Objective: To detect differentially expressed miRNAs in exosomes derived from intestinal epithelial cells of essential hypertensive rats, and to enrich and analyze their target genes. Methods: Three Spontaneously hypertensive rats (SHR) and three Wistar-Kyoto rats (WKY) were selected as the research objects. The expression of miRNA in exosomes derived from intestinal epithelial cells of rats was detected by high-throughput sequencing. The differentially expressed miRNAs in the two groups of rats were screened and their target genes were predicted. The target genes were analyzed by Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) using biological function software. Results: Compared with the WKY group, there were 37 differentially expressed miRNAs in the SHR group (All P＜0.05), 32 miRNAs were up-regulated and 5 miRNAs were down-regulated. Among them, rno-miR-208b-3p, rno-miR-134-5p, rno-miR-93-3p, rno-miR-378b, rno-miR-96-5p, rno-miR-210-3p, and rno-miR-208a-3p were noteworthy. A total of 10 662 miRNA downstream target genes were predicted, and a total of 34 127 target sites were predicted. GO enrichment was mainly concentrated in membrane-bound organelles, protein binding, and positively regulated biological processes. KEGG enrichment was mainly concentrated in MAPK, mTOR, Ras and TNF signaling pathways. Conclusion: The expression of miRNA in exosomes derived from intestinal epithelial cells of essential hypertensive rats was significantly different. Its target genes may be involved in MAPK, mTOR, Ras and TNF signaling pathways through cell growth and differentiation, angiogenesis and metabolic function, thus affecting the occurrence and development of hypertensive diseases.

Objective: To investigate the protective effect of Nox inhibitor APX-115 free base on H9c2 cells against high concentration of glucose. Methods: The proliferative activity of H9c2 cells after pretreatment with different concentrations of APX-115 free base was detected by CCK8 assay. The expression of Nox4 protein was detected by Western blot and immunofluorescence. The morphological changes of H9c2 cells were observed. Results: Compared with the Model group, whrn the concentration of APX-115 free base was 8 μmol/L, the cell proliferation activity decreased, and the cell proliferation activity increased at other concentrations. The expression of Nox4 protein in each treatment group of APX-115 free base was down-regulated. The cell morphology of the APX-115 free base 5 μmol/L treatment group returned to spindle shape, the cell distribution was gradually uniform, the intercellular space was reduced, and the number of cells basically returned to normal level. Conclusion: When APX-115 free base is 5 μmol/L, it has the best protective effect on H9c2 cells induced by high glucose.

Objective: To investigate the role of CCN1 protein in a proliferation-inducing ligand (APRIL) and B-cell activating factor (BAFF) in esophageal cancer. Methods: CCN1 was overexpressed in KYSE150 and OE-19 cell lines by plasmid transfection technique. The transfection effect was verified by Western blot, and the expression of APRIL and BAFF was detected. Results: APRIL/BAFF was highly expressed after overexpression of CCN1 in KYSE150 cells (P＜0.05), and APRIL/BAFF was lowly expressed after overexpression of CCN1 in OE19 cells (P＜0.05). Conclusion: CCN1 activates APRIL/BAFF in esophageal squamous cell carcinoma cell line KYSE150 and inhibits APRIL/BAFF in esophageal adenocarcinoma cell line OE19.

Objective: To study the expression levels of 14-3-3β and miRNA-152 in primary hepatocellular carcinoma (HCC), and to clarify their relationship with clinicopathological features and the relationship between 14-3-3β and miRNA-152. Methods: Forty patients of HCC tissues and adjacent tissues admitted to Baotou Cancer Hospital from 2019 to 2023 were selected. The expression levels of 14-3-3β mRNA and miRNA-152 were detected by RT-qPCR. The expression of 14-3-3β protein was detected by immunohistochemical PS method and Western blot, and the differences in expression levels were analyzed. Results: The expression of 14-3-3β in HCC tissues was higher than that in adjacent normal tissues, and the expression of miRNA-152 in HCC tissues was lower than that in adjacent normal tissues, meanwhile, the expression of 14-3-3β was relatively low when the expression of miRNA-152 was relatively high (P＜0.05). Moreover, the expression of 14-3-3β and miRNA-152 was negatively correlated in HCC (P＜0.05). Conclusion: The expression of 14-3-3β protein is up-regulated in HCC tissues, and the expression of miRNA-152 is down-regulated in HCC tissues. In HCC, miRNA-152 can reduce the expression of 14-3-3β through negative regulation mechanism.

Objective: To investigate the protective mechanism of TAK-242 on hippocampal inflammatory injury in rats with post-stroke depression. Methods: A total of 54 male 3-month-old SD rats were divided into sham operation group (n=18), post-stroke depression (PSD) model group (n=18) and TAK group (n=18). The middle cerebral artery occlusion (MCAO) model was established by suture method, and then chronic unpredictable mild stress (CUMS) stimulation was performed for 3 weeks to establish the PSD model. After the PSD model was successfully established, the TAK group was intraperitoneally injected with diluted TAK-242 0.3 mg/kg, which was performed on the 5th, 10th, 15th and 20th day of CUMS, respectively. The level of IL-1β in peripheral blood was detected by enzyme-linked immunosorbent assay. Results: Compared with the sham operation group, the body weight, sucrose preference index and movement (LA) of the PSD group and the TAK group were lower than those of the sham operation group, and the level of IL-1β was significantly increased (P＜0.05). Compared with PSD group, the body weight, sucrose preference index and LA of rats in TAK group were increased, and the expression level of IL-1β was decreased (P＜0.05). Conclusion: TAK-242 can reduce the expression of IL-1β in peripheral blood by inhibiting TLR4 signaling pathway, thus exerting a neuroprotective effect on hippocampal inflammatory injury in rats with post-stroke depression, and providing new ideas for clinical prevention and treatment of post-stroke depression.

Objective: To analyze the core drug pairs and their molecular biological mechanisms for the treatment of hepatitis B cirrhosis based on data mining and network pharmacology. Methods: Retrieve all the literatures related to the application of traditional Chinese medicine in the treatment of hepatitis B cirrhosis from January 2000 to December 2022 in China National Knowledge Infrastructure, Wanfang Database and VIP Science and Technology Journal, and study the medication rules of the prescriptions that meet the standards. The commonly used core drug pairs were subjected to pharmacological analysis, and the active components, main targets, and action pathways were predicted. Finally, molecular docking technology was used to simulate and verify on the computer. Results: A total of 275 prescriptions for the treatment of hepatitis B cirrhosis were screened. The analysis showed that TCM for the treatment of hepatitis B cirrhosis included 13 kinds of drugs such as Huangqi and Danshen. The core combination mode was 28 groups. Quercetin,Luteolin,Kaempferoland other ingredients were found as the main components of core prescription drugs for the treatment of hepatitis B cirrhosis,involving core targets such as serine/threonine protein kinase 1 (AKT1), tumor necrosis factor (TNF), tumor protein p53 (TP53), interleukin-6 (IL-6), vascular endothelial growth factor A (VEGFA), Caspase-3 (CASP3) and phosphatidylinositol-3-kinase/protein kinase (PI3K/Akt), TNF, interleukin-17 (Interleukin-17). Conclusion: The treatment principle of hepatitis B cirrhosis is to strengthen the body and relieve masses. "Huangqi-Danshen" can play a role in regulating cell growth, proliferation, survival and inflammatory reaction by interfering with PI3K-Akt, TNF, IL-17 and other signal pathways, so as to delay the progression of liver cirrhosis in hepatitis B.

Objective: To investigate the metabolic changes and possible metabolic pathways of hypertension with or without left ventricular hypertrophy (LVH) and healthy people. Methods: Thirty patients with hypertension (HTN) (HTN group), 30 patients with hypertension and LVH (LVH group) and 30 healthy volunteers (control group) were recruited. The metabolic profiles of plasma samples were obtained by non-targeted metabolomics. Results: A total of 1141 metabolites were detected, of which 43 were significantly different only in LVH group and control group, such as leucine, adenine, magnolol and so on. Leucine and adenine were up-regulated in LVH patients. According to the analysis of KEGG pathway, a total of 22 differential metabolites were involved in the metabolic pathway of LVH. Compared with HTN group, linoleic acid metabolism and α-linolenic acid (α-linolenicacid,ALA) metabolic pathway were down-regulated in LVH group. Conclusion: Compared with healthy subjects, the metabolic spectrum of patients with LVH is significantly different, indicating that myocardial fatty acids may be protective factors of LVH, leucine and adenine may be risk factors of LVH, and LVH can be improved by α-linolenic acid metabolic pathway.

Objective: To investigate the difference between the same isocenter plan and dual-center plan when using cone-beam CT (CBCT) for breast cancer breast-conserving surgery with chest wall and supraclavicular fields combined radiotherapy for reference of clinical treatment. Methods: Ten patients with the same isocenter and 10 patients with double isocenters were collected after breast-conserving surgery with three-dimensional conformal radiation therapy (3D-CRT) irradiation of the chest wall and supraclavicular field, and the patients applied CBCT to record the positional error data of X (left and right), Y (head and foot), and Z (front and back) in the three linear directions of the center points (once a week) to keep the same conditions of the original plan, and the centers were corrected using the positional error to generate error plans. The centroid coordinates were corrected using the positioning errors and brought into the planning system to generate an error plan. Under the premise that 90% of the target volume of the original plan reached 90% of the prescribed dose, the changes in the actual dosimetric parameters of the planning target volume (PTV) and organs at risk and the differences in the therapeutic parameters of the two types of single-center and dual-center radiotherapy plans caused by the errors were evaluated. Results: Compared with single-center and double-center error plans, the maximum dose Dmax (t=-4.744, P＜ 0.001), mean dose Dmean (t=-2.288, P=0.025), percentage of volume encompassed by 90% of the prescribed dose V90% (t=-3.216, P=0.002), and 110% of the volume encompassed by 110% of the prescribed dose (Z=-3.838, P＜0.001), and hotspot dose D1cc (t=-6.051, P＜0.001) were lower in the single-center group than those in the double -center group, and the difference was statistically significant (P＜0.05). In terms of organs at risk, the percentage of volume encompassed by 500 cGy dose in the contralateral lung, V5 (Z=-2.373, P=0.018), Dmean (Z=-2.534, P=0.011), spinal cord Dmax (=-4.417, P＜0.001), and thyroid Dmean (t=-4.535, P＜0.001) were higher in the two-center group than those in the single-center group, the difference was statistically significant (P＜0.05), and the differences in the remaining parameters were not statistically significant. In terms of treatment parameters, the treatment time (t=-2.621, P=0.010) was higher in the two-center group than in the single-center group, and the difference was statistically significant (P＜0.05), while the difference in the parameters of the number of machine jumps was not statistically significant. Conclusion: The same isocenter radiotherapy plan of chest wall field combined with supraclavicular field after breast conserving surgery for breast cancer is superior to the double isocenter radiotherapy plan in reducing the error leading to high dose in the target area, dose hotspots and protection of organs at risk.

Objective: To investigate the effects of intravenous general anesthesia combined with epidural block and ultrasound-guided transversus abdominis plane block on postoperative analgesia and early rehabilitation in patients undergoing laparoscopic ovarian cystectomy. Methods: One hundred patients undergoing laparoscopic ovarian cystectomy, ASA grade I or II, were randomly divided into transversus abdominis plane block analgesia group (group A, n=50) and epidural block analgesia group (group B, n=50). The operation time of block analgesia, the residence time in the anesthesia recovery room, the VAS scores before and 2 h, 4 h, 8 h, 12 h and 24 h after operation, the use of rescue analgesics, the recovery time of diet after operation, the time of first getting out of bed after operation, the occurrence of headache, vomiting, nausea and hypotension after operation, and the patient satisfaction were compared between the two groups. Results: The operation time of block analgesia in group A was shorter than that in group B (P＜0.05). There was no significant difference in residence time in anesthesia recovery room between the two groups (P＞0.05). The VAS scores of group B at 2 h, 8 h, 12 h and 24 h after operation were lower than those of group A (P＜0.05). The usage rate of rescue analgesics in group B was lower than that in group A (P＜0.05). There was no significant difference in postoperative diet recovery time and the first time to get out of bed after operation between the two groups (P＞0.05). Except for hypotension, there was no significant difference in the incidence of postoperative headache, nausea and vomiting between the two groups (P＞0.05). The patient satisfaction of group B was higher than that of group A (P＜0.05). Conclusion: For patients undergoing laparoscopic ovarian cystectomy under general anesthesia, the analgesic effect and analgesic time of combined epidural block are better than those of combined transversus abdominis plane block ; however, both have little effect on early postoperative rehabilitation.

Objective: To investigate the clinical value of ureteral occlusion apparatus for reverse blocking in percutaneous nephrolithotomy (PCNL). Methods: Sixty patients with renal calculi hospitalized in the Department of Urology, the First Affiliated Hospital of Bengbu Medical College from September 2019 to September 2022 were selected. Among them, 30 patients who used ureteral occlusion apparatus for reverse blocking during PCNL were used as the observation group, and 30 patients who were treated with conventional PCNL were used as the control group. The amount of bleeding, operation time, stone clearance rate, downward rate and incidence of related complications were compared between the two groups, and the efficacy was evaluated comprehensively by excellent, good and poor grades. Results: There was no significant difference in intraoperative bleeding volume between the observation group [(55.83±2.07)mL] and the control group [(55.73±2.02)mL] (P＞0.05). The operation time and stone clearance rate of the observation group were 48.50(47.00-50.00)min and 96.67%, which were higher than 46.00 (45.00-48.00) min and 80.00% of the control group (P＜0.05). The stone moving rate of the observation group was 3.33%, and the incidence of related complications was 3.33%, which were lower than 23.33% and 23.33% of the control group (P＜0.05). Conclusion: The application of ureteral occlusion apparatus in PCNL for reverse blocking can effectively promote stone clearance and avoid adverse events such as stone downward movement.

Objective: To investigate the effect of biological prosthesis hip replacement on hip function and serum inflammation in elderly patients with femoral neck fracture. Methods: A total of 40 elderly patients with femoral neck fracture admitted to the First Affiliated Hospital of Baotou Medical College were selected as the study subjects. The Harris scores before and after hip replacement with biological prosthesis were compared. The incidence of complications after treatment and the changes of serum inflammatory factors interleukin-6 (IL-6) and interleukin-10 (IL-10) before and 3 and 7 days after operation were compared. Results: The Harris score of the patients after 3 months of surgical treatment was significantly higher than that before surgical treatment (P＜0.05). There were 1 case of prosthesis dislocation and 1 case of periprosthetic fracture after operation. The incidence of complications was 5.0% (2/40). Compared with the patients before operation, the serum IL-6 levels at 3 and 7 days after operation showed a significant decrease (P＜0.05), and the serum IL-10 levels showed a significant increase (P＜0.001). Conclusion: For elderly patients with femoral neck fracture, biological prosthesis for hip replacement surgery has fewer complications, and can improve hip function, reduce inflammatory response, and has clinical promotion value.

Objective: To investigate the risk factors associated with the prognosis of pulmonary hypertension associated with left heart disease (PH-LHD). Methods: From September 2020 to February 2021,132 patients with PH-LHD who met the inclusion and exclusion criteria in the Department of Cardiology, the First Affiliated Hospital of Baotou Medical College and the Second Affiliated Hospital of Baotou Medical College were included and followed up for an average of 12 months. Demographic data, biochemical indicators, echocardiographic parameters, and clinical events of all patients were collected. The main event was all-cause mortality. Univariate and multivariate COX proportional hazard regression models were used to screen the risk factors related to the prognosis of patients with left heart-related pulmonary hypertension. The relationship between PASP and the prognosis of patients with pulmonary hypertension was analyzed by Kaplan-Meier survival curve. Results: A total of 132 patients with PH-LHD were included, of which 109 (82.6%) patients completed the follow-up, with an average follow-up of 12 (8.1, 15.9) months, and a total of 31 patients died. Univariate COX regression analysis showed that age, pulmonary artery systolic pressure (PASP), left ventricular end-diastolic diameter (LVDD), right ventricular diameter (RVD), pulmonary artery flow rate, hemoglobin, high-sensitivity C-reactive protein (hs-CRP), aspartate aminotransferase (AST), blood glucose (GLU), PT, INR, triglyceride (TG), and free thyroxine (FT4) were risk factors for prognosis in patients with PH-LHD (all P＜0.05). Multivariate COX regression analysis showed that age, LVDD, RVD, Hb, hs-CRP, PASP (P＜0.01) and GLU (P＜0.05) were independent risk factors for the prognosis of PH-LHD patients. Kaplan-Meier survival curve showed that the incidence of all-cause death in patients with PASP >52 mmHg was higher than that in patients with PASP ≤52 mmHg (P＜0.01). Conclusion: Age, LVDD, RVD, Hb, hs-CRP, GLU and PASP are independent risk factors for all-cause mortality in patients with pulmonary hypertension associated with left heart disease. The incidence of all-cause mortality in patients with PASP >52 mmHg is higher than that in patients with PASP ≤52 mmHg.

Objective: To analyze the effect of cone-beam computed tomography (CBCT) guided radiotherapy for malignant tumors. Methods: A total of 68 patients with malignant tumors who received radiotherapy from October 2021 to October 2022 were selected as the research objects. They were randomly divided into observation group and control group. Among them, 34 patients in the observation group received CBCT-guided radiotherapy, with a frequency of 1 time/d. 34 patients in the control group also received CBCT-guided radiotherapy, with a frequency of 1 time/3 d. The clinical effects of CBCT-guided methods were compared and analyzed. Results: The absolute values of x, y, z axis setup errors and setup expansion boundary in the observation group were lower than those in the control group (P＜0.05). The maximum irradiation dose and average irradiation dose of malignant tumor patients in the gross tumor area and planned target area in the observation group were higher than those in the control group (P＜0.05). The objective effective rate of the observation group was higher than that of the control group (P＜0.05). The quality of life scores in all fields of the observation group were higher than those of the control group (P＜0.05). Conclusion: For patients with malignant tumors, CBCT scanning and guidance before treatment can reduce the positioning error during radiotherapy. With the increase of CBCT scanning frequency, the adaptability and accuracy of radiotherapy can be better improved, the treatment effect can be ensured, and the discomfort reaction can be prevented.

Objective: To investigate the correlation between obstructive sleep apnea (OSA) and lung function and cognitive impairment, and to investigate the possible mechanisms of lung function decline and cognitive impairment caused by OSA. Methods: Forty-six patients with obstructive sleep apnea hypopnea syndrome (OSAHS) diagnosed by polysomnography (PSG) from January 2021 to June 2022 were collected. According to the apnea hypopnea index (AHI), they were divided into mild to moderate group (n=20) and severe group (n=26). Healthy subjects (n=23) were selected as the control group. The general data, PSG, lung function, blood gas analysis, and Montreal Cognitive Assessment Scale (MoCA) scores of the subjects were compared and statistically analyzed. Results: The vital capacity (VC), forced expiratory volume in 1 second percent predicted (FEV1%pred), forced expiratory volume in one second/forced vital capacity (FEV1/FVC%), expiratory reserve volume (ERV), functional residual capacity (FRC), maximum mid-expiratory flow rate (MMEF%) and 50% forced expiratory flow rate (FEF50%) in the severe group were lower than those in the mild-to-moderate group and the healthy group (P＜0.05). The partial pressure of oxygen (PaO₂) in the severe group was lower than that in the mild to moderate group and the healthy group (P＜0.05). There was significant difference in MoCA score among the three groups (P＜0.05). Correlation analysis showed that PaO₂ was negatively correlated with AHI and the longest apnea time, and positively correlated with LSaO₂. Conclusion: The lung function and cognitive ability of OSA patients are impaired, and the lung function and cognitive ability of patients with severe OSA are more obvious. Night hypoxia and low daytime PaO₂ play an important role in the mechanism of lung function decline and cognitive impairment in OSA patients.

Objective: To analyze the relationship between neutrophil/lymphocyte ratio (NLR) and postoperative recurrence and metastasis in patients with advanced gastric cancer after radical gastrectomy. Methods: A total of 96 patients with advanced gastric cancer who underwent radical gastrectomy in the hospital from December 2016 to February 2019 were selected as the case group, and 65 healthy volunteers who came to the hospital for physical examination during the same period were selected as the healthy control group. The NLR levels of the two groups were compared. After 3 years of follow-up, the patients were divided into recurrence and metastasis group and non-recurrence and metastasis group according to whether there was recurrence and metastasis after operation at the last follow-up. The NLR level and related factors that may affect postoperative recurrence and metastasis were compared between the two groups. Logistic regression analysis was used to analyze the risk factors of postoperative recurrence and metastasis in patients with advanced gastric cancer. The receiver operating characteristic curve (ROC) was drawn to analyze the predictive value ofNLR for postoperative recurrence and metastasis in patients with advanced gastric cancer after radical gastrectomy. Results: The level of NLR in the case group was significantly different from that in the healthy control group (P＜0.05). Univariate analysis showed that TNM stage, tumor differentiation, depth of invasion, postoperative radiotherapy and chemotherapy, tumor diameter and NLR were significantly different between the two groups (P＜0.05). Multivariate analysis showed that TNM stage, depth of invasion, tumor diameter and NLR were the influencing factors of postoperative recurrence and metastasis in patients with advanced gastric cancer after radical gastrectomy (P＜0.05). ROC curve showed that the area under the curve of NLR in predicting postoperative recurrence and metastasis in patients with advanced gastric cancer after radical gastrectomy was 0.732. Conclusion: NLR is highly expressed in patients with advanced gastric cancer after radical gastrectomy. As a factor affecting postoperative recurrence and metastasis, NLR has certain clinical value in predicting postoperative recurrence and metastasis.

Objective: To analyze the expression and correlation of human papillomavirus type 16 E6 protein (HPV16-E6), inhibitor of intracellular differentiation-1 (ID-1) and prolylcis-trans isomerase1 (Pin1) in cervical cancer. Methods: A total of 65 patients with cervical cancer admitted to the Affiliated Hospital of Putian University from March 2017 to March 2022 were selected. All patients underwent surgical resection of cancer tissues in our hospital, and cervical cancer tissues and adjacent tissues more than 2 cm from the edge of cancer tissues were retained. The positive expression rates of HPV16-E6, ID-1 and Pin1 in cancer tissues and adjacent tissues were detected by immunohistochemistry. The relationship between HPV16-E6, ID-1, Pin1 and clinical features was analyzed. Results: Compared with adjacent tissues, the positive rates of HPV16-E6, ID-1 and Pin1 in cancer tissues were higher (P＜0.05). HPV16-E6, ID-1 and Pin1 were positively correlated with depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter (P＜0.05).. After controlling for age, pathological type and other factors, HPV16-E6, ID-1 and Pin1 were correlated with depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter (P＜0.05). Conclusion: HPV16-E6, ID-1 and Pin1 are highly expressed in patients with cervical cancer, and HPV16-E6, ID-1 and Pin1 are related to the depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter in patients with cervical cancer.

Objective: To investigate the diagnostic value of human cytomegalovirus (HCMV) -deoxyribonucleic acid (DNA) quantitative detection in children with HCMV infection. Methods: A total of 2 256 children with suspected HCMV infection admitted to our hospital from January 2020 to December 2022 were selected. All suspected children were collected morning urine, alveolar lavage fluid, anticoagulant and procoagulant. After the completion of sample processing, HCMV-DNA was detected by fluorescence quantitative PCR and serum HCMV-IgM was detected by multiple microsphere flow immunofluorescence. Then the positive rates of HCMV-DNA among different types of specimens, different genders, different ages and the two detection methods were compared. Results: The positive rate of HCMV-DNA in urine was higher than that in alveolar lavage fluid and blood samples, and the positive rate of HCMV-DNA in alveolar lavage fluid samples was higher than that in blood samples, the difference was statistically significant (P＜0.05). There was no significant difference in the positive rate of HCMV-DNA in urine, alveolar lavage fluid and blood between male and female children (P＞0.05). The positive rate of HCMV-DNA in alveolar lavage fluid samples of children aged ≤28 d was higher than that of children aged 29 d to 1 year old and above 1 year old, and the positive rate of HCMV-DNA in urine samples of children aged 29 d to 1 year old was higher than that of children aged≤28 d and above 1 year old, the difference was statistically significant (P＜0.05). There was no significant difference in the positive rate of HCMV-DNA in blood samples of children aged≤28 d, 29 d to 1 year old and over 1 year old (P＞0.05). The positive rate of HCMV infection by HCMV-DNA was higher than that by HCMV-IgM detection, and the difference was statistically significant (P＜0.05). Conclusion: The detection rate of HCMV-DNA in urine samples is the highest, which is of great value for the diagnosis of HCMV infection in children, and the collection of urine samples is more convenient, especially for children with small months of age, and worthy of wide application.