目的: 基于生物分析探讨miR-27a-3p在慢性间歇低氧(chronic intermittent hypoxia,CIH)中的表达及参与胰岛素抵抗的作用机制。方法: 从GEO中下载CIH条件下miRNA的表达数据集,使用R软件EdgeR包进行差异miRNA筛选,选取差异miR-27a-3p作为研究对象。运用TargetScan、miRTarbase、miRDB对miR-27a-3p进行靶基因预测并取得交集进行GO和KEGG富集分析,通过genecard检索胰岛素抵抗的基因集,与miR-27a-3p的交集靶基因取交集,然后构建miR-27a-3p靶基因的PPI网络,筛选中枢基因,并构建miRNA-mRNA-pathway调控网络。结果: GSE210021数据集中共筛选出37个差异表达的miRNA,其中miR-27a-3p是下调基因之一。3个数据库中miR-27a-3p交集靶基因505个,主要参与蛋白质丝氨酸/苏氨酸激酶活性的调控等生物学过程,参与MAPK信号通路等途径。胰岛素抵抗基因集与miR-27a-3p的交集靶基因有137个,中枢基因有EGFR等。miRNA-mRNA-pathway显示miR-27a-3p是MAPK信号通路等的关键调控因子。结论: 慢性间歇低氧下miR-27a-3p表达下调,其可能通过作用于靶基因MAPK14等参与胰岛素抵抗的发生,机制可能涉及到MAPK等靶点的相关信号通路。
Objective: To investigate the expression of miR-27a-3p in chronic intermittent hypoxia (CIH) and its involvement in insulin resistance through biological analysis. Methods: Download the miRNA expression dataset under CIH conditions from GEO, use the EdgeR package of R software for differential miRNA screening, and select differential miR-27a-3p as the research object. Using Target Scan, miRRisk, and miRDB to predict the target genes of miR-27a-3p and obtain the intersection for GO and KEGG enrichment analysis. The gene set of insulin resistance was retrieved through genecard, and the intersection of miR-27a-3p target genes was taken. Then, a PPI network of miR-27a-3p target genes was constructed, central genes were screened, and a miRNA mRNA pathway regulatory network was constructed. Results: a total of 37 differentially expressed miRNAs were screened from the GSE210021 dataset, among which miR-27a-3p was one of the down regulated genes. There are 505 intersecting target genes of miR-27a-3p in three databases, mainly involved in the regulation of protein serine/threonine kinase activity and other biological processes, as well as the MAPK signaling pathway. There are 137 intersecting target genes between the insulin resistance gene set and miR-27a-3p, including central genes such as EGFR. The miRNA mRNA pathway shows that miR-27a-3p is a key regulatory factor in the MAPK signaling pathway and other pathways. Conclusion: Under chronic intermittent hypoxia, miR-27a-3p expression is downregulated, which may be involved in the occurrence of insulin resistance by acting on target genes such as MAPK14. The mechanism may involve related signaling pathways of MAPK and other targets.
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