目的:探究石榴籽油(pomegranate seed oil, PSO)和鱼油(fish oil, FO)对膝骨性关节炎(knee osteoarthritis, KOA)大鼠膝关节软骨IL-1βmRNA表达的影响因素,比较石榴籽油和鱼油对膝关节性关节炎的作用关系,为KOA防治拓展新的思路并提供科学依据。方法:选取8周健康雌性SD大鼠154只,将实验动物用EXCEL随机分成7组:假手术(Sham)组、模型(Model)组、生理盐水(NS)组、石榴籽油低剂量(PSOLD)组、石榴籽油高剂量(PSOHD)组、鱼油低剂量(FOLD)组、鱼油高剂量(FOHD)组,每组22只。采用改良Hulth法手术建立大鼠KOA模型,选取有代表性的两种ω-3多不饱和脂肪酸(n-3PUFAs)PSO和FO实验组干预,Sham组与Model组不做干预,NS组大鼠每天使用生理盐水1 mL/kg灌胃,PSOLD组、PSOHD组分别给予0.83 mL/kg、1.66 mL/kg剂量PSO灌胃,FOLD组、FOHD组分别予0.69 mL/kg、1.38 mL/kg剂量FO灌胃。干预8周后,每组随机处死10只,取大鼠后肢膝关节软骨标本,进行RT-PCR;干预 12周后,处死各组剩余10只大鼠,处理方式同上。结果:与Sham组比,Model组及各试验组8周、12周IL-1βmRNA相对表达量显著升高(P＜0.01);与Model组相比,PSO和FO组8周、12周IL-1βmRNA相对表达量均显著降低(P＜0.01);其余各组差异无统计学意义(P＞0.05)。Model组和NS组8周与12周IL-1βmRNA相对表达量对比有显著增加(P＜0.05),其余各组差异无统计学意义(P＞0.05)。结论:(1)石榴籽油和鱼油都可以有效降低IL-1βmRNA相对表达量,使软骨退变得到改善;(2)相同干预时长的PSOLD组和PSOHD组,FOLD组和FOHD组,下调IL-1βmRNA表达水平的作用均无显著性差别;(3)从8周和12周数据分析对比,两组PSO组和FO组对IL-1βmRNA表达量与干预时长相关性不显著。
吕腾辉
,
张涛
,
邢嘉璐
,
栗蕊
,
祁赫东
,
陈洲洋
,
石庆田
,
马志朋
,
李渊
,
徐志雄
,
许文胜
. 石榴籽油和鱼油对IL-1β介导的膝骨性关节炎软骨退变的影响及作用*[J]. 包头医学院学报, 2025
, 41(2)
: 18
-23
.
DOI: 10.16833/j.cnki.jbmc.2025.02.004
Objective: To explore the influencing factors of pomegranate seed oil and fish oil on the expression of IL-1βmRNA in knee cartilage in rats with osteoarthritis (OA), and to compare the effects of pomegranate seed oil and fish oil on knee arthritis (KOA), so as to expand new ideas and provide scientific basis for the prevention and treatment of KOA. Methods: A total of 154 8-week-old healthy female SD rats were selected, and the experimental animals were randomly divided into 7 groups by EXCEL: Sham group, Model group, normal saline (NS) group, pomegranate seed oil low-dose (PSOLD) group, pomegranate seed oil high-dose (PSOHD) group, fish oil low-dose (FOLD) group, and fish oil high-dose (FOHD) group, with 22 rats in each group. The modified Hulth method was used to establish the rat KOA model, and the representative two omega-3 polyunsaturated fatty acids (n-3PUFAs), pomegranate seed oil (PSO) and fish oil (FO) experimental groups were selected for intervention, and the Sham group and the Model group did not intervene, the rats in the NS group were given 1 ml/kg of normal saline per day, and the PSOLD group and PSOHD group were given 0.83 ml/kg and 1.66 ml/kg PSO gavage, respectively. The FOHD group was given 0.69 ml/kg and 1.38 ml/kg by gavage, respectively. After 8 weeks of intervention, 10 rats in each group were randomly sacrificed, and the cartilage specimens of the knee joint of the hind limbs of the rats were taken for RT-PCR. After 12 weeks of intervention, the remaining 10 rats in each group were sacrificed, and the treatment was the same as above. Results: Compared with the Sham group, the relative expression of IL-1β mRNA in the Model group and the experimental groups was significantly increased at 8 weeks and 12 weeks (P＜0.01). Compared with the Model group, the relative expression of IL-1β mRNA in the PSO and FO groups was significantly decreased at 8 and 12 weeks (P＜0.01). There was no significant difference among the other groups (P＞0.05). The relative expression of IL-1β mRNA in Model group and NS group increased significantly at 8 weeks and 12 weeks (P＜0.05), while there was no significant difference between the other groups (P＞0.05). Conclusion: (1) Pomegranate seed oil and fish oil can effectively reduce the relative expression of IL-1βmRNA and improve cartilage regression. (2) There is no significant difference in the effect of down-regulating IL-1βmRNA expression between the PSOLD group and the PSOHD group, the FOLD group and the FOHD group with the same intervention duration. (3) From the analysis of 8-week and 12-week data, there is no significant correlation between the expression of IL-1β mRNA and the length of intervention in the two groups of PSO and FO groups.
[1] Martel-Pelletier J, Barr AJ, Cicuttini FM, et al. Osteoarthritis[J]. Nat Rev Dis Primers, 2016, 2: 16072.
[2] Cross M, Smith E, Hoy D, et al. The global burden of hip and knee osteoarthritis: estimates from the global burden of disease 2010 study[J]. Ann Rheum Dis, 2014, 73(7): 1323-1330.
[3] Loeser RF, Collins JA, Diekman BO. Ageing and the pathogenesis of osteoarthritis[J]. Nat Rev Rheumatol, 2016, 12(7): 412-420.
[4] 谷艳超, 刘世清, 夏韶强, 等. 骨关节炎发病机制和治疗的研究进展[J]. 中国骨与关节杂志, 2016, 5(10): 770-774.
[5] 李鹏宇. SMOC2在膝骨关节炎患者中的表达及骨关节炎进程中的作用研究[D]. 济南: 山东大学, 2020.
[6] 乔盈盈, 蒲金呈, 高蓉琳, 等. 脂代谢紊乱在肥胖相关骨性关节炎病程进展中作用的研究进展[J]. 中国骨与关节损伤杂志, 2022, 37(4): 439-442.
[7] Calder PC. Omega-3 fatty acids and inflammatory processes: from molecules to man[J]. Biochem Soc Trans, 2017, 45(5): 1105-1115.
[8] Simopoulos AP. The importance of the ratio of omega-6/omega-3 essential fatty acids[J]. Biomed Pharmacother, 2002, 56(8): 365-379.
[9] Mortensen MS, Ruiz J, Watts JL. Polyunsaturated fatty acids drive lipid peroxidation during ferroptosis[J]. Cells, 2023, 12(5): 804.
[10] Bazinet RP, Layé S. Polyunsaturated fatty acids and their metabolites in brain function and disease[J]. Nat Rev Neurosci, 2014, 15(12): 771-785.
[11] Chen P, Zhou J, Ruan A, et al. Synovial tissue-derived extracellular vesicles induce chondrocyte inflammation and degradation via NF-κB signalling pathway: An in vitro[J]. J Cell Mol Med, 2022, 26(7): 2038-2048.
[12] 魏伟, 吴希美, 李元建, 等. 药理实用方法学[M]. 北京:人民卫生出版社, 2010: 220-222.
[13] Xu B, Bai B, Sha S, et al. Interleukin-1β induces autophagy by affecting calcium homeostasis and trypsinogen activation in pancreatic acinar cells[J]. Int J Clin Exp Pathol, 2014, 7(7): 3620-3631.
[14] 黄金刚, 童海骏, 刘宏强, 等. IL-1β和TNF-α对软骨细胞基质降解的影响及相关机制研究[J]. 上海交通大学学报(医学版), 2010, 30(9): 1084-1089.
[15] Ashraf S, Cha BH, Kim JS, et al. Regulation of senescence associated signaling me,chanisms in chondrocytes for cartilage tissue regeneration[J]. Osteoarthritis Cartilage, 2016, 24(2): 196-205.
[16] Jenei-Lanzl Z, Meurer A, Zaucke F. Interleukin-1β signaling in osteoarthritis-chondrocytes in focus[J]. Cell Signal, 2019, 53: 212-223.
[17] Loef M, Schoones JW, Kloppenburg M, Ioan-Facsinay A. Fatty acids and osteoarthritis: different types, different effects[J]. Joint Bone Spine, 2019, 86(4): 451-458.
[18] 张涛, 许文胜, 刘雄伟, 等. 石榴籽油对骨性关节炎大鼠软骨和软骨下骨元素及血清氧化应激指标的影响及意义[J]. 现代医学, 2019, 47(12): 1476-1481.
[19] Abshirini M, Ilesanmi-Oyelere BL, Kruger MC. Potential modulatory mechanisms of action by long-chain polyunsaturated fatty acids on bone cell and chondrocyte metabolism[J]. Prog Lipid Res, 2021, 83: 101113.
