目的: 探究甲羟戊酸二磷酸脱羧酶a(mevalonate diphosphate decarboxylase a, mvda)对斑马鱼心肌再生的调控作用。方法: 分别将对照组(DMSO)、MTZ处理的心肌损伤组(MTZ),敲低mvda并用MTZ处理组(mvda MO+MTZ)和过表达mvda后MTZ处理组(mvda OE+MTZ)的斑马鱼胚胎在受精后第6 天,用激光共聚焦显微镜分别检测斑马鱼心肌再生情况及其心率。结果: 加入MTZ导致斑马鱼心肌消融,敲减mvda的斑马鱼心肌再生效率相较MTZ组发生明显下降(P＜0.05);敲减mvda的斑马鱼心肌再生受抑制,过表达mvda的斑马鱼心肌再生比例高于MTZ损伤组(MTZ)(P＜0.05);过表达mvda的斑马鱼相较于MTZ损伤组和敲低mvda的斑马鱼,心率恢复至正常水平的比例较高(P＜0.05)。结论: mvda能够调控斑马鱼心肌再生,过表达mvda的斑马鱼促进斑马鱼心肌再生,并促进心脏功能恢复。
陈向辉
,
林玉凤
,
李晓婷
,
吕学浩
,
孙嘉骏
,
谷欣悦
,
王雯萱
,
李一民
,
贾小娥
,
朱伟
. 甲羟戊酸二磷酸脱羧酶a调控斑马鱼心肌再生*[J]. 包头医学院学报, 2025
, 41(7)
: 44
-48
.
DOI: 10.16833/j.cnki.jbmc.2025.07.008
Objective: To explore the effect of mevalonate diphosphate decarboxylase a (mvda) on the myocardial regeneration of zebrafish. Methods: On the 6th day after fertilization, the zebrafish embryos of the control group (DMSO), MTZ-treated myocardial injury group (MTZ), MTZ-treated group after mvda knock-out (mvda MO+MTZ) and MTZ-treated group after mvda overexpression (mvda OE+MTZ) were detected by confocal laser scanning microscopy. Results: Adding MTZ led to myocardial ablation of zebrafish. Compared with the MTZ group, mvda knocked-down could inhibit myocardial regeneration of zebrafish(P＜0.05), while regeneration ratio of ventricular in zebrafish with overexpression of mvda was higher(P＜0.05). It was found that zebrafish with overexpression of mvda had a higher rate of heart rate recovery to normal level than those with MTZ injury and mvda knocked down (P＜0.05). Zebrafish with overexpression of mvda could improve myocardial regeneration and promote recovery of cardiac function. Conclusion: Mvda can regulate the myocardial regeneration of zebrafish.
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