目的:讨论急性脑梗死患者外周血中circRNA-SRRM2的表达水平及临床意义。方法:收集46例发病时间＜7 d的急性脑梗死患者为病例组,48例非脑梗死患者作为对照组,采用实时荧光定量PCR(real-time quantitative polymerase chain reaction,qRT-PCR)技术检测外周血中circRNA-SRRM2在病例组和对照组中的表达水平,应用spearman及pearson相关分析法分析目的基因与急性脑梗死的关联程度;收集两组患者的一般资料,应用Logistic回归分析法急性脑梗死发病的独立危险因素。结果:外周血中circRNA-SRRM2在急性脑梗死患者组中的表达水平与对照组相比有明显上调(P＜0.05),且性别、年龄、收缩压、舒张压、高血压病史、吸烟史、饮酒史及外周血circRNA-SRRM2表达水平与急性脑梗死发生均呈正相关(P＜0.05)。Logistic回归分析提示急性脑梗死发病的独立危险因素有性别、年龄、收缩压、舒张压、高血压病史、吸烟史、饮酒史及外周血circRNA-SRRM2(P＜0.05),外周血circRNA-SRRM2诊断急性脑梗死的ROC曲线下面积为0.683(P＜0.002),临界值为1.673,敏感度为0.820,特异度为0.470。结论:circRNA-SRRM2在急性脑梗死中表达上调,对急性脑梗死早期预防提供一定的价值,有望成为急性脑梗死患者外周血中新的生物学标志。
Objective: To discuss the expression level and clinical significance of circRNA-SRRM2 in peripheral blood of patients with acute cerebral infarction. Methods: A total of 46 patients with acute cerebral infarction less than 7 days of onset were collected as the case group, and 48 patients without cerebral infarction were collected as the control group. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of circRNA-SRRM2 in peripheral blood in the case group and the control group. Spearman and pearson correlation analysis were used to discuss the correlation between the target gene and acute cerebral infarction. At the same time, the general data of the two groups were collected, and Logistic regression analysis was used to explore the independent risk factors of acute cerebral infarction. Results: The expression level of circRNA-SRRM2 in peripheral blood of patients with acute cerebral infarction was significantly higher than that of the control group (P＜0.05). Gender, age, systolic blood pressure, diastolic blood pressure, history of hypertension, smoking history, drinking history and peripheral blood circRNA-SRRM2 expression level were positively correlated with the occurrence of acute cerebral infarction (P＜0.05). Logistic regression analysis showed that the independent risk factors of acute cerebral infarction were gender, age, systolic blood pressure, diastolic blood pressure, history of hypertension, smoking history, drinking history and peripheral blood circRNA-SRRM2 (P＜0.05). The area under the ROC curve of peripheral blood circRNA-SRRM2 in the diagnosis of acute cerebral infarction was 0.683 (P＜0.002), the critical value was 1.673, the sensitivity was 0.820, and the specificity was 0.470. Conclusion: The expression of circRNA-SRRM2 is up-regulated in acute cerebral infarction, which is expected to provide some value for the early prevention of acute cerebral infarction and become a new biological marker in peripheral blood of patients with acute cerebral infarction in the future.
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