目的: 探讨基于生物信息学的三结构域蛋白59(tripartite motif containing 59, TRIM59)基因在卵巢癌中的表达及其临床价值。方法: 根据GEPIA数据库及GEO数据库中的数据集分析TRIM59 mRNA在卵巢癌和卵巢组织中的表达情况。The Human Protein Atlas 数据库用来分析TRIM59蛋白在卵巢癌和卵巢组织中表达情况,Kaplan-Meier数据库研究TRIM59表达高低与卵巢癌患者时间的相关性。在ULACAN数据库和DAVID数据库中筛选出与TRIM59共表达的基因并进行GO分析及KEGG富集分析。TIMER数据库研究卵巢癌组织中TRIM59 mRNA的表达水平与免疫细胞浸润的相关性。结果: TRIM59 mRNA的表达水平在卵巢癌组织中明显高于正常卵巢组织,且这种差异性表达与卵巢癌患者的生存时间密切相关。免疫组化结果显示,TRIM59 蛋白在卵巢癌组织中的阳性率为75%,在正常卵巢组织中未见表达。在卵巢癌组织中,与TRIM59正相关的共表达基因为148个,他们主要参与了DNA复制、蛋白质合成、多糖生物合成及P53信号通路。在卵巢癌中TRIM59蛋白含量与CD4+T淋巴细胞、中性粒细胞、NK细胞的浸润程度呈正相关。结论: TRIM59在卵巢癌组织中呈高表达且与卵巢癌患者生存时间相关,其可作为卵巢癌新的治疗靶点和标志物。
Objective: To explore the expression and clinical value of tripartite motif containing 59 (TRIM59) gene in ovarian cancer based on bioinformatics. Methods: The expression of TRIM59 mRNA in ovarian cancer tissues and ovarian tissues was analyzed by data sets in GEPIA database and GEO database. The expression of TRIM59 protein in ovarian cancer and ovarian tissue was analyzed in The Human Protein Atlas database. Kaplan-Meier database was used to study the correlation between TRIM59 expression and prognosis of ovarian cancer patients. Genes co-expressed with TRIM59 in ovarian cancer were screened from ULACAN database, and GO analysis and KEGG enrichment analysis were performed in DAVID database. The relationship between the expression of TRIM59 mRNA and the infiltration of various immune cells in ovarian cancer was analyzed in TIMER database. Results: The expression level of TRIM59 mRNA in ovarian cancer tissues was significantly higher than that in normal ovarian tissues, and this differential expression was closely related to the survival time of ovarian cancer patients. The results of immunohistochemistry showed that the positive rate of TRIM59 protein in ovarian cancer tissues was 75%, and no expression was found in normal ovarian tissues. In ovarian cancer tissues, 148 co-expressed genes were positively correlated with TRIM59, which were mainly involved in DNA replication, protein synthesis, polysaccharide biosynthesis and P53 signaling pathway. The content of TRIM59 protein in ovarian cancer was positively correlated with the infiltration of CD4+ T lymphocytes, neutrophils and NK cells. Conclusion: This study confirms that TRIM59 is highly expressed in ovarian cancer and closely related to the prognosis of ovarian cancer patients, which can be used as a new therapeutic target and diagnostic marker.
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