目的: 观察核糖激酶(ribokinase, Rbks)基因敲除对C57BL/6J小鼠表型和糖脂代谢的影响。方法: 分别取12只雄性8周龄野生(wide type, WT)小鼠和Rbks基因敲除(knockout, KO)C57BL/6J小鼠,正常饲喂10周,定期观察小鼠外观、体重、摄食、摄水及空腹血糖的变化,观察期结束后,取血清测定甘油三酯和胆固醇含量。结果: 正常饲喂10周,KO小鼠与WT小鼠相比,外观与体质量差异无统计学意义(P＞0.05),但摄食量与摄水量降低,空腹血糖水平明显升高(P＜0.001),血清甘油三酯含量升高(P＜0.05)、总胆固醇含量降低(P＜0.05)。结论: Rbks基因敲除虽然对小鼠外形体重无影响,但会影响小鼠体内的糖脂代谢平衡。
杨忠彬
,
郭雅静
,
王泽颖
,
陈子儒
,
李厚宇
,
何崎
,
刘佳慧
,
马士博
,
居红格
,
李晓晶
,
刘治
,
苏燕
. Rbks基因敲除对C57小鼠糖脂代谢的影响*[J]. 包头医学院学报, 2025
, 41(5)
: 33
-36
.
DOI: 10.16833/j.cnki.jbmc.2025.05.006
Objective: To observe the effects of ribokinase (Rbks) gene knockout on phenotype and glucose and lipid metabolism in C57BL/6J mice. Methods: 12 male 8-week-old wide type (WT) and Rbks gene knockout (KO) C57BL/6J mice were fed for 10 weeks. The changes of appearance, body weight, food intake, water intake and fasting blood glucose were observed regularly. At the end of the observation period, the contents of triglyceride and cholesterol were measured. Results: After being fed normally for 10 weeks, compared with WT mice, KO mice had no significant difference in appearance and body weight(P＞0.05), but food intake and water intake decreased, fasting blood glucose level increased significantly(P＜0.05), serum triglyceride content increased and total cholesterol content decreased (all P＜0.05). Conclusion: Although Rbks gene knockout has no effect on the shape and weight of mice, it will affect the balance of glucose and lipid metabolism in mice.
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