Objective: To analyze the trend of stroke incidence in Chinese and Indian residents from 1990 to 2019, and to predict the change of stroke incidence in the two countries by 2042, and to explore the influence of age, period, birth cohort and other factors on the incidence of stroke in the two countries, so as to provide scientific reference for control measures. Methods: Based on the data of the Global Burden of Disease 2019 (GBD2019), the Joinpoint regression model was used to analyze the incidence trend, the age-period-cohort (APC) model was used to study the influence of different factors, and the Bayesian model was used to predict the future trend. Results: From 1990 to 2019, the overall incidence of stroke in China and India showed a downward trend. The Joinpoint regression model showed that the incidence rate decreased by 0.35% per year on average in China (AAPC=-0.35%, P＜0.001) and 0.32% per year in India (AAPC=-0.32%, P＜0.001). The APC model showed that the risk of stroke increased with age and period, and decreased with birth cohort. It was expected that by 2042, the incidence of stroke in China would continue to decline, while India would show an upward trend. Conclusion: The risk of stroke is significantly affected by population aging. Although the overall trend is declining, stroke is still an important public health problem in both countries.

Objective: To explore the mental health status and influencing factors of medical staff in oncology department of east areas of Shandong province, and to provide basis for psychological assessment and intervention. Methods: From September to December 2023, a total of 720 medical staff in the oncology department of a tertiary hospital in east areas of Shandong province were selected. The symptom self-rating scale was used to investigate the influencing factors, and multiple linear regression analysis was used to analyze the influencing factors. Results: The mental health scores of depression, anxiety, hostility and terror of doctors were lower than those of nurses. After occupational stratification, the mental health scores of female, master′s degree or above, married, working years ＞10 years, family monthly income ＜10 000 yuan and weekly night shift frequency ＞3 times were higher in the doctor group (P＜0.05), while in the nurses group, only women and those with family monthly income ＜10 000 yuan had a higher mental health score (P＜0.05). Multiple linear regression showed that gender was an important influencing factor, and education level only had an important impact on doctors. Conclusion: There are significant differences in the mental health status of medical staffs in oncology department of eastern areas of Shandong province. It is necessary to strengthen the mental health education of women and low-income people, give support and guidance, and improve their mental health level.

Objective: To improve the quality standard of Lidorei-7 decoction. Methods: The qualitative identification of Toosendan Fructus and gardenia in Lidorei-7 decoction was carried out by thin Layer chromatography (TLC). The content of geniposide in the formula was determined by high performance liquid chromatography (HPLC). The chromatographic conditions were as follows: amino-bonded silica gel column, according to the method of Chinese Pharmacopoeia (2020 edition), the column temperature was set at 30 ℃, the mobile phase acetonitrile-water system was used for gradient elution, the detection wavelength was set at 238 nm, and the flow rate was 1.00 mL/min. The examination item of Mongolian medicine preparation Lidorei-7 decoction was studied. Results: The characteristics of the identification of gardenoside in Mongolian medicine Lidorei-7-decoction were obvious by thin Layer chromatography, and the negative control had no interference and demonstrating strong specificity. The HPLC method was used to determine the total content of gardenoside, the results were rapid, accurate, stable, repeatable, excellent linear results and good average recovery. Conclusion: The identification, inspection and content determination methods established in this experiment are scientifically feasible, sensitive, reproducible and accurate, which provide a precise scientific basis for establishing the quality standard of Lidori-7 decoction, and can be used for the quality control of Mongolian medicines such as Lidori-7 decoction.

Objective: To study the effects of different concentrations of D-ribose on the proliferative viability, intracellular ATP level and mitochondrial respiratory chain-related gene expression of K562 cells. Methods: K562 cells were incubated with different concentrations of D-ribose and D-glucose in basal medium or PBS for 6, 24 or 48 hours, and the proliferation viability, intracellular ATP level and the expression of genes related to mitochondrial respiratory chain (MTND6, MTATP8) were detected by using CCK-8, ATP detection kit and real-time fluorescence quantitative PCR (RT-qPCR), respectively. Results: Unlike the proliferative effect induced by D-glucose, low concentrations of D-ribose (10-25 mmol/L) had no significant effect on cell proliferation within 24 hours, but significantly inhibited cell proliferation after 48 hours. The results of ATP detection showed that the ATP level was generally increased in the basal medium for 24 h, which was different from that in the glucose group, and only the ATP level was increased in the 50 mmol/L D-ribose group; after 48 h, although the ATP in the control group was almost depleted, the ATP in the 10 mmol/L and 20 mmol/L ribose groups was significantly higher than that in the control group. Under the condition of sugar-free (PBS), unlike D-glucose, which significantly increased ATP levels, D-ribose could not effectively maintain ATP levels. RT-QPCR showed that D-ribose significantly inhibited the expression of mitochondrial respiratory chain gene MTATP8 (P＜0.01), but had no effect on the expression of MTND6. Conclusion: High concentration of D-ribose can inhibit the proliferation of K562 cells and the expression of respiratory chain genes, and has little contribution to the promotion of ATP production, but may reduce the utilization of ATP. This study lays an experimental foundation for further understanding the function of D-ribose in maintaining metabolic homeostasis.

Objective: To investigate the regulatory role of miR-17-5p in the osteogenic differentiation of suture mesenchymal stem cells (SuSCs) and further explore its potential mechanism. Methods: (1)SuSCs of the cranial suture in SD rats were extracted and cultured. After induction with osteogenic induction solution, the expression levels of osteogenic markers (ALP, OCN, Runx2, BMP2) were detected by alkaline phosphatase (ALP) staining, alizarin red staining and RT-qPCR, respectively. The osteogenic ability was identified, and the purity of the extracted SuSCs was identified by flow cytometry. (2)The overexpression and interference vectors of miR-17-5p were constructed and transfected into SuSCs cells. The expression levels of osteogenic markers (ALP, OCN, Runx2, BMP2) were detected by alkaline phosphatase (ALP) staining, alizarin red staining and RT-qPCR, respectively. The effects of overexpression or interference of miR-17-5p on osteogenic differentiation of SuSCs were detected. (3)After overexpressing or interfering with miR-17-5p and transfecting SuSCs cells, WB was used to detect the mechanism of its effect on TGF-β and Smad7. Results: SuSCs cells were successfully extracted. The results of ALP staining, alizarin red staining and RT-qPCR detection of osteogenesis-related markers (ALP, OCN, Runx2, BMP2) after overexpression of miR-17-5p in SuSCs showed a trend of promoting osteogenic differentiation. After SuSCs interfered with miR-17-5p, ALP staining, alizarin red staining and RT-qPCR detection of osteogenic markers (ALP, OCN, Runx2, BMP2) showed a trend of inhibiting osteogenic differentiation. WB results showed that compared with the blank control group, overexpression of miR-17-5p significantly increased the expression level of TGF-β protein and significantly decreased the expression level of Smad7 protein, while interfering with miR-17-5p showed the opposite results. Conclusion: miR-17-5p is involved in the osteogenic differentiation of SuSCs and exerts a critical regulatory role by modulating the TGF-β/Smad7 signaling pathway.

Objective: To construct a prokaryotic expression vector for the hemolysin co-regulated protein (Hcp) gene from Acinetobacter baumannii strain 5075, and to achieve high-efficiency expression and purification of the protein. Methods: Based on the bioinformatics analysis of Hcp protein, the target gene was amplified by PCR. The target fragment and pET-28a(+) plasmid were digested with BamHⅠ and XhoⅠ, ligated and transformed into E.coli DH5α, and screened on LB solid medium containing kanamycin. Single colonies were randomly selected for colony PCR identification and sequencing. The recombinant plasmid pET-28a(+)-Hcp was transformed into E.coli BL21 (DE3) again to explore the suitable induction conditions. The expression of Hcp protein was induced by IPTG at 37 ℃ and 18 ℃, respectively, and the target protein was purified by BeyoMag^TM His tag protein purification agarose magnetic beads (NTA-Ni). Results: The bioinformatics analysis of Hcp protein showed that the Ab5075_Hcp gene from A. baumannii encodes 167 amino acids, with a protein formula of C₈₃₃H₁₂₇₈N₂₂₈O₂₆₀S₃, the theoretical protein molecular weight was 18.7 kDa, the protein isoelectric point was 6.52, the instability index was 32.86, the aliphatic index was 67.13, and the average hydrophilicity was -0.625, which was a hydrophilic stable protein. There was no transmembrane region and signal peptide, and the sequence is highly conserved. The secondary structure mainly had regular curl and extension chain. Hcp protein was located in the nucleus and had five potential B cell epitopes. In this study, the prokaryotic expression vector of Hcp gene was successfully constructed, and a large amount of soluble Hcp protein was purified after induction expression. The optimal induction conditions were IPTG 0.5 mmol/L and induction at 18 ℃ for 15-16 h. Conclusion: The Ab5075_Hcp protein of Acinetobacter baumannii is within a reasonable range of predicted values and has a good purification effect, which is suitable for the preparation of antibodies and candidate vaccines.

Objective: In this study, the gastric injury model of rats induced by anhydrous ethanol was used to verify the gastric protective effect of special dietary Hewei peptide, and the related pathway targets were screened by network pharmacology, which provided experimental basis for clinical application. Methods: Male SD rats were selected for preventive administration, and different doses of Hewei peptide and positive drug ranitidine were given respectively. After the administration, except for the normal control group, anhydrous ethanol was used to establish the model for 1 h. Abdominal aortic blood was collected and gastric tissue was taken. The morphological characteristics of gastric wall were observed by naked eye and HE and PAS staining, and the expression of interleukin-6 ( IL-6 ) and catalase ( CAT ) in serum was detected. The chemical constituents of each drug in Hewei peptide were obtained from TCMSP and HERB databases, and Venn diagram was drawn by searching for relevant targets in GeneCards and OMIM databases. PPI network diagram was drawn by STRING website, Cytoscape3.9.1 software and DAVID platform, and GO and KEGG enrichment analysis was carried out. CytoScape 3.9.1 software was used to construct the drug-active component-disease-target network diagram of Hewei peptide, and to predict the core targets and main active components of Hewei peptide for gastric protection. Results: The results of physiological and biochemical indexes and HE and PAS staining in animal experiments showed that special diet and Weitai peptide had protective effects on acute gastric injury induced by anhydrous ethanol in rats. The results of network pharmacology showed that Hewei peptide may play a role in preventing gastric injury by acting on core targets such as TP53, EGFR and AKT1, and regulating inflammatory signals such as PI3K-Akt and MAPK. Conclusion: Hewei peptide plays a role in preventing gastric injury by regulating the level of inflammatory factors, reducing oxidative stress, maintaining the integrity of gastric mucus barrier, and regulating PI3K-Akt, MAPK and other inflammatory signaling pathways.

Objective: To investigate the effect of miR-23a-3p on the activation of human hepatic stellate cells (LX-2) and to explore its related mechanisms. Methods: The expression levels of miR-23a-3p and PTEN genes and proteins such as α-SMA and collagen I were detected by real-time quantitative polymerase chain reaction (qRT-PCR) assay and immunoprotein blotting (Western Blot); LX-2 cell lines overexpressing miR-23a-3p and PTEN were constructed by transfection; and the CCK-8 assay to detect the effect of miR-23a-3p on the proliferation of LX-2 cells; screening of possible targets of miR-23a-3p by multiple databases; and detection of the binding of miR-23a-3p and the target targets by dual luciferase reporter gene assay. Results: Overexpression of miR-23a-3p significantly promoted LX-2 cell proliferation and expression of α-SMA and collagen I proteins compared with normal LX-2 cells. miR-23a-3p could target PTEN and inhibit its expression. Overexpression of PTEN reversed the effects of miR-23a-3p on LX-2 cell proliferation and α-SMA and collagen I protein expression. miR-23a-3p promoted TGF-β1-induced phosphorylation of PI3K, Akt, and mTOR, while overexpression of PTEN inhibited miR-23a-3p-induced phosphorylation. Conclusion: miR-23a-3p promotes hepatic stellate cell activation by regulating PTEN, thereby modulating liver fibrosis.

Objective: To investigate the expression levels and clinical significance of circRNA-GRHPR in carotid atherosclerosis (CAS). Methods: A total of 124 patients were randomly selected from the Third Department of Neurology, the First Affiliated Hospital of Baotou Medical College. These patients were divided into the control group and the case group based on whether they had atherosclerosis (AS) in the carotid artery as shown by imaging examinations. In the control group, 30 patients had no plaques found in the carotid arteries; in the case group, 94 patients had plaques in the carotid arteries, which were either hard, soft, or mixed. Real-time quantitative PCR (qRT-PCR) was used to verify differential expression of circRNA-GRHPR in cells and to further analyze the role of circRNA-GRHPR in CAS. Results: The occurrence of CAS was associated with gender, age, systolic blood pressure, smoking history, alcohol consumption history, hypertension history, and peripheral blood circRNA-GRHPR expression levels, all of which were positively correlated (P＜0.05); HDL-C was associated with CAS but negatively correlated (P＜0.05). The peripheral blood circRNA-GRHPR expression level in the case group was higher than that in the control group (P＜0.05). Conclusion: The occurrence of CAS is significantly related to the expression levels of circRNA-GRHPR in patients′ serum, potentially serving as an improved diagnostic tool and a new therapeutic target for AS, paving the way for early intervention measures.

Objective: To analyze the relationship between extramembranous invasion (ENE) status, pathological features and prognosis in rectal cancer patients with lymph node metastasis. Methods: A total of 100 patients with lymph node metastasis rectal cancer who underwent radical resection in our hospital from January 2020 to June 2024 were selected. The medical records of the patients were sorted out to analyze the relationship between ENE and pathological features and prognosis. Results: Through microscopic observation, 48 patients were ENE positive and 52 patients were ENE negative in 100 patients. The ENE positive tumor budding level, nerve invasion, T stage and N stage were higher than those in the ENE negative group (P＜0.05). After 30 months of follow-up, 33 of the 100 patients relapsed, and the disease-free survival rate of the ENE positive group was lower than that of the ENE negative group (P＜0.05). Conclusion: Patients with lymph node metastasis and positive extramural invasion of rectal cancer are more aggressive, which is an important factor affecting the prognosis of patients.

Objective: To investigate the clinical significance of serum uric acid (UA) and homocysteine (Hcy) levels in patients with Parkinson′s disease (PD) complicated with cognitive impairment. Methods: A total of 96 patients with PD who were admitted from January 2017 to January 2022 were selected and divided into three groups according to the Montreal Cognitive Assessment Scale score. Group A was the PD cognitive normal group (34 patients), group B was the PD mild cognitive impairment group (32 patients), and group C was the PD dementia group (30 patients). Group D was the control group, which was composed of healthy subjects (50 patients). The levels of UA and Hcy in each group were detected, and the significance of the differences in each index was analyzed. Results: Compared with group D, the level of UA was lower and the level of Hcy level were higher in groups A, B and C, and the differences were statistically significant (P＜0.01). Compared with group A, the level of UA was lower and the level of Hcy was higher in group B and group C, the differences were statistically significant (P＜0.05). The level of UA in group C was lower than that in group B, and the level of Hcy was higher than that in group B (P＜0.05). Conclusion: The decrease of UA and the increase of Hcy level in PD patients may reflect the degree of cognitive impairment in PD patients to a certain extent.

Objective: To observe the effect and the incidence of adverse events of drug treatment and interventional treatment of aortic arch Myla classification for patients with ischemic cerebrovascular disease. Methods: A total of 120 patients with ischemic cerebrovascular disease in Bayannur City Hospital from 2018 to 2021 were selected as the research objects. According to different treatment methods, they were divided into drug group and surgical group. The drug group was treated with traditional drugs; on the basis of the classification of aortic arch, the patients in the surgical group were treated with corresponding surgical methods according to different aortic arches. The degree of vascular stenosis, operation time and incidence of adverse events were observed in the two groups. Results: (1)One vessel stenosis accounted for 55.0% in the drug group and 53.3% in the surgical group. Patients in both groups were mainly with one vessel stenosis, and there was no significant difference in the number of vascular stenosis between the two groups; (2)The surgical time of patients with more than two vascular lesions was longer than that of patients with only one vascular lesion; (3)In each type of aortic classification, the conventional technique (surgical 1) was the main surgical (79.67%), and the exchange technique (surgical 3) was the least used (5.09%). (4)Long-term follow-up results showed that the incidence of adverse events in 3 or more vascular stenosis was higher; the results of inter-group comparison showed that the incidence of adverse events in the drug group was 26.7% at 3 months after surgical, which was higher than 8.3% in the surgical group, while at 12 months after surgical, the incidence of adverse events in the drug group was 58.3% in the drug group, which was higher than 23.3% in the surgical group. Conclusion: (1) The more the number of vascular stenosis, the longer the surgical time; (2) Aortic classification is closely related to the type of operation adopted by the patients. No matter which type, the patients are mainly treated with conventional technique (surgical 1). (3)No matter what kind of treatment, the main adverse event of ischemic cerebrovascular disease is transient ischemic attack (TIA).

Objective: Analysis of clinical features and complication risk factors after impacted upper GI foreign bodies in 230 geriatric patients in Baotou area. Methods: The clinical and endoscopic data of elderly patients who underwent foreign body removal from upper gastrointestinal in the endoscopy center of the Second Affiliated Hospital of Baotou Medical College from January 2020 to December 2024 were collected and retrospectively analyzed. The χ² test was used to compare the count data of the clinical characteristics of foreign body incarceration in upper gastrointestinal. At the same time, univariate and multivariate logistic regression were used to analyze the risk factors of complications of foreign body incarceration in upper gastrointestinal. Results: A total of 230 elderly patients were included according to the criteria, including 98 males and 132 females. The age was mainly distributed between 60 and 74 years old (155 cases, 67.4%). The main type of foreign body incarceration was jujube pit (116 cases, 50.4%). The location of foreign body retention was mainly concentrated at the esophageal entrance (143 cases, 62.2%). The foreign body traits were mainly sharp foreign bodies (138 cases, 60%), and the patients with incarceration time ≤24 hours accounted for the majority (126 cases, 54.8%). There were 83 patients (36.1%) with upper gastrointestinal diseases, 98 patients (42.6%) with complications. Multivariate Logistic regression analysis demonstrated that impacted duration (＞24 hours), foreign body characteristics (sharp objects), and pre-existing upper gastrointestinal diseases were risk factors for complications secondary to impacted upper GI foreign bodies (P＜0.01). Conclusion: The complications arising from impacted upper gastrointestinal foreign bodies in elderly patients are significantly associated with the duration of impaction, physical characteristics of the foreign body, and the presence of comorbid upper GI pathologies. Clinically, relevant risk factors should be avoided, and endoscopic intervention should be performed as early as possible to effectively prevent or reduce the occurrence of complications.