目的: 观察火针对膝骨关节炎(knee osteoarthritis, KOA)大鼠膝关节软骨形态的影响,探讨火针治疗KOA的机制。方法: 将21只SD大鼠分为造模组13只和对照组8只,造模组用4%木瓜蛋白酶0.2 mL膝关节腔注射,两组各随机取3只进行造模鉴定。将造模组剩余10只大鼠随机分为模型组和火针组各5只。火针组进行火针治疗,隔天1次,治疗2周;模型组不予治疗。干预后肉眼观察大鼠膝关节软骨情况;苏木素-伊红染色法、番红-固绿染色法观察大鼠膝关节软骨形态。结果: 造模后,造模组软骨表面较对照组粗糙,软骨细胞较对照组裸露、排列紊乱且可见簇集现象,软骨结构较对照组紊乱,结构不清晰;治疗后,对照组软骨表面光滑,有光泽;模型组软骨表面粗糙,缺少光泽;火针组软骨表面稍粗糙,光泽度较模型组明显;HE染色、番红0-固绿染色结果显示,对照组软骨表面平整,软骨外基质染色均匀,结构清晰,潮线完整,模型组出现表面粗糙,软骨细胞裸露、排列紊乱、潮线扭曲等病理改变,火针组病理改变较轻。结论: 火针疗法能够减轻大鼠膝关节软骨损伤,有效治疗膝骨关节炎。
Objective: To observe the effect of fire needle on the cartilage morphology of knee joint in rats with knee osteoarthritis (KOA), and to explore the mechanism of fire needle in the treatment of KOA. Methods: Twenty-one SD rats were divided into model group (n=13) and control group (n=8). The model group was injected with 0.2 mL of 4% papain into the knee joint cavity. Three rats were randomly selected from each group for modeling identification. The remaining 10 rats in the model group were randomly divided into model group and fire needle group, with 5 rats in each group. The fire needle group was treated with fire needle once every other day for 2 weeks. The model group was not treated. After intervention, the cartilage of rat knee joint was observed by naked eye, and the morphology of rat knee joint cartilage was observed by hematoxylin-eosin staining and safranin-fast green staining. Results: After modeling, the cartilage surface of the model group was rougher than that of the control group, and the chondrocytes were exposed, disordered and clustered compared with the control group. The cartilage structure was disordered compared with the control group, and the structure was not clear. After treatment, the cartilage surface of the control group was smooth and shiny; the cartilage surface of the model group was rough and lack of luster; the cartilage surface of the fire needle group was slightly rough, and the glossiness was more obvious than that of the model group. The results of hematoxylin-eosin staining and safranin O-fast green staining showed that the cartilage surface of the control group was flat, the extracellular matrix of the cartilage was evenly stained, the structure was clear, and the tidal line was intact. The model group showed rough surface, exposed chondrocytes, disordered arrangement, tidal line distortion and other pathological changes. The pathological changes of the fire needle group were lighter. Conclusion: Fire needle therapy can alleviate the knee cartilage injury and effectively treat knee osteoarthritis in rats.
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