Objective: To investigate the changes of DNA methyltransferase in microglia after hypoxia. Methods: BV2 cells were used as the research object, and they were divided into the control group, H-0 group (hypoxia for 12 h without reoxygenation), H-2 group (hypoxia for 12 h with reoxygenation for 2 h), H-4 group (hypoxia for 12 h with reoxygenation for 4 h), H-8 group (hypoxia for 12 h with reoxygenation for 8 h), H-12 group (hypoxia for 12 h with reoxygenation for 12 h), and H-24 group (hypoxia for 12 h with reoxygenation for 24 h). The BV2 cells were cultured under hypoxia condition for 12 h and reoxygenated at different time points to construct the model, and the cell morphology was observed in the bright field by fluorescence inverted microscope. The expression changes of DNMT1, DNMT3A, and DNMT3B at the mRNA level were detected by Real-time PCR, and the expression changes of DNMT1, DNMT3A, and DNMT3B at the protein level were detected by Western blot. Results: Compared to the Control group, the H-0 group had a decreased number of cells, wrinkled cell morphology, and elongated tentacles in most cells. The cells were dull and the refractive index was reduced. With the reoxygenation time increasing (H-2, H-4, H-8, H-12, H-24), the adherent cells gradually decreased. Compared with the control group, the protein expression level of DNMT1 was significantly increased in the H-0 group (P＜0.05), and the mRNA and protein expression level of DNMT1 was significantly decreased in the H-8, H-12, and H-24 groups (P＜0.05). mRNA and protein expression level of DNMT3A was significantly decreased in the H-8, H-12 groups (P＜ 0.05). mRNA and protein expression of DNMT3B in cells of H-0, H-4, H-8, H-12, and H-24 groups did not change significantly. Conclusion: DNMT1 and DNMT3A play important roles in microglia after hypoxia.

Objective: To investigate the effects of neodymium nitrate [Nd(NO₃)₃] on chorionic cell apoptosis and the changes of microRNA expression in small for gestational age (SGA) infants, aiming to explore the molecular mechanisms of neodymium inducing SGA. Methods: The human trophoblast cell line (JEG-3) was selected. The optimal dose and time of Nd(NO₃)₃ exposure were determined by CCK-8 assay. Apoptosis was measured using flow cytometry. MicroRNA (miRNA) was extracted from the control group (0 μg/mL) and experimental group treated with low, medium, and high doses (0.2, 2, 20 μg/mL) of Nd(NO₃)₃. The expression levels of miR-15a, miR-466b-3p, miR-328-3p, and miR-144-5p were measured by qPCR. After transfection with miR-15a and miR-144-5p inhibitors, transfection efficiency was evaluated, and CCK-8 and flow cytometry assays were repeated to confirm their effects on apoptosis. Results: The CCK-8 assay showed that cell viability in the medium-dose group decreased to 75.53%, and Flow cytometry also indicated increased apoptosis in this group. After 24 hours of Nd(NO₃)₃ exposure, qPCR analysis showed that miR-15a, miR-466b-3p, miR-328-3p, and miR-144-5p were upregulated compared to the control group. After JEG-3 cell line transfected with the above microRNA inhibitors, the survival rate of miR-144-5p and miR-15a inhibitor transfected group in CCK-8 medium-dose group increased from 75.53% to 76.71% and 83.03%, respectively, while the apoptosis level decreased from 18.70% to 18.44% and 15.34% respectively. Conclusion: The toxic effect of [Nd(NO₃)₃] on cells is dose-dependent. With increased expression levels of miR-15a, miR-466b-3p, miR-328-3p, and miR-144-5p, downstream signaling pathway is activated and apoptosis mechanism is regulated, which plays an important role in cell stress response induced by (Nd(NO₃)₃). This study provides a new research direction for exploring the molecular mechanism of SGA.

Objective: To investigate the potential mechanisms of nonsteroidal anti-inflammatory drugs (NSAIDs) as anti-inflammatory analgesics against respiratory syncytial virus (RSV) through a network pharmacology approach. The most influential NSAIDs against RSV were screened through network pharmacology studies. Methods: NSAIDs (19 active drugs and one prodrug) approved by U.S. Food and Drug Administration (FDA) were used in this study. Targets for NSAIDs were collected through the Swiss Target Prediction and PubChem databases, and RSV targets were searched in the GeneCards database. The intersection of NSAIDs target proteins with RSV target proteins was mapped using Wayne's Web, protein interaction analysis of drug-disease intersection target proteins was performed in the STRING database, and the network was embellished using Cytoscape 3.9.0. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the intersecting target proteins was performed in the DAVID database. Bubble plotting for KEGG pathway enrichment analysis was made using the Bioinformatics drawing tool. Pathway-target-component network analysis was performed by Cytoscape 3.9.0. Finally, the binding affinity of NSAIDs to target proteins is determined by molecular docking (AutoDock Vina). Results: A total of 421 targets of NSAIDs, 391 targets associated with RSV, 39 overlapping target proteins were collected. 37 anti-RSV signaling pathways were revealed busing Genomic enrichment analysis. MAPK1, AKT1 and MAP2K1 were explored as the target proteins with the greatest relevance to the B-cell receptor signaling pathway. Conclusion: Three NSAIDs (sulforaphane, rofecoxib and diclofenac) may block the B-cell receptor signaling pathway by inactivating their related target proteins, thereby potentially alleviating RSV-induced bronchitis and pneumonia.

Objective: To investigate the expression of CD34 and D2-40-labeled carcinoembryonic antigen M2A in COAD tissues and its significance. Methods: The expression of CD34 and D2-40-labeled M2A protein was detected in 60 cases of COAD and 4 cases of adjacent normal tissues by immunohistochemical Elivision^TM plus method, and the density of CD34-positive microvessels and D2-40-positive microlymphatic vessels were counted respectively, and its relationship with COAD clinicopathological parameters were analyzed. Results: In COAD tissues, microvessel density and microlymphatic vessel density of positive CD34 and D2-40 were significantly higher than those in adjacent normal tissues. The CD34 microvessel density in COAD tissue was related to the lesion site, the CD34 microvessel density in patients with right colon cancer was significantly higher than that in left colon cancer patients(P＜0.05). CD34 microvessel density was positively correlated with D2-40 microvessel density in COAD tissues combining with mesenteric lymph node metastasis. Conclusion: CD34 and D2-40 labeled M2A protein are highly expressed in COAD tissues, and they are positively correlated with mesenteric lymph node metastasis, suggesting that they may be synergistically involved in the occurrence of COAD lymph node metastasis.

Objective: To evaluate the clinical value of serum tumor markers in the diagnosis and classification of lung cancer. Methods: A total of 119 patients with lung cancer admitted to the Department of Respiratory and Critical Care Medicine of the First Affiliated Hospital of Bengbu Medical College from January to October 2023 were selected as the study group, and 54 patients with benign lung tumors in the same period were selected as the control group. The content of serum tumor markers carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC), cytokeratin fragment (CYFRA21-1), neuron-specific enolase (NSE) and gastrin-releasing peptide precursor (ProGRP) in each group was detected and the diagnostic efficacy was analyzed. Results: The serum tumor markers in the study group were higher than those in control group (P＜0.05). The CEA content of adenocarcinoma group was higher than that of squamous cell carcinoma group and small cell carcinoma group (P＜0.05), the contents of SCC and CYFRA21-1 in squamous cell carcinoma group were higher than those in the adenocarcinoma group and small cell carcinoma group (P＜0.05), the levels of NSE and ProGRP in the small cell carcinoma group were higher than those in the adenocarcinoma group and squamous cell carcinoma group (P＜0.05). The AUC of CEA detection for adenocarcinoma was 0.987, that of SCC and CYFRA21-1 detection for squamous cell carcinoma was 0.994 and 0.957, and that of NSE and ProGRP detection for small cell carcinoma was 0.923 and 0.944. The AUC (0.991, 0.997, 0.996) of the combined tests for adenocarcinoma, squamous cell carcinoma and small cell carcinoma were higher than those in separate tests (P＜0.05). Conclusion: Serum tumor markers have a high guiding value in the definite diagnosis of lung cancer and the differentiation of pathological types. CEA has a high diagnostic value in adenocarcinoma, as well as SCC and CYFRA21-1 in squamous cell carcinoma, and NSE and ProGRP in small cell carcinoma. The combination of the above serum markers can improve the diagnostic efficiency of different pathological types of lung cancer.

Objective: To investigate the effect of prone kneeling position in the second stage of labor on the delivery outcome of primipara who underwent labor analgesia. Methods: Base on the inclusion and exclusion methods, the primiparas who underwent labor analgesia were randomly divided into the observation group and the control group with 95 cases in each group. In the observation group, the prone kneeling position was adopted at the beginning of the second stage of labor until the fetus was exposed (S+4), and then the bladder lithotomy position was instead. The bladder lithotomy position was adopted in the control group for the whole of delivery.The women in both groups were delivered under conventional ways. The differences of labor and delivery outcomes between the two groups were analyzed and compared. Results: The second stage of labor in the observation group was shorter than that in the control group , and the one-minute Apgar score of newborns in one minute was higher than that in the control group. Binary logistic regression analysis showed that the vaginal delivery rate in the observation group was significantly higher than that in the control group (OR=8.644,95%CI 1.059-70.530), and the incidence of fetal deceleration during the second stage of labor was significantly lower than that in the control group (OR=0.253,95%CI 0.079-0.809). Conclusion: For primiparas who under going labor analgesia throughout the whole process of delivering, the application of prone kneeling position in the second stage of labor can shorten the second stage of labor, reduce the rate of conversion to cesarean section, increase the success rate of vaginal delivery, and improve Apgar score of newborns.

Objective: To study the structure and function of pelvic floor using 4D perineal ultrasonography, and to provide quantitative parameters and objective basis for the diagnosis of early postpartum stress urinary incontinence (SUI). Methods: A total of 30 women with SUI symptoms 42 to 100 days after dilivery were collected from the Second Affiliated Hospital of Baotou Medical College as the case group (SUI group), and 30 women without SUI symptoms in the same period as the control group (non-SUI group). Bladder neck mobility (BNM), urethral rotation angle (URA) and posterior bladder angle (RVA) were measured under different conditions, and the strain rate of levator ani muscle was calculated for analysis and comparison. The cut-off value of ultrasonic diagnosis was obtained by drawing the receiver operating characteristic curve (ROC). Results: There was no significant difference in general data between the two groups (P＜0.05). The area shrinkage strain rate (εA1) in both SUI and non-SUI groups was positively correlated with the perimeter shrinkage strain rate (εC1) (r=0.617, P＜0.01;r=0.457, P＜0.05). The εC1 of both groups was positively correlated with the strain rate of diameter shrinkage (εL1) (r=0.569, P＜0.01;r=0.563, P＜0.01). The average area tensile strain rate (εA2) of the two groups was positively correlated with perimeter tensile strain rate (εC2) (r=0.370, P＜0.01;r=0.647, P＜0.01) and tensile strain rate (εL2) (r=0.663, P＜0.01;r=0.427, P＜0.01). εC2 in the two groups was both positively correlated with εL2 (r=0.785, P＜0.01;r=0.542, P＜0.01). The ROC curve showed that εA1 had the best diagnostic cutoff, the largest area under the curve (0.993 6), and the highest sensitivity (97.4%). Conclusion: The strain rate of levator ani muscle contraction is valuable in the diagnosis of early postpartum SUI.

Objective: To explore the surgical experience and technical optimization of mortise and tenon bone grafting in repairing tibial defect in severe knee osteoarthritis. Methods: A total of 50 patients of the First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology with tibial plateau defect for severe knee osteoarthritis were repaired by mortise and tenon bone graft. Results: All surgeries successfully completed without serious complications. All patients were followed-up for 6 months to 11 years, with an average follow-up of 9.3 years. The postoperative KSS score was 84-92, with an average of 88 scores, which was significantly improved compared with that before surgery. Good knee range of motion were found in all patients at the last follow-up. In the last follow-up, the imaging results showed that defects of the tibial plateau bone were effectively repaired, the knee prosthesis was in good position and of reliable bearing function, and all the bone grafts were completely fused with the tibia without bone resorption, osteonecrosis or other complications. In the last follow-up, for patients with large defects of the tibial plateau, the defect of the tibial plateau was repaired by increasing the osteotomy angle combined with pressure bone grafting, and the transplanted autogenous bone in all patients was completely integrated into the host bone without any clinical symptoms. Conclusion: Mortise and tenon bone grafting in repairing tibial plateau defect for severe knee osteoarthritis is more compact and stable in the early stage. By further improving the surgical details, the contact gap between bone fragments and main bone is smaller and the scope of adaptation is wider, which is a good surgical method and worthy of promotion.

Objective: To investigate the efficacy of desonide cream combined with Heling emollient in the treatment of mild to moderate atopic dermatitis in infants. Methods: A total of 60 infants with atopic dermatitis treated in our hospital from June 2022 to June 2023 were randomly divided into the control group (n=30) and the observation group(n=30). The control group was treated with desonide cream alone, the observation group was treated with desonide cream combined with Heling emollient. Scoring atopic dermatitis (SCORAD) before and after treatment and the clinical efficacy were compared between the two groups of patients. Results: After 2 weeks of treatment, the SCORAD scores and clinical efficacy of two groups were both improved, and the SCORAD score and effective rate in the observation group were better than those in the control group (P＜0.05). The incidence rate of adverse reactions in the observation group and the control group was 3.33% and 16.66% respectively (P＞0.05). Conclusion: Desonide cream combined with Heling emollient can significantly reduce clinical symptoms and improve the therapeutic effect in infants with mild to moderate atopic dermatitis.

Objective: To investigate the prevalence of metabolic syndrome (MS) and Helicobacter pylori (Hp) infection, and to analyze the relationship between MS and Hp infection, uric acid (UA). Methods: A total of 305 patients with HP infection and UA were selected from the Zhangzhou Affiliated Hospital of Fujian Medical University from January 2022 to September 2022, they were divided into the MS Group and non-MS Group, HP (+) group and HP (-) group. BP and blood glucose were compared between groups. Binary logistic regression model was used to analyze the risk factors of MS and the relationship between Hp infection and age and sex. Results: The incidence of MS and Hp infection was 15.41% and 44.92% respectively. There were significant differences in age, male ratio, Hp infection rate, UA, BMI, TG, blood glucose, BP and HDL-C between the MS Group and the non-MS Group (P＜0.05). There were significant differences in age, MS ratio, UA, BMI, overweight, TG, hypertriglyceridemia, BP, hypertension, HDL-C and low HDL-C between Hp (+) group and Hp (-) group (P＜0.05). Age was an independent risk factor for Hp infection. Hp infection, male gender, HDL-C, blood glucose, DBP and BMI were independent risk factors for MS. Conclusion: The incidence of MS in male is higher than that in female. The relationship between Hp infection and sex is not obvious, but the infection rate increases with higher age. Hp infection and male are independent risk factors for MS, while UA was not.

Objective: To investigate the clinical characteristics and risk factors of severe acute pancreatitis (SAP) combined with invasive fungal infections (IFI). Methods: The clinical data of 84 SAP patients admitted from January 2019 to December 2021 were retrospectively analyzed and divided into the IFI group (25 cases) and the non-IFI group (59 cases) according to whether or not IFI infection occurred. The types and sites of fungal infections in patients in the IFI group were analyzed, and the clinical differences between the IFI and non-IFI groups were compared and analyzed by logistic regression. Results: There were 25 patients among the 84 SAP patients combining with IFI, with an incidence of 29.76%. 8 cases of death occurred in these patients, with a mortality rate of 32.00%. A total of 31 fungal strains were detected in pathogenetic examination, all of which were Candida. Among them, 15 strains of Candida albicans (48.40%), 6 strains of Candida near-smooth (19.35%), 6 strains of Candida smooth (19.35%), 2 strains of Candida tropicalis (6.45%), and 2 strains of Candida kriegerii (6.45%) were detected. The sites of IFI in SAP patients were lung (80.64%), abdominal cavity (6.45%), blood (6.45%), biliary tract (3.23%), and intestine (3.23%). The results of univariate analysis showed that the combination of IFI in SAP patients was associated with age, lymphocyte count (×10⁹/L), procalcitonin(PCT) (ng/mL), multiple organ dysfunction syndrome (MODS), shock, APACHE II score, total parenteral nutrition (TPN) time (d), antibiotic using time (d), number of types of antibiotics used, indwelling time of urinary catheter (d), indwelling time of deep venous catheter (d), mechanical ventilation time (d) and length of ICU stay(h). Multivariate analysis results showed that age, PCT, indwelling time of deep venous catheter (d), mechanical ventilation time (h), and length of ICU stay (h) were independent risk factors of SAP patients complicated with IFI (P＜0.05). Conclusion: The strain distribution of SAP combined with IFI was mainly candida albicans, and the main site of prevalence was the lungs. Age, PCT, duration of deep venous catheterization, mechanical ventilation time, and length of ICU stay were independent risk factors for SAP patients complicated with IFI.

Objective: To measure the thickness of fetal nuchal translucency (NT) by ultrasound in early pregnancy and explore the correlation between NT and fetal prognosis. Methods: A total of 2 779 pregnant women who underwent NT thickness measurement in early pregnancy from January 2017 to January 2019 were selected as subjects. According to the results of fetal NT thickness measurement, they were divided into NT thickening group (194 cases) and NT normal group (2 585 cases). The relationship between different NT thickening amplitude groups and abnormal fetuses, and the relationship between different NT thickening amplitude groups and adverse pregnancy outcomes were analyzed. Results: The incidence of adverse pregnancy outcomes in the NT thickening group was 30.93%, which was higher than 2.36% in the NT normal group, and the difference was statistically significant (P＜0.05). There was a significant difference in the incidence of adverse pregnancy outcomes between groups with different NT thickness (P＜0.05), and the incidence of adverse pregnancy outcomes increased with the increase of NT thickness. In the elderly pregnant women with NT thickening, the incidence of fetal adverse pregnancy outcomes among different age groups was statistically significant (P＜0.05), and the ratio of fetal adverse pregnancy outcomes increased with the age of pregnant women. Conclusion: The thickening of transparent layer of fetal neck can increase the incidence of adverse pregnancy outcomes. Ultrasound screening of fetal NT thickness in early pregnancy can improve the screening rate of fetal malformations, especially the detection rate of multiple structural malformations. When NT thickening is combined with advanced age, it will further improve the rate of predicting adverse pregnancy outcomes, which is worthy of clinical application.

Objective: To systematically evaluate the influencing factors of depression and anxiety among empty nesters in China. Methods: The Chinese and English databases were searched from the inception to July 2022. The literature screening,quality assessment,and information extraction were performed by 2 researchers independently. RevMan 5.4 and Stata 15.0 were used for Meta-analysis. Results: A total of 22 studies with 34 911 participants were included. Among them,the related factors of depression were reported in 19 studies,and the related factors of anxiety in empty nesters were reported in 7 studies. The Meta-analysis suggested that the risk factors for depression among empty nesters were living without spouse [OR=1.39(1.21,1.59)],low frequency of children’s visits [OR=1.49(1.34,1.67)],living in rural areas[OR=1.40(1.22,1.62)],religious belief [OR=2.66(1.68,4.22)],poor self-rated health status [OR=2.16(1.80,2.58)],comorbid chronic diseases [OR=1.39(1.14,1.69)],physical pain [OR=2.09(1.62,2.70)],poor self-care ability in life [OR=2.01(1.22,3.32)],multiple somatic disorders [OR=1.98(1.71,2.29)],and poor economic status [OR=2.43(1.96,3.02)]. The protective factors for depression among empty-nesters were high education level [OR=0.80(0.71,0.90)],living no alone [OR=0.61(0.39,0.95)],physical exercise [OR=0.42(0.29,0.61)],drinking [OR=0.52(0.34,0.97)],and high social support [OR=0.96(0.94,0.98)]. Gender and age were not associated with depression among empty nesters(P＞0.05). In addition,the risk factors for anxiety among empty nesters were poor economic status [OR=1.80(1.49,2.19)],living without spouse [OR=1.88(1.35,2.64)],religious belief [OR=2.39(1.74,3.27)],and multiple somatic disorders [OR=2.81(2.05,3.85)]. No significant associations were found among chronic diseases and anxiety in empty nesters(P＞0.05). Conclusion: Numerous factors that contribute to depression and anxiety among empty nesters in China. Therefore,preventive measures should be timely taken to lower incidence of depression and anxiety in empty nesters of China.

Objective: To explore the current situation and influencing factors of college students' health emergency literacy. Methods: The three-stage cluster sampling were used for stratified cluster random sampling, and college students majoring in arts, science and medicine in three universities were surveyed with self-designed questionnaire on health emergency literacy. Chi-square test and multi-level Logistic regression model were used for statistical analysis. Results: The average score of health emergency literacy knowledge and skills among the selected college students was (56.68±6.50),52.63% of which having basic health emergency literacy scores ≥60.Among the five aspects in the questionnaire, scores on nuclear and radiation incidents had the highest pass rate (90.64%), while the average score and pass rate of general knowledge applicable to various incidents were lower. The pass rate of female college students was higher than that of male in health emergency literacy awareness, infectious disease, and emergency medical rescue (P＜0.05). In health emergency literacy awareness, infectious disease, emergency medical rescue, and group acute poisoning events, the scores was statistically different among different age groups (P＜0.05). There was no significant difference in health emergency literacy between college students with agricultural household registration and non-agricultural household registration (P＞0.05). Multivariate logistic regression analysis results showed that those of 19-21 years old(OR=3.069)or with average monthly household income of 1000-1999 Yuan(OR=8.834)had lower health emergency literacy, while college students from minority nationality(OR=0.150)had higher health emergency literacy. Conclusion: The health emergency literacy level of college students, especially the general knowledge applicable to various events, needs to be improved. Further measures should be taken to improve the health emergency literacy of college students.

Objective: To explore Hawthorne effect on the hand hygiene compliance of health care workers (HCWs) in intensive care unit, and to provide reference for the rational use of Hawthorne effect to strengthen the hand hygiene management in medical institutions in the future. Methods: The difference of compliance between direct observation method and concealed observation method was compared. The data were imported and exported using application program of sensor-controlled workplace. SPSS 26.0 was used for statistical analysis of the hand hygiene implementation rate of HCWs in the past one years. Results: The hand implementation rate in the concealed observation group was 81.8% from July 2020 to December 2020, and that of the direct observation group was 88.0% from January 2021 to June 2021, with statistical significance (P＜0.05). In the concealed observation group, the highest rate of hand hygiene was 90.3% for nurses, 74.3% for medical staff, 50.2% for medical technic staff, and 60.5% for other workers. In the direct observation group, the rate of hand hygiene rose to 93.6% for nurses and 84.0% for medical staff. The hand hygiene implementation rate of medical technic staff increased to 80.0%, and that of other staff increased to 87.0%, with statistical significance between the two groups (P＜0.05). The hand hygiene implementation rate of the direct observation group before contacting with patients, before aseptic operation and after contacting with surrounding objects was higher than that of the concealed observation group, and the difference was statistically significant (P＜0.05). Conclusion: The hand hygiene implementation rate of medical staff affected by Hawthorne effect under direct observation has been improved, but it is still necessary to further strengthen the hand hygiene management of medical staff, especially the personalized management of staff on different positions.

Objective: To evaluate clinical effect of full-time nursing team using fine nursing intervention in treating patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) combined with respiratory failure with non-invasive bilevel positive airway pressure (BiPAP) ventilator. Methods: A total of 82 cases of patients with AECOPD combined with respiratory failure treated with BiPAP ventilator in Dongtai People's Hospital of Jiangsu Province (April 2021 to April 2022) were selected and randomly divided into the control group (n=41) and observation group (n=41). The control group were given routine nursing intervention. The observation group was given fine nursing intervention by the full-time nursing team. The pulmonary function (forced expiratory volume in the first second, forced vital capacity, FEV1/FVC, ratio of FEV1 to predicted value), blood gas indexes (arterial partial pressure of carbon dioxide, arterial partial pressure of oxygen, arterial oxygen saturation, pH value) and complication rate of the two groups were compared. Results: Before nursing, the pulmonary function of the two groups was compared and no significant difference were found (P＞0.05). After nursing intervention, the pulmonary function of the observation group was significantly better than that of the control group (P＜0.05). There was no significant difference in blood gas index between the two groups before nursing (P＞0.05). However, the blood gas index of the observation group was better than that of the control group after nursing intervention(P＜0.05). Compared with the control group, the incidence of complications was lower in the observation group (P＞0.05). Conclusion: For AECOPD patients combined with respiratory failure, fine nursing intervention under full-time nursing team would be more likely to improve patients’ pulmonary function, blood gas indexes and reduce their incidence of complications.

Objective: To explore the effect of comprehensive nursing mode on negative emotions and quality of life of elderly patients with multiple diseases. Methods: From August 2022 to November 2022, 88 elderly patients with multiple diseases who were hospitalized in the Fifth People's Hospital of Sichuan Province were selected into this study. The patients were randomly divided into the observation group and control group, with 44 patients in each group. Both groups received treatment for common diseases. The control group received routine care, while the observation group received comprehensive care based on routine care. The self-care ability, negative emotions and quality of life of the two groups were compared. Results: The scores of self-care scale in the two groups after intervention were higher than those before intervention, and those in the observation group were higher than those in the control group (P＜0.05). The scores of SAS and BDI in the two groups after intervention were lower than those before intervention, and those in the observation group were lower than those in the control group (P＜0.05). After intervention, the SF-36 scores of patients in the two groups were both higher than those before the intervention, and the scores in the observation group were higher than those in the control group. The Newcastle Nursing Satisfaction Scale scores of the observation group was higher than those of the control group (P＜0.05). Conclusion: Compared with conventional nursing mode, comprehensive nursing mode can effectively improve the self-care ability of elderly patients with multiple diseases, alleviate negative emotions, and improve the quality of life and nursing satisfaction.