目的:探讨在颈动脉粥样硬化(CAS)中circRNA-GRHPR的表达水平及临床意义。方法:随机选择在包头医学院第一附属医院神经内三科住院患者124例,根据颈动脉影像学检查是否存在动脉粥样硬化(AS)分为对照组和病例组。对照组30例患者,颈动脉中并未发现斑块;病例组94例患者,颈动脉中存在着斑块,这些斑块或是硬质的,或是软质的,又或是软硬混合的。应用实时荧光定量PCR(qRT-PCR)验证circRNA-GRHPR在细胞中具有差异化表达,深入分析circRNA-GRHPR在CAS中所起的作用。结果:CAS的发生与性别、年龄、收缩压、吸烟史、饮酒史、高血压病史、外周血circRNA-GRHPR表达水平均相关,且均为正相关(P＜0.05);HDL-C与CAS相关,且为负相关(P＜0.05)。病例组患者外周血circRNA-GRHPR表达水平高于对照组(P＜0.05)。结论:CAS的发生与患者血清中circRNA-GRHPR的表达水平具有明显相关性,有可能成为AS改进诊断的工具和新治疗靶点,为早期干预措施铺平道路。
Objective: To investigate the expression levels and clinical significance of circRNA-GRHPR in carotid atherosclerosis (CAS). Methods: A total of 124 patients were randomly selected from the Third Department of Neurology, the First Affiliated Hospital of Baotou Medical College. These patients were divided into the control group and the case group based on whether they had atherosclerosis (AS) in the carotid artery as shown by imaging examinations. In the control group, 30 patients had no plaques found in the carotid arteries; in the case group, 94 patients had plaques in the carotid arteries, which were either hard, soft, or mixed. Real-time quantitative PCR (qRT-PCR) was used to verify differential expression of circRNA-GRHPR in cells and to further analyze the role of circRNA-GRHPR in CAS. Results: The occurrence of CAS was associated with gender, age, systolic blood pressure, smoking history, alcohol consumption history, hypertension history, and peripheral blood circRNA-GRHPR expression levels, all of which were positively correlated (P＜0.05); HDL-C was associated with CAS but negatively correlated (P＜0.05). The peripheral blood circRNA-GRHPR expression level in the case group was higher than that in the control group (P＜0.05). Conclusion: The occurrence of CAS is significantly related to the expression levels of circRNA-GRHPR in patients′ serum, potentially serving as an improved diagnostic tool and a new therapeutic target for AS, paving the way for early intervention measures.
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