Objective: To clarify whether general cistanosides (GCs) ameliorates cognitive dysfunction in rapidly aging mice (SAMP8) by mediating specific androgen receptor (AR) in regulation of expression of synaptic protein. Methods: A total of 76 7-month-old male SAMP8 mice were randomly divided into the model group, GCs group, GCs + flutamide (F) group, GCs + fulvestrant (ICI) group, F group, and ICI group. The learning and memory functions of mice in each group were tested with Morris water maze. Western blotting and RT-PCR were used to test the expression level of synaptophysin (SYN), postsynaptic density-95 (PSD-95) and the brain-derived neurotrophic factor (BDNF) of mice in each group. Results: GCs significantly improved the learning and cognitive function of SAMP8 mice. Comparing with the GCs group, the learning and memory function of mice in the GCs+F group and GCs+ICI group was significantly lowered. Western blotting and RT-PCR results showed that the expression level of BDNF, SYN, and PSD-95 were significantly increased at the protein level and gene level in the GCs group compared with the Model group (P＜0.05). SYN expression level was increased in the GCs+F and GCs+ICI group, and expression levels of BDNF and PSD-95 were significantly decreased in the GCs+F and GCs+ICI group compared with the GCs group (P＜0.05). Conclusion: GCs may specifically mediate AR to enhance synaptic plasticity in SAMP8 mice, which in turn improve learning and cognitive function.

Objective: To study the potential targets and mechanisms of curcumol against glioma using network pharmacology and in vitro experiments, and to provide theoretical basis for its clinical application and scientific research. Methods: TCMSP database and Swiss database were used to screen the potential targets, geneCards database and OMIM database were used to obtain glioma-related targets. The intersection targets of curcumol-glioma were obtained and the Wayne diagram was drawn. The PPI network was constructed using STRING database and the core targets were screened. Metascape database was used for GO and KEGG enrichment analysis to further explore the pharmacological mechanism of curcumol against glioma. Finally, MTT method was used to detect the proliferation effect of curcumol on glioma U87 cells, and western blot was used to detect the expression levels of PI3K, p-AKT and AKT proteins, to further confirm the results of network pharmacology analysis. Results: A total of 4 821 glioma targets were obtained from 102 potential targets predicted by curcumol. There were 67 intersection targets between curcumol and glioma, and the main core targets were MAPK1, EGFR, MAPK8, MAPK14 and JAK3. GO enrichment analysis showed 1 003 biological processes, including 854 BFs, 72 MFs, and 77 CCs. KEGG analysis showed 136 signaling pathways, among which PI3K / AKT pathway may play a key role in anti-glioma. Molecular docking showed that zedoaryl could spontaneously combine with EGFR, JKA3, MAPK1, MAPK8 and MAPK to form a relatively stable conformation. The results showed that the inhibitory activity of curcumol on the proliferation of glioma U87 cells was significantly enhanced in a time-and concentration-dependent manner with the increase of concentration. WB results showed that curcumol could reduce the expression of p-AKT protein in glioma U87 cells to inhibit the PI3K / AKT pathway (P＜0.01). Conclusion: Curcumol could inhibit malignant glioma with multiple targets and multiple pathways, and its mechanism may be related to the regulation of p-AKT protein expression.

Objective: To screen piRNA pathway related genes associated with diagnosis and prognosis of colorectal cancer (CRC). Methods: The expression of 39 piRNA pathpathy-related genes at the tissue level was analyzed based on 647 colorectal tumor samples and 51 non-tumor samples from the Cancer Genome Atlas (TCGA) database, and the risk prediction model was constructed. KM and ROC were used to evaluate the prediction performance and effect of the measurement model. Finally, candidate molecules were selected based on clinicopathological features and validated at the cellular level. Results: Among 37 piRNA pathway related genes with significant differences, 8 genes co-constructed the risk prediction model. KM analysis showed that OS decreased significantly in high-risk group. The ROC curve showed that the area under the curve of the model was 0.624, 0.669, and 0.670 in 1, 3, and 5 years respectively. The expression levels of POLR2F and PAICS were overexpressed in colorectal cancer cells and CTPAC Database data, and correlated with clinicopathological features. In particular, the expression level of PAICS was positively correlated with tumor immune infiltrating cells in CRC, and significantly lower in cancer-associated fibroblasts. Conclusion: piRNA pathway related genes, especially POLR2F and PAICS, may be the potential biomarkers for cancer diagnosis and prognosis in CRC.

Objective: To investigate the protective effect and possible mechanisms of 1, 25-dihydroxy vitamin D₃ on kidney in rats with type 2 diabetes mellitus. Methods: Sprague-Dawley male rats(n=40)were used in this experiments. All rats were randomly divided into the control group(n=8),model group(n=8), low-dose vitamin D₃ group(n=8),high-dose vitamin D₃ group(n=8), acarbose treatment group(n=8). Intragastric administration were continuously performed on all rats for 6 weeks and then were killed. The serum creatinine (Scr) and urea nitrogen (BUN) levels of rats were measured. The levels of superoxide dismutase (SOD), catalase (CAT), total nitric oxide synthase (tNOS) and hydrogen peroxide (H₂O₂) of renal tissues were detected. The expression of PI3K/Akt pathway related proteins in renal tissues was detected using Western blot. Results: Compared with the normal group, the contents of serum Scr and BUN in model group were significantly increased (P＜0.05). The contents of serum Scr and BUN of rats in groups with different doses of vitamin D₃ decreased significantly comparing with the model group (P＜0.05). The contents of H₂O₂ and tNOS in renal tissue in the model group increased significantly, while the levels of SOD and CAT decreased significantly comparing with the normal group (P＜0.05). Compared with the model group, all the vitamin D₃ groups could inhibit the production of H₂O₂ and tNOS and significantly increase the content of SOD and CAT in renal tissue (P＜0.05). Compared with the normal group, thep-PI3K/PI3K and p-Akt/Akt ration in the model group increased significantly (P＜0.05), but decreased significantly in vitamin D₃ groups (P＜0.05). Conclusion: 1, 25-dihydroxy vitamin D₃ has protective effect on kidney injury in type 2 diabetes rats, and the mechanism may be related to the regulation of oxidative stress through mediating PI3K / Akt signaling pathway.

Objective: To study the predictive effect of serum angiopoietin-2 on the condition of patients with community-acquired pneumonia, aiming to provide a scientific basis for early clinical intervention. Methods: From January 2020 to December 2021, 145 patients (81 males and 64 females) who met the diagnostic criteria of CAP (Community Acquired Pneumonia) were selected. A total of 145 patients were scored and divided into three groups according to the Pneumonia Severity Index (PSI), 72 cases in the low-risk group, 46 cases in the moderate-risk group and 27 cases in the high-risk group. Meanwhile, 70 people who had physical examination in the First People's Hospital of Zhengzhou City were selected as the control group. The medical records were checked to collect basic data of patients such as age, sex, body mass index, smoking history, drinking history, etc. Venous blood was collected on the day of admission (the day of physical examination in the control group) to measure the level of CRP (C-reactive protein), PCT (procalcitonin), TNF-α (tumor necrosis factor-α), Ang-2 (serum angiopoietin-2), WBC (white blood count), NLR (neutrophil to lymphocyte ratio), and PLR (platelet to lymphocyte ratio). Results: There was no significant difference in age, male proportion, BMI, smoking and drinking history between the groups of CAP patients and the control group (P＞0.05). Compared with the control group, the levels of CRP, PCT, TNF-α, Ang-2, WBC, NLR in CAP patients were significantly improved, and the difference was statistically significant (P＜0.05). With the aggravation of the condition of CAP patients, levels of CRP, PCT, TNF-α, Ang-2, WBC, NLR and PLR were significantly increased (P＜0.05). Correlation analysis results showed that the level of Ang-2 in patients was positively correlated with CRP, PCT, TNF-α, WBC, NLR, and PLR level (r=0.458 , 0.368, 0.481, 0.512, 0.419, 0.268) (P＜0.05). ROC analysis results indicated that when the optimal cutoff value of Ang-2 was 5.051, the area under the ROC curve was 0.689, the standard error was 0.023, 95 % CI (0.125-2.004), with the sensitivity of 75.32 % in predicting death of CAP patients and the specificity of 70.38 %. Conclusion: The Ang-2 level of CAP patients was significantly higher than that of the healthy people, and could be increased with the aggravation of CAP. The Ang-2 level was positively correlated with CRP, PCT, TNF- α, WBC, NLR and PLR, suggesting that the level of Ang-2 could be used to predict the condition of patients with CAP effectively.

Objective: To investigate the relationship between serum fibroblasts growth factor (FGF) 19 level and the development of atherosclerosis (AS) in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 78 T2DM patients were selected and divided into two groups based on carotid intima-media thickness (CIMT). Among them, 40 T2DM patients were control group and 38 T2DM patients with AS were experimental group. Record the blood pressure and body mass index (BMI) of all inclusive patients. Detection of related biochemical indicators such as blood sugar, blood lipids. Serum FGF19, heme oxygenase-1 (HO-1),malondialdehyde (MDA), glutathione peroxidase (GSH-Px) levels were measured by enzyme-linked adsorption immunosorbent assay (ELISA), and superoxide peroxide dismutase (SOD) level was determined by biochemical method. The differences in outcome measures between the two sets were compared. The correlations between FGF19 level and HO-1, SOD, MDA, GSH-Px, CIMT and brachial ankle pulse wave velocity (baPWV) were analyzed by Pearson correlation analysis. Multiple linear regression analysis was performed to analyze the influencing factors of FGF19. Results: Compared with the control group, CIMT, baPWV, HO-1, MDA increased in the experimental group (P＜0.01), FGF19, SOD and GSH-Px decreased significantly compared with the control group (P＜0.01). Correlation analysis showed that the serum FGF19 level of the experimental group was negatively correlated with HO-1, MDA, CIM, baPWV(r=-0.668、-0.657、-0.901、-0.734,P＜0.01) were positively correlated with SOD and GSH-px (r=0.345、0.760,P＜0.01).Multiple linear regression analysis showed that FGF19, HO-1, SOD, MDA, GSH-PX were all the possible influencing factors of CIMT (P＜0.05). Conclusion: Serum FGF19 level in T2DM patients has a certain relationship with oxidative stress, and has important clinical significance in predicting the occurrence and progression of AS.

Objective: To investigate the correlation between primary intussusception and food allergen-specific IgE, food-specific IgG and vasoactive intestinal peptide. Methods: A total of 35 children with intussusception admitted to the Fourth Hospital of Baotou from October 2021 to May 2022 were selected into the experimental group, and 35 children with no intestinal diseases at the same period were selected into the control group. The levels of 7 food allergen-specific IgE antibodies, food-specific IgG antibodies and serum vasoactive intestinal peptide of children between the two groups were compared. Results: The total positive rate of food allergen-specific IgE in children of the experimental group was 40.00 % and 28.57 % in the control group, and the differences in positive rate and total positive rate of food allergen-specific IgE of the two groups were not statistically significant (P＞0.05). The total positive rate of food-specific IgG was 88.57 % in the children of the experimental group and 62.86 % in the control group, and the difference was statistically significant (P＜0.05), but the difference in the positive rate of various types of food-specific IgG in the two groups was not statistically significant (P＞0.05). The level of vasoactive intestinal set was lower in the experimental group than that in the control group, and the difference was statistically significant (P＜0.05). The difference in serum vasoactive intestinal peptide levels between food-specific IgG-positive and negative children was statistically significant (P＜0.05), and there was a negative correlation between food-specific IgG and vasoactive intestinal peptide (P＜0.05). Conclusions: No significant correlation was seen between food allergen-specific IgE and the occurrence of primary intussusception, while food-specific IgG was correlated with the occurrence of primary intussusception. The changed levels of vasoactive intestinal peptides in the serum of children with intussusception suggest that the level of vasoactive intestinal peptide is related to the occurrence of primary intussusception, and that food-specific IgG may affect intestinal function by regulating the levels of vasoactive intestinal peptides, which ultimately lead to the occurrence of primary intussusception.

Objective: To explore the effect of the telemonitoring blood pressure management system constructed by smartphone and smart blood pressure monitor on blood pressure management of working hypertension patients. Methods: A total of 246 hypertensive patients who visited the Second Affiliated Hospital of Baotou Medical College from October 2020 to May 2021 were included, they were divided into groups according to whether they were willing to purchase smart blood pressure monitors. A total of 154 patients were included in the monitoring management group, and 92 patients who did not purchase smart sphygmomanometers were included in the routine management group. The changes in blood pressure, drug adherence and blood pressure control rate between the two groups were compared. Results: After 3 months and 6 months of management, the systolic blood pressure in the routine management group was (139.8±9.2)mmHg and (132.6±6.4)mmHg, and the diastolic blood pressure was (89.5±9.1)mmHg and (83.7±4.5)mmHg, respectively, and the systolic blood pressure in the telemonitoring management group was (131.6±10.5)mmHg and (130.3±8.0)mmHg, respectively. The diastolic blood pressure was (86.3±8.6)mmHg and (83.3±5.8)mmHg, respectively, and the systolic and diastolic blood pressure decreased in both groups as the management time was prolonged and maintained a decreasing trend (both P＜0.05). After 6 months of management, the telemonitoring management group was better than the routine management group in reducing systolic blood pressure (P＜0.05), while there was no difference in the decrease of diastolic blood pressure between the two groups (P＞0.05). After 6 months of management, the drug adherence of both groups improved, but the adherence of the telemonitoring management group was better than that of the routine management group (both P＜0.05). After 6 months of management, the blood pressure control rate increased in both groups (routine management group: 29.9 % vs. 37.0 %, telemonitoring management group: 42.9 % vs. 50.6 %), and the blood pressure control rate was better in the telemonitoring management group than in the routine management group (both P＜0.05). Conclusion: The telemonitoring management based on smartphone APP and smart sphygmomanometer can effectively reduce the systolic blood pressure of working hypertensive patients, improve the drug adherence and improve the blood pressure control rate. This model can be considered for working hypertensive patients.

Objective: To compare the short-term pain response and long-term clinical efficacy of iRoot SP and AH-PLUS as apical sealers. Methods: A total of 96 single-rooted permanent teeth of 90 patients with chronic periapical periodontitis from August 2017 to December 2018 were randomly selected as the study objects. After routine preparation of root canal, they were divided into the iRoot SP group and AH-PLUS group based on different apical sealers they used. The pain response of the two groups 1 week after surgery was compared, and clinical efficacy 6 months after surgery of the two groups were compared. Results: The pain degree in the AH-PLUS group was slightly higher than that in iRoot SP group 1 week after surgery, and the difference was statistically significant (P＜0.05). The accurate filling rate in the iRoot SP group was higher than that in the AH-PLUS group (P＜0.05). Six months after root canal filling, there was a statistically significant difference between the two groups in the treatment effect (P＜0.05). The success rate of the iRoot SP group was higher than that of the AH-PLUS group (P＜0.05). Conclusion: iRoot SP has less pain stimulation to periapical tissues 1 week after surgery, better filling effect and long-term clinical efficacy than the AH-PLUS, it could be widely used in clinical practice.

Objective: To analyze the clinical risk factors of precocious puberty in children, with the purpose of providing evidence for the prevention of precocious puberty in children. Methods: A total of 70 children with precocious puberty admitted the First Affiliated Hospital of Bengbu Medical College from July 2019 to December 2020 were selected into the observation group and 70 normal children into the control group. The general information, growth and development indicators of the two groups of children were compared. A questionnaire survey was conducted on the two groups of children’s dietary patterns, living conditions, and family status. The obtained data were analyzed using univariate analysis and multivariate logistic regression analysis. Results: There were no statistically significant differences between the two groups of children in gender, age, place of residence, and whether they were the only children(P＞0.05). Height, weight, and body mass index (BMI) of the observation group were greater than those of the control group, and the differences were statistically significant (P＜0.05). Univariate analysis showed that balanced diet, high-calorie and high-fat diet, nap habits, exercise time, sleep with light on, schoolwork burden, frequently using of plastic products and adult toiletries, watching emotional TV, mother’s menarche age, mother’s education level and occupation, parental relationship, and companionship were all related to precocious puberty in children. Multivariate logistic regression analysis showed that high-calorie and high-fat dietary patterns, mothers’ menarche age, disharmony relationship between parents, and lacking of companionship were risk factors for precocious puberty in children. Conclusion: Precocious puberty in children is affected by many factors, which are closely related to the high-calorie and high-fat diet, mothers’ menarche age, parental relationship and companionship. Family relationships should be improved to help children develop a balanced diet, nap and exercise habits, which is beneficial to children’s normal growth and development.

Objective: To explore the prognostic significance of D-dimer, routine coagulation tests and platelet parameters in pregnant women. Methods: A total of 454 cases of pregnant women who came to Guangdong Tongjiang Hospital for examination and delivery from January 2020 to June 2021 were selected as the normal pregnancy group (96 cases in the first trimester, 138 cases in the second trimester, 114 cases in the third trimester, and 106 cases in the parturient period), another 60 cases of pregnancy with other diseases such as gestation combining with diabetes or hypertension were selected as the other-disease-combining group, and 45 cases of obstetric accidents were collected as the obstetric accident group. In addition, 167 cases of non-pregnant women in childbearing age who came to our hospital for health examination were selected as the healthy control group. The D-D level and coagulation tests including activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FIB), and thrombin time (TT) were compared among the four groups. The platelet parameters including platelet count (PLT), mean platelet volume (MPV), and platelet volume distribution width (PDW) were also compared among the four groups. Results: The levels of D-D and FIB in the normal pregnancy group were significantly lower than those in the other-disease-combining group and the obstetric accident group (P＜0.05). PT level in the normal pregnancy group was significantly higher than that in the other-disease-combining group and the obstetric accident group (P＜0.05). The PLT, MPV and PDW levels in the normal pregnancy group were significantly higher than those in the other-disease-combining group and the obstetric accident group (P＜0.05). Conclusion: Four indexes of blood coagulation, D-D and platelet parameters can effectively monitor the state of blood coagulation of pregnant women before delivery, which is of great significance in mastering the coagulation condition, improving the pregnancy outcome and preventing maternal thrombosis or postpartum hemorrhage, it is worthy of popularization and clinical promotion.

Objective: To investigate the clinical efficacy of cryoablation combining with inhibitors in the treatment of epidermal growth factor receptor (EGFR) sensitive mutant lung adenocarcinoma. Methods: Clinical data of 55 patients with advanced lung cancer diagnosed and treated in the Second Affiliated Hospital of Xiamen Medical College from December 2019 to December 2020 were collected. 55 patients were divided into the combination group (n=25) and the monotherapy group (n=30) according to different treatment methods. The monotherapy group was given EGFR-TKI treatment, while the patients in the combination group were treated with cryoablation on the basis of EGFR-TKI treatment. The counts of total lymphocyte, neutrophil and platelet in routine blood test of all patients before treatment were recorded, then the optimal effect and the progression-free survival (PFS) of TKI therapy were recorded in the follow-up. Results: Overall effective rates of the two groups were not statistically different. However, the median PFS of the combination group was 18 months, which was much longer than 10.5 months of the monotherapy group (P＜0.05). Moreover, a Cox proportional hazards model was used to evaluate the potential influencing factors of PFS in TKI therapy. Lung mass greater than 4cm was the hazard factor to disease progression of patients with lung adenocarcinoma. The B value of argon-helium knife cryotherapy was 1.270, which had better benefits for the PFS. However, the diameter of mass greater than 4cm was adverse to the PFS of patients treated with TKI, given that the B value was -1.003. Further to analyze the intervention time of argon-helium knife cryotherapy, no statistical significance was found between the groups given argon-helium knife cryoablation treatment combined with TKI therapy, regardless of the use of TKI at the beginning or the lung mass getting stable after TKI treatment(P＞0.05). Conclusion: Argon-helium knife cryoablation combined with EGFR-TKI to treat EGFR-mutant lung adenocarcinoma could prolong the patients’ PFS. The diameter of lung mass greater than 4cm was the adverse factor to the PFS of patients treated with TKI.

Objective: To understand the willingness to participate elderly care of elderly people who live alone and explore its influencing factors, with the purpose of providing basis for the establishment of a robust social system of elderly care service.Methods: The data of 1 208 elderly people who live alone from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) were collected, using Andersen's model as a framework and 2 test to analyze the difference in the choice of elderly care mode among the elderly with different characteristics. Propensity, enabling resources, and needs were analyzed with binary logistic regression analysis to determine the influencing factors of willingness to participate elderly care of elderly people who live alone. Results: Among the 1 208 elderly living alone, the 92.0 % of elderly people preferred community service for home care, and only 8.0 % of elderly people willing to be admitted to institutional care for elderly. Logistic regression analysis showed that older people with an education level of elementary school, general income level, or having more than one child who live alone had lower willingness to institutional care for elderly (OR＜1,P＜0.05). The elderly living alone who were routinely cared for by related institutions, with more loneliness and poor health status, and very restricted in their ability to perform daily living, were more likely to choose an institutional care for elderly value (OR＞1, P＜0.05). The determination coefficients R² of models Ⅰ to Ⅲ increased stepwise, among which the enabling factors contributed to the most to the model. Conclusion: The willingness to participate elderly care of elderly people who live alone is mainly preferred to community service for home care. The willingness to participate elderly care of elderly people who live alone is affected by several factors, of which the enabling factors have the greatest influence. In addition to enhance community service for home care, a diverse and precise model of elderly care should be explored to meet the needs of elderly people who live alone.

Objective: To timely assess the risk of incontinence-associated dermatitis (IAD) by constructing a nomogram-predicting model for the risk prediction of IAD in critically ill patients. Method: A total of 359 ICU patients in a tertiary hospital of integrated traditional Chinese and Western medicine were selected, and divided into the nonoccurrence group (n=257) and occurrence group (n=102) according to the IAD happens or not. Univariate and multivariable logistic regression analysis were used to analysis the risk factors for IAD. A nomogram-predicting model was build based on the logistic regression model by R software. Results: Five risk factors, including fever (P=0.001,OR=3.252), the double incontinence(P=0.001,OR=3.510), daily stool frequency(P＜0.001,OR=2.987), PAT score(P=0.002,OR=1.268), and TCM syndrome differentiation nursing(P＜0.001,OR=4.540), were taken to construct a nomogram-predicting model. The model displayed good discrimination with a C-index of 0.884 and good calibration, and the predicting probability of the model was consistent with the probability of occurrence of IAD. Decision curve analysis showed that the model was clinically useful. Conclusion: This nomogram-predicting model has satisfactory prediction by combining with the theory of TCM syndrome differentiation. The risk prediction can be completed at the beginning of ICU stay, which can provide reference for preventative treatment and nursing measures for high-risk patients.

Objective: To study the effect of individualized nursing care based on risk assessment on the prognosis of cesarean section, the incidence of postoperative venous thrombosis and postpartum rehabilitation. Methods: A total of 80 parturient women who underwent cesarean section in the Third People's Hospital of Bengbu from January 2021 to April 2022 were selected and randomly divided into the routine group and the study group by random number table method. Routine intervention was performed on parturient women in the routine group, and personalized nursing care based on risk assessment was performed on parturient women in the study group. The prognosis, incidence of postoperative venous thrombosis and postpartum rehabilitation were compared between the two groups. Results: The extubation time, feeding time, lactation time and defecation time of the study group were (10.39±3.56) h, (10.39±2.16) h, (23.41±3.69) h and (26.31±5.16) h, respectively, which were significantly lower than those of the routine group (P＜0.05). The D-2 polymers, platelet levels and prothrombin time in the study group were significantly lower than those in the routine group (P＜0.05). The VAS scores at 6h, 12h and 24h of the study group were (3.71±1.03), (3.76±0.91) and (3.22±0.83) respectively, which were significantly lower than those of the routine group (P＜ 0.05). Within 1 week after delivery, lochia excretion in the study group was (80.21±15.43) mL, which was significantly lower than that in the routine group (P＜0.05), and the distance between the fundus and the umbilicus in the study group was (2.27±0.35) cm, which was significantly higher than that in the routinel group (P＜0.05). Conclusion: Individualized nursing based on risk assessment for cesarean section can effectively improve the prognosis of patients, reduce the incidence of postoperative venous thrombosis and pain degree, and help to accelerate recovery of woman after cesarean section, which is worthy of promotion.

Objective: To explore the medication rules of Mongolian medicine in the treatment of brucellosis based on data mining. Methods: The data of commonly used clinical drugs in the Department of Brucellosis of Tongliao Mongolian Medical Hospital were collected and relevant literatures on treating brucellosis with Mongolian medicine in the database of CNKI, CQVIP and WANFANG DATA from their building to now were retrieved.The prescriptions for the treatment of brucellosis were sorted out comprehensively and systematically, and the drug composition, frequency, compatibility rules of Mongolian medicine were analyzed using SPSS 25 and IBM SPSS Modeler 18. Results: In the treatment of brucellosis, the most frequently used single Mongolian medicines are gardenia, Radix Aucklandiae, Fructus Toosendan, Fructus amomi rotundus and fructus chebulae. The main properties of those medicines are warm and cold, with the main flavors of sweet, hard and bitter. The classification and analysis of viscera mainly focused on spleen, stomach and liver. The core drugs are Fructus Gardenia, Fructus Toosendan, Saffiower, Fructus amomi rotundus, cardamom, Chopper. Conclusion: In the treatment of brucellosis with Mongolian medicine, the three principles of overall stickkilling, detoxification and conditioning should be followed. The general principle is to run the black and white pulse, smooth the stiffness of muscles and bones, which is consistent with the therapeutic principles of sterilization, anti-inflammatory and immune regulation in modern medicine. Therefore, this study provides theoretical basis for the research on the mechanism of treating brucellosis with Mongolian medicine

Objective: To evaluate the quality of the systematic review and meta-analysis of acupoint application on chronic obstructive pulmonary disease (COPD). Methods: Web of Science, the Cochrane Library, EMbase, PubMed, CBM, CNKI, VIP and Wanfang Data were searched for systematic reviews/meta-analysis on acupoint application intervention for COPD, and searching time was set from inception of the above databases to April, 2022. Literatures and extracted data were screened by two researchers independently according to the inclusion and exclusion criteria. The AMSTAR 2 and GRADE were used to evaluate the methodological quality and quality of evidence. Results: A total of 9 Meta-analyses were finally included, and AMSTAR 2 evaluation results showed that the 9 papers were all of extremely low quality. The GRADE evidence classification showed that there were 14 indicators of medium quality, 18 indicators of low-level quality, and 7 indicators of extremely low-level quality among 39 outcome indicators. There was no high-level evidence were found. Conclusion: The intervention of acupoint application on chronic obstructive pulmonary disease is supported by low-quality evidence-based medicine. However, the methodological quality of systematic reviews on COPD is low, and the level of evidence quality is not high, which needs further studies to confirm this conclusion.

Objective: To investigate the antidepressive effect of Qingxin Chenxiang Bawei Powder and its effect on monoamine neurotransmitters.Methods: Kunming male mice were randomly divided into the blank control group (distilled water), positive-drug group (fluoxetine hydrochloride 0.02 g/kg) and Mongolian medicine Qingxin Chenxiang Bawei Powder groups [the high-dose (27 g/kg) group, medium-dose (9 g/kg)group and low-dose (3 g/kg) group].Intragastric administration were performed on all experimental mice.Mice tail suspension test (TST) and forced swimming test (FST) were used to evaluate the antidepressive effect of the drug 7 days after intragastric administration.After FST behavioral experiment, the contents of 5-HT, DA and NE in plasma and hippocampus were determined by enzyme-linked immunosorbent assay (ELISA).Results: Compared with the blank control group, medium-dose and low-dose groups of Mongolian medicine could significantly shorten the immobility time in mice FST, and the difference was statistically significant (P＜0.05).The immobility time in mice TST was significantly shortened in the low-dose group, and the difference was statistically significant(P＜0.05).The plasma DA level was significantly increased in the low-dose group, and the difference was statistically significant (P＜0.05).The levels of 5-HT, DA and NE in the hippocampus of mice in the low-dose, medium-dose and high-dose groups were significantly increased, and the difference was statistically significant (P＜0.05).Conclusion: Qingxin Chenxiang Bawei Powder have certain antidepressive effect on mice, which may be related to its regulation effect on monoamine neurotransmitter content.