目的: 探讨C5a受体(C5aR1)、缓激肽受体B1(bradykinin receptor B1, BDKRB1)、缺氧诱导因子-1α(hypoxia inducible factor-1α, HIF-1α)、G0/G1开关2(G0/G1 switch 2, G0S2)基因在肠型胃癌Correa发展模式中的表达特点,筛选胃癌前病变进展的预测因子。方法: 选取慢性非萎缩性胃炎(chronic non-atrophic gastritis, CNAG)、慢性萎缩性胃炎(chronic atrophic gastritis, CAG)、低级别上皮内瘤变(low-grade intraepithelial neoplasia, LGIN)、高级别上皮内瘤变(high-grade intraepithelial neoplasia, HGIN)、侵及黏膜层及黏膜下层的早期胃癌(early gastric cancer, EGC)组织蜡块,应用免疫组化法检测各组织中C5aR1、BDKRB1、G0S2及HIF-1α的蛋白表达水平。结果: C5aR1主要表达于细胞膜与细胞质中,BDKRB1主要表达于细胞膜中,G0S2和HIF-1α主要表达于细胞质与细胞核中。HGIN、EGC组织中C5aR1、 BDKRB1、G0S2的表达水平显著高于CAG和CNAG(P＜0.001)。HGIN、EGC组织中HIF-1α表达水平显著高于CNAG(P＜0.001),但与CAG相比差异无统计学意义。4个因子在LGIN与其他组织中的表达结果相比差异均无统计学意义,在CNAG和CAG组织中的表达结果相比差异无统计学意义。结论: C5aR1、BDKRB1、G0S2和HIF-1α表达量随着肠型胃癌Correa发展阶段呈增加趋势,可能具有预测胃癌前病变进展的价值。
Objective: To explore the expression characteristics of C5a receptor (C5aR1), bradykinin receptor B1 (BDKRB1), hypoxia inducible factor-1α (HIF-1α) and G0/G1 switch 2 (G0S2) genes in the Correa development model of intestinal gastric cancer, and to screen the predictors of the progression of gastric precancerous lesions. Methods: The paraffin blocks of chronic non-atrophic gastritis (CNAG), chronic atrophic gastritis (CAG), low-grade intraepithelial neoplasia (LGIN), high-grade intraepithelial neoplasia (HGIN), early gastric cancer (EGC) and early gastric cancer in submucosa were selected. The protein expression levels of C5aR1, BDKRB1, G0S2 and HIF-1α in each tissue were detected by immunohistochemistry. Results: C5aR1 was mainly expressed in cell membrane and cytoplasm, BDKRB1 was mainly expressed in cell membrane, G0S2 and HIF-1α were mainly expressed in cytoplasm and nucleus. The expression levels of C5aR1, BDKRB1 and G0S2 in HGIN and EGC tissues were significantly higher than those in CAG and CNAG tissues(P＜0.001). The expression level of HIF-1α in HGIN and EGC tissues was significantly higher than that in CNAG(P＜0.001), but there was no statistical difference compared with CAG. There was no significant difference in the expression of the four factors between LGIN and other tissues, and there was no significant difference in the expression of CNAG and CAG tissues. Conclusion: The expressions of C5aR1, BDKRB1, G0S2 and HIF-1α increase with the Correa development stage of intestinal type gastric cancer, which may have the value of predicting the progression of gastric precancerous lesions.
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