目的:探究血管源性脑白质高信号患者外周血单个核细胞中的m6A甲基化调节酶及脑小血管病相关基因的表达情况,为血管源性脑白质高信号的发病机制提供理论依据。方法:选取2021年10月至2022年10月就诊于内蒙古科技大学包头医学院第一附属医院神经内科的脑白质高信号患者和健康体检者各12例。采用实时荧光定量PCR(RT-qPCR)技术分析m6A甲基化调节酶及脑小血管相关基因的mRNA表达情况,蛋白印迹法(Western blot)检测METTL3、ILF3的蛋白表达情况。结果:与对照组相比,METTL3、KAA1429及ILF3的mRNA呈低水平表达(P＜0.05);与对照组相比,METTL3和ILF3的蛋白呈低水平表达(P＜0.05)。与对照组相比,METTL14、DGCR8、FTO、WTAP、RBM15、YTHDC1、ATF3和ERBIN的mRNA表达水平差异无统计学意义(P＞0.05)。结论:甲基化转移酶METTL3和ILF3可能介导了血管源性脑白质高信号的发病。
Objective: To explore the expression of m6A methylation regulatory enzymes and cerebral small vessel-disease related genes in peripheral blood mononuclear cells of patients with vasogenic white matter hyperintensity, and to provide a theoretical basis for the pathogenesis of vasogenic white matter hyperintensity. Methods: From October 2021 to October 2022, 12 patients with white matter hyperintensity and 12 healthy subjects were selected from the Department of Neurology, the First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology. Real-time fluorescence quantitative PCR (RT-qPCR) was used to analyze the mRNA expression of m6A methylation regulatory enzymes and brain small vessel-related genes. Western blot was used to detect the protein expression of METTL3 and ILF3. Results: Compared with the control group, the mRNA expression levels of METTL3, KAA1429 and ILF3 were lower(P＜0.05). Compared with the control group, the protein expression levels of METTL3 and ILF3 were lower(P＜0.05). Compared with the control group, there was no significant difference in the mRNA expression levels of METTL14, DGCR8, FTO, WTAP, RBM15, YTHDC1, ATF3 and ERBIN(P＞0.05). Conclusion: Methyltransferases METTL3 and ILF3 may be involved in the pathogenesis of vasogenic white matter hyperintensity.
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