目的:观察蒙药香青兰总黄酮(TFDM)对大鼠脑缺血再灌注损伤后缺血半暗带区微管相关蛋白-2(MAP-2)表达的影响,探讨其对神经可塑性的作用。方法:采用SD大鼠构建大脑中动脉闭塞再灌注模型,实验分为假手术组(Sham)、缺血再灌注组(I/R)和TFDM给药组(TFDM),手术后第1、3、7、14天 4个时相进行取材,免疫组织化学染色观测MAP-2阳性标记的细胞及树突在皮层缺血半暗带区的表达情况,Western Blot检测皮层缺血半暗带区MAP-2蛋白的表达情况。结果:免疫组织化学和Western Blot结果显示,再灌注后第3天和第7天时,TFDM组大鼠皮层缺血半暗带MAP-2表达量显著高于I/R组(P＜0.05)。结论:TFDM可能通过上调MAP-2蛋白的表达,增强脑缺血半暗带区神经可塑性调节,促进树突生长及受损神经结构修复,发挥神经保护作用。
Objective: To observe the effect of Mongolian medicine total flavonoids of Dracocephalum moldavica L (TFDM) on the expression of microtubule-associated protein-2 (MAP-2) in the ischemic penumbra of rats after cerebral ischemia-reperfusion, and explore its role in neural plasticity. Methods: Constructing a middle cerebral artery occlusion reperfusion model using SD rats. The experiment was divided into sham surgery group (Sham), ischemia-reperfusion group (I/R), and TFDM administration group (TFDM). Samples were collected at four time points: 1, 3, 7, 14 days after surgery. The immunohistochemical staining was used to observe the expression of MAP-2 positive labeled cells and dendrites in the ischemic penumbra of the cortex, and Western blot was used to detect the expression of MAP-2 protein in the ischemic penumbra of the cortex. Results: The results of immunohistochemistry and Western Blot showed that the expression of MAP-2 in the ischemic penumbra of the TFDM group was significantly higher than that in the I/R group on the 3rd and 7th day after reperfusion (P＜0.05). Conclusion: TFDM may play a neuroprotective role by up-regulating the expression of MAP-2 protein, enhancing the regulation of neural plasticity in the ischemic penumbra, promoting dendritic growth and repairing damaged nerve structure.
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