Objective: Improve the quality control standards for the Mongolian medicine preparation Garixi pill. Methods: The experiment employed microscopic identification methods to qualitatively identify the five herbal ingredients-Piper longum, Cinnamomum cassia, Punica granatum, Cinnamomum cassia, and Rheum palmatum-in the Garixi pill formulation. Thin-layer chromatography (TLC) was selected to perform qualitative identification of Piper longum, Cinnamomum cassia, Cinnamon in the Garixi pill. Simultaneously, the content of piperine in the Garixi pill was determined using high-performance liquid chromatography (HPLC). An Elit SinoPak SP column (4.6 × 250 mm, 5 μm) was used, with methanol-water as the mobile phase for gradient elution. The column temperature was set to 30 ℃, the detection wavelength was 343 nm, and the flow rate was controlled at 1.0 mL/min. Results: The microscopic identification characteristics were obvious. The results of TLC identification showed that long pepper fruit, elecampane inula root and cassia bark had clear spots, strong specificity, good separation, negative without interference and easy identification. The peak area of piperine had a good linear relationship in the concentration range of 0.010 5-0.089 8 mg/mL. The regression equation was Y=1 110.7X+1.015 7 (R²=0.999 6). The average recovery was 105.25%, RSD=1.90%. Conclusion: The quality standard analysis method of Mongolian medicine Garixi pill established in this study has the characteristics of simple operation, accurate results, good repeatability and precision, and strong specificity, which can provide a reliable basis for the quality control of the preparation.