目的: 从北五味子果实中分离筛选对大肠杆菌、金黄色葡萄球菌、白色念珠菌具有抑菌活性的内生菌,为临床新型抗菌药物的研发提供更多的筛选源。方法: 采用平板对峙法、牛津杯法检测内生菌对3种供试菌的抑菌活性,通过SPSS 20.0软件进行数据统计分析,并进一步通过形态学观察、生理生化特征、分子生物学方法鉴定内生菌的菌属。结果: 共分离得到25株内生菌,其中菌株GF20发酵液对三种供试菌均具有显著抑菌活性。经鉴定确定GF20为芽孢杆菌属细菌。结论: 北五味子果实中含有丰富的内生菌资源,内生菌对常见病原菌具有不同的抑菌活性,具备新型抗菌药物开发的潜力。
Objective: The endophytes with antibacterial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans are isolated and screened from Schisandra chinensis fruit, providing more screening sources for developing new clinical antibacterial drugs. Methods: The inhibitory activities of the endophytes against the three test pathogens were detected by the plate standoff method and Oxford cup method, and the data were statistically analyzed by SPSS Statistics 20.0 software, and the genera of the endophytes were further identified by morphological observation, physiological and biochemical characteristics, and molecular biology methods. Results: 25 endophytic strains were isolated, among which the fermentation broth of strain GF20 had strong antibacterial activity against the three tested pathogenic bacteria. After identification, GF20 was determined to be a bacterium of the genus Bacillus. Conclusion: Schisandra chinensis fruits are rich in endophytic bacteria, which have different antibacterial activities against common pathogenic bacteria and can potentially develop new antibacterial drugs.
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