gastric tissue were taken for detection. Results: (1) Helicobacter pylori affected blood pressure, so that the blood pressure of SHR and WKY rats were significantly increased; the degree of hypertension in SHR was more obvious (P＜0.05). (2)The increase of Helicobacter pylori value affected the levels of LncRNA MALAT1, NO and corticosterone (P＜0.05). (3) The proportion of new heart and cerebral artery insufficiency or even occlusion after the decrease of Helicobacter pylori value was less than that of the control group (P＜0.05). Conclusion: (1)The content of Helicobacter pylori is positively correlated with the increase of blood pressure, LncRNA MALAT1 and corticosterone, and negatively correlated with NO. (2) After the treatment of Helicobacter pylori, it can reduce the degree of blood pressure level and the proportion of coronary cerebral artery occlusion.

Objective: To observe the clinical effect and safety of total glucoside of paeony combined with bicyclol in the treatment of mild autoimmnue hepatitis. Methods: A total of 56 patients with mild AIH admitted from April 2020 to April 2023 were randomly divided into treatment group and control group, with 28 patients in each group. The treatment group was given total glucosides of paeony (0.6 g/time, 3 times/d) combined with bicyclol (50 mg/time, 3 times/d) oral treatment, while the control group was only given bicyclol (50 mg/time, 3 times/d) oral treatment. The liver inflammation indexes, immunological related indexes and liver fibrosis indexes of the two groups were measured at baseline, 12 weeks and 24 weeks of treatment, and the drug-related adverse reactions were recorded. Results: After 24 weeks, the total effective rates of the treatment group and the control group were 89.29% and 60.71%, respectively, and the difference was statistically significant (P=0.037). There were significant differences in ALT, AST, CD4⁺T(%), CD8⁺T(%), total globulin and IgG levels between the two groups (P＜0.001), but there was no significant difference in ALP and liver stiffness measurement (LSM) between the two groups. Compared with the control group, the levels of ALT, AST, CD8⁺T(%), total globulin and IgG in the treatment group decreased, and the level of CD4⁺T(%) increased after 24 weeks. Compared with the baseline, the LSM values of the two groups decreased after 24 weeks. There was no significant difference in the incidence of adverse reactions between the two groups (P=1.0). Conclusion: Total glucosides of paeony combined with bicyclol can better improve the level of liver inflammation in patients with mild AIH, regulate the immune status of the body, control the progression of liver fibrosis and do not increase the incidence of adverse drug reactions.

Objective: To investigate the effect of glycated hemoglobin control level on gonadal hormone, body mass index, blood lipid and visceral fat area in middle-aged and elderly male patients with type 2 diabetes mellitus, and to analyze the related factors affecting testosterone. Methods: A total of 90 middle-aged and elderly male patients with type 2 diabetes mellitus were selected and divided into good blood glucose control group (30 patients, glycosylated hemoglobin＜7%), poor blood glucose control group (30 patients, glycosylated hemoglobin 7%～9%) and bad blood glucose control group (30 patients, glycosylated hemoglobin ＞9%), the age, body mass index, blood lipid, visceral fat area and gonadal hormone of the patients were collected, and the data of the three groups were statistically analyzed. According to testosterone greater than the median (≤4.08 ng/mL) and less than or equa to the median (＜4.08 ng/mL), they were divided into two groups, and the body mass index, visceral fat area, glycosylated hemoglobin, blood lipids and other data between the two groups were statistically analyzed. Results: The level of glycosylated hemoglobin was negatively correlated with high density lipoprotein and testosterone (P＜0.05), and positively correlated with triglyceride, total cholesterol, low density lipoprotein, body mass index, visceral fat area and estradiol (P＜0.05), but not with follicle stimulating hormone, luteinizing hormone and prolactin (P＞0.05). Testosterone was negatively correlated with body mass index, visceral fat area, glycosylated hemoglobin, triglyceride, total cholesterol and low density lipoprotein (P＜0.05), and positively correlated with high density lipoprotein (P＜0.05). Logistic regression analysis showed that glycosylated hemoglobin level, visceral fat area, body mass index and dyslipidemia were risk factors for testosterone reduction. Conclusion: Good blood glucose control can effectively reduce the decrease of testosterone in men, and can effectively promote the body's lipid metabolism; higher testosterone levels also play a positive role in blood glucose and lipid metabolism. Glycosylated hemoglobin, visceral fat area, body mass index, dyslipidemia are risk factors for testosterone reduction.

Objective: To compare the levels of peripheral blood ferritin in patients with acute and chronic brucellosis before and during treatment, and to evaluate the relationship between serum ferritin and inflammatory response of acute and chronic brucellosis and the relationship between treatment outcome of brucellosis. Methods: Thirty-six patients with acute and chronic brucellosis who did not receive antibiotic treatment were collected as the pre-treatment group. Thirty-six patients with acute and chronic brucellosis who were treated with antibiotics for about 10 days were selected as the treatment group. Twenty-five healthy subjects were selected as the control group. Serum ferritin levels in each group were measured by enzyme-linked immunosorbent assay. The differences of serum ferritin levels among groups were statistically analyzed. Results: Before treatment, the level of serum ferritin in acute and chronic phase and control group was respectively different (P＜0.01). The level of ferritin in acute and chronic groups was significantly higher than that in control group (P＜0.01). The level of ferritin in the acute group was significantly higher than that in the chronic group (P＜0.01). During the treatment, the the level of serum ferritin in the acute and chronic phases and the control group was respectively different (P＜0.01). The level of ferritin in the acute and chronic brucellosis groups was significantly higher than that in the control group (P＜0.01). The level of ferritin in acute group was significantly lower than that in chronic group (P＜0.01). The levels of ferritin in acute and chronic brucellosis were significantly lower than those before treatment (P＜0.001). Conclusion: Serum ferritin is related to the degree of inflammatory response of brucellosis, which can be used to monitor the degree of inflammatory response of brucellosis and evaluate the therapeutic effect.

Objective: To investigate the value of pro-gastrin-releasing peptide(Pro-GRP)and neuron-specific enolase (NSE) in the diagnosis and treatment of small cell lung cancer. Methods: Patients who were hospitalized in the Department of Respiratory and Critical Care Medicine of the First Affiliated Hospital of Bengbu Medical College from April 2020 to March 2021 and were first diagnosed with small cell lung cancer (38 patients), non-small cell lung cancer (65 patients) and benign lung disease (51 patients) were included. The clinical general data of the patients were collected and the levels of ProGRP and NSE were detected by electrochemiluminescence to evaluate the clinical diagnosis and treatment value. Results: The levels of serum Pro-GRP and NSE in small cell lung cancer group were significantly higher than those in non-small cell lung cancer group (P＜0.05) and benign lung disease group (P＜0.05). The levels of serum Pro-GRP and NSE in extensive small cell lung cancer group were higher than those in limited small cell lung cancer group (P＜0.05). There was no significant correlation between serum ProGRP level and gender, age and smoking history in patients with small cell lung cancer (P＞0.05). There was no significant correlation between serum NSE level and gender and age history (P＞0.05), but there was a correlation with smoking (P＜0.05). ROC curve analysis showed that the cut-off values of serum Pro-GRP and NSE in the diagnosis of small cell lung cancer were 65.78 pg/mL and 14.84 ng/mL, respectively; the sensitivity, specificity and Youden index of serum Pro-GRP in the diagnosis of small cell lung cancer were 84.21%, 96.1% and 0.803, respectively; the sensitivity, specificity and Youden index of NSE were 68.4%, 96.1% and 0.645, respectively. After 2 cycles of standard chemotherapy, the levels of serum Pro-GRP and NSE in patients with small cell lung cancer after chemotherapy were significantly lower than those before chemotherapy (P＜0.05). Conclusion: Serum Pro-GRP and NSE can be used as tumor markers for the diagnosis and efficacy evaluation of small cell lung cancer.

Objective: To explore the clinical significance of sIgA in alveolar lavage fluid, serum IgA and blood T lymphocyte subsets in patients with chronic obstructive pulmonary disease, pneumonia and healthy people by comparing their differences. Methods: The inpatients from the Department of Respiratory and Critical Care Medicine of Baotou Central Hospital and the health personnel from the Health examination Center of Baotou Central Hospital from December 2018 to March 2020 were collected. There were 29 patients with chronic obstructive pulmonary disease combined with pneumonia (COPD-P group), 26 patients with simple chronic obstructive pulmonary disease (S-COPD group), 27 patients with simple pneumonia (S-P group), and 27 health personnel in the control group (CG group). sIgA and serum levels of IgA, CD4⁺T cells, CD8⁺T cells, absolute number of T lymphocytes, CD4⁺T/CD8⁺T ratio and other data were detected in alveolar lavage fluid. SPSS 20.0 software was used for data analysis. Results: There was no significant difference in serum IgA, T lymphocyte absolute number and CD4⁺T cell percentage among the four groups (P＞0.05). sIgA level, CD4⁺T cell /CD8⁺T cell ratio, and percentage of CD8⁺T cell were significantly different among the four groups (P＜0.05). Conclusion: sIgA content in alveolar lavage fluid was significantly reduced in patients with S-COPD and COPD P, suggesting that the lack of sIgA on the mucosal surface caused mucosal barrier damage. Infection is associated with a lack of sIgA on the mucosal surface, which may be one of the reasons for the increased chance of COPD infection. The level of CD8⁺T cells in the serum of COPD patients increased, and the imbalance of the ratio of CD4⁺T cells /CD8⁺T cells was common in COPD patients, indicating the dysfunction of cellular immunity. In COPD, the ratio of CD4⁺T/CD8⁺T cells was used as one of the indicators to predict the disturbance of the internal cell composition of T lymphocyte subsets, which has a suggestive effect.

Objective: To analyze the clinical features, in-hospital treatment and 12-month outcomes of heart failure inpatients with different age shock indexes (ASI). Methods: A total of 259 heart failure patients aged over 18 years who were admitted in the Second Affiliated Hospital of Baotou Medical College from October 2020 to November 2021 were consecutively included in this study. The baseline data were collected and ASI (age × heart rate/systolic blood pressure) was calculated on the day of admission. Patients were divided into the high ASI (n=129) and low ASI group (n=129). Clinical features of the two groups of patients, differences in hospital treatment and outcomes of 12 months after discharge, and correlation between ASI and the risk for all-cause mortality 12 months after discharge in patients were analyzed. Results: Lower body mass index, systolic blood pressure, diastolic blood pressure and left ventricular ejection fraction value were found in the High ASI group of patients, with thinner left ventricular posterior wall and higher brain natriuretic peptide and white blood cell count. More patients in the high ASI group took β-blockers orally and used inotropes and diuretics intravenously than in the low ASI group (P＜0.05). 12 months after discharge, patients in the high ASI group had more rehospitalizations (34.9%) and all-cause deaths (23.3%), and a higher risk of all-cause death after 12 months. (HR=3.05, 95%CI: 1.25~7.45, P=0.014). Conclusion: Patients in the high ASI and low ASI groups had different clinical characteristics and similar treatments during hospitalization. The risk of all-cause death 12 months after discharge was higher in patients with high ASI than patients with low ASI. ASI was an independent risk factor for long-term prognosis of hospitalized patients with heart failure.

Objective: To investigate the relationship between serum neutrophil extracellular traps (NETs), interleukin-13 (IL-13), T-helper 17 cells /regulatory T cells (Th17/Treg) and acute asthma exacerbation in children. Methods: A total of 128 children with asthma who were treated in Xihua County People's Hospital from September 2019 to July 2021 were selected as the research objects. All selected children were divided into two groups (75 cases in the acute attack group and 53 cases in the remission group) according to the period of asthma attack. 64 healthy children who underwent physical examination during the same period were selected as the reference group. The levels of NETs,IL-13,Th17/Treg and airway inflammation indexes (serum total immunoglobulin E, IgE), fractional exhaled nitric ox (FeNO), sputum eosinophils (EOS) and blood eosinophils (BEOSs) of the three groups were recorded and compared, and the correlation between serum NETs, IL-13, Th17/Treg and airway inflammation and their predictive value for acute exacerbation in children with asthma were analyzed. Results: Comparison of NETs, IL-13, Th17/Treg levels at admission showed that acute attack group ＞remission group ＞ reference group (P＜0.05). Comparison of IgE, FeNO, EOS, BEOS levels at admission showed that acute attack group ＞remission group ＞reference group (P＜0.05). Pearson correlation analysis showed that the changes of serum NETs, IL-13, Th17/Treg levels at admission were positively correlated with IgE, FeNO, EOS, and BEOS (P＜0.05). The predicted AUCs of IL-13 and Th17/Treg levels in the acute exacerbation period of children with asthma were 0.864, 0.838,and 0.789 respectively, and the combined predicted AUC was 0.928 (P＜0.05). Conclusion: NETs, IL-13 and Th17/Treg could be used to evaluate and predict acute exacerbation in children with asthma, which could provide a basis for clinical evaluation of children's disease and airway inflammation, and help clinicians make effective intervention measures.

Objective: To observe the effect of Mongolian medicine total flavones of Dracocephalum moldavica L. (TFDM) on the expression of vascular endothelial growth factor 2 (VEGFR2) in ischemic penumbra of rats after cerebral ischemia-reperfusion, and to explore its effect on angiogenesis. Methods: The middle cerebral artery occlusion (MCAO) reperfusion model of SD rats was prepared by suture method. TFDM 50 mg/kg was administered by intragastric administration. The samples were taken at 1, 3, 7 and 14 days after operation. Immunohistochemical staining was used to observe the distribution of VEGFR2 positive labeled cells and neovascularization in the ischemic penumbra. Western blot was used to detect the expression of VEGFR2 in the ischemic penumbra. Results: Immunohistochemical staining showed that the number of positive cells in the TFDM administration group on the 3rd to 14th day after MCAO reperfusion was significantly higher than that in the model group (P＜0.05), and the expression increased near the microvessels, arterioles and venules. Western blot also showed that the expression of VEGFR2 was up-regulated in TFDM group. Conclusion: TFDM may upregulate the expression of VEGFR2 protein, promote the generation of microvessels in the ischemic penumbra, and improve microcirculation function.