目的:研究干燥综合征(sjögren's syndrome,SS)患者外周血中低氧诱导因子-1α(hypoxia inducible factor-1α, HIF-1α)的表达水平,分析HIF-1α的表达水平与SS患者实验室指标和口干症状的相关性。方法:选取64例SS患者和64例健康体检者。PCR法检测两组外周血单个核细胞(peripheral blood mononuclear cell, PBMC)中HIF-1α mRNA的表达水平;ELISA法检测血清中HIF-1α蛋白的表达水平;检测SS患者的免疫球蛋白(immunoglobulin, Ig)、红细胞沉降率(erythrocyte sedimentation rate, ESR)、白细胞(white blood cell, WBC)、血红蛋白(hemoglobin, Hb)等指标。结果:与健康对照组相比,SS患者PBMC中HIF-1α mRNA的表达水平增加(P＜0.05);SS患者血清中HIF-1α的表达水平与ESR、IgG、IgM呈正相关(r1=0.457 6、r2=0.426 9、r3=0.403 9,P＜0.05),与WBC、Hb呈负相关(r1=-0.333 3、r2=-0.312 5, P＜0.05)。口干阳性SS患者的HIF-1α蛋白的表达水平高于口干阴性者(P＜0.01)。ROC曲线分析HIF-1α mRNA和蛋白的表达水平对SS的诊断价值,AUC分别为0.659 4和0.813 4,敏感度分别为64.1%和76.6%,特异度分别为95.3%和81.2%。结论:SS患者外周血中HIF-1α的表达水平增高,与ESR、IgG、IgM呈正相关,与WBC、Hb呈负相关,SS患者中口干阳性患者血清中HIF-1α的表达水平高于阴性者,说明HIF-1α对SS有一定的诊断价值,并在SS发病中发挥作用。
Objective: To analyze the correlation between the expression level of hypoxia inducible factor-1α (HIF-1α)and relevant laboratory indicators and dry mouth symptoms in Sjögren's syndrome(SS) patients by comparing the expression levels of HIF-1α in peripheral blood between healthy individuals and patients with SS.Methods: A total of 64 SS patients and 64 health examiners were selected. PCR was used to detect the expression level of HIF-1α mRNA in peripheral blood mononuclear cells (PBMC) of the two groups. ELISA method was used to detect the expression level of HIF-1α protein in serum. Immunoglobulin (Ig), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), white blood cell (WBC), hemoglobin (Hb) and other indicators from SS patients were collected. Results: Compared with the healthy control group, the expression levels of HIF-1α mRNA in PBMC were increased (P＜0.05). HIF-1α expression level in serum of SS patients was positively correlated with ESR, IgG and IgM (r1=0.457 6, r2=0.426 9, r3=0.403 9, P＜0.05), but negatively correlated with WBC and Hb(r1=-0.333 3, r2=-0.312 5, P＜0.05. The expression level of HIF-1 protein in positive dry mouth patients was higher than that in negative dry mouth patients (P＜0.01). The diagnostic value of the expression level of HIF-1α mRNA and protein on SS was analyzed using ROC curves, and the AUC was 0.659 4 and 0.813 4, with the sensitivity of 64.10% and 76.60%, and the specificity of 95.30% and 81.20%, respectively. Conclusion: The expression level of HIF-1α mRNA and protein were higher in PBMC of SS patients. It is positively correlated with ESR, IgG and IgM, while negatively correlated with WBC and Hb. The expression level of HIF-1α in SS patients with positive dry mouth was higher than that of SS patients with negative dry mouth, indicating that HIF-1α has certain diagnostic value in SS and plays a role in the occurrence of SS.
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