目的: 探讨Hsa_cric_0000437在胃癌患者血清中的表达水平及其临床意义。方法: 本研究纳入2022年10月至2023年8月包头医学院第一附属医院经病理确诊的50例胃癌患者及同期50例健康体检者的血清样本。采用RT-qPCR检测Hsa_circ_0000437的表达,通过比较两组Ct值分析其表达差异,并绘制ROC曲线,通过AUC评估其对胃癌的诊断效能。结果: 根据相对定量计算结果(2^-ΔΔCt＞1),胃癌患者血清中Hsa_circ_0000437的表达水平显著高于健康对照。ROC曲线分析显示,其诊断胃癌的AUC为0.816(95%CI: 0.753-0.878),最佳临界值为1.034,此时敏感度为95.6%,特异度为60.0%。结论: Hsa_circ_0000437在胃癌患者血清中高表达,且具备良好的诊断效能,对胃癌的早期诊断和筛查具有重大的临床意义,未来很可能作为一种新型肿瘤标志物。
Objective: To investigate the expression level and clinical significance of Hsa_circ_0000437 in the serum of gastric cancer (GC) patients. Methods: A total of 50 pathologically confirmed GC patients and 50 healthy controls were enrolled from the First Affiliated Hospital of Baotou Medical College between October 2022 and August 2023. Serum Hsa_circ_0000437 expression was detected by RT-qPCR, and the difference in expression between the two groups was analyzed by comparing Ct values. A receiver operating characteristic (ROC) curve was plotted, and the AUC was calculated to evaluate the diagnostic efficacy of Hsa_circ_0000437 for GC. Results: The relative quantitative results (2^-ΔΔCt＞1) indicated that the expression level of Hsa_circ_0000437 in the serum of GC patients was significantly higher than that in healthy controls. ROC analysis showed an AUC of 0.816 (95%CI: 0.753-0.878) for diagnosing GC, with an optimal cutoff value of 1.034, corresponding to a sensitivity of 95.6% and a specificity of 60.0%. Conclusion: Hsa_circ_0000437 is highly expressed in the serum of GC patients and demonstrates good diagnostic performance, indicating significant clinical potential as a novel tumor biomarker for early diagnosis and screening of gastric cancer.
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