Objective:To detect and evaluate lanthanum, cerium and neodymium in agricultural soil in Baotou suburb, and to provide scientific basis for the development and application of rare earth and the protection and utilization of soil resources in Baotou. Methods: According to the agricultural industry standard of the People's Republic of China, 6 samples from Machi Town and 5 samples from Shidagu Village of Shaerqin Town were randomly collected according to the plum blossom point method, and 19 test samples including standard series were made. The samples were pretreated by microwave digestion instrument, and the contents of lanthanum, cerium and neodymium in soil samples were determined by inductively coupled plasma mass spectrometry. The results were analyzed by Nemero index method. Results: The average content of lanthanum, cerium and neodymium in the soil of Machi Town was 20.45 mg/kg, 39.32 mg/kg and 16.97 mg/kg, respectively. The average content of lanthanum, cerium and neodymium in the soil of Shidagu Village in Shaerqin Town was 23.99 mg/kg, 46.31 mg/kg and 19.87 mg/kg, respectively. The single factor evaluation method was used to evaluate the pollution status of lanthanum, cerium and neodymium in soil, the Pi values of lanthanum, cerium and neodymium in the soil of Shidagu Village, Machi Town and Shaerqin Town were all less than 1. The Nemerow index method was used to evaluate the pollution of metal elements in surface soil, the comprehensive pollution index of land in Machi Town of Baotou City was 0.81, and the comprehensive pollution index of land in Shidagu Village of Shaerqin Town was 0.89, both of which were lower than the comprehensive pollution index of Gannan District. Conclusion: The overall agricultural soil in Shidagu Village and Machi Town, Shaerqin Town, Baotou City is in good condition and is not polluted by rare earth elements lanthanum, cerium and neodymium. Compared with Gannan area, the cleaning status of lanthanum, cerium and neodymium in agricultural soil of Baotou city is higher.

Objective:To explore the clinical characteristics and prognosis of 104 children hospitalized for pediatric neurosurgical diseases in Baotou Fourth Hospital, the First Affiliated Hospital of Baotou Medical College, Baotou Central Hospital and other hospitals from 2018 to 2020. Methods: The clinical data of hospitalized children with pediatric neurosurgery diseases in the above hospitals from January 2018 to December 2020 were retrospectively analyzed. The general situation, disease distribution, disease assessment, diagnosis and treatment methods, discharge GOS score (glasgow outcome scale) and other clinical data were summarized and analyzed. Results: (1) A total of 104 patients were enrolled, with an average age of (8.3±3.9) years. (2) The number of inpatients with cerebrovascular disease was 54 cases (51.90%). The number of hospitalized patients with traumatic brain injury was 50 cases (48.10%). (3) The main cause of hospitalization was trauma, the number of cases was 103 cases (99.04%), and the number of non-traumatic factors was 1 (0.86%). (4) 101 cases (97.16%) were treated with conservative treatment, 3 cases (2.84%) were treated with surgical treatment. (5) Most of the hospitalized children were emergency and critical cases, with a total of 80 cases (76.92%), and 24 cases (23.08%) had general clinical classification. (6) In cerebrovascular disease, epidural hemorrhage was 21 cases (38.90%). In traumatic diseases, 22 cases (44.00%) had head and facial soft tissue injuries. Conclusion: Through the epidemiological analysis of the hospitalized children in the pediatric neurosurgery department of the above hospitals, it is found that the majority of the children admitted to the above hospitals are acute traumatic brain injury. After active professional treatment, the prognosis of the children is good.

Objective:To study the relationship between pre-pregnancy body mass index(BMI) and gestational diabetes(GDM) and its influencing factors in Guangdong. Methods: A total of 488 women who received prenatal physical examinations from January 2015 to October 2020, in Guangdong were selected. 18 women with GDM were divided into the GDM group, and 470 women without GDM into the non-GDM group, and general data such as pre-pregnancy BMI, age, height, parity, waist circumference of all women were statistically analyzed. Results: Compared with the non-GDM group, the pre-pregnancy BMI (P＜0.05) and waist circumference (P＜0.05) in the GDM group were higher than those in the non-GDM group. There were 74 cases of normal weight gaining, 136 cases of moderate weight gaining, 112 cases of mild weight gaining, and 166 cases of severe weight gaining in the 488 pregnant women. There were no cases of GDM among 74 pregnant women within the normal range of weight gaining. There were only 1 case of GDM occurred in the 112 pregnant women with mild weight gaining, which accounted for about 0.89%. There were 4 cases GDM occurred among 136 pregnant women with moderate weight gaining (2.94%), and there were 13 cases GDM occurred among 166 pregnant women with severe weight gaining (7.83%). Logistic regression analysis showed that the BMI of pregnant women (＞26.0 kg/m²), severe weight gaining during pregnancy, waist circumference (＞74.0 cm) were risk factors for pregnant women to develop GDM. After excluding factor of weight gaining during pregnancy, Logistic regression analysis showed that there was no statistical relationship between BMI and waist circumference (＞74.0 cm) with the occurrence of GDM in pregnant women (P＞0.05). Conclusion: Prenatal BMI and waist circumference are independent risk factors for diabetes in pregnant women in Guangdong, and there is a correlation between gestational weight gaining and gestational diabetes, which has important guiding significance for adjusting prenatal BMI and controlling gestational weight gaining.

Objective:To obtain the differential genes of Alzheimer's disease (AD) by bioinformatics methods, and to predict the potential traditional Chinese medicines, components and targets for the treatment of AD. Methods: The AD gene chip data GSE129055 was downloaded from the GEO database. The differentially expressed genes in the data set were screened and the differentially expressed mRNAs were analyzed by gene ontology function (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The target genes of miRNAs were predicted by TargetScan database and the miRNA-mRNA network was constructed. The top 50% mRNA of Degree value in miRNA-mRNA network was imported into Coremine Medical platform to screen potential therapeutic Chinese medicine. The TCMSP platform was used to screen the components and targets of potential Chinese medicines acting on differentially expressed mRNAs. The two were combined to construct a drug-component-mRNA-miRNA co-expression network for Chinese medicine treatment of AD and molecular docking verification was performed. Results: A total of 178 differentially expressed mRNAs and 96 differentially expressed miRNAs were screened in the GSE129055 data set. Through screening, it was found that the commonly used traditional Chinese medicines acting on differentially expressed mRNAs were ginseng, salvia miltiorrhiza, scutellaria baicalensis, cordyceps sinensis, schisandra chinensis, etc. The key active ingredients were levoshansophorin and β-sitosterol. The key targets were μ-opioid receptor gene (OPRM1) and 5-hydroxytryptamine receptor subunit 3A positive inhibitory neuron (HTR3A), and the key miRNAs were miR-9-3p and miR-3607-3p. The results of molecular docking showed that the key active components could bind to the key targets and showed good affinity. Conclusion: The expression of multiple genes in hippocampus of AD rats is different. At the same time, traditional Chinese medicine has the characteristics of multi-component and multi-target in the treatment of AD. It can play a neuroprotective role by neuropeptides, biogenic amines and reducing neuroinflammation.

Objective:To investigate the regulatory effect of hypoxia inducible factor-1α (HIF-1α) on macrophages in the thymus tissue of macrophage cell line RAW264.7 and collagen induced arthritis (CIA) mice. Methods: (1) RAW264.7 macrophages were divided into blank group, negative control group, HIF-1α-siRNA group and overexpression HIF-1α group. RT-PCR and Western blot (WB) were used to detect the expression of HIF-1α at mRNA and protein levels. The relative expression of CD206 and Arg in the four groups of cells was detected by RT-PCR. The proportion of CD16/32⁺ and CD206⁺ macrophages was detected by flow cytometry. (2) CIA mouse model was established by bovine type Ⅱ collagen in DBA/1 mice. The experiment was divided into blank group, model group, negative control group, HIF-1α-siRNA group and overexpressed HIF-1α group. The proportion of F4/80⁺CD16/32⁺M1 and F4/80⁺CD206⁺M2 macrophages in mouse thymus was detected by flow cytometry. HE staining was used to observe the pathological changes of ankle joint in mice. Results: (1)Silencing HIF-1α could decrease the proportion of F4/80⁺CD16/32⁺M1 macrophages in mouse thymus tissue, and increase the proportion of F4/80⁺CD206⁺M2 macrophages in RAW264.7 cell line and mouse thymus tissue. Overexpression of HIF-1α had the opposite effect. (2)The synovium of the ankle joint of CIA mice was proliferated, with a large number of cell infiltration. While, HIF-1α-siRNA could reduce the infiltration of lymphocytes in the synovial tissue and reduce the proliferation of the synovial membrane. Overexpression of HIF-1α has the opposite effect. Conclusion: HIF-1α can regulate the differentiation of macrophages in RAW264.7 cell line and the transformation of M1 and M2 macrophages in thymus tissue of CIA mice, thus affecting the progress of joint inflammation in CIA mice, indicating that HIF-1α can mediate the disease process of CIA mice by regulating the polarization of macrophages.

Objective:To investigate the protective effect of resveratrol (RES) on liver injury induced by alcohol dependence in rats. Methods: According to the random number table method, SD rats were divided into drinking group and blank control group. The rats in the drinking group were given two bottles of free drinking 20% alcohol every other day to establish an alcohol dependence model, and the rats in the blank control group were given two bottles of free drinking water. After successful modeling, the rats in the drinking group were randomly divided into alcohol dependence model group (AD), RES low, medium and high dose administration groups (Low, Middle, High). After treatment, the activities of catalase (CAT), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and super oxide dismutase (SOD) in liver tissues were detected. Western Blot was used to detect the protein expression of p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK), p-P38 MAPK and p-JNK. Results: Compared with the blank control group, the activity of MDA in liver tissue of AD group was increased (P＜0.05), the activities of CAT, GSH-PX and SOD were decreased (P＜0.05), and the protein expression of p-P38 and p-JNK was significantly increased (P＜0.05). Compared with the AD group, the activity of MDA in the middle and high dose RES groups was significantly decreased (P＜0.05), the activities of CAT, GSH-PX and SOD were significantly increased (P＜0.05), and the expression of p-P38 and p-JNK protein was significantly decreased (P＜0.05). Conclusion: RES can improve the liver injury induced by alcohol dependence in rats, and may reduce the oxidative stress injury of liver tissue by inhibiting the phosphorylation of JNK and P38 proteins in JNK/P38 signaling pathway, and play a protective role in the liver of alcohol dependent rats.

Objective:To investigate the molecular mechanism of protocatechuic acid inhibiting the progression of intracranial aneurysms by inhibiting IL-6/JAK1/STAT5 signaling pathway. Methods: The rat model of intracranial aneurysm was constructed and divided into NC group (blank control group), intracranial aneurysm group (CA group) and intracranial aneurysm model+protocatechuic acid group (CA+PCA group). The inner diameter of intracranial vascular wall in each group was detected by imaging, and the vascular endothelium in each group was detected by HE staining. The expression of IL-6, JAK1 and STAT5 in peripheral blood of each group was detected by ELISA, and the expression levels of IL-6, JAK1 and STAT5 in tissue samples of each group were detected by RT-PCR and WB. Results: Compared with the NC group, the thickness of the vascular wall in the CA group was significantly increased from the eighth week (P＜0.05), while the thickness of the vascular wall in the CA+PCA group was lower than that in the CA group. The results of HE staining showed that compared with the NC group, the number of macrophages in the vascular endothelium of the CA group increased, while the number of macrophages in the vascular endothelium of the CA+PCA group decreased compared with the CA group. ELISA experiments showed that the expression of IL-6, JAK1 and STAT5 in CA group was higher than that in NC group, while the expression of IL-6, JAK1 and STAT5 in CA+PCA group was lower than that in CA group. RT-PCR and WB results were the same as ELISA results (P＜0.05). Conclusion: Protocatechuic acid inhibits the progression of intracranial aneurysms by inhibiting the IL-6/JAK1/STAT5 signaling pathway.

Objective:To explore future research hotspots and to provide reference for researches on the field of PIK3CA gene-related colorectal cancer in China by studying related literatures in China. Methods: Literatures related to PIK3CA gene-related colorectal cancer were retrieved from SinoMed, CNKI, Wanfang Data and VIP databases from 2009 to 2022 respectively. CiteSpace 6.1 and NoteExpress software were used to analyze the publication years, Journals, authors, institutions and key words. Results: A total of 105 Chinese literatures were included. The number of published papers is on the rise, and the published Journals were scattered. The core team mainly includes Binzhou Medical College and Chinese Academy of Medical Sciences. High frequency keywords are colorectal cancer and gene mutation. The maximum cluster is prognosis. Conclusion: The research on colorectal cancer related to PIK3CA gene has just started and will be developed rapidly in the future. At present, independent team research on this topic from different institutions is the main force in China, inter-institutional cooperation is less found. Researches mainly focus on colorectal cancer oncogenes, research techniques and advanced colorectal cancer.

Objective:To investigate expressions of phosphoribosyl pyrophosphate synthetase 2(PRPS2), cyclin dependent kinase 6(CDK6), retinoblastoma(Rb) in different stages of cervical intraepithelial lesions and cervical cancer and their correlation with human papilloma virus(HPV) infection, aiming to determine the relationship of different factors with the evolution of cervical cancer and their possible regulatory mechanism. Methods: A total of 130 patients in the Department of Pathology, the First Affiliated Hospital of Bengbu Medical College from January 2018 to December 2018 were selected, including 40 cases of cervical cancer(cervical cancer group), 40 cases of high-grade squamous intraepithelial lesion(HSIL) of cervix(HSIL group), 30 cases of low-grade squamous intraepithelial lesion(LSIL) of cervix(LSIL group) and 20 cases of chronic inflammation(the control group). The expression of PRPS2, CDK6 and Rb in different grades of cervical lesions were detected using immunohistochemistry. HPV infection data of each case was collected, and the HPV infection condition and the correlation of each factor were analyzed. Results: The positive rates of PRPS2, CDK6, and Rb in the control group, LSIL group, HSIL group and cervical cancer group were (10.0%, 40.0%, 77.5%, 87.5%), (10.0%, 36.7%, 50.0%, 82.5%), and (80.0%, 63.3%, 32.5%, 10.0%) respectively, (χ²=44.02, P＜0.05; χ²=31.81, P＜0.05; χ²=36.08, P＜0.05). There were significant differences in the expression levels of PRPS2, CDK6 and Rb between groups. The correlation analysis result indicated that protein expressions of PRPS2, CDK6 and Rb were correlated. There were differences in the HPV infection rate between groups. The expression of each factor was associated with HPV infection. Conclusion: The gradual evolution of cervical cancer was positively correlated with the overexpression of PRPS2 and CDK proteins, and related to the loss of Rb protein expression, which may accelerate transformation of cell cycle by cooperating with HPV through the CDK6-Rb-E2F pathway to promote tumor formation.

Objective:To study the correlation between atrial fibrillation (AF) and micro RNA-21 (miRNA-21) through clinical data, in order to provide reference value for clinical diagnosis and treatment of AF. Methods: A total of 142 patients who were hospitalized in the Department of Cardiology of a tertiary hospital in Tianjin from September 2022 to December 2023 were collected. According to the results of 12-lead surface electrocardiogram, 70 patients who met the conditions of ′P wave disappeared, replaced by small f waves of different sizes and shapes, and RR interval was absolutely irregular′ were included in the AF group, and the remaining 72 patients were included in the control group. Plasma miRNA-21 and echocardiography were compared between the two groups. Results: Compared with the control group, the dosage of anticoagulants such as rivaroxaban, dabigatran etexilate, warfarin and amiodarone in AF group was significantly higher than that in control group (P＜0.05), the level of low density lipoprotein cholesterol (LDLc) was lower (P＜0.05), the diameter of left atrium was larger (P＜0.05), and the expression of miRNA-21 in plasma was higher (P＜0.05). Logistic regression analysis showed that the occurrence of AF was related to left atrial diameter and miRNA-21 indexes (P＜0.05). Conclusion: The occurrence of AF may be correlated with the expression of miRNA-21 in plasma and the size of left atrium.

Objective:To investigate the predictive value of triglyceride glucose product index (TyG) combined with systemic immune inflammation index (SII) on the degree of coronary artery stenosis in patients with coronary heart disease (CAD) complicated with type 2 diabetes mellitus (T2DM). Methods: From December 2022 to December 2023, 246 patients with T2DM who were admitted to the Department of Cardiology of Baotou Central Hospital of Inner Mongolia for suspected CAD and underwent coronary angiography during hospitalization and whose results met the diagnostic criteria of CAD were selected as subjects. According to the median Gensini score method, they were divided into low stenosis group (≤23 scores, n=126) and high stenosis group (＞23 scores, n=120). Multivariate logistic regression analysis was used to analyze the correlation between TyG index, SII and other indicators and the degree of coronary artery stenosis in CAD patients with T2DM, and the combined equation was constructed, the ROC curves of single and multiple indicators were drawn, the area under the curve was reported to further evaluate the predictive value of TyG index, SII and the combined coefficient of the two for the degree of coronary stenosis in CAD patients with T2DM. Results: Multivariate Logistic regression analysis showed that smoking history, history of hypertension, TyG index and SII were independent risk factors for coronary artery stenosis in CAD patients with T2DM. The combined coefficient of TyG index and SII was used to draw the area under the ROC curve AUC=0.723 [95% CI (0.661-0.785), P＜0.05], suggesting that the predictive ability was good. Conclusion: TyG index and SII are independent risk factors for coronary artery stenosis in CAD patients with T2DM. TyG index combined with SII can better predict the degree of coronary artery stenosis in CAD patients with T2DM.

Objective:To investigate the predictive value of combined detection of serum irisin, matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) for diabetic retinopathy (DR) in type 2 diabetes mellitus (T2DM). Methods: A total of 200 patients and healthy subjects who were hospitalized in the Inner Mongolia Autonomous Region People's Hospital from November 2022 to November 2023 were selected. According to the DR process, they were divided into four groups. Serum Irisin, MMP-9 and VEGF were detected by ELISA. Correlation analysis, ROC curve analysis and multiple regression analysis were performed. Results: The levels of irisin in type 2 diabetes mellitus without retinopathy (NDR) group, non-proliferative diabetic retinopathy (NPDR) group and proliferative diabetic retinopathy (PDR) group were significantly lower than those in the control group, and the levels of MMP-9 and VEGF were significantly higher than those in the control group, the difference were statistically significant (P＜0.05). Spearman correlation analysis showed that Irisin was negatively correlated with MMP-9 and VEGF (P＜0.05). Logistic regression analysis showed that Irisin, MMP-9 and VEGF were risk factors for DR. ROC curve analysis showed that the diagnostic value of Irisin, MMP-9 and VEGF in the diagnosis of DR was higher than that of single factor. Conclusion: Irisin, MMP-9 and VEGF are involved in the pathological process of DR, and Irisin has a predictive effect on DR.

Objective:To study the efficacy of ultrasound-guided percutaneous transhepatic gallbladder fistula combined with laparoscopic cholecystectomy in the treatment of acute cholecystitis in the elderly. Methods: A total of 60 elderly patients with acute severe calculous cholecystitis admitted to Puyang People's Hospital from June 2022 to December 2023 were randomly divided into ultrasound-guided percutaneous transhepatic gallbladder fistula combined with laparoscopic cholecystectomy group (observation group) and laparoscopic cholecystectomy group (control group), with 30 patients in each group. The VAS score, intraoperative blood loss and operation time, the first time to get out of bed after operation, hospitalization time, postoperative complications and perioperative endocrine hormones, cortisol and adrenaline levels were observed and compared between the two groups. Results: The VAS score of the observation group was lower than that of the control group (P＜0.05). The intraoperative bleeding in the observation group was less than that in the control group, the first time of getting out of bed after operation and hospitalization time in the observation group were shorter than those in the control group, the incidence of postoperative complications in the observation group was less than that in the control group (P＜0.05). The levels of cortisol and epinephrine in the observation group were lower (P＜0.05). Conclusion: Ultrasound-guided percutaneous transhepatic gallbladder puncture and fistulation in patients with laparoscopic cholecystectomy can better reduce postoperative pain and surgical stress response, and effectively reduce the occurrence of complications and improve the quality of postoperative rehabilitation.

Objective:To explore the effect of personalized health education and nursing on the prognosis of patients with acute coronary syndrome. Methods: A total of 160 elderly patients with acute coronary syndrome hospitalized in our hospital from August 2017 to February 2020 were selected. The patients were randomly divided into treatment group and control group with 80 cases in each group, both groups were given routine examination and treatment. During hospitalization, the control group was given traditional health education, while the treatment group was given personalized health education and nursing on this basis. The compliance, adverse reactions, cardiac function and psychological changes were compared between the two groups. Results: The compliance of the treatment group and the control group during hospitalization was 97.5% and 88.8% respectively. The compliance of the treatment group was higher than that of the control group (P＜0.05). The total incidence of adverse reactions in the treatment group was lower than that in the control group(P＜0.05). LVEDV and LVESV in the two groups after nursing were lower than those before nursing(P＜0.05), and LVEDV and LVESV in the treatment group after nursing were lower than those in the control group(P＜0.05). The scores of anxiety and depression in the two groups after nursing were lower than those before nursing(P＜0.05), and the scores of anxiety and depression in the treatment group after nursing were lower than those in the control group(P＜0.05). Conclusion: The application of personalized health education and nursing care in patients with acute coronary syndrome can promote the improvement of patients′ compliance, reduce the adverse reactions of patients in hospital, alleviate psychological barriers, and thus be conducive to the improvement of cardiac function.

Objective:To explore the clinical effect of emotional nursing in patients with menstrual disorders and hypertension and its influence on patients′ emotions and body. Methods: A total of 102 patients with menstrual disorders and hypertension who were admitted to the Third Affiliated Hospital of Zhengzhou University from January 2019 to January 2021 were selected as the research objects. All selected patients were divided into the control group (51 cases) and the observation group (51 cases) using the random number table method. All patients were given symptomatic treatment and routine nursing. The observation group were given TCM emotional nursing on the basis of symptomatic treatment and routine nursing. Then the nursing effect of the two groups were compared. Results: The effective rate of blood pressure control in the observation group was higher than that in the control group (P＜0.05), the SAS (self-rating anxiety scale) and SDS (self-rating depression scale) scores in the observation group were lower than those in the control group (P＜0.05), and the health belief score in the observation group was higher than that in the control group (P＜0.05). The treatment compliance score in the observation group was better than that of the control group, and the difference was statistically significant (P＜0.05). Conclusion: Emotional nursing is an effective way in treating patients with menstrual disorders and hypertension, and the nursing effect is remarkable. It could help to regulate the negative emotions and blood pressure, improve health beliefs and treatment compliance of patients with menstrual disorders and hypertension, which is worthy of popularization and application.

Objective:To explore the application effect of 4C model-oriented extended nursing service in patients with cardiac cancer after surgery. Methods: A total of 88 patients with cardiac cancer who were treated in our hospital from December 2019 to July 2021 were selected as the research objects, and divided into the control group and the experimental group using sealed envelope randomization with 44 cases in each group. Both groups were treated with surgery, the control group was given routine nursing, and the experimental group was given extended nursing services oriented by the 4C model. The scores of self-rating anxiety scale (SAS), self-rating depression scale (SDS), self-efficacy scale (GSES), quality of life scale (QOLS), incidence rate of complication and nursing satisfaction were compared between the two groups. Results: Compared with the control group, the SAS and SDS scores of the experimental group were decreased after intervention(P＜0.05). After nursing intervention, the GSES and QLQ-C30 scores of the experimental group were lower than those in the control group (P＜0.05). The incidence rate of complications in the experimental group was 4.55%, which was lower than that of the control group(18.18%) (P＜0.05). Nursing satisfaction in the experimental group was 97.93%, which was higher than that in the control group (81.82%) (P＜0.05). Conclusion: Extended nursing service oriented by 4C model could improve the self-efficacy, reduce negative emotions, reduce the incidence rate of postoperative complications, and improve the quality of life and nursing satisfaction of patients with cardiac cancer after surgery.