Objective: To investigate the effect of seabuckthorn fruit on the proliferation and migration of lung cancer A549 cells by network pharmacology and in vitro experiments, and to explore the related mechanism. Methods: The effective components and drug targets of seabuckthorn fruit were screened by searching TCMSP database, GeneCard database and OMIM database to obtain lung cancer targets. The intersection targets of drugs and diseases were obtained by using the micro-bioinformatics platform, and then imported into the STRING website to construct the PPI network. The core target network diagram and drug-disease-target network were constructed by Cytoscape 3.10.3 software. GO and KEGG enrichment analysis was performed on the intersection targets using the Metascape database. Molecular docking visualization was performed using Discovery Studio. CCK-8 and cell scratch experiments were used to evaluate the inhibitory effect of seabuckthorn-containing serum on the proliferation and migration of lung cancer A549 cells. Results: The results of network pharmacology analysis showed that a total of 8 effective active ingredients were screened out, corresponding to 85 key targets for the prevention and treatment of lung cancer. Among them, molecular docking experiments confirmed that MMP9, CDK4, MMP2, EGFR, HIF1A and CYP1B1 targets closely related to tumor cell proliferation and metastasis had good binding activity with quercetin, isorhamnetin, kaempferol and stigmasterol. In vitro experiments showed that 10% seabuckthorn fruit-containing serum could inhibit the proliferation of A549 cells and reduce the wound healing rate after 24 h. Conclusion: Seabuckthorn fruit has a potential preventive and therapeutic effect on lung cancer, which can inhibit the proliferation and migration of A549 cells.

Objective: To explore the mechanism of Caowu-Hezi in the treatment of rheumatoid arthritis (RA) by network pharmacology and molecular docking technology. Methods: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and literature were used to screen the effective components of Caowu-Hezi. The potential targets of the effective components were screened by Swiss Target Prediction and other databases. Based on authoritative databases such as OMIM, RA-related potential target data sets were screened. Venny online tool was used for target intersection analysis, and the screening results were imported into Cytoscape 3.7.2 software to construct protein-protein interaction networks (PPI). Gene ontology (GO) functional annotation and Kyoto encyclopedia of genes and genomics (KEGG) pathway enrichment analysis of key targets were performed through the online analysis platform. Finally, Autodock Tools molecular docking software was used to verify the molecular docking of key target proteins and potential active compounds. Results: A total of 16 active components were identified and 109 potential targets related to RA were screened out. Among them, the key targets were signal transduction and transcriptional activator protein 3 (1,STAT3), Toll-like receptor 4 (2,TLR4), transcription factor JUN, interleukin-6 (3,IL-6) and serine protein kinase (4,AKT1). Bioinformatics analysis showed that Toll-like receptor signaling pathway, tumor necrosis factor (5,TNF) signaling pathway and PI3K-Akt signaling pathway might be involved in the regulation of the pathological process of RA. Molecular docking experiments confirmed that the active components of Caowu-Hezi had high binding affinity with the above core targets (such as STAT3, TLR4, JUN, etc.), suggesting its potential regulatory role. Conclusion: Caowu-Hezi may affect the activity of target proteins such as AKT1, JUN, IL-6, STAT3 and TLR4 by regulating key signaling pathways such as Toll-like receptor, TNF and PI3K-Akt, thus exerting anti-RA effect.

Objective: To express Echinococcus multilocularis ferritin (EmFer) in prokaryotes and to bioinformatically analyze this protein T- and B-cell epitopes, and to prepare the corresponding polyclonal antibodies. Methods: The subcellular localization and secondary and tertiary structures of EmFer were analyzed using online software, and its T and B cell epitopes were predicted. The Emfer gene was amplified by PCR using cDNA from E. multilocularis protoscoleces as a template, and then cloned into the pET28a(+) vector to construct the recombinant plasmid pET28a(+)-EmFer. After PCR, restriction enzymes identification and sequencing, the recombinant plasmid was transformed into BL21(DE3) and expressed by isopropyl-β-d-thiogalactoside (IPTG) induction. The expression of recombinant EmFer (rEmFer) was analyzed and identified by 12.5% SDS-PAGE electrophoresis and Western Blot. Polyclonal antibodies were prepared by immunizing mice with purified rEmFer, and the antibody titer and its reactivity with recombinant protein were detected by ELISA and Western Blot. Results: Subcellular localization prediction indicated that the protein might be located in the cytoplasm, and T and B cell epitope prediction showed that EmFer had 6 T and B cell combined epitopes, namely 5 aa-10 aa, 38 aa-40 aa, 57 aa-61 aa, 118 aa-120 aa, 122 aa, and 139 aa. The recombinant plasmid pET28a(+)-EmFer was successfully constructed and transformed into BL21(DE3) competent cells. After induction, expression and purification, the insoluble recombinant protein rEmFer with high purity was obtained, and the relative molecular mass was about 22 000 (including the expression vector sequence). The titer of the polyclonal antibody obtained after immunization of mice was 1∶1 280 000, and it could specifically bind to rEmFer. Conclusion: The rEmFer is successfully expressed and exhibits favorable immunogenicity. It is predicted to contain T-cell and B-cell epitopes. These findings provide a theoretical basis for the prevention and control of echinococcosis and for vaccine development.

Objective: To investigate whether CXCL3 is involved in cerebral ischemia-reperfusion injury (CIRI) by mediating inflammatory response. Methods: The SD male rats were randomly divided into three groups: the sham operation group, the model group, and the CXCL3 injection group (200 ng per rat). The sham surgery group only underwent surgical exposure of the blood vessels. The model group established a middle cerebral artery occlusion/reperfusion (MCAO/R) model using the Longa suture method, with 1 hour of ischemia followed by 24 hours of reperfusion. The CXCL3 injection group used stereotactic brain surgery to inject recombinant CXC chemokine ligand 3 (CXCL3) protein into the lateral ventricle on the side of the MCAO/R model. The local cerebral blood flow changes in the MCAO model were detected using the moorFLPI-2 laser speckle perfusion imager. The degree of neurological dysfunction in rats was assessed using a neurological function scoring system. The volume of cerebral infarction was measured by TTC staining. The protein expression levels of CXCL3, interleukin-1 (IL-1) and interleukin-6 (IL-6), were measured by western blotting (WB). Results: Compared with the baseline blood flow, the blood flow in the MCAO model rats decreased by approximately 70% (P＜0.001). Compared with the blood flow after 1 hour of ischemia, the blood flow values significantly increased after suture was removed (P＜0.001). Additionally, there was no significant difference compared with the baseline blood flow (P＞0.05). Twenty-four hours after reperfusion, compared with the sham operation group, the neurological function score and cerebral infarction volume in the model group both significantly increased (P＜0.001). Compared with the sham operation group, the expression of CXCL3 protein in the ischemic area of the model group was upregulated (P＜0.001), and the expressions of the inflammatory factors IL-1 and IL-6 were both increased (P＜0.05). Compared with the model group, injection of recombinant CXCL3 protein further upregulate the expression of CXCL3 protein (P＜0.05), and significantly increased the expression levels of the inflammatory factors IL-1 and IL-6 (P＜0.05). Conclusion: High level of CXCL3 may contribute to CIRI by upregulating the expression of pro-inflammatory factors.

Objective: The complex pathogenesis of myocardial ischemia reperfusion injury (MIRI) has become the main reason affecting the prognosis of patients. MicroRNAs (miRNAs) and pyroptosis are closely related to the pathological mechanism of MIRI, but the specific mechanism is not clear. The purpose of this study was to investigate whether miR-193b-3p can protect damaged myocardium by inhibiting pyroptosis and provide a potential treatment for MIRI. Methods: Forty-eight male SD rats were randomly divided into sham operation group, MIRI group, miR-193b-3p negative control group and miR-193b-3p overexpression group. The rats in MIRI group were subjected to ligation of the left anterior descending coronary artery for 30 min and reperfusion for 72 h. The rats in miR-193b-3p negative control group and miR-193b-3p overexpression group were given myocardial injection of corresponding reagents at the beginning of reperfusion. The rats in sham operation group were only threaded without ligation. The expression of miR-193b-3p was detected by RT-qPCR. The pathological changes of myocardium were evaluated by HE staining. The expression levels of NOD-like receptor protein3 (NLRP3) and apoptosis-associated speck-like protein containing CARD (ASC) were detected by Western blot. Combined with the expression analysis of cardiac troponin T (cTnT), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), the protective effect of miR-193b-3p in MIRI was comprehensively judged. Results: Compared with the sham operation group, the levels of cTnT, IL-1β and TNF-α in serum of rats in the MIRI group were increased (P＜0.05), and the pathological changes of myocardial tissue were significant. Compared with MIRI group and miR-193b-3p negative control group, the expression of cTnT, IL-1β, TNF-α, ASC and NLRP3 in miR-193b-3p overexpression group was decreased (P＜0.05), and the myocardial pathological changes after myocardial infarction were improved, indicating that up-regulation of miR-193b-3p expression could inhibit pyroptosis and reduce MIRI. Conclusion: In this study, direct evidence of miR-193b-3p improving myocardial injury is obtained by observing the expression of inflammatory markers and pyroptosis proteins. MiR-193b-3p can be used as a biomarker to provide a new potential target for the treatment of MIRI.

Objective: Accurately predicting phage viral proteins (PVPs) not only provides insights into the interactions between phages and their hosts, but also contributes to the development of novel antibacterial drugs. Methods: Three characteristic parameters were extracted: amino acid composition (AAC), dipeptide deviation from expected mean (DDE) and protein similarity measure (PSM). A hybrid sampling method was employed to balance the dataset, and dimensionality reduction was performed on high-dimensional features through variance analysis. The support vector machine algorithm was utilized to predict bacteriophage viral proteins (PVPs) under Jackknife testing. Results: For phage viral proteins (PVPs), after balancing the dataset using the SMOTETomek method, the highest prediction accuracy achieved with a single feature parameter was 96.41%. After dimensionality reduction, the prediction accuracy increased to 97.07%. Finally, the feature parameters were fused, resulting in the highest prediction accuracy of 97.29%. Conclusion: Employing a mixed sampling approach for unbalanced datasets, utilizing the analysis of variance method for dimensionality reduction, and appropriately integrating feature parameters can all effectively enhance the success rate of predictions.

Objective: To explore the role and molecular mechanism of down-regulation of thioredoxin system in cigarette smoke extract (CSE) -induced human bronchial epithelial cell (BEAS-2B) injury model, so as to provide experimental basis for finding potential prevention and treatment targets of smoking-related diseases. Methods: CCK-8 method was used to detect the effect of different concentrations of CSE on the viability of BEAS-2B cells, and the treatment concentration of CSE was screened to establish a cell injury model. Lentivirus LentiCRISPRv 2 plasmid was constructed to knock down thioredoxin-1 (Trx-1) in BEAS-2B cells. The cells were divided into control group, 10% CSE treatment group, knockdown group and knockdown + 10% CSE treatment group. CCK-8 method was used to detect cell activity. RT-qPCR method was used to detect the expression levels of inflammatory factors, Trx-1 and thioredoxin reductase (TrxR) mRNA. Western blot method was used to detect the expression levels of Trx-1 and TrxR proteins. Flow cytometry was used to detect the level of reactive oxygen species (ROS). Results: After CSE intervention in BEAS-2B cells for 24 hours, the cell viability of BEAS-2B cells gradually decreased with increasing CSE concentration (P＜0.05). The protein expression levels of Trx1 and TrxR were downregulated (P＜0.001), and their mRNA expression levels were also downregulated (P＜0.05). The mRNA expression levels of the inflammatory cytokines IL-6, IL-8, and IL-1β in the cell model were upregulated (P＜0.001), and the ROS level was increased (P＜0.000 1). Following Trx-1 knockdown, compared with the control group, cell viability after CSE exposure was more markedly decreased (P＜0.000 1), and the mRNA expression levels of the inflammatory cytokines were all increased (P＜0.000 1). Conclusion: CSE induces injury in BEAS-2B cells. Decreased Trx-1 levels in the cells exacerbate CSE-induced inflammatory responses and oxidative stress injury in BEAS-2B cells. These findings suggest that downregulation of the Trx system may mediate CSE-induced injury in BEAS-2B cells.

Objective: To explore the effects of endothelin-1 (ET-1) on the calcium transient frequency and electrophysiological signals of human heart organoids (hHOs) induced by human induced pluripotent stem cells (hiPSC). Methods: The construction and verification of hHOs were carried out, and hiPSC was differentiated into hHOs, which was verified by morphology and immunofluorescence analysis. The effects of ET-1 on calcium transient frequency and electrophysiology of hHOs were studied. Except for the control group, the other groups were treated with corresponding concentrations of ET-1 for 0.25 h, 1 h, 4 h and 48 h, respectively. After treatment, Ca²⁺ imaging and electrophysiological analysis of hHOs were detected. Results: The constructed hHOs achieved a single chamber and uniform beating. The hHOs cultured for 30 days showed positive expression of various typical cardiac cell types and tissue structures, including cTnT, epicardial marker WT1, ventricular myocytes MYL2 and atrial myocytes MYL7. ET-1 (100 nM) had no significant effect on hHOs (0-1 h), while 1 μM ET-1 treatment increased the frequency of Ca²⁺ transients at the same time. Both 100 nM and 1 μM ET-1 treatment (0-8 h) reduced the transient frequency of Ca²⁺ in hHOs, and there was no significant change in the blank control group within 8 h of dimethyl sulfoxide (DMSO) exposure. The electrophysiological results showed that 100 nM and 1 μM ET-1 (1-48 h ) treatment led to a decrease in pulse frequency, and 1 μM ET-1 (0-1 h) treatment increased the field potential frequency, the difference was statistically significant (P＜0.01). Conclusion: ET-1 can affect the calcium transient frequency and electrophysiological signal generation of hHOs in a concentration-and time-dependent manner by regulating Ca²⁺ channels and affecting the contractile function of cardiomyocytes, suggesting that it may have cardiotoxicity and cause pathological remodeling risk.

Objective: To investigate the expression of Schlafen family member 11 (SLFN11) in breast cancer and its correlation with the expression of immune escape related molecules. Methods: TCGA and TIMER 2.0 databases were used to predict the expression of SLFN11 in tumors. TIMER 2.0 was used to predict the relationship between SLFN11 and immune cell infiltration. The effects of SLFN11 on the expression of immune cells, immunosuppressive agents and immunopotentiators in breast cancer were analyzed by TISIDB database. The expression of SLFN11 mRNA in breast cancer cell lines was detected by qRT-PCR. Results: The expression of SLFN11 was down-regulated in breast cancer, which was associated with TP53 mutation and SLFN11 promoter methylation. TIMER 2.0 database predicted that the expression of SLFN11 in breast cancer was related to the infiltration of B cells, CD8+ cells and CD4+ cells. The TISIDB database predicted that the expression of SLFN11 was positively correlated with lymphocytes such as TH1, NKT and TfH (P＜0.05), positively correlated with immunosuppressive molecules such as PDCD1LG2, CD96 and TIGT (P＜0.05), and positively correlated with the expression of major histocompatibility complex (MHC) molecules such as LA-DRA, HLA-DMB and HLA-DOA (P＜0.05). Quantitative analysis showed that compared with normal breast cells, the expression of SLFN11 in breast cancer cells was down-regulated (P＜0.01), and SLFN11 in paclitaxel-resistant cells was lower than that in corresponding breast cancer parent cells (P＜0.05). Conclusion: The expression of SLFN11 is down-regulated in invasive breast cancer, which is related to the number of lymphocytes, immune cell infiltration and the expression of immunosuppressive molecules.

Objective: To explore the effect of over-expressing WNT11 on osteogenesis of osteoblasts under LPS-incurred inflammatory condition in vitro. Methods: The objective gene sequences targeting for WNT11 were transduced into osteoblasts by lentivirus, then the WNT11 protein level was tested by Western blot assay from the functional perspective. The cells after transduction were evaluated the cell proliferation activity by the CCK-8 method. The experimental cells were enrolled into 3 groups in vitro: non-induced group with LPS, general induced group with LPS and over-expressing WNT11 induced group with LPS. All cells were induced in vitro by alizarin red staining, mineral nodules and alkaline phosphatase (ALP) semi-quantitative analysis, and qRT-PCR was used to analyze the expression levels of osteogenic specific genes ALP, runt-related transcription factor 2 (Runx2), osteocalcin (OC) and collagen I mRNA after induction, and the differences in osteogenic ability were comprehensively evaluated. Results: After transduction using lentivirus, the results of Western blot assay showed the WNT11 protein level was significantly higher than that in control group (P＜0.001). The cell proliferation activity demonstrated no significant differences after transduction, when compared with non-transduction at any time point (P＞0.05). There was no significant apoptosis of osteoblasts cultured in LPS (100 ng/mL) concentration. After 1 week and 2 weeks of induction, mineralized nodules were observed in the induction group and gradually increased with time, while no obvious mineralized nodules were observed in the control group.The quantitatively analysis results of mineralization nodules and ALP activity were significant higher in WNT11 over-expressing group than in general induced group (P＜0.000 1). Moreover, the results of osteogenic relate genes demonstrated similar trends. Conclusion: Over-expressing WNT11 may significantly enhances the osteogenic ability of osteoblasts under LPS-incurred inflammatory condition.

Objective: To investigate the clinical risk factors in patients with sepsis complicated with intestinal injury. Methods: From May 2023 to March 2024,68 patients with sepsis and intestinal injury in intensive care unit (ICU) of the First Affiliated Hospital of Baotou Medical College were prospectively observed and analyzed. Results: The levels of sphingosine kinase 1 (Sphk1), sphingosine 1-phosphate (S1p), sphingosine 1-phosphate receptor 1 (S1pr1), sphingosine 1-phosphate receptor 2 (S1pr2), D-lactic acid, acute physiology and chronic health evaluation score (APACHE Ⅱ) and mortality in patients with acute gastrointestinal injury (AGI) grade Ⅲ-Ⅳ were higher than those in AGI grade Ⅰ-Ⅱ (P＜0.05). The APACHE Ⅱ score and mortality of AGI grade Ⅳ were higher than those of AGI grade Ⅲ (P＜0.05). With the increase of AGI grade, the level of intestinal fatty acid binding protein (I-FABP) increased (P＜0.05). The AGI grade, ICU stay time and APACHE Ⅱ score in the death group were higher than those in the survival group (P＜0.05). The levels of Sphk1, S1P, S1PR1, S1PR2 and lactic acid (Lac) in the death group were higher than those in the survival group (P＜0.05). Logistic analysis showed that Lac, APACHE Ⅱ score and AGI grade may be independent influencing factors of 28-day death. The area under the curve (AUC) of combined prediction of Lac, APACHE Ⅱ score and AGI grade was 0.911, the sensitivity was 94.4% and the specificity was 80.0%. Conclusion: With the increase of AGI grade, Sphk1, S1P, S1pr1, S1pr2, D-lactic acid, IFABP, APACHE Ⅱ score and mortality also increased. Lac, APACHE Ⅱ score and AGI grade may be independent risk factors for death in patients with sepsis complicated with intestinal injury, and the combination of the three has good prognostic value.

Objective: To explore the clinical diagnostic value of magnetic resonance imaging (MRI) combined with blood biochemical markers in multiple sclerosis. Methods: Thirty patients with multiple sclerosis admitted from January 2023 to September 2024 were selected as the observation group. According to the severity of the disease, they were divided into mild group (18 patients) and severe group (12 patients). At the same time, 50 healthy subjects were selected as the control group. The levels of blood biochemical markers in the observation group and the control group were compared. After MRI examination, the MRI imaging features of patients with multiple sclerosis were analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of MRI combined with biochemical markers for multiple sclerosis. Results: (1) 135 pathological changes were found in 30 patients with multiple sclerosis by MRI, and the main lesions were distributed in brainstem, periventricular, cerebellum and white matter. (2) MRI plain scan showed low signal in T1, high signal in T2 and FLAIR sequences, and the lesions around the lateral ventricle were perpendicular to the long axis. MRI coronal and sagittal plain scan T2 showed high signal, showing multiple spatial distribution. (3) The levels of serum neuron-specific enolase (NSE), myelin basic protein (MBP) and serum S100 calcium-binding protein B (S100B) in the observation group were higher than those in the control group (P＜0.05). The levels of serum NSE and S100B in the severe group were higher than those in the mild group (P＜0.05). (4) ROC curve analysis showed that the sensitivity, specificity and accuracy of MRI combined with biochemical markers for multiple sclerosis were 87.50%, 93.75% and 91.25%, respectively, which were higher than those of MRI and biochemical markers alone. Conclusion: Blood biochemical markers combined with MRI detection can not only show the lesions of multiple sclerosis, but also help to improve the diagnostic value of the disease.

Objective: To investigate the clinical value of serum small dense low-density lipoprotein cholesterol (sdLDL-C) and homocysteine (Hcy) in the diagnosis of ischemic stroke. Methods: A total of 128 patients with ischemic stroke and 128 healthy subjects (n=128) admitted to Binzhou Central Hospital from June 2021 to July 2022 were enrolled. The serum levels of sdLDL-C, Hcy, HDL-C, LDL-C and lipoprotein Lp(a) were detected by Mindray automatic biochemical analyzer BS2000M2, and the correlation between each index and the clinical characteristics of ischemic stroke was discussed. Results: There were significant differences in the prevalence rates of basic diseases such as hypertension (64.84% vs. 42.97%), hyperlipidemia (60.16% vs. 35.93%) and drinking history (61.72% vs. 38.28%) between the observation group and the control group (P＜0.05), but no statistical differences were observed in diabetes history or smoking history between the two groups (P＞0.05). The detection of biochemical indexes showed that the concentrations of serum LDL-C (3.91±1.62)mmol/L, Lp(a) (368.07±32.04)mg/L, sdLDL-C (4.32±0.59)mmol/L and Hcy (19.71±1.09)μmol/L in the observation group were significantly increased (P＜0.01), while HDL-C (1.16±0.24)mmol/L was significantly decreased (P＜0.05). Pearson correlation analysis showed that serum sdLDL-C and Hcy levels were significantly positively correlated with the severity of ischemic stroke, infarct size and hypertension (P＜0.05), while HDL-C, LDL-C and Lp(a) were not significantly correlated (P＞0.05). The serum concentrations of sdLDL-C and Hcy in severe patients were significantly higher than those in moderate and mild patients (P＜0.05), and showed an upward trend with the aggravation of the disease. Conclusion: Serum sdLDL-C and Hcy concentrations are closely related to the progression of ischemic stroke, and can be used as important diagnostic indicators for disease diagnosis and disease assessment.

Objective: To explore the value of computed tomography (CT) and magnetic resonance imaging (MRI) in the diagnosis of benign and malignant vertebral compression fractures, and to analyze their imaging features, so as to provide data support for clinical selection of reasonable imaging diagnosis methods. Methods: From October 2022 to October 2024, 75 patients with vertebral compression fractures admitted to the 990th Hospital of the Joint Logistic Support Force of the Chinese People′s Liberation Army were selected as the research objects. All patients underwent CT and MRI examinations and were diagnosed by clinical pathological examination. Taking the pathological examination results as the gold standard, the diagnostic value of CT and MRI for benign and malignant vertebral compression fractures was statistically analyzed, and the CT and MRI imaging features of vertebral compression fractures were analyzed. Results: The results of pathological examination showed that the number of benign and malignant vertebral compression fractures was 25 cases and 50 cases, respectively. The detection cases of benign vertebral compression fractures by CT and MRI were 18 cases and 23 cases, respectively. The detection cases of malignant vertebral compression fractures were 40 cases and 48 cases, respectively. The benign detection rates were 72.00% and 92.00%, and the malignant detection rates were 80.00% and 96.00%, respectively. There was no significant difference in the benign detection rate between MRI and CT (χ²=3.388, P＞0.05), while the malignant detection rate of MRI was higher than that of CT (χ²=6.061, P＜0.05), and the sensitivity, negative predictive value and accuracy of MRI were higher than those of CT (P＜0.05). The occurrence rate of normal intervertebral disc in patients with benign vertebral compression fracture (12.00%) was lower than that in patients with malignant vertebral compression fracture (80.00%), and the occurrence rate of intervertebral disc compression (64.00%) was higher than that in patients with malignant vertebral compression fracture (14.00%) (P＜0.05). ROC curve analysis showed that the area under the curve (AUC) of CT and MRI for distinguishing benign from malignant vertebral compression fractures were 0.760 and 0.940, respectively; the diagnostic value of MRI was higher, and its sensitivity and specificity were 96.00% and 92.00%, respectively. Conclusion: Compared with CT examination, MRI examination can effectively improve the detection rate of malignant vertebral compression fractures, and improve the sensitivity, negative predictive value and accuracy. Intervertebral disc space changes can be used as the main imaging features for differential diagnosis of benign and malignant vertebral fractures.

Objective: To observe the effect of foot reflexotherapy on negative emotions, sleep quality and quality of life in patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Methods: Based on the principle of randomization, 80 patients with AMI who underwent PCI in emergency internal medicine department from October 2023 to August 2024 were randomly divided into observation group and control group. The control group was treated with standard nursing program, and the observation group was treated with foot reflexotherapy in addition to standard nursing. Statistical methods were used to analyze the differences in Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), Pittsburgh Sleep Quality Index (PSQI) and quality of life between the two groups. Results: Compared with before intervention, the scores of SAS, SDS and PSQI scales in the two groups showed a decreasing trend, and the observation group showed more obvious efficacy improvement (P＜0.05). The scores of 36-item short-form items in the two groups generally increased, and the data of each index in the observation group were significantly higher than those in the control group (P＜0.05). Conclusion: For AMI patients undergoing PCI surgery, foot reflexotherapy can effectively improve their mental status, relieve their negative emotions, increase sleep time, improve the quality of life, and accelerate the rehabilitation process.

Objective: To report a case of acute myocardial infarction caused by pregnancy complicated with supraventricular tachycardia, in order to provide a reference basis for the diagnosis and treatment of this disease. Methods: A retrospective analysis was conducted on the clinical data of a patient with acute myocardial infarction caused by pregnancy complicated with supraventricular tachycardia, including medical history, examination results, diagnostic process and treatment plan. Results: The patient suffered from acute myocardial infarction due to supraventricular tachycardia. By adopting restrictive examination and cautious medication strategies, the treatment was successfully completed, and ensure the safety of mother and child. Conclusion: The risk of acute myocardial infarction during pregnancy is high. Multidisciplinary collaboration is required to balance the safety of both the mother and the baby, and formulate individualized diagnosis and treatment plans.