目的: 分析人乳头瘤病毒16型E6蛋白(Human papillomavirus type 16 E6 protein,HPV16-E6)、细胞分化抑制因子-1(Inhibitor of intracellular differentiation-1,ID-1)及脯氨酰顺反异构酶1(Prolylcis-trans isomerase1,Pin1)在宫颈癌组织中的表达及相关性。方法: 选择2017年3月至2022年3月莆田学院附属医院收治的宫颈癌65例,所有患者均在我院进行手术切除癌组织,并留取宫颈癌组织和距离癌组织边缘2 cm以上癌旁组织。通过免疫组化法对癌组织、癌旁组织标本HPV16-E6、ID-1、Pin1阳性表达率进行检测。分析HPV16-E6、ID-1、Pin1与临床特征的关系。结果: 与癌旁组织相比,癌组织中HPV16-E6、ID-1、Pin1阳性率较高(P＜0.05)。HPV16-E6、ID-1、Pin1与肌层浸润深度、组织学分级、临床分期、淋巴结转移、肿瘤直径呈正相关(P＜0.05)。将年龄、病理类型等因素控制后,HPV16-E6、ID-1、Pin1与肌层浸润深度、组织学分级、临床分期、淋巴结转移、肿瘤直径相关(P＜0.05)。结论: HPV16-E6、ID-1、Pin1在宫颈癌患者中均呈高表达,且HPV16-E6、ID-1、Pin1与宫颈癌患者肌层浸润深度、组织学分级、临床分期、淋巴结转移、肿瘤直径相关。
Objective: To analyze the expression and correlation of human papillomavirus type 16 E6 protein (HPV16-E6), inhibitor of intracellular differentiation-1 (ID-1) and prolylcis-trans isomerase1 (Pin1) in cervical cancer. Methods: A total of 65 patients with cervical cancer admitted to the Affiliated Hospital of Putian University from March 2017 to March 2022 were selected. All patients underwent surgical resection of cancer tissues in our hospital, and cervical cancer tissues and adjacent tissues more than 2 cm from the edge of cancer tissues were retained. The positive expression rates of HPV16-E6, ID-1 and Pin1 in cancer tissues and adjacent tissues were detected by immunohistochemistry. The relationship between HPV16-E6, ID-1, Pin1 and clinical features was analyzed. Results: Compared with adjacent tissues, the positive rates of HPV16-E6, ID-1 and Pin1 in cancer tissues were higher (P＜0.05). HPV16-E6, ID-1 and Pin1 were positively correlated with depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter (P＜0.05).. After controlling for age, pathological type and other factors, HPV16-E6, ID-1 and Pin1 were correlated with depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter (P＜0.05). Conclusion: HPV16-E6, ID-1 and Pin1 are highly expressed in patients with cervical cancer, and HPV16-E6, ID-1 and Pin1 are related to the depth of myometrial invasion, histological grade, clinical stage, lymph node metastasis and tumor diameter in patients with cervical cancer.
[1] Feng CH, Mell LK, Sharabi AB, et al. Immunotherapy with radiotherapy and chemoradiotherapy for cervical cancer[J]. SeminRadiatOncol, 2020,30(4):273-280.
[2] Preci DP, Almeida A, Weiler AL, et al. Oxidative damage and antioxidants in cervical cancer[J]. Int J Gynecol Cancer, 2021,31(2):265-271.
[3] De Felice F, Giudice E, Bolomini G, et al.Pembrolizumab for advanced cervical cancer: safety and efficacy[J]. Expert Rev Anticancer Ther, 2021,21(2):221-228.
[4] 周萍,衣秀苇,孙国丽,等. 人乳头瘤病毒16型 E6通过微小RNA-504调控宫颈癌细胞增殖、侵袭和凋亡的研究[J]. 中国性科学,2022,31(5):84-88.
[5] 杨晓艳,董国玲,刘爱敏. ID-1、ILK蛋白表达与宫颈癌病理特征的关系[J]. 实用癌症杂志,2021,36(11):1764-1767.
[6] 郝立君,徐丽秀,李金秋,等. 肽基脯氨酰同分异构酶(Pin1)对子宫颈癌细胞脂质代谢的作用[J]. 昆明医科大学学报,2021,42(1):12-16.
[7] 中国抗癌协会妇科肿瘤专业委员会.子宫颈癌诊断与治疗指南(2021年版)[J]. 中国癌症杂志,2021,31(6):474-489.
[8] 龙金晶,刘星娅,徐美. TUSC3在宫颈癌组织中的表达及其对宫颈癌细胞上皮间质转化、迁移和侵袭的影响[J]. 肿瘤药学,2022,12(2):191-196.
[9] 刘亚红,刘艳庆,邱育红. 血管内皮生长因子、糖类抗原125、糖类抗原19-9在宫颈癌中的表达及临床价值[J]. 癌症进展,2022,20(7):712-715.
[10] 陈一蕊,勾朝阳,李新阳. 血清SCC-Ag、sE-cadherin、β-HCG在宫颈癌诊断中的应用价值[J]. 实用癌症杂志,2022,37(3):376-379.
[11] 刘娟,葛彩云,戴梦源,等. 宫颈癌组织HPV16-E6表达及其促进巨噬细胞代替性活化机制研究[J]. 中华肿瘤防治杂志,2021,28(20):1562-1567.
[12] 张娟,李娜娜,陈蕊,等. TSHR、HPV16 E6蛋白在老年宫颈癌组织中表达及与疾病发生发展的关系[J]. 中国老年学杂志,2021,41(5):963-966.
[13] 许月欣,经莉,闫洪超,等. HPV16/18 E6和TRIM25蛋白在不同宫颈组织中的表达及意义[J]. 医学研究杂志,2022,51(4):127-131,143.
[14] 辛玲丽,程波,孙锁柱,等. 人乳头状瘤病毒感染宫颈病变程度与E2、E6、E7基因表达水平相关性研究[J]. 诊断病理学杂志,2021,28(12):1040-1043,1047.
[15] 黄林,陈江艳. 皮肤鳞状细胞癌和基底细胞癌组织中VEGF、COX-2及ID-1的表达及临床意义[J]. 中国现代医学杂志,2020,30(21):18-23.
[16] 蔡重阳,王凯诚,赵金锋,等. 分化抑制因子1在胃癌组织中的表达及其临床意义[J]. 中国医刊,2020,55(10):1086-1088.
[17] 张晓英. 宫颈癌患者新辅助化疗前后TK1、ID-1水平变化与疗效的关系[J]. 检验医学,2020,35(2):108-111.
[18] 黄莎莎,韩峥,谭洁,等. 抑瘤素M、Id-1、Foxp3在胃癌组织中的表达及与病理特征的相关性[J]. 实用医学杂志,2021,37(9):1150-1154.
[19] 王辉,李章富,温行,等. 肽基脯氨酰顺反异构酶1对喉癌细胞系Hep-2细胞生物学行为的影响[J]. 中国医科大学学报,2021,50(8):681-689.
[20] 欧有冠,朱蕾,鲁才杰. 胃癌组织中膜转铁蛋白及肽基脯氨酰顺反异构酶的阳性表达率与临床病理参数的关系[J]. 海军医学杂志,2021,42(3):357-359.
[21] 丁爽,禹媛,崔宇琦,等. Pin1在恶性肿瘤中的研究进展[J]. 生命的化学,2021,41(10):2163-2167.
[22] 陈婷婷,任晓燕,杨棹霞,等. Ki-67、P16、Pin1在宫颈癌组织中的表达及临床意义[J]. 解放军医药杂志,2021,33(12):30-33.
