Objective: To investigate the relationship between the activity and gene polymorphism of coagulation factor Ⅻ and the risk of recurrent spontaneous abortion (RSA) in Baotou Han population. Methods: RSA patients and healthy pregnant women were selected as the research objects. The activity of coagulation factor Ⅻ was measured. The genotype composition of FⅫ gene single nucleotide polymorphism (SNP) rs1801020 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results of the two groups were analyzed to determine the correlation between FⅫ gene SNP rs1801020 and RSA under different genetic models. Results: There were significant differences in the genotype and allele frequency distribution of SNP rs1801020 in FⅫ gene between the two groups (P＜0.05); the frequency of T allele in RSA group was lower than that in control group (χ²=8.756, OR=2.043, 95%CI: 1.267-3.295,P<0.05); the frequency of CT genotype in RSA group was higher than that in control group, while the frequency of TT genotype was lower than that in control group, and the difference was statistically significant (χ²=9.905, OR=2.611, 95%CI: 1.447-4.713,P＜0.05); FⅫ activity in RSA patients was different among rs1801020 genotypes, and the order of activity was CC, CT and TT. FⅫ SNP rs1801020 was associated with the risk of RSA under codominant, dominant and overdominant models (OR=2.611, 2.636, 2.470, P＜0.05); compared with TT genotype, CT and CT+CC genotypes could increase the risk of RSA; compared with CC+TT genotype, CT genotype could increase the risk of RSA. Conclusion: The difference in the distribution of FⅫ rs1801020 between the two groups may be related to the risk of RSA in Baotou Han women. Compared with TT genotype, mutant heterozygous CT genotype may increase the risk of RSA, the T allele of rs1801020 locus of FⅫ gene can cause the decrease of FⅫ activity.

Objective: To study and analyze the changes of intestinal flora in 1-5 years after cholecystectomy. Methods: A total of 15 fecal samples of healthy people and 16 fecal samples of people 1 to 5 years after cholecystectomy were collected from Baotou Central Hospital. The collected samples were sequenced to analyze the composition, richness and diversity of intestinal microbial flora in the two groups, and to compare the differences of intestinal microbial flora between the two groups. Results: (1) At the phylum level, the relative abundance of Bacteroidetes, Proteobacteria, Verrucomicrobia and Fusobacteria increased, while the relative abundance of Actinobacteria decreased in the population 1-5 years after cholecystectomy. (2) At the genus level, the relative abundance of Escherichia, Ruminococcus, Blautia, and Gemmatiaceae increased, while the relative abundance of Faecalibacterium, Bifidobacterium, Collins, and Agathobacter decreased in the population 1 to 5 years after cholecystectomy. The Chao1 index and Observed _ species index of the healthy population group were higher than those of the population group 1-5 years after cholecystectomy, and the differences were statistically significant (P＜0.05). The Pielou evenness of the healthy population group was slightly lower than that of the population group 1-5 years after cholecystectomy, and the difference was statistically significant (P＜0.05). Lachnospiraceae, Ruminococcus, Faecalimonas and Burkholderia were the intestinal flora with significant differences between the groups in the population group from 1 to 5 years after cholecystectomy. Conclusion: (1) The composition of intestinal flora in the population 1-5 years after cholecystectomy has changed. (2) The relative abundance of short-chain fatty acid-producing bacteria and probiotics in the population 1-5 years after cholecystectomy decreased, while the relative abundance of pathogenic bacteria increased.

Objective: To analyze the clinicopathological features and laboratory characteristics of patients with dyslipidemia and liver dysfunction. Methods: The clinical data of 135 patients with liver dysfunction who underwent physical examination in the hospital from May 2019 to March 2022 were retrospectively analyzed. And they were divided into dyslipidemia group (n=60) and non-dyslipidemia group (n=75) according to whether accompanied by dyslipidemia. All patients underwent B-ultrasound examination, and the clinical data, clinicopathological characteristics, liver function indexes, lipid metabolism indexes and inflammatory indexes of were compared between the two groups. Results: There was no significant difference in gender and age between the two groups (P＞0.05). The proportion of liver and abdominal pain, the proportion of ascites and the incidence of total complications in the dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). The proportion of liver capsule thickening, echo enhancement, liver density decrease and liver margin roughness in dyslipidemia group was higher than that in non-dyslipidemia group (P＜0.05). The levels of total bilirubin (TBIL), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). The level of high density lipoprotein cholesterol (HDL-C) in dyslipidemia group was lower than that in non-dyslipidemia group (P＜0.05), and the levels of triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). The levels of serum C-reactive protein (CRP) and interleukin-6 (IL-6) in dyslipidemia group were higher than those in non-dyslipidemia group (P＜0.05). Conclusion: The clinicopathological features of patients with liver dysfunction and dyslipidemia are mainly characterized by liver dysfunction, dyslipidemia, liver ascites, abdominal pain, high incidence of complications, decreased liver density, enhanced echo, rough liver margin, and increased inflammatory response.

Objective: To analyze the related indexes of vaginal microecology that affect the infection and outcome of high-risk human papillomavirus (HR-HPV), and to explore the correlation between HR-HPV and vaginal microecology, so as to provide the corresponding basis for the diagnosis and treatment of HR-HPV infection. Methods: Retrospective analysis of patients who underwent HR-HPV and vaginal secretion tests at the First Affiliated Hospital of Baotou Medical College from September 2021 to March 2023. According to the inclusion criteria, 1 052 cases of HR-HPV infection were selected, while 526 cases in the positive group and 526 cases in the negative group, and 131 cases in the returned portion: 76 cases in the returned group and 55 cases in the persistently infected group. The differences in the vaginal microecology of the above patients were compared between the groups. Results: (1) Age was correlated with HR-HPV infection and regression (P＜0.05). (2)The proportion of abnormal vaginal cleanliness, bacterial vaginitis (BV), pH＞4.5, H₂O₂(+),leukocyte esterase (LE) (+),and sialidase (SNA) (+)in the HR-HPV positive group was higher than that in HR-HPV negative group (P＜0.05); abnormal cleanliness, and SNA(+) were the independent risk factors for HR-HPV infection (P＜0.05). (3)The proportion of abnormal flora density, abnormal diversity, abnormal cleanliness, BV, pH＞4.5, H₂O₂(+), LE(+), SNA(+) in persistently infected group was higher than that of the regression group (P＜0.05); abnormal density, pH＞4.5, H₂O₂(+), LE(+) were the independent risk factors for the persistently infected of HR-HPV (P＜0.05); there was no change in the comparison of vaginal cleanliness before and after the regression of HR-HPV (P＞0.05). Conclusion: Vaginal microecological imbalance is associated with HR-HPV infection and persistence. Bacterial vaginitis, elevated pH value and abnormal H₂O₂ are risk factors for HR-HPV infection and persistence.

Objective: To investigate the mechanism of Banxia Baizhu Tianma Decoction (BBTD) on ischemic stroke. Methods: A total of 90 SD rats (245-280 g) were selected to prepare MCAO animal model by referring to Zea-longa's modified suture method. The cerebral infarction area of MCAO model animals was detected by TTC staining. The protein expression level of IL-6 in ischemic penumbra of ischemic brain tissue was detected by WB method, and the mRNA expression level of IL-6 in ischemic penumbra of ischemic brain tissue was detected by RT-PCR method. Results: Compared with the sham operation group, the percentage of cerebral infarction area in the model group increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the percentage of cerebral infarction area in BBTD and nimodipine treatment groups decreased to varying degrees, and the difference was statistically significant (P＜0.05). Compared with the sham operation group, the protein expression level of inflammatory factor IL-6 in the model group was significantly increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the protein expression level of inflammatory factor IL-6 in the BBTD and nimodipine treatment groups was decreased to varying degrees, and the difference was statistically significant (P＜0.05). Compared with the sham operation group, the mRNA expression level of inflammatory factor IL-6 in the model group was significantly increased, and the difference was statistically significant (P＜0.05). Compared with the model group, the mRNA expression level of inflammatory factor IL-6 in BBTD and nimodipine treatment groups was decreased to varying degrees, and the difference was statistically significant (P＜0.05). Conclusion: Classic Banxia Baizhu Tianma Decoction can effectively alleviate ischemic brain injury in stroke events, which may be related to the inflammatory response mediated by IL-6.

Objective: To explore whether triptolide can alleviate the disease by reducing the infiltration of neutrophils in the joint cavity of collagen-induced arthritis (CIA) mice, and to analyze whether triptolide can reduce the formation of neutrophil extracellular capture networks (NETs) in patients with rheumatoid arthritis (RA) and its mechanism of action, so as to provide theoretical support for the clinical application of tripterygium glycosides in RA. Methods: The core targets of Tripterygium wilfordii intervention in RA disease were explored by using the public database of network medicine. After the CIA model was constructed using DBA1 mice, triptolide was given for 60 days. The hind limbs of the mice were collected for HE staining and the degree of inflammatory cell infiltration in the joint cavity of the mice was observed. Neutrophils in peripheral blood of RA patients were collected and stimulated with triptolide for 2 h. The formation of NETs was observed by immunofluorescence staining. Treatment with triptolide in RA group and triptolide could alleviate joint swelling in CIA mice; triptolide could also inhibit the formation of NETs in peripheral blood neutrophils of RA patients. Transcriptome sequencing was performed on neutrophils in RA group and Tripterygium wilfordii treatment group. Results: Through network pharmacology analysis, PPI protein interaction was performed on the locked overlapping genes, and KEGG and GO analysis were performed. It was found that IL-17, TNF signaling pathway and apoptosis-related pathway were closely related to RA disease. In vivo experiments showed that 389 differential genes were screened from the transcriptome sequencing results of the triptolide treatment group. The GO and KEGG pathways were mainly enriched in JAK-STAT, TNF, and PI3K-Akt signaling pathways. Conclusion: Triptolide can alleviate joint swelling in CIA mice by down-regulating neutrophil infiltration in the joint cavity of CIA mice, and triptolide can reduce the formation of NETs in peripheral blood of RA.This phenomenon may be related to JAK-STAT signaling pathway, TNF signaling pathway, PI3K-Akt and other signaling pathways.

Objective: To investigate the effect of berberine (BBR),a traditional Chinese medicine extract,on doxorubicin (DOX) induced myocardial cell damage at the cellular level,and explore the possible mechanisms involved in this process. Methods: H9C2 rat cardiomyocytes were cultured,and DOX was divided into different concentrations to determine the concentration of DOX.Then they were divided into normal control group,DOX model group and treatment group (low-dose BBR,medium-dose BBR,high-dose BBR) to treat H9C2 cells. The cell growth trend and cell morphology were observed by inverted microscope. The cell viability was determined by cell counting kit (CCK8) method. The apoptosis was detected by flow cytometry. The relative expression levels of apoptosis and autophagy related proteins Bax,Bcl-2,Beclin1 and P62 in different groups were determined by Western-blot. Results: Under the microscope,it was found that with the increase of DOX concentration,the degree of myocardial cell injury increased in turn. CCK8 method was used to select 1 μmol/L DOX as the optimal modeling concentration for myocardial injury,compared with the control group,different concentrations of BBR in the treatment group could increase the viability of DOX-induced myocardial injury cells. The expression of pro-apoptotic protein Bax and autophagy-related protein Beclin1 in the middle dose of the treatment group was significantly decreased,and the expression of anti-apoptotic protein Bcl-2 and autophagy-related protein P62 was increased (P＜0.05). Conclusion: Berberine can protect DOX-induced cardiomyocyte injury by reducing apoptosis,and this protective mechanism is related to autophagy.

Objective: To study the expression of lncRNA SNHG10 and methyltransferase METTL3 in colorectal cancer (CRC) tissues and adjacent tissues. Meanwhile, to explore the potential relationship between lncRNA SNHG10 and METTL3 in CRC. Methods: Tumor tissue and paracancerous tissue samples of 15 CRC patients were collected. The expression of lncRNA SNHG10 and METTL3 in CRC was investigated by bioinformatics, real-time fluorescence quantitative PCR (RT-qPCR) and Western blot. The relationship between the relative expression level of lncRNA SNHG10 in colorectal cancer and prognosis was analyzed. The gene interaction was analyzed by TCGA database, ENCORI, cBioPortal website and in vitro inhibition experiment of colon cancer cell line (HCT116). Results: Bioinformatics website and RT-qPCR experiments showed that lncRNA SNHG10 was up-regulated in colorectal cancer compared with adjacent tissues (P＜0.05). The high expression of lncRNA SNHG10 indicated that CRC patients were more likely to have lymph node metastasis, and the disease-specific survival rate and overall survival rate were relatively low (P＜0.05). RT-qPCR and Western blot results confirmed that METTL3 was highly expressed in CRC tumor tissues. In vitro experiments showed that knockdown of lncRNA SNHG10 in HCT116 cells resulted in down-regulation of METTL3 expression at both mRNA and protein levels (P＜0.05). Conclusion: The high expression of lncRNA SNHG10 is associated with tumor progression and poor prognosis. LncRNA SNHG10 is positively correlated with METTL3 and regulates METTL3 expression. This study suggests that SNHG10 may be a promising CRC biomarker and therapeutic target.

Objective: To observe the effect of berberine (BBR) on doxorubicin (DOX)-induced myocardial injury and its protective effect, and to further explore whether BBR can affect DOX-induced myocardial injury by regulating Wnt/β-catenin pathway. Methods: The rat myocardial cell line H9C2 was used as the research object and divided into Control group, model group, BBR group and LiCl agonist group. The cells in the control group were not treated. The DOX-induced myocardial injury model was established in the model group, BBR group and LiCl agonist group. The BBR group was intervened by BBR, and the LiCl agonist group was intervened by LiCl. CCK-8 method was used to detect the viability of cardiomyocytes. Flow cytometry was used to detect apoptosis. Western blot was used to detect the expression of Wnt/β-catenin pathway protein β-catenin, apoptosis-related proteins Bcl-2 and Bax. Results: (1)CCK-8 method : The cell viability of the model group was lower than that of the Control group (P＜0.05), while the cell viability of the BBR group and the LiCl agonist group was higher than that of the model group (P＜0.05). (2)Flow cytometry: The apoptosis rate of the model group was higher than that of the control group (P＜0.05). The apoptosis rate of BBR group and LiCl agonist group was lower than that of model group (P＜0.05). (3)Western blot: Compared with the Control group, the expression of Bax protein in the model group increased (P＜0.05), the expression of Bcl-2 and β-catenin protein decreased (P＜0.05), and the ratio of Bax/Bcl-2 also increased (P＜0.05). Compared with the model group, the expression of Bax protein in BBR group and LiCl agonist group decreased (P＜0.05), the expression of Bcl-2 protein increased (P＜0.05), the expression of β-catenin protein increased (P＜0.05), and the ratio of Bax/Bcl-2 decreased (P＜0.05). Conclusion: BBR may play a protective role in cardiomyocytes by promoting the expression of β-catenin in damaged cardiomyocytes, up-regulating the activation of Wnt/β-catenin signaling pathway and inhibiting cardiomyocyte apoptosis.

Objective: To observe the effect of quercetin on esophageal mucosal barrier function in rats with gastroesophageal reflux and explore its mechanism. Methods: Forty SD rats were randomly divided into blank control group, model group, quercetin administration group and positive control group. In addition to the blank control group, the rest of the rat model of reflux esophagitis was established. The quercetin administration group was given quercetin 50 mg/kg, the positive control group was given omeprazole 1.52 mg/kg, hydrotalcite 56.89 mg/kg, and domperidone 1.14 mg/kg. The blank control group was given an equal volume of normal saline once a day for 14 consecutive days. After the last administration, the rats were anesthetized and the blood was taken from the abdominal aorta, and the esophageal tissue samples were sacrificed. Then the pathological staining of esophageal tissue samples was performed, and the contents of TNF-α and IL-6 were detected by LISA, and the expression of IL-6 and TNF-α was detected by B. Results: Compared with the blank control group, the esophageal mucosa of the model group had obvious pathological changes, such as white and rough mucosa, defect, squamous epithelial hyperplasia, etc. Compared with the model group, the gastroesophageal histopathological changes in the quercetin treatment group were reduced. Compared with the blank control group, the contents of IL-6 and TNF-α in the model group were significantly increased (P＜0.05). Compared with the model group, the contents of IL-6 and TNF-α in the administration group and the positive control group were significantly decreased (P＜0.05). WB results showed that compared with the model group, the expression of TNF-α and IL-6 in the administration group and the positive control group was significantly decreased (P＜0.01). Conclusion: Quercetin may play a protective role by reducing inflammatory response and promoting gastroesophageal mucosal repair.

Objective: To investigate the expression and clinical significance of serum soluble endothelial protein C receptor (sEPCR) in patients with rheumatoid arthritis (RA). Methods: From August 2022 to May 2023,80 RA patients were admitted to Department of Rheumatology and Immunology,the First Affiliated Hospital of Baotou Medical College,along with 80 healthy individuals as the control (HC) group during the same period. Basic information of all participants,as well as laboratory examination indicators and Disease Activity Score in 28 joints (DAS28) scores of RA patients,were recorded and organized. The levels of serum sEPCR in both groups were measured using enzyme-linked immunosorbent assay (ELISA). The serum sEPCR levels were compared between HC and RA groups,as well as the levels among different disease activities. Spearman analysis was employed to explore the correlation between serum sEPCR levels and clinical and laboratory indicators in RA patients. ROC analysis was performed to assess its clinical diagnostic value. Results: The serum sEPCR levels were significantly increased in the RA group compared to the HC group (P＜0.000 1). Correlation analysis showed that sEPCR levels were positively correlated with age,erythrocyte sedimentation rate (ESR),C-reactive protein (CRP),and DAS28 score (P＜0.001). ROC analysis revealed an area under the curve (AUC) of 0.901 7,with a serum sEPCR level less than 0.693 2 ng/mL as the diagnostic cutoff value,demonstrating a sensitivity of 81.01% and specificity of 88.31%. Conclusion: Serum sEPCR levels are closely related to the disease activity of RA,and have a certain diagnostic value for RA.

Objective: To systematically evaluate the efficacy and adverse reactions of epirubicin (EPI) and mitomycin (MMC) in patients with non-muscle invasive bladder cancer (NMIBC) after perfusion chemotherapy. Methods: CNKI, Wanfang, VIP, SinoMed, PubMed, Embase, The Cochrance Library and Web of science were searched for Chinese and English randomized controlled trials of epirubicin and mitomycin in the treatment of NMIBC from the establishment of the database to January 2023. RevMan 5.3 software was used to perform a meta-analysis of postoperative tumor recurrence rate, total incidence of adverse reactions, incidence of bladder irritation, liver and kidney function damage, and hematuria in the EPI group and the MMC group. Results: There were 16 studies that met the inclusion and exclusion criteria, with a total of 1244 patients, while 626 patients in the EPI group and 618 patients in the MMC group. The results of meta-analysis showed that the postoperative tumor recurrence rate [OR=0.55,95%CI(0.41, 0.73), P＜0.01], the incidence of total adverse reactions [OR=0.56,95%CI(0.38, 0.82), P=0.003], the incidence of bladder irritation [OR=0.54, 95%CI(0.37, 0.77), P=0.0007], the incidence of hematuria [OR=0.53,95%CI(0.33, 0.85), P=0.009], and the incidence of liver and kidney dysfunction [OR=0.26, 95%CI(0.10, 0.69), P=0.007] in the EPI group were significantly lower than those in the MMC group (P＜0.05). Conclusion: EPI is superior to MMC in terms of therapeutic effect and total incidence of adverse reactions. EPI bladder perfusion is recommended to prevent tumor recurrence and improve the prognosis of patients after bladder cancer surgery.

Objective: To investigate the expression level and clinical value of CircRNA-IFT80 in peripheral blood of patients with acute cerebral infarction. Methods: From June 2021 to December 2021, 39 patients with acute cerebral infarction who were admitted to the hospital continuously were selected as the study group, and 44 patients who visited or underwent physical examination in the same hospital during the same period were selected as the control group. The level of CircRNA-IFT80 was detected by real-time fluorescence quantitative PCR, and the correlation between basic data and CircRNA-IFT80 expression level and acute cerebral infarction was analyzed. ROC curve was used to analyze the value of CircRNA-IFT80 in acute cerebral infarction. Results: The expression of Circ RNA-IFT80 in circulating blood of the study group was lower than that of the healthy control group (P＜0.001). The incidence of acute cerebral infarction was significantly correlated with age, sex, systolic blood pressure, diastolic blood pressure, total cholesterol, high density lipoprotein, hypertension history, smoking history, drinking history and CircRNA-IFT80 expression. The expression of CircRNA-IFT80 was a protective factor for acute cerebral infarction. Conclusion: The expression of Circ RNA-IFT80 is different in cerebral infarction, and Circ RNA-IFT80 may play a potential role in the occurrence and development of acute cerebral infarction.

Objective: To observe the clinical effects of Oxford Ⅲ unicompartmental knee arthroplasty (UKA) and total knee arthroplasty (TKA) in the treatment of medial compartment osteoarthritis of the knee joint. Methods: The clinical data of 66 patients with medial compartment osteoarthritis of the knee admitted to the Fourth Hospital of Baotou from January 2018 to January 2021 were selected and divided into observation group and control group (33 patients in each group) according to different surgical methods. The observation group was treated with UKA, and the control group was treated with TKA. The surgical related indexes of the two groups were compared. The femoral-tibial angle (FTA) and range of motion (ROM) of the two groups were compared before and 1 year after operation. The pain and knee function were evaluated by visual analogue scale (VAS) and Hospital for Special Surgery knee score (HSS). And the complications of the two groups. Results: The operation time, incision length and hospitalization time of the observation group were shorter than those of the control group, and the intraoperative blood loss was less than that of the control group (P＜0.05). Before operation, there was no significant difference in FTA, ROM, VAS score and HSS score between the two groups (P＞0.05), while the FTA and VAS scores of the two groups at 1 year after operation were significantly lower than those before operation, and those of the observation group were lower than the control group (P＜0.05). At 1 year after operation, the ROM and HSS scores of the two groups were higher than those before operation, and those of the observation group were higher than the control group (P＜0.05). The total incidence of postoperative complications such as persistent pain, prosthesis shedding, joint stiffness, and lateral compartment osteoarthritis in the observation group (9.09%) was lower than that in the control group (30.30%) (P＜0.05). Conclusion: Compared with TKA, UKA has the advantages of less trauma, shorter operation time, less intraoperative blood loss and faster postoperative recovery in the treatment of medial compartment osteoarthritis of knee joint. Meanwhile, it can improve the femoral-tibial angle, knee joint mobility and knee joint function, effectively reduce the pain of patients, reduce postoperative complications and shorten the hospitalization time. Therefore, it is worthy of popularization and application.

Objective: To study the correlation between lipoprotein a, ApoE gene polymorphism and cardiac valve calcification, and to explore the role of genotype and lipoprotein a in the disease process. Methods: A total of 412 patients admitted to the Department of Cardiology from July 2021 to December 2022 were selected. The results of lipoprotein a and other blood lipids, ApoE genotype, echocardiography and carotid intima-media thickness (IMT) were collected, and the clinical data such as blood pressure were recorded. At the same time, the history of arrhythmia, heart failure, valvular heart disease and ACS were recorded. The correlation between ApoE genotype and blood lipid, cardiac valve calcification was analyzed. Results: The contents of TC, TG, lipoprotein a and LDL-C in the baseline data of the three groups were statistically different. Compared with the ε3 group, the contents of lipoprotein a and LDL-C in the ε2 group were lower, while those in the ε4 group were higher. Univariate analysis showed that age, HDL-C, TG, lipoprotein a, LDL-C, IMT, history of arrhythmia, history of heart failure, history of valvular heart disease were associated with valvular calcification. Binary logistic regression analysis showed that age, lipoprotein a, IMT and history of arrhythmia were independent risk factors for cardiac valve calcification. There was a correlation between lipoprotein a and cardiac valve calcification in the ε2 and ε3 subgroups, but there was no correlation in ε4 subgroup. The area under the curve for the combined diagnosis of IMT and lipoprotein a to predict cardiac valve calcification was 0.760 (P＜0.001). Conclusion: ApoE genotype does not directly affect the occurrence of calcification, but indirectly affects the disease by changing blood lipids. ε4 may be related to the increase of lipoprotein a and LDL-C. ε2 may be related to the decrease of lipoprotein a and LDL-C. Lipoprotein a may be related to the content of LDL-C.

Objective: To investigate the relationship between phosphoglycerate kinase 1 (PGK1) and clinicopathological parameters of breast cancer and its prognostic significance. Methods: (1)The matrix data and clinical data of PGK 1 mRNA expression in breast cancer and normal breast tissues were downloaded from TCGA and GEO databases. The relationship between PGK 1 mRNA expression in breast cancer tissues and clinicopathological features was analyzed. Logistics regression was used to evaluate the independent risk factors of PGK 1 mRNA expression. Kaplan-Meier method was used to draw survival curves to analyze the relationship between PGK 1 mRNA expression level and overall survival (OS) of breast cancer patients. (2)From January 2022 to January 2023, 50 cases of breast cancer and normal breast tissue specimens were collected from the Department of Breast Surgery, the First Affiliated Hospital of Baotou Medical College. The expression levels of PGK1 in breast cancer and normal breast tissues were detected by real-time fluorescence quantitative PCR and immunohistochemical staining, respectively. The relationship between PGK 1 expression and clinicopathological parameters in breast cancer tissues was analyzed, and Logistics regression was used to evaluate the independent risk factors related to PGK1 expression. Results: (1)The expression of PGK1 mRNA in breast cancer was significantly higher than that in normal breast tissue (P＜0.05). (2)ER, PR and HER-2 were independent risk factors for PGK1 mRNA expression. (3)The high expression of PGK 1 mRNA was positively correlated with the decrease of overall survival. (4)The expression of PGK 1 in breast cancer was statistically significant. (5)ER, PR and HER-2 were independent risk factors affecting the expression of PGK1. Conclusion: (1)The expression level of PGK1 in breast cancer is significantly increased. (2)The expression of ER, PR, HER-2 is positively correlated with the expression of PGK1. (3) High expression of PGK1 may be a potential predictor of poor prognosis of breast cancer.