Objective: To screen a bacterium with high arsenic tolerance and provide high-quality strains for arsenic removal in arsenic contaminated environments. Methods: As(Ⅲ)-tolerant bacteria were isolated from soil and water samples collected from arsenic-containing areas, and their growth characteristics were studied under different temperatures, pH values and arsenic concentrations, a mathematical model was established to study the specific adsorption and arsenic removal laws of bacterial strains on As(Ⅲ). Results: The strain NSSbac-2 with high tolerance to As(Ⅲ) was screened out, which was Pseudomonas mendocina. The optimum growth conditions were temperature 37 ℃, pH=6, phosphate concentration 40 mg/L, and could survive under the condition of As(Ⅲ) concentration 600 mg/L; the kinetic model showed that the strain conformed to the surface adsorption law of As(Ⅲ). Conclusion: The strain NSSbac-2 showed excellent tolerance to As(Ⅲ).The kinetic model of As(Ⅲ) removal belongs to surface adsorption, which has the potential for analytical reuse and has high application value in treating arsenic-contaminated environments. It provides more scientific basis for further use of microorganisms to repair arsenic pollution and realize the industrialization of microbial treatment of arsenic pollution.

Objective: To analyze the risk factors for recurrence of lumbar disc herniation after percutaneous transforaminal endoscopic discectomy. Methods: From January 2018 to October 2022, a total of 2 123 patients with lumbar disc herniation(LDH) who underwent percutaneous transforaminal endoscopic discectomy in the Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Qujing Hospital Affiliated to Yunnan University of Traditional Chinese Medicine, Tongxiang Hospital of Traditional Chinese Medicine and Xuanhan County People's Hospital were analyzed. The preoperative general conditions (gender, age, body mass index, herniated segment, herniated type, degree of disc degeneration), surgical conditions (endoscopic type, annulus fibrosus treatment, nucleus pulposus removal volume, lateral recess decompression, preoperative endplate inflammation) were collected, and the recurrence was followed up after operation. The clinical data of the two groups were compared, and the risk factors of postoperative recurrence were evaluated by univariate and multivariate logistic analysis. Results: There were significant differences in the type of disc herniation, annulus fibrosus treatment, degree of disc degeneration, volume of nucleus pulposus removal, decompression of lateral recess and endplate inflammation between the recurrent group and the non-recurrent group (P＜0.05). Univariate logistic regression analysis showed that the influencing factors of recurrence of lumbar disc herniation after percutaneous transforaminal endoscopic discectomy may include the type of herniation, the suture of annulus fibrosus, the degree of disc degeneration, the volume of nucleus pulposus removal, the adequacy of lateral recess decompression, and the presence of endplate inflammation (P＜0.05). Multivariate logistic regression analysis showed that annulus fibrosus treatment, degree of intervertebral disc degeneration, volume of nucleus pulposus removal, lateral recess decompression, and endplate inflammation (P＜0.05) were the influencing factors of recurrence of lumbar disc herniation after percutaneous transforaminal endoscopic discectomy. Conclusion: Improper treatment of annulus fibrosus, high degree of intervertebral disc degeneration, incomplete removal of nucleus pulposus, inadequate decompression of lateral recess and endplate inflammation are the influencing factors of recurrence of lumbar disc herniation after percutaneous transforaminal endoscopic discectomy.

Objective: To study the status quo and correlation of medication compliance in patients with rheumatoid arthritis (RA) at home, in order to provide a theoretical basis for improving patients' medication compliance. Methods: A total of 110 RA patients receiving home-based care from the second affiliated hospital of Shandong first medical university were selected as study subjects between March 2024 and June 2024. Assessments were conducted using the Chinese version of the Morisky Medication Adherence Questionnaire-8 (MMAS-8), the Health Assessment Questionnaire (HAQ), and a self-designed basic information survey form. Results: The score of medication compliance of RA patients at home was (4.55±1.63), there were 0 patient (0%) with good medication compliance, 35 patients (31.82%) with moderate medication compliance, and 75 patients (68.18%) with poor medication compliance, the medication compliance was 31.82%. The HAQ score was (10.75±11.84), which was the middle level of the health assessment scale. The health assessment score of RA patients at home was negatively correlated with medication compliance. Age, education level, duration of morning stiffness and health status were the influencing factors of medication compliance. Conclusion: The medication compliance of RA patients at home is poor. Medical staff should take into account the difference of age and education level of patients in clinical and continuous nursing work, and pay attention to the influence of medication compliance on morning stiffness and health status, and formulate individualized health education and chronic disease management strategies according to the patient's condition, so as to improve the medication compliance of RA patients at home.

Objective: To explore the correlation between nutritional status, chemotherapy adverse reactions, and quality of life in gastric cancer patients undergoing chemotherapy. Methods: A total of 300 gastric cancer chemotherapy patients admitted to our hospital from February 2022 to February 2024 were selected as the research subjects. The nutritional status was evaluated using the Nutrition Risk Screening Scale (NRS2002), which was divided into a normal nutrition group (NRS2002<3, n=157 cases) and a nutrition risk group (NRS2002≥3, n=143 cases). Compare the nutritional indicators, chemotherapy adverse reactions, quality of life, and correlation between two groups. Results: The results showed that the levels of hemoglobin (15.86±2.65) g/L, prealbumin (256.43±9.11) mg/L, transferrin (2.47±0.33) g/L, and lymphocyte count (3.05±0.74) × 10⁹/L in the normal nutrition group were higher than those in the nutrition risk group. The grade of chemotherapy adverse reactions was better than that in the nutrition risk group, and the scores of social function (87.93±3.15), emotional function (88.45±3.24), role function (90.51±3.06), physical function (91.23±3.15), and cognitive function (92.03±3.29) were all higher than those in the nutrition risk group (P＜0.05); Pearson correlation analysis showed a negative correlation between nutritional status and chemotherapy adverse reactions, and a positive correlation (P＜0.05) with various dimensions of the Quality of Life Scale (QLQ-30) for cancer patients. Conclusion: Patients with gastric cancer undergoing chemotherapy have a higher risk of malnutrition, which is negatively correlated with chemotherapy adverse reactions and positively correlated with quality of life. Clinical attention should be paid to this and effective intervention measures should be taken to reduce chemotherapy adverse reactions and improve quality of life.

Objective: To study the core components and mechanism of sea buckthorn flavonoids anti-atherosclerosis (anti-AS) by network pharmacology and molecular docking technology. Methods: The active components and targets of Sea buckthorn were searched by TCMSP database, AS targets were collected by Genecards and OMIM database, intersection targets were obtained by Venny 2.1, and PPI networks were mapped by Cytoscape 3.7.2. GO function analysis and KEGG pathway enrichment analysis were performed on the selected targets using Metascape platform. Finally, AutoDock and PyMOL software were used for molecular docking. Results: 11 active ingredients, 224 targets and 80 intersection targets with AS were selected. A total of 5 242 items were identified by GO functional analysis, and 234 pathways were enriched by KEGG pathway analysis, among which fluid shear stress and atherosclerosis pathway, PI3K-Akt signaling pathway and VEGF signaling pathway were key pathways. Molecular docking results showed that the main active components of sea buckthorn quercetin, isorhamnetin and stigasterol had good docking with the target IL1β, PPARG, ADRB2 and NOS3. Conclusion: Quercetin and isorhamnetin in sea buckthorn may act on IL1β, PPARG, ADRB2, NR3C1 and NOS3 targets of fluid shear stress and atherosclerosis pathway, PI3K-Akt signaling pathway and VEGF signaling pathway, thus, it can achieve anti-AS effect by anti-inflammation, regulating oxidative stress, lipid metabolism disorders, and repairing vascular damage, etc., which provides a theoretical basis for subsequent basic research.

Objective: To explore the pattern of serum metabolic disorders in patients with immunoglobulin anephropathy (IgAN) from the perspective of metabolism through non-targeted metabolomics, and to analyze the important metabolites involved in the occurrence and development of IgAN. Methods: Non-targeted metabolomics analysis was conducted on serum samples from 15 patients with IgAN and 15 healthy controls from the First Affiliated Hospital of Baotou Medical College to screen for differential metabolites. Pathway analysis was also performed to reveal the critical metabolic pathways in IgAN. Additionally, the correlations between IgAN metabolites, as well as between metabolites and clinicopathological features were explored to clarify the significance of metabolites in IgAN. Results: (1) There were significant differences in serum metabolites between IgAN patients and healthy controls. Twenty key metabolites including amino acids, uremic toxins and fatty acids, as well as five differential metabolic pathways including tryptophan metabolism, phenylalanine and tyrosine metabolism, citric acid cycle, ketone body metabolism and carnitine synthesis pathway were found, which were helpful to further understand the metabolic network of IgAN. (2)The metabolic pathway significantly associated with IgAN was the metabolism of aromatic amino acids (ie, phenylalanine, tyrosine and tryptophan). (3) There was a significant correlation between differential metabolites and clinical indicators of IgAN patients. The changes of metabolites were related to segmental glomerulosclerosis and increased mesangial cells. Conclusion: The changes of serum metabolites in IgAN patients are involved in the occurrence and development of IgAN.

Objective: To explore the pharmacological mechanism and biological pathway of triptolide (TP) in the treatment of osteosarcoma (OS). Methods: The target genes and mediated signaling pathways of TP for OS were analyzed by whole genome sequencing, topological analysis, multiple public databases as well as in vitro experiments and clinical tissues. Results: 100 nM TP could effectively inhibit the proliferation and viability of U2OS cells, inhibit cell migration and invasion, and promote apoptosis of U2OS cells (P＜0.05). The mRNA and protein expressions of NOC3 like DNA replication regulator(NOC3L)、DEAH-Box Helicase 15(DHX15)and WD repeat domain 3(WDR3) in OS and normal tissues were significantly increased by RT-qPCR and western blotting (P＜0.05), and the results showed that NOC3L, DHX15 and WDR3 were significantly increased in OS tissues (P＜0.05). After U2OS cells were treated with 100 nM TP for 24 h, RT-qPCR and Western blot results showed that the mRNA and protein expression of NOC3L, DHX15 and WDR3 were significantly decreased (P＜0.05). In addition, cell immunofluorescence labeling confirmed that 100 nM TP could effectively inhibit the expression of NOC3L, DHX15 and WDR3. The enrichment results of TP signaling pathway were mainly in Wnt signaling pathway. Western blot analysis (with GAPDH as internal reference) showed that the expression of GSK-3β and p-GSK-3β increased, and the expression of β-catenin and β-catenin (qSer) decreased (P＜0.05). Conclusion: TP inhibits OS progression by suppressing NOC3L, DHX15 and WDR3 expression and Wnt signaling pathway.

Objective: To explore whether SPH3127, a novel renin inhibitor, can protect human umbilical vein fusion cell line EA.hy926 injury induced by angiotensin Ⅱ (AngⅡ) by inhibiting pyroptosis. Methods: The EA.hy926 in logarithmic growth phase was intervened with AngⅡ to construct the hypertension model at the cellular level in vitro. The control group, model group, SPH3127 group and pyroptosis inhibitor MCC950 group were set up respectively. The cell viability was detected by CCK8 method and the cell survival rate was calculated. Cell scratches were used to determine migration; nitrate reductase method was used to detect the content of nitric oxide (NO) in medium of each group. The contents of endothelin-1 (ET-1), interleukin-1β (IL-1β) and IL-18 in medium of each group were detected by ELISA. Western blot was used to detect the protein expression of pyroptosis-related substances NLR family pyrin domain containing 3 (NLRP3), cysteine-requiring aspartate protease-1 (Caspase-1) and N-terminal of pyroptosis protein Gasdermin D (GSDMD-N) in EA.hy926 of each group. Results: Compared with the control group, the survival rate, healing rate and NO content of EA.hy926 cells in the model group were decreased (P＜0.05). The contents of ET-1, IL-1β, IL-18 and the expression levels of NLRP3, Caspase-1 and GSDMD-N protein were increased (P＜0.05). Compared with the model group, the cell survival rate, healing rate and NO content in the SPH3127 group and the MCC950 group were increased (P＜0.05). The contents of ET-1, IL-1β, IL-18 and the expression levels of NLRP3, Caspase-1 and GSDMD-N were decreased (P＜0.05). Compared with MCC950 group, the cell survival rate, healing rate, NO, IL-1β, IL-18 content and NLRP3, Caspase-1, GSDMD-N protein expression levels in SPH3127 group were increased (P＜0.05). ET-1 content decreased (P＜0.05). Conclusion: SPH3127 can effectively improve the survival rate of EA.hy926 cells, reduce the inflammatory response by inhibiting the pyroptosis pathway, and play a protective role in EA.hy926 injury induced by AngⅡ.

Objective: To investigate the application value of combined detection of serum C-reactive protein (CRP), procalcitonin (PCT), blood routine neutrophil count (NC) and neutrophil to lymphocyte ratio (NLR) in the early differential diagnosis of bacterial infectious diseases. Methods: The clinical data, CRP, PCT, NC and NLR of 335 patients with chemotherapy fever who were treated in the Department of Internal Medicine and Department of Thoracic Surgery of Jiangsu Cancer Hospital from August 2022 to September 2023 were collected. Analyze the levels of CRP, PCT, NC,and NLR among the bacterial infection group, fungal infection group, and non-infection group, and evaluate the value of each indicator in the differential diagnosis of fever patients. Results: There were 66 cases of positive bacteria and 34 cases of positive fungi in the specimens of 335 patients with cancer fever, with a positive rate of 29.9%. The proportion of Candida albicans was the highest, followed by Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. There was no significant difference in age, NLR and pathological type between bacterial infection group, fungal infection group and non-infection group (P＞0.05), but there was significant difference in gender and CRP (P＜0.05). There were significant differences in PCT and NC between the bacterial infection group and the non-infection group (P＜0.05). The serum PCT values of the three groups were (6.11±16.14) ng/mL, (1.87±4.23) ng/mL and (2.32±13.19) ng/mL, respectively, which were higher than the normal reference value of 1.5 ng/mL. The CRP values were (81.56±69.20) mg/L, (64.06±60.27) mg/L and (60.51±60.07) mg/L, respectively, which were higher than the normal reference value of 10 mg/L. PCT and CRP had high sensitivity in diagnosing CRP of bacterial infectious fever and non-bacterial infectious fever in cancer patients, while PCT had the highest specificity, and the area under the curve (AUC) was 0.575 and 0.628, respectively. Conclusion: Combined with the characteristics of PCT and CRP serum markers, combined detection can identify the causes of fever in patients with lung cancer chemotherapy, reflect the degree of disease progression in patients with bacterial infectious diseases, and guide clinical rational drug use.

Objective: Assessment of thromboela-stography (TEG) as a monitoring approach for coagulation changes and as a predictor of treatment efficacy in the management of slowly progressive ACLF with DPMAS combined with PE. Methods: A total of forty-nine patients with slowly progressive acute liver failure treated with DPMAS combined with PE in the General Hospital of Ningxia Medical University were collected. TEG and routine coagulation parameters were assessed before and after treatment, and relevant clinical data and coagulation indices,including prothrombin time(PT)、activated partial thromboplastin time(APTT)、international normalized ratio(INR)、plasma fibrinogen(FIB) were collected for statistical analysis. Pearson's method was applied to explore potential correlations between TEG-derived metrics and traditional coagulation indicators. In addition, ROC analysis was conducted to determine the capacity of TEG parameters, both alone and in combination, to predict clinical outcomes in ACLF patients. Results: Compared with pre-treatment levels, patients treated with DPMAS combined with PE showed significant improvements in clinical characteristics and laboratory indicators (P＜0.05) . TEG test results showed a significant decrease in R-value and a significant increase in coagulation index (P=0.001); conventional coagulation tests PT, APTT, INR, and FIB indexes improved significantly, with statistical significance (all P＜0.01). R was found to be positively linked to PT, APTT, and INR, while K showed a negative correlation with PLT and FIB, with all relationships reaching statistical significance (P＜0.05). The AUC of TEG parameters for predicting patient prognosis was significantly higher than that of single-item evaluation(P＜0.01), with a sensitivity of 75.85% and a specificity of 92.17%. Conclusion: In patients with ACLF receiving DPMAS combined with PE, dynamic coagulation profiles assessed by TEG were more reflective of clinical status than conventional indicators and exhibited superior predictive capability regarding outcomes.

Objective: To explore the difference in the effect of radiofrequency combined with magnetoelectric stimulation on pelvic floor function after total hysterectomy in perimenopausal/menopausal patients that compared with single radiofrequency therapy. Methods: Perimenopausal/menopausal patients who underwent total hysterectomy in the Second Affiliated Hospital of Southern Anhui Medical College from January 2022 to November 2023 were divided into control group and experimental group according to the difference of pelvic floor treatment after operation, with 50 cases in each group. The patients in the control group were treated with radio frequency therapy and pelvic floor muscle exercise after operation, while the patients in the experimental group were treated with radio frequency combined with magnetoelectric stimulation and pelvic floor muscle exercise after operation. The patients in the two groups were compared under different interventions in a single-blind and controlled way. Results: After treatment, the pelvic floor muscle strength, POP-Q score and quality of life in the experimental group were better than those in the control group, and the difference was statistically significant (P＜0.05). Conclusion: The intervention of radiofrequency combined with magnetoelectric stimulation for patients after perimenopausal/menopausal hysterectomy can significantly enhance the contractility of pelvic floor muscles, thus improving and preventing the occurrence of pelvic floor dysfunction and improving the quality of life, so it has a good clinical application prospect.

Objective: To observe the effect of immunosuppressant combined with platinum-containing chemotherapy on tumor markers and 3-year survival rate of patients with advanced non-small cell lung cancer (NSCLC). Methods: The clinical data of NSCLC patients in Xinyang Central Hospital from October 2021 to November 2023 were retrospectively selected, and 59 patients treated with platinum-based chemotherapy + sintilimab were selected as the observation group, and 59 cases were selected as the control group according to the matching principle. A total of 118 cases were selected as the samples of this study. The overall response rate (ORR), disease control rate (DCR), carbohydrate antigen 125 (CA125), cytokeratin 19 fragment antigen 21-1 (CYFRA21-1), basic fibroblast growth factor (bFGF), immune function, carcinoembryonic antigen (CEA), Karl Fischer score (KPS), vascular endothelial growth factor (VEGF), toxicity and side effects, and 3-year survival rate were compared between the two groups. Results: After treatment, the DCR and ORR in the observation group were higher than those in the control group (P＜0.05). Compared with the control group, the levels of CYFRA21-1, CEA, bFGF, CA125, and VEGF were lower in the observation group after treatment (P＜0.05). The CD4⁺/CD8⁺, CD3⁺, KPS score, and CD4⁺ levels were higher in the observation group after treatment than in the control group (P＜0.05). There was no significant difference in the toxic side effects between the two groups (P＞0.05). The survival rate in the observation group was higher than that in the control group(15.25% vs.3.39%, P＜0.05). Conclusion: Immunosuppressants combined with platinum-containing chemotherapy can inhibit the expression of tumor markers, enhance immune function, improve quality of life and improve survival in patients with advanced NSCLC.

Objective: A conductor like screening model for real solvents (COSMO) was used to predict the extraction of emodin from Polygonum multiflorum in solvents, to screen the best extraction solvent, and to explain the extraction mechanism. Methods: The COSMO model was used to predict the extraction of emodin from Polymonium multifum by natural deep eutectic solvents (NADESs) prepared with 1, 3-propylene glycol-lactic acid (1∶3) and traditional deep eutectic solvents(DESs), and the accuracy of the model prediction was verified by experiments. The extraction mechanism was further explained. Results: The COSMO model prediction, emodin and 1, 3-propanediol-lactic acid (1∶3) NADES interactions for 762.897 2 kJ/mol, significantly higher than that of emodin and interaction energy between methanol and water, the greater the interaction energy, the higher extraction efficiency, The extraction efficiency of emodin was predicted to be 1, 3-propanediol-lactic acid (1∶3)＞ methanol ＞ water. Further test validation that the extraction yield of emodin by NADES was 1.126 6 mg/g, and the extraction efficiency was 16.07 times that of water and 1.19 times that of methanol. The overall trend was consistent with the predicted results of COSMO model. Conclusion: COSMO model not only can accurately predict the dissolution rule of emodin in different solvents, also can be explained from the Angle of the micro extraction mechanism, can provide all kinds of natural products efficiently provide certain reference for the selection of extraction solvent.

Objective: To improve the stability of Brucella infection inhibitory peptides by chemical modification. Methods: Firstly, the brucella infection inhibitory peptide OP11 was synthesized by solid-phase peptide synthesis ( SPPS ), and then OP11 was oxidized to obtain cyclic OP11. After that, the cyclic OP11 was directly modified by ‘one-pot method’ with two branches and four branches, respectively, and the results were verified by HPLC and NMR experiments. Results: Two-branched and four-branched cyclic OP11 peptides were successfully synthesized. Conclusion: The “one-pot method” can effectively solve the problem of synthesis of dendritic peptides by Click reaction of insoluble peptides containing disulfide bonds with poor water solubility.