Objective: To study the expression level, prognosis and correlation with immune cell infiltration of BUB1B in pancreatic adenocarcinoma ( PAAD ) by online database and bioinformatics. Methods: The expression of BUB1B in various cancers was analyzed by GEPIA database. The correlation between the expression of BUB1B and the prognosis of PAAD patients was searched by GEPIA and Kaplan-Meier Plotter database. Then, the protein interaction network of BUB1B co-expressed genes was constructed by using String database, and its GO biological function and KEGG signaling pathway were analyzed by using David database. Finally, the TIMER database was used to analyze the correlation between the expression of BUB1B gene and immune cell infiltrating cells. Results: The expression level of BUB1B in PADD tissues was higher than that in normal tissues (P＜0.01). The high transcription level of BUB1B was closely related to gender, age, race, obesity, drinking habits, chronic pancreatitis, lymph node metastasis and cancer stage (P＜0.05). The expression level of BUB1B mRNA was associated with prognosis and immune cell infiltration (P＜0.05). Conclusion: BUB1B is highly expressed in PADD, which is related to prognosis and immune infiltration, and can be used as a potential marker for diagnosis, prognosis and drug treatment of pancreatic cancer.

Objective: To investigate the expression of miR-17-5p in lung adenocarcinoma cells, and to study the effects of miR-17-5p on proliferation, invasion, migration and apoptosis of lung adenocarcinoma cells and its molecular mechanism. Methods: The expression of miR-17-5p in human lung adenocarcinoma cell line HCC827 and human normal lung epithelial cell line BEAS-2B were detected by real-time fluorescent quantitative PCR. There were miR-17-5p mimics group, miR-17-5p inhibitor group and NC group in the experiment. The effects of miR-17-5p on proliferation, apoptosis, invasion and migration of lung adenocarcinoma cells were analyzed by CCK-8, flow cytometry and Transwell assay. Finally, PKD2 was predicted to be a potential downstream target gene of miR-17-5p by Targetscan database. The interaction between miR-17-5p and PKD2 was verified by real-time fluorescence quantitative PCR and Western-blot experiments. Results: miR-17-5p was highly expressed in HCC827 cells (P＜0.001), and up-regulation of miR-17-5p significantly promoted the proliferation (P＜0.05 ), invasion (P＜0.01 ) and migration (P＜0.05) of lung adenocarcinoma cells, and inhibited apoptosis (P＜0.01 ). There was a direct binding site between miR-17-5p and 3'UTR of PKD2, and overexpression of miR-17-5p significantly promoted the expression of PKD2 at both mRNA and protein levels (P＜0.01). Conclusion: PKD2 was the direct target gene of miR-17-5p, and miR-17-5p affected the biological behavior of lung adenocarcinoma cells by regulating PKD2.

Objective: To observe the effects of Muxiang Binglang Wan prescription on tumor necrosis factor α (TNF-α) and pepsinogen (PG) in mice, and to explore the prevention and treatment effect of Muxiang Binglang Wan prescription on alcohol-induced gastric injury in mice. Methods: A tota of 60 mice were randomly divided into 12 mice in blank control group (group A), 24 mice in model control group (group B) and 24 mice in Muxiang Binglang Wan intervention group (group C). Group A was given normal saline, group B was given 56° liquor and normal saline, and group C was given 56° liquor and Mu Xiang Binglang Wan, once every 24h for 1 week. The food intake, water intake and body weight of the mice were observed and recorded every day. At the end of the experiment, the concentrations of pepsinogen (PGⅠ、 PGⅡ) and tumor necrosis factor (TNF-α) in the blood of the mice were detected. Results: The mice in group A almost had no agitation, ate and drank as usual, moved flexibly, gained weight within the normal range, and had bright and glossy hair. The mice in group B were obviously restless and irritable, ate less, with the hair almost dull and grayish yellow, and the drained stool of them was found to be obvious flatulence and bleeding tendency in the stomach after death.The hair wetness of group C was better than that of group B. Compared with group B, the concentration of PGⅠ in groups A and C was significantly higher, the concentration of PGⅡ was significantly decreased (P＜0.05), and the concentration of TNF-α was significantly decreased (P＜0.05). Conclusion: Muxiang Binglang Wan has good effect on prevention and treatment of alcoholic gastric injury in mice.

Objective: To measure and analyze the anatomical parameters of the femur by measuring and analyzing the positive and lateral X-ray films of the full-length weight-bearing lower limbs and the three-dimensional model of the femur CT, and to clarify the correlation between the anatomical morphology of the femur and the sagittal force line, so as to provide help for the placement of knee prosthesis and the accurate recovery of the lower limb force line in total knee arthroplasty. Methods: Ten healthy volunteers were recruited. The full-length X-ray films of the lower limbs were taken to determine the coronal and sagittal force lines of the lower limbs and the weight-bearing ratio. The imaging data of 60 patients with full-length CTA of both lower limbs were collected. The CT data were reconstructed by Mimics software. The relevant anatomical parameters were measured by combining two-dimensional images and three-dimensional reconstruction models. The data were analyzed by K-S test. Results: (1)The standard weight-bearing positive bearing axis is located at 45% of the medial femoral condyle, and the lateral bearing axis is located at 44% before the transverse axis of the femoral condyle. (2) The femoral three-dimensional model measured femoral valgus angle, femoral lateral arch angle, femoral anterior arch angle, proximal femoral eccentricity, femoral neck anteversion, femoral neck shaft angle, and femoral length were 4.55°±1.31°, 0.41°±1.44°, 9.54°±1.57°, 37.04±4.74 mm, 14.99° ±7.52°, 131.17±7.23 °, and 414.24±23.70 mm, respectively; the angles between the femoral anatomical axis and the coronal and sagittal planes were 8.60±1.45, 0.75±0.60, 1.05±0.70 and 0.61±0.50, respectively; the angles between the femoral force line and the coronal and sagittal planes were 1.38°±0.93° and 4.77°±1.65°, respectively. (3)Correlation analysis showed that the femoral valgus angle was positively correlated with the femoral lateral arch angle (r=0.552, P＜0.001), the femoral valgus angle was positively correlated with the proximal femoral eccentricity (r=0.502, P＜0.001), and the femoral anterior arch angle was positively correlated with the femoral neck anteversion angle (r=0.543, P＜0.001); the angle between the femoral force line and the sagittal plane was positively correlated with the femoral anterior arch angle (r=0.823, P＜0.001), and the angle between the femoral force line and the sagittal plane was positively correlated with the femoral neck anteversion angle (r=0.424, P＜0.001). Conclusion: The angle between the femoral force line and the sagittal plane is closely related to the femoral anterior arch angle and the femoral neck anteversion angle. Therefore, if the femoral anterior arch angle and the femoral neck anteversion angle are larger before operation, the osteotomy angle should be adjusted accordingly, which is conducive to achieving a more reasonable alignment of the force line. The correlation between the anatomical morphology of the femur and the sagittal alignment of the femur described in this study has important reference significance for the placement of knee prosthesis and total knee arthroplasty.

Objective: Two dimensional speckle tracking imaging (2D-STI) combined with quantitative analysis system of right ventricle (QAS-R) was used to evaluate the changes of right ventricular function in patients with lymphoma after chemotherapy. Methods: Forty patients with lymphoma diagnosed in the hematology department and receiving anthracycline chemotherapy were selected. According to the chemotherapy cycle, they were divided into pre chemotherapy (T0), completion of 2 cycles (T1), completion of 4 cycles (T2) and completion of 6 cycles (T3).Left ventricular ejection fraction (LVEF), tricuspid annular systolic peak flow velocity(S’), tricuspid annular plane systolic excursion(TAPSE), FAC(fractional area change), early diastolic peak velocity of tricuspid annulus (E), late diastolic peak velocity of tricuspid annulus (A) were measured by conventional echocardiography, and E/A was calculated. Two dimensional speckle tracking imaging was used to measure strain of the base segment of the right ventricular free wall (Sb), strain of the middle segment of the right ventricular free wall (Sm), strain of the apex segment of the right ventricular free wall (Sa), and calculate longitudinal strain of the right ventricular free wall (RVFWLS).Right ventricular ejection fraction (RVEF) was measured by quantitative analysis system of right ventricle. The differences of the above parameters before and after chemotherapy were compared. Results: Compared with T0, LVEF and E/A had no significant difference in T1, T2 and T3 (P＞0.05); Compared with T0, RVFWLS, Sb, Sm and RVEF decreased significantly in T1, T2 and T3 (P＜0.05);Sa and RVFAC decreased significantly in T2 and T3 (P＜0.05);TAPSE and S’ decreased significantly in T3 (P＜0.05);Compared with T1, RVFWLS, Sb, Sm, Sa, RVEF and FAC decreased significantly in T2 and T3 (P＜0.05);TAPSE and S’ decreased significantly in T3 (P＜0.05);Compared with T2, RVFWLS, Sb, Sm, Sa, RVEF, TAPSE, FAC, S’ in T3 decreased significantly (P＜0.05). In the correlation analysis, the cumulative dose of anthracyclines was negatively correlated with RVFWLS, Sb, Sm, Sa, RVEF, RVFAC, TAPSE, S’, and the correlation coefficients were -0.775, -0.673, -0.628, -0.593, -0.749, -0.468, -0.368, -0.346, respectively;RVEF was positively correlated with RVFWLS, Sb, Sm, Sa, RVFAC, TAPSE, S’ and the correlation coefficients were 0.667, 0.597, 0.491, 0.544, 0.354, 0.258, 0.257, respectively. Conclusion: Two dimensional speckle tracking imaging combined with quantitative analysis system of right ventricle can evaluate the changes of right ventricular function in patients with lymphoma after chemotherapy.

Objective: To investigate the relationship between the proportion of CD19⁺CD27⁺IgD⁺B cells in peripheral blood and disease activity in patients with thyroid-associated ophthalmopathy (TAO). Methods: Flow cytometry was used to detect the proportion of CD19⁺CD27⁺IgD⁺B cells in TAO patients and healthy control groups. The difference in the proportion of CD19⁺CD27⁺IgD⁺B cells between TAO patients and healthy control groups was analyzed. The correlation between the proportion of CD19⁺CD27⁺IgD⁺B cells and activity was also analyzed. Results: The proportion of CD19⁺CD27⁺IgD⁺B cells in TAO patients was significantly lower than that in healthy controls (P＜0.05). The proportion of CD19⁺CD27⁺IgD⁺B cells in active TAO patients was significantly lower than in inactive TAO patients. The proportion of CD19⁺CD27⁺IgD⁺B cells was significantly negatively correlated with the CAS score of TAO patients (r=-0.7128, P＜0.001). Conclusion: The proportion of CD19⁺CD27⁺IgD⁺B cells in TAO patients is decreased, which is negatively correlated with disease activity.

Objective: To explore the application value of artificial intelligence (AI) in quantitative evaluation of CT images of different clinical types of COVID-19. Methods: A total of 169 patients with COVID-19 who underwent chest CT examination between October 2021 and June 2022 were included in the study, and were divided into a severe group of 14 cases and a non-severe group of 155 cases. The general data and imaging information of the COVID-19 patients were collected and analyzed retrospectively. The lesion volume fraction, GGO volume fraction, consolidation volume fraction, GGO + consolidation volume fraction and crazy-paving pattern volume fraction were measured and calculated with the assistance of the “AI qualitative auxiliary diagnosis system for CT images of COVID-19” provided by BIOMIND. Mann-whitney U test was used to compare the data of the two groups. The receiver operating characteristic curve (ROC) was used to evaluate the diagnostic efficacy of the above indexes in severe patients. Results: There were significant differences in lesion volume fraction and GGO volume fraction between the two groups (P＜0.05). However, there was no significant difference in the volume fraction of consolidation, GGO + consolidation and crazy-paving pattern (P＞0.05). The sensitivity of lesion volume fraction and GGO volume fraction for clinical classification of COVID-19 was 1 and 0.6, and the specificity was 0.822 and 0.923, respectively. Conclusion: The lesion volume fraction and GGO volume fraction calculated with the assistance of AI are sensitive indicators for clinical classification of COVID-19.

Objective: To investigate the predictive value of serum lipoprotein (a) [Lp(a)] and high-sensitivity C-reactive protein/high-density lipoprotein ratio (CHR) for short-term prognosis in patients with acute myocardial infarction (AMI). Methods: A total of 251 patients with AMI who underwent percutaneous coronary intervention in the Department of Cardiology of Baotou Central Hospital from July 2021 to July 2022 were selected for retrospective analysis. The general data, laboratory indexes, killip classification, ejection fraction (EF value) and coronary angiography results of the enrolled patients were collected. The CHR value and Gensini score were calculated according to the laboratory indexes and coronary angiography results. According to whether major adverse cardiovascular events (MACE) occurred during hospitalization of AMI patients, they were divided into observation group and control group. The differences of general data and laboratory indexes between the two groups were compared. Univariate and multivariate logistic regression were used to analyze the influencing factors of MACE in AMI patients during hospitalization. The receiver operating characteristic (ROC) curve was used to analyze the predictive value of Lp(a), CHR and Lp(a) combined detection of CHR for MACE. Results: The Lp(a), CHR, killip grade≥II and Gensini score in the observation group were higher than those in the control group, and the differences were statistically significant (P＜0.05). Lp (a) and CHR were independent risk factors for MACE in patients with AMI during hospitalization. ROC curve showed that the combined detection of Lp(a), CHR and Lp(a) CHR had predictive value for MACE in AMI patients during hospitalization, and the combined detection value was higher. Conclusion: Lp(a) and CHR are independent risk factors for MACE in AMI patients during hospitalization. Combined detection of Lp(a) and CHR is helpful to evaluate the short-term prognosis of AMI patients.

Objective: To investigate the prevalence and related factors of hyperuricemia among children and adolescents in Baotou, Inner Mongolia. Methods: The data of children and adolescents aged 3-18 years from January 2021 to June 2022 were collected. The eligible children and adolescents were taken as the research objects, and the data were sorted out and the results were statistically analyzed. Results: The difference of SUA level between different genders and age groups was statistically significant (P＜0.05); the detection rates of HUA in boys aged 3-6, 7-9 and 10-18 years old were 4.93%, 3.21% and 4.41%, respectively. The detection rates of HUA in girls in the above age groups were 4.44%, 3% and 2%, respectively. Among boys, there were significant differences in BUN, Cr, AST, Glu and blood lipid levels between HUA group and normal group (P＜0.05), and among girls, there were significant differences in AST levels between HUA group and normal group (P＜0.05). The results of correlation analysis showed that the SUA level in boys was positively correlated with BUN, Cr, ALT, TG and TC (P＜0.05), while that in girls was negatively correlated with Glu (P＜0.05), and positively correlated with BUN, Cr and ALT (P＜0.05). The results of multivariate logistic regression analysis showed that BUN and TC were the influencing factors of male HUA, and Glu was the influencing factor of female HUA. Conclusion: The serum uric acid level of children and adolescents in Baotou urban area is different in different ages and genders, and the detection rate of hyperuricemia in boys is higher than that in girls. Hyperuricemia can cause changes in BUN, Cr, AST, Glu and blood lipid levels; TC, BUN and Glu are the influencing factors of hyperuricemia in children and adolescents.

Objective: To investigate the correlation between the indexes of laboratory examination and the mortality of patients with osteoporotic vertebral compression fracture (OVCF) after percutaneous kyphoplasty (PKP). Methods: Patients who were diagnosed as OVCF and treated with PKP by the same surgical team in the Third Hospital of Baotou from June 2018 to June 2022 were selected. The blood routine, blood biochemical indexes and basic information of the patients at admission were collected, and the postoperative survival and Oswestry dysfunction index (ODI) questionnaire were followed up. According to whether the patients survived during the follow-up period, the patients were divided into survival group and death group. The related examination indexes of the two groups of patients at admission were compared, and the influencing factors of the death outcome of the patients were analyzed. Results: The 1-year mortality rate of 176 patients with OVCF was 6.81% (12/176), and the total mortality rate was 18.75% (33/176) during the follow-up period. The AUC of bone mineral density (BMD) in predicting death was 0.797 (95%CI: 0.699-0.895, P＜0.05), when the sensitivity was 66.7% and the specificity was 85.3%, the maximum diagnostic index was 0.520, and the diagnostic cut-off point was -3.430. The AUC of albumin (ALB) in predicting death was 0.676 (95%CI: 0.572-0.780, P＜0.05), when the sensitivity was 63.6% and the specificity was 70.6%, the diagnostic index was 0.342, and the diagnostic cut-off point was 39.450 g/L. The AUC of neutrophil-to-lymphocyte ratio (NLR) in predicting death was 0.786 (95%CI: 0.693-0.879, P＜0.05), when the sensitivity was 81.8% and the specificity was 65.0%, the diagnostic index was 0.468, and the diagnostic cut-off point was 3.120. The AUC of BMD, ALB and NLR combined to predict the death of patients was 0.871 (95%CI: 0.798-0.945, P＜0.05). When the sensitivity was 75.8% and the specificity was 86.7%, the maximum diagnostic index was 0.625, and the diagnostic cut-off point was 4.945, which was higher than the area under the curve of NLR, BMD and ALB. Conclusion: The combination of BMD, ALB and NLR (combined index Y=0.528×NLR-3.526×BMD-0.227×ALB) can better predict the mortality of patients.

Objective: To explore the diagnostic and prognostic value of FAM25A in pancreatic cancer by bioinformatics methods, analyze its correlation with immune infiltration, and elaborate the potential mechanism of FAM25A in the occurrence and development of pancreatic cancer. Methods: The Pancreatic cancer datasets were obtained from TCGA, GTEx and GEO databases, and Rstudio was used for data analysis and visualization. The expression difference of FAM25A was clarified. The relationship between the expression of FAM25A and the overall survival time of pancreatic cancer was analyzed by K-M. The correlation between FAM25 A expression and prognostic factors of pancreatic cancer was analyzed by Cox regression. The correlation between FAM25A expression and immune cell infiltration was analyzed. According to WGCNA, the module genes most related to FAM25A and tumor purity were imported into STRING to construct PPI network and obtain GO and KEGG. GSEA was used to analyze the enrichment pathway of high and low expression of FAM25A, and MOCDE clustering subnetwork in Cytoscape was used to obtain significantly related nodes. Based on the above information, the potential mechanism of FAM25A in pancreatic cancer was analyzed. Results: The results showed that FAM25A was significantly more highly expressed in pancreatic cancer (P＜0.01); K-M analysis showed that the survival time of patients with high expression of FAM25 A was significantly shorter than that of patients with low expression; Cox regression analysis of prognostic factors related to pancreatic cancer suggested that FAM25A was an independent risk factor for pancreatic cancer (HR=2.4, 95%CI=1.614-3.592); Correlation analysis of immune cell infiltration showed that FAM25A could reduce T lymphocyte level; GO, KEGG, GSEA and MCODE results showed that FAM25A mainly played a role in cell-cell interaction, intercellular signal generation and transduction, chemokine and immune response. Conclusion: FAM25A over-expression in pancreatic cancer is an independent poor prognostic factor in pancreatic cancer. Abnormal expression of FAM25A can also be used as bio-markers for diagnosis and prognosis of pancreatic cancer. Its biological effects may be achieved by promoting proliferation, invasion and metastasis of pancreatic cancer.

Objective: To investigate the contamination situation of the Salmonella Spp, Listeria monocytogenes and Campylobacter Spp in raw poultry and livestock meat in Inner Mongolia, so as to provide basic information for the prevention and control food-borne diseases, and guidance for relevant supervisory departments. Methods: A total of 3171 samples of raw poultry and livestock meat were collected from 12 cities from 2016 to 2020. According to the National Standard for Food Safety (GB 4789-2016, GB 4789-2014) and Manual for Food-borne Disease Surveillance, the pathogenic bacteria were monitored. Results: From 2016 to 2020, a total of 346 positive strains of pathogenic bacteria were detected in 3171 samples, with a total detection rate of 10.91% (346/3171).The total detection rates of Salmonella, Listeria monocytogenes and Campylobacter were 8.56% (85/993), 5.62% (50/889) and 16.37% (21/1289), respectively.The pathogen contamination rate of bulk samples is higher than that of pre-packed samples.The detection rate of Listeria monocytogenes in frozen meat was the highest, 17.53% (27/154).The detection rates of Salmonella were higher in online stores and convenience stores/retail stores (22.22% (4/18) and 14.57% (22/151), respectively.The highest detection rate of Campylobacter was 40.55% (103/254) in the breeding link, and no samples were detected in the slaughtering process.Conclusion:From 2016 to 2020 Campylobacter Spp contamination was the highest in raw poultry and livestock meat in Inner Mongolia, and Listeria monocytogenes was low. In order to reduce the incidence of foodborne diseases, it is necessary to strengthen the supervision and management of raw poultry and livestock meat in different segments, so as to ensure the food safety and health of the people.

Objective: To observe the application effect of "cluster + TCM emotion" nursing mode in patients with acute stroke. Methods: 100 patients diagnosed with acute stroke from January to December 2021 were selected as the observation group, receiving the nursing mode of "cluster + TCM emotion", and 100 patients who had previously received routine nursing of acute stroke in the emergency department were selected as the control group. The mini mental status examination (MMSE), activities of daily living (ADL) and nursing satisfaction before and after nursing intervention were evaluated. Results: The MMSE and ADL scores of the observation group were improved (P＜0.05), the improvement of the observation group was better than that of the control group (P＜0.05), and the nursing satisfaction of the observation group was higher than that of the control group (P＜0.05). Conclusion: The nursing mode of "cluster + TCM emotion" for patients with acute stroke can improve the quality of life of patients, improve nursing satisfaction, and is worth promoting.

Objective: To explore the effect of combined intervention on the treatment of patients with multi-drug resistant tuberculosis. Methods: A total of 200 MDR-TB patients were randomly divided into routine group and intervention group, with 100 patients in each group. The routine group received general health education and routine nursing. In the intervention group, the modified MoriskY medication compliance scale was used for questionnaire assessment and multinomial interventions such as group activities, moxibustion intervention, nutrition intervention and centralized management were implemented. Results: The total average score of medication compliance in the intervention group was 8.80±1.41 scores, which was higher than 8.21±1.63 score in the control group, and the difference was statistically significant (P＜0.05). The index values of five classification webs in the intervention group were significantly higher than those in the conventional group (P＜0.05). The conversion rate of sputum culture in the intervention group was significantly higher than that in the conventional group (P＜0.05). The CT cavity closure rate in the intervention group was significantly higher than that in the conventional group (P＜0.05). Conclusion: Combined intervention can effectively improve patients' medication compliance and treatment effect, and promote patients' recovery.

Abnormal DNA methylation is one of the important mechanisms of tumorigenesis and development. It is the core of establishing and maintaining tissue-specific gene expression, and also the mechanism of regulating gene expression, which plays an important role in tumorigenesis. In particular, the abnormal state of hypermethylation of tumor suppressor genes is of great significance in the occurrence, diagnosis, identification, prognosis and treatment of tumors. In addition, DNA methylation and demethylation are reversible processes. 5-Azacytidine can reduce the methylation level of hypermethylated tumor suppressor genes by inhibiting DNA methyltransferase, so as to achieve the goal of tumor treatment. In leukemia, myelodysplastic syndrome, precursor bone marrow cell tumors and solid tumors, 5-azacytidine inhibits cell proliferation and promotes cell differentiation through DNA demethylation, which has therapeutic potential. However, its low bioavailability and long-term use may cause DNA damage and cytotoxicity. Therefore, it is necessary to find better administration methods and dose regulation strategies to verify and optimize its anti-tumor efficacy and promote clinical application.

Schisandra chinensis is a kind of medicinal plant with important medicinal value and economic value in China. Endophytic fungi, endophytic bacteria and endophytic actinomycetes are the main components of endophyte of Schisandra chinensis. The endophyte of Schisandra chinensis can produce the same or similar active metabolites as the plants, such as lignans, triterpenes, organic acids and other active substances. With the continuous progress of scientific exploration, the role of endophytes in anti-bacterial, anti-cancer, anti-oxidation and biological control of pathogenic bacteria has been gradually discovered, which has become the focus of industry, medicine, agriculture and other related fields. In this paper, the research on the biological activity of endophytes and secondary metabolites of Schisandra chinensis at home and abroad in recent years is reviewed, which provides theoretical support for the development and application of secondary metabolites of endophytes of Schisandra chinensis.