目的:探究大鼠脑缺血再灌注损伤后神经功能缺损情况及行为学的变化。方法:将60只雄性Wistar大鼠随机分为Sham组、24 h组、48 h组、72 h组、7 d组、14 d组,采用大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)法构建大鼠局灶性脑缺血再灌注损伤(cerebral ischemia reperfusion injury,CIRI)模型。通过改良大鼠神经功能缺损评分(modified neurological severity score,mNSS)评估各组大鼠神经功能缺损情况,监测大鼠体重并记录其变化;TTC染色检测各组大鼠梗死面积;贴纸去除实验、旷场实验、转棒实验评估各组大鼠感觉和运动能力、情绪变化情况。结果:大鼠脑缺血再灌注后体重明显降低(P＜0.01),梗死面积增加(P＜0.01),以72 h最为显著;与Sham组比较,脑缺血再灌注后大鼠神经功能缺损评分升高(P＜0.01),贴纸去除时间增加(P＜0.01),并在48～72 h达高峰。旷场实验结果表明MCAO模型大鼠运动路程减少(P＜0.01),静止时间增加(P＜0.01),模型大鼠平均速度和站立次数均减少(P＜0.01),出现了抑郁情绪,对外界的好奇心和探索欲望减退。转棒实验结果显示MCAO模型大鼠运动时间和运动距离减少(P＜0.01),且均在48～72 h变化更为显著。结论:脑缺血再灌注损伤48～72 h对大鼠神经功能、运动能力减弱,肢体协调性及情绪影响最为严重。
Objective: To explore the neurological deficit and behavioral changes of rats after cerebral ischemia-reperfusion injury. Methods: A total of 60 male Wistar rats were randomly divided into the Sham group, 24 h group, 48 h group, 72 h group, 7 d group and 14 d group, and the rat model of focal cerebral ischemia reperfusion injury (CIRI) was established using middle cerebral artery occlusion (MCAO). The neurological deficit of rats in each group was evaluated by modified neurological severity score (mNSS), and the weight of rats was monitored and recorded. The rate of cerebral infarction was detected by TTC staining, the sensory and motor abilities and emotional changes of rats in each group were evaluated by Adhesive Removal Test, Open Field Test and Rotarod Test. Results: After cerebral ischemia/reperfusion, the weight of rats decreased significantly (P＜0.01), especially at 72 h; Compared with the Sham group, the neurological deficit score of rats after cerebral ischemia-reperfusion increased significantly (P＜0.01), and the removal time of adhesive tape increased significantly (P＜0.01), reaching the peak at 48～72 h. The results of open field test showed that the moving distance of rats of the MCAO model was significantly decreased (P＜0.01), while the rest time was significantly increased (P＜0.01), with the significantly decreased average speed and standing time (P＜0.01), and rats of MCAO model were depressed, with the lowered curiosity and desire to explore the outside world. The results of rotarod test showed that the moving time and distance of rats in the MCAO model were decreased (P＜0.01), and the changes were more significant from 48～72 h. Conclusion: Cerebral ischemia-reperfusion injury after 48～72 h could weaken the neurological function and motor ability of rats, which seriously affect limb coordination and emotion of rats.
[1] 王陇德, 彭斌, 张鸿祺, 等. 《中国脑卒中防治报告2020》概要[J]. 中国脑血管病杂志, 2022, 19(2): 136-144.
[2] Zhang AP, Zhang YY, Liu AF, et al. Molecular mechanism of long-term neuroprotective effects of gradual flow restoration on cerebral ischemia reperfusion injury in MCAO rats[J]. J Stroke Cerebrovasc Dis, 2020, 29(9): 105041.
[3] Katan M, Luft A. Global burden of stroke[J]. Semin Neurol, 2018, 38(2): 208-211.
[4] Lee RHC, Lee MHH, Wu CYC, et al. Cerebral ischemia and neuroregeneration[J]. Neural Regen Res, 2018, 13(3): 373-385.
[5] Lu CX, Wang CY, Xiao HX, et al. Ethyl pyruvate: a newly discovered compound against ischemia-reperfusion injury in multiple organs[J]. Pharmacol Res, 2021, 171: 105757.
[6] 徐孝旺, 徐孝兴. 注射用丹参多酚酸对新发缺血性脑卒中患者血运重建后脑缺血再灌注损伤的干预作用[J]. 药物评价研究, 2023, 46(8): 1722-1728.
[7] 王婷婷, 魏清川, 马锡超, 等. 脑卒中运动功能障碍的规范化三级康复策略探讨[J]. 华西医学, 2022, 37(5): 749-756.
[8] 吕雅琳. 急、慢性大脑中动脉闭塞性病变的相关因素及侧支循环代偿研究[D]. 长春: 吉林大学, 2023.
[9] Zhang Y, Zhang Y, Jin XF, et al. The role of astragaloside IV against cerebral ischemia/reperfusion injury: suppression of apoptosis via promotion of P62-LC3-autophagy[J]. Molecules, 2019, 24(9): 1838.
[10] 康晓宇, 刘丽旭, 王文竹, 等. 普拉克索联合左旋多巴对全脑缺血再灌注损伤大鼠认知能力及线粒体功能的影响[J]. 中国康复理论与实践, 2023, 29(5): 533-540.
[11] 凌梦钰, 杨一卓, 刘帅, 等. 运动训练对脑缺血再灌注大鼠认知功能及前额皮层神经元核抗原和突触素1表达的效果[J]. 中国康复理论与实践, 2021, 27(11): 1272-1281.
[12] 杨光, 李可法, 梁建军. 氟哌噻吨美利曲辛片联合盐酸帕罗西汀治疗老年脑卒中后抑郁的效果及对神经功能的影响[J]. 黑龙江医学, 2023, 47(22): 2739-2741.
[13] 聂皎, 高俊峰, 杨锦湄, 等. 归芍八味汤对缺血性卒中后抑郁模型大鼠行为学及神经功能的影响[J]. 中医研究, 2023, 36(7): 68-72.
