Objective: To investigate the protective effect of ursolic acid (UA) on alcoholic liver damage (ALD) mice by observing the expression of bile salt export pump (BSEP) and sodium taurocholate cotransporting polypeptide (NTCP) in mice after UA intervention. Methods: Eighteen 10-week-old SPF C57BL/6J mice were randomly divided into three groups (normal control group, alcohol model group, and UA+alcohol group). The mice were treated with intragastric administration for 7 weeks. The changes of body weight were recorded. The pathological changes of liver tissue were observed by HE staining. The serum total bile acid (TBA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected by microplate method. The expression of BSEP and NTCP protein in liver tissue was detected by Western blotting. Results: The average body weight of mice in the alcohol group was significantly lower than that in the normal control group (P＜0.05). The structure of hepatic lobules in the normal control group was intact and there was no inflammatory cell infiltration, while the structure of hepatic lobules in the alcohol model group was blurred, accompanied by obvious steatosis, and the NAS score was significantly increased (P＜0.05). Compared with the alcohol model group, after UA intervention, the damage of hepatic lobule structure in mice was improved, the number of fat vacuoles and inflammatory cells in hepatocytes was reduced, and the NAS score was significantly reduced (P＜0.05). In addition, compared with the normal control group, the liver index of the alcohol model group was significantly increased (P＜0.05), while the liver index of the UA+alcohol group was significantly lower than that of the alcohol model group (P＜0.05). At the same time, after UA intervention, the increase of TBA, AST and ALT induced by alcohol intake was significantly inhibited (P＜0.05). The results of Western blotting showed that compared with the normal control group, the expression levels of BSEP and NTCP in the liver tissue of the alcohol model group were significantly decreased (P＜0.05). After UA intervention, the expression levels of transporter BSEP and NTCP protein in UA+alcohol group were significantly increased (P＜0.05). Conclusion: UA supplementation can significantly improve liver damage caused by alcohol, and the mechanism may be related to the up-regulation of BSEP and NTCP protein expression, thereby regulating bile acid homeostasis.

Objective: To analyze the distribution and drug resistance of pathogenic bacteria in cerebrospinal fluid infection in Baotou area of Inner Mongolia. Methods: The clinical data of 244 patients with positive cerebrospinal fluid culture from 2013 to 2024 in our hospital were collected. The samples were isolated, identified and tested for drug sensitivity, and the biochemical indexes of different pathogenic bacteria were compared. Results: Among the positive bacteria in cerebrospinal fluid culture, Gram-positive bacteria, Gram-negative bacteria and fungi accounted for 65.2%, 33.2% and 1.6%, respectively. Epidemiological studies showed that there were significant differences in the distribution of pathogens in different seasons (P＜0.05), while Staphylococcus epidermidis (16.7%) was common in summer, Staphylococcus capitis (13.4%) and Acinetobacter baumannii (11.9%) were common in autumn, and Staphylococcus aureus (10.3%) and Klebsiella pneumoniae (8.6%) were common in winter. There was no significant difference in the age distribution of pathogenic bacteria, but the content of Streptococcus pneumoniae was the higher in the age group of ≤12 years old, and the proportion of Acinetobacter baumannii was the highest in the age group of ≥60 years old. The results of drug sensitivity showed that Staphylococcus strains had no resistance to linezolid and vancomycin. Streptococcus pneumoniae had no resistance to ofloxacin and teicoplanin. Acinetobacter baumannii had no resistance to cefoperazone/sulbactam; the effect of carbapenem in the treatment of Escherichia coli was better; Klebsiella pneumoniae was sensitive to imipenem and meropenem. Conclusion: Staphylococeus epidermidis are more common in cerebrospinal fluid infections from 2013 to 2024. In addition, the significant differences in pathogens in different seasons can provide valuable strategies for disease prevention and improvement of evidence-based treatment.

Objective: To analyze the infection rate of hepatitis E virus (HEV) in different populations, and to explore the difference of HEV co-infection in different populations, so as to provide reference for early prevention and treatment of HEV co-infection in different populations, and to reduce the harm caused by HEV infection. Methods: A total of 805 serum samples were collected according to the inclusion and exclusion criteria, including 200 serum samples from normal population, 298 serum samples from pregnant women, 281 serum samples from patients with chronic liver disease (156 samples of chronic hepatitis B, 24 samples of chronic hepatitis C, 101 samples of other chronic liver diseases), and 26 serum samples from HIV-infected patients. HEV-specific IgG antibodies were detected by ELISA (capture method). Results: The positive rate of anti-HEV-IgG in HIV infected group was higher than that in normal group (P＜0.05). The age of anti-HEV-IgG positive group in HIV infected group was older than that in negative group (P＜0.05). The age of anti-HEV-IgG positive group was older than that of negative group (P＜0.05). The positive rate of anti-HEV-IgG in chronic liver disease population was higher than that in normal population (P＜0.05). In the group over 40 years old, the positive rate of anti-HEV-IgG in chronic liver disease group was higher than that in normal population (P＜0.05). Conclusion: HIV-infected patients and patients with chronic liver disease are high-risk groups for HEV infection, and the HEV infection rate is significantly higher than that of the normal population, and the risk of co-infection with HEV increases with age. Therefore, it is necessary to study the infection rate of HEV co-infection in different populations, promote multidisciplinary cooperation, effectively screen HEV-infected populations, and provide reference for early prevention and rational treatment of HEV.

Objective: To explore the value of nucleic acid detection of pathogenic bacteria in lower respiratory tract in the diagnosis of lower respiratory tract infection. Methods: Patients who underwent nucleic acid testing and bacterial culture for lower respiratory pathogens from December 2023 to November 2024 were selected as subjects. The detection rates of respiratory pathogens by both methods were compared. Results: For six common pathogens causing lower respiratory infections, the positive detection rate of nucleic acid testing was significantly higher than that of conventional bacterial culture (69.68% vs.20.88%). For Pseudomonas aeruginosa, the two methods had good consistency in positive detection rates (Kappa=0.644, P＜0.001), while the consistency of detection rate of klebsiella pneumoniae between the two methods was general (Kappa=0.473, P＜0.001), and that of staphylococcus aureus was poor (Kappa=0.287, P＜0.001). The average turnaround time within the laboratory for nucleic acid testing was 4.68 hours, while that for conventional bacterial culture was 49.75 hours, the average turnaround time of nucleic acid detection in the laboratory was significantly lower than that of bacterial culture (P＜0.001). Conclusion: Nucleic acid detection of pathogenic bacteria in lower respiratory tract has more advantages in rapid identification of pathogenic bacteria in respiratory tract, with shorter laboratory turnaround time, can provide timely information on pathogenic bacteria for clinicians, and has higher clinical significance for patients with severe and rapid progress.

Objective: To analyze the epidemiological characteristics, etiological surveillance, and clustering epidemic of hand-foot-mouth disease (HFMD) in Jinniu district of Chengdu from 2017 to 2023, so as to provide basis for the prevention and control strategy of HFMD. Methods: Descriptive epidemiological research methods were used to analyze the HFMD surveillance data in Jinniu district of Chengdu, from 2017 to 2023. Results: From 2017 to 2023, a total 12 960 cases of HFMD were reported in Jinniu district, with an annual incidence rate of 64.05/100 000 to 305.95/100 000; except for a few years, the number of began to increase in May each year, with the overall seasonal epidemic peaks occurring from May to July and from September to November. The male-to-female ratio of cases was 1.4∶1; the cases were mainly concentrated in children aged 0-＜6 years, accounting for 90.75% of the total number of cases; the most cases were scattered children, followed by children in kindergartens, accounting for 92.93% of the reported cases. The results of enterovirus monitoring showed that other enteroviruses, CVA16, and CVA6 were the main pathogens accounting for 59.75%, 15.56%, and 14.57%, respectively. The clustering epidemic was mainly concentrated from to July and from September to November, mainly occurring in kindergartens, accounting for 96.08% of the total; events with 2-5 cases were the most common, accounting for 93.56%. Conclusion: The prevention and control of HFMD should focus on young and scattered children, a particular emphasis on the changing dominant strains causing the disease. Outbreaks in Jinniu district mainly occurred in kindergartens, especially from May to and from September to November, during which targeted measures should be taken to reduce the occurrence and spread of the epidemic.

Objective: This article evaluates the biological safety of silver and stainless steel needles,which are used in Mongolian medicine.It provides scientific and technical support for their development and promotion. Methods: The experimental method followed the series of standards for biological evaluation of medical devices.Among them,Extraction standards for silver needle and stainless steel needle extracts followed the《Biological evaluation of medical devices - Part 12: Sample preparation and reference materials》(GB/T 16886.12-2023),Reference for extra-dermal and subcutaneous sensitization experiments followed the 《Biological evaluation of medical devices - Part 10: Irritation and skin sensitization tests》(GB/T 16886.10-2017),Blood compatibility test followed the《Biological evaluation of medical devices - Part 4: Selection of blood interaction tests》(GB/T 16886.4-2022),In this study, dermal and subcutaneous sensitization tests as well as blood compatibility tests were adopted to evaluate the biosafety of silver needles and stainless steel needles respectively.Male SD rats were used as experimental animals.The experimental needles were silver and stainless steel needles.The experimental solvent was needle extract and physiological saline.The general and local indicators,and the blood compatibility indicators of rats were observed in the experiment. Results: Silver and stainless steel needles did not cause obvious allergic reactions in rats in extracutaneous and subcutaneous sensitisation experiments,and did not affect the general and local indexes of rats.Silver and stainless steel needles did not cause any abnormalities such as blood coagulation,haemolysis and platelet aggregation in rats in the blood compatibility test. Conclusion: Silver and stainless steel needles are biocompatible and biologically effective,and have no significant adverse effects on the human body.They are suitable for Mongolian medical processes.

Objective: To optimize and enhance the quality standard of Yurijle-13 pill, a Mongolian medicinal preparation. Methods: :Microscopic identification was carried out on the powder of the formula encompassing Aucklandia lappa Decne, Gardenia jasminoides Ellis, Chrysanthemum morifolium Ramat, Punica granatum L, and Piper longum L in Yurijle-13 pill. Thin Layer Chromatography (TLC) was established for the identification of Aucklandia lappa Decne, Gardenia jasminoides Ellis, and Salvia miltiorrhiza Bge in Yurijle-13 pill. High Performance Liquid Chromatography (HPLC) was developed to concurrently determine the contents of the principal ingredient, Costunolide (CL), and Dehydrocostus lactone (DL) in the Aucklandia lappa Decne of Yurijle-13 pill by using a SinoPak SP analytical column (4.6 mm×250 mm, 5 μm), with a mixture of methanol and water (6535) as the mobile phase, a flow rate of 1.0 mL/min, a column temperature of 30 ℃, and a detection wavelength of 225 nm. Results: :The microscopic characteristics of the powder of Aucklandia lappa Decne, Gardenia jasminoides Ellis, Chrysanthemum morifolium Ramat, Punica granatum L, and Piper longum L in Yurijle-13 pill were distinct. Aucklandia lappa Decne, Gardenia jasminoides Ellis, and Salvia miltiorrhiza Bge demonstrated strong specificity in TLC qualitative identification. The linear regression equation for CL was y=315.83x-3.9247 (within the concentration range of 0.0051 to 0.2504 mg/mL), and that for DL was y=276.65x-5.5702 (within the concentration range of 0.0497 to 0.2494 mg/mL). The RSDs for the tests of precision, stability, and reproducibility were 1.13% and 1.17%, 3.43% and 3.04%, and 0.65% and 0.26%, respectively, and the average recovery rates were within the specified range (85.0% to 110.0%). Conclusion: :Compared with the Inner Mongolia Autonomous Region hospital preparation approved, the method for identification and content determination of Yourile-13 pill established in this study not only increase the types of identified medicinal materials and the main components of the drug, but also has the advantages of convenient operation, strong specificity and good repeatability, which is suitable for the quality control of Yourile-13 pill.

Objective: To explore the effect of glycopyrronium bromide on lung ischemia-reperfusion injury (LIRI) in rats, and to analyze the mechanism of HMGB1/TLR4/NF-κB signaling pathway in this process, so as to provide theoretical basis for clinical application. Methods: Thirty-two 6-week-old male SD rats were randomly divided into 4 different groups, with 8 rats in each group. Four groups were: sham operation group (Sham), ischemia reperfusion injury model group (I/R), low dose group (L) and high dose group (H). The pathological changes of lung tissue were observed and evaluated by HE staining. The ratio of wet weight to dry weight (W/D) was measured to determine the water content of lung tissue. The levels of indicators related to oxidative stress in lung tissue were detected. At the same time, the expression of HMGB1, TLR4 and p-NF-κB protein in lung tissue was analyzed by Western blotting, and the expression levels of HMGB1, TLR4 and NF-κB mRNA were detected by RT-qPCR. Results: Pathological findings showed that the lung tissue structure of the Sham group was clear and no damage was observed; while in the I/R group, pulmonary capillary congestion, interstitial edema, alveolar wall thickening, severe lung tissue damage, and a large number of inflammatory cell infiltration were observed. The above conditions in group L and group H were less than those in the I/R group, and H group was lighter. Compared with Sham group, the W/D ratio and the expression levels of HMGB1, TLR4 and NF-κB mRNA in the I/R group and L group were increased. However, compared with the I/R group, the performance of the above indicators in the L group and H group decreased, and the data in the H group were lower, the differences were statistically significant (P＜0.05). Further analysis showed that compared with Sham group, the concentration of malondialdehyde (MDA), the expression of HMGB1, TLR4 and p-NF-κB protein in the I/R group, L group and H group increased, while the activity of superoxide dismutase (SOD) decreased. Compared with the I/R group, the expression of the above indicators in the L group and H group decreased, while the SOD activity increased, and the performance of the H group was more obvious, the differences were statistically significant (P＜0.05). Conclusion: Glycopyrronium bromide can reduce the inflammatory response and oxidative stress during LIRI by inhibiting the activation of HMGB1/TLR4/NF-κB signaling pathway, thereby improving LIRI in rats.

Objective: To observe the effect of Mongolian medicine total flavonoids of Dracocephalum moldavica L (TFDM) on the expression of microtubule-associated protein-2 (MAP-2) in the ischemic penumbra of rats after cerebral ischemia-reperfusion, and explore its role in neural plasticity. Methods: Constructing a middle cerebral artery occlusion reperfusion model using SD rats. The experiment was divided into sham surgery group (Sham), ischemia-reperfusion group (I/R), and TFDM administration group (TFDM). Samples were collected at four time points: 1, 3, 7, 14 days after surgery. The immunohistochemical staining was used to observe the expression of MAP-2 positive labeled cells and dendrites in the ischemic penumbra of the cortex, and Western blot was used to detect the expression of MAP-2 protein in the ischemic penumbra of the cortex. Results: The results of immunohistochemistry and Western Blot showed that the expression of MAP-2 in the ischemic penumbra of the TFDM group was significantly higher than that in the I/R group on the 3rd and 7th day after reperfusion (P＜0.05). Conclusion: TFDM may play a neuroprotective role by up-regulating the expression of MAP-2 protein, enhancing the regulation of neural plasticity in the ischemic penumbra, promoting dendritic growth and repairing damaged nerve structure.

Objective: To analyze the proportion of SPP1⁺ macrophages in peripheral blood of patients with connective tissue disease complicated with interstitial lung disease (CTD-ILD) and the expression level of its expression product chemokine 18 (CCL18) in serum of patients with CTD-ILD and its clinical significance. Methods: Twenty patients with connective tissue disease (CTD) and 20 healthy people were selected as the control group, and 35 patients with newly diagnosed CTD-ILD were selected as the experimental group. The differences in the expression levels of SPP1⁺ macrophages and CCL18 in the experimental group and the control group were compared, and the correlation with type I collagen (COL1A1), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) was analyzed. Results: Compared with the control group, the proportion of SPP1⁺ macrophages in peripheral blood mononuclear cells of CTD-ILD patients was significantly increased (P＜0.05), and the content of CCL18 in serum was also significantly increased (P＜0.05). At the same time, SPP1⁺ macrophages and CCL18 were positively correlated with ESR, CRP, COL1A1 and lung imaging score (HRCT score) (P＜0.05), and negatively correlated with the percentage of forced vital capacity (FVC%) and the percentage of carbon monoxide diffusion (DLCO%) (P＜0.05). The area under the curve (AUC) of SPP1⁺ macrophages and CCL18 were 0.732 and 0.939, respectively. The AUC of the combined detection of SPP1⁺ macrophages and CCL18 was 0.939, the sensitivity was 93.5%, the specificity was 83.3%, and the Youden index was 0.768. Conclusion: SPP1⁺ macrophages and CCL18 are poor prognostic factors in patients with CTD-ILD. The combined detection of SPP1⁺ macrophages and CCL18 is of great significance for the prognosis of the disease.

Objective: To analyze the expression level and the correlation with clinical data of type X collagen α 1 chain (COL10A1) in the serum of breast cancer patients, so as to evaluate its predictive value for breast cancer risk. Methods: GEPIA database was used to analyze the expression of COL10A1 in breast cancer patients. The expression of COL10A1 in the serum of 30 breast cancer patients was detected by PCR, and its relationship with the clinical data of patients was analyzed. ROC curve was used to analyze the clinical diagnostic value of COL10A1 in breast cancer. The potential targets of COL10A1-mediated breast cancer were obtained through the Genecards database and OMIM database, and they were input into the STRING database to obtain the TSV file of the protein-protein interaction (PPI) network. GO enrichment analysis and KEGG enrichment analysis were used to explore different functional categories and related signal transduction pathways. Results: Bioinformatics analysis and PCR results showed that COL10A1 was highly expressed in breast cancer, which was closely related to histological grade, TNM stage, distant lymph node metastasis and Her2. ROC curve showed that the area under the curve of COL10A1 was 0.690 0 (95%CI: 0.5567-0.8233). When the optimal cut-off value was 1.1026, the sensitivity and specificity of COL10A1 in the diagnosis of breast cancer were 60.0% and 68.9%, respectively. A total of 14 potential targets of COL10A1-mediated breast cancer were obtained by combining the Genecards database with the OMIM database. A total of 241 GO terms were enriched by GO analysis, including 9 cellular components, 48 biological processes, and 12 molecular functions. KEGG enrichment analysis enriched a total of 12 signal transduction pathways. Conclusion: The high expression of serum COL10A1 in breast cancer patients is closely related to clinicopathological indicators, and has certain clinical value for breast cancer risk assessment.

Objective: To observe the efficacy and safety of bidirectional subintimal angioplasty under fluoroscopy in the treatment of TASC Ⅱ C and D lesions of lower extremity arteriosclerosis obliterans (ASO). Methods: The clinical data of 22 patients with TASC Ⅱ grade C and D (24 limbs in total) ASO who underwent bidirectional subintimal angioplasty in the Department of Interventional Vascular, Hefei Second People's Hospital from June 2018 to June 2021 were collected. The success rate of operation, the incidence of complications in hospital and the improvement rate of ankle brachial index (ABI) after operation were statistically analyzed. Results: A total of 23 limbs were successfully operated, and 1 limb failed to open the occluded segment after repeated attempts of antegrade and retrograde arteries of the lower leg, with a success rate of 95.8%. The incidence of postoperative complications was 30.4%, and the mortality rate was 0.0%. The postoperative ABI was (0.79±0.16), which was better than(0.27±0.19) of the preoperative ABI (P＜0.05). The 6-month target vessel patency rate was 91.3% (21/23). There was no new amputation within 6 months. Conclusion: Bidirectional subintimal angioplasty is a safe and convenient endovascular treatment method. It is an effective remedy for long-segment and severe lower extremity arterial occlusive lesions that cannot be recanalized.

Objective: To analyze the correlation between the levels of serum transcription activator factor 3 (ATF3), tumor necrosis factor receptor 1 (TNFR1), angiopoietin-like protein 4 (ANGPTL4) and the renal function, prognosis of patients with diabetic nephropathy. Methods: A total of 152 patients with diabetic nephropathy who were treated in the Second People's Hospital of Jiaozuo City from April 2022 to January 2024 were selected as the study group, and 152 patients with simple diabetes who were treated in the same period were selected as the control group. The levels of serum ATF3, TNFR1, ANGPTL4 and renal function indexes such as serum calcium, serum phosphorus, serum creatinine and glomerular filtration rate (eGFR) were compared between the two groups at admission, and their correlation was analyzed. The levels of serum ATF3, TNFR1 and ANGPTL4 in patients with different prognosis were compared at admission, and their correlation with prognosis and predictive value for prognosis were analyzed by partial regression. Results: Compared with the control group, the levels of ATF3, TNFR1, and ANGPTL4 in the study group were significantly higher at admission (P＜0.05). The levels of blood calcium and creatinine in the study group were higher than those in the control group, while the levels of blood phosphorus and eGFR were lower than those in the control group (P＜0.05). Serum ATF3, ANGPTL4, and TNFR1 were negatively correlated with blood phosphorus and eGFR, and positively correlated with blood calcium and creatinine levels (P＜0.05). In the study group, patients with poor prognosis had higher levels of ATF3, ANGPTL4, and TNFR1 on admission than patients with good prognosis (P＜0.05). Logistic regression analysis showed that serum ATF3, ANGPTL4 and TNFR1 were risk factors for poor prognosis of patients (P＜0.05). The efficacy of combined prediction of serum ATF3, TNFR1 and ANGPTL4 levels on admission in predicting poor prognosis of patients was 0.807, which was higher than that of each index alone (P＜0.05). Conclusion: The increase in serum levels of ATF3, TNFR1, and ANGPTL4 in patients with diabetic nephropathy is associated with their renal function. Clinical detection of these levels can help predict the prognosis of patients and provide effective reference for clinical diagnosis and treatment.

Objective: To investigate and analyze the efficacy of high-flow nasal cannula oxygen therapy in the sequential treatment of mechanical ventilation weaning for pneumonia complicated with type I respiratory failure. Methods: A total of 70 patients with pneumonia and type I respiratory failure admitted from January 2023 to December 2024 were divided into experimental group and control group. All patients were treated with mechanical ventilation. The experimental group was treated with high-flow nasal cannula oxygen therapy, and the control group was treated with non-invasive positive pressure ventilation. The clinical efficacy, blood gas analysis indexes (before treatment, 12, 24 and 48 hours after treatment) and sputum traits, inflammation index levels (before treatment and 3 days after treatment), sequential treatment time, total hospitalization time and reintubation mechanical ventilation rate were compared between the two groups. Results: The total effective rate of clinical efficacy in the experimental group was higher than that in the control group (P＜0.05). There were no statistically significant differences in PaO₂, PaCO₂, and SaO₂ levels between the two groups before treatment and at 12, 24, and 48 hours after treatment (P＞0.05). There was no significant difference in PaO₂, PaCO₂ and SaO₂ levels between the two groups before treatment and 12,24 and 48 hours after treatment (P＞0.05). There was no difference in CRP and PCT levels between the two groups before treatment (P＞0.05). After 3 days of treatment, CRP and PCT in the experimental group were lower than those in the control group (P＜0.05). There was no significant difference in sputum traits between the two groups before treatment (P＞0.05). After 12, 24 and 48 hours of treatment, the sputum traits I of the experimental group were better than those of the control group (P＜0.05). The sequential treatment time and total hospitalization time of the experimental group were lower than those of the control group (P＜0.05). Conclusion: High-flow nasal cannula oxygen therapy can improve the clinical efficacy of patients with pneumonia complicated with type I respiratory failure after mechanical ventilation weaning, dilute sputum, improve oxygenation, reduce inflammation, improve patient tolerance, reduce the risk of re-intubation mechanical ventilation due to intolerance, shorten the treatment time, and promote its rehabilitation. It is worthy of application.

Objective: To investigate the value of serum and gingival crevicular fluid myeloid cell-associated protein-8/14 (MRP-8/14), Toll-like receptor 4 (TLR4), Toll-like receptor 9 (TLR9) in evaluating periodontal-orthodontic efficacy in periodontitis patients with malocclusion. Methods: A total of 72 periodontitis patients with malocclusion admitted to Yongcheng People's Hospital from January 2022 to July 2023 were selected as the observation group, and 72 healthy subjects in the same period were selected as the control group. The levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid were compared between the two groups before treatment. The indexes of serum and gingival crevicular fluid, inflammatory response indexes [high-sensitivity C-reactive protein (hs-CRP), soluble intercellular adhesion molecule (sICAM-1), monocyte chemoattractant protein-1 (MCP-1)] and periodontal indexes [plaque index (PLI), bleeding index (SBI), probing depth (PD)] were compared between the two groups before treatment and 3 months after treatment. The relationship between the indexes of serum and gingival crevicular fluid and the indexes of inflammatory response and periodontal indexes and the evaluation value were analyzed. Results: Before treatment, the levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid in the observation group were higher than those in the control group(P＜0.05). The levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid of patients with good efficacy and poor efficacy were significantly lower than those before treatment after 3 months of treatment (P＜0.05). The levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid of patients with good efficacy were significantly lower than those of patients with poor efficacy before treatment and after 3 months of treatment (P＜0.05). The levels of SBI, PLI, PD, serum hs-CRP, sICAM-1 and MCP-1 in patients with good efficacy before treatment in the observation group were lower than those in patients with poor efficacy. MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid were positively correlated with hs-CRP, PLI, sICAM-1, SBI, MCP-1 and PD (P＜0.05). The AUC of combined evaluation of serum and gingival crevicular fluid indexes before treatment and 3 months after treatment was the largest (P＜0.05). Conclusion: The high expression of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid of periodontitis patients with malocclusion is related to inflammatory response, periodontal index and periodontal-orthodontic efficacy. The combined prediction of periodontal-orthodontic efficacy has a high reference value.

Objective: To investigate the effects of oral nutrition supplementation 10 days before surgery on preoperative nutritional status, immune function and postoperative infectious complications in patients with oral cancer. Methods: A total of 40 elderly patients with preoperative oral cancer who were screened by Nutritional Risk Screening 2002 (NRS-2002) score were divided into observation group and control group according to random number table method, with 20 patients in each group. The two groups were given dynamic nutritional support according to the target demand of energy and protein after operation. The control group was given routine nutritional education before operation. The observation group was given Nengquansu combined with whey protein powder oral nutrition supplement on the basis of the control group. The changes of nutritional status indexes, immune function indexes and postoperative infectious complications before and after intervention were compared between the two groups. Results: After intervention, the levels of albumin and prealbumin in the observation group were significantly higher than those in the control group, and the differences were statistically significant (P＜0.05), while there was no significant difference in RBC, HB between the observation group and the control group (P＞0.05). After intervention, IgA, CD4 and CD4/CD8 of the observation group were significantly higher than those of the control group, while C-reactive protein of the observation group was significantly lower than that of the control group, the differences were statistically significant (P＜0.05). After intervention, there was no significant difference in IgG, IgM, CD3, CD8 and NK cells between the observation group and the control group (P＞0.05). After intervention, the proportion of the total number of complications in the observation group was significantly lower than that in the control group, and the difference was statistically significant (P＜0.05). Conclusion: The preoperative oral nutritional supplement of Nengquansu combined with whey protein powder can improve the preoperative nutritional status and immune function of patients with oral cancer, and reduce the incidence of postoperative infectious complications.