目的: 研究原发性肝细胞癌(HCC)中14-3-3β及miRNA-152的表达水平,阐明其与患者临床病理特征的关系及14-3-3β和miRNA-152两者之间的关系。方法: 选取2019-2023年包头市肿瘤医院收治的40例HCC组织及癌旁组织,RT-qPCR方法分别检测14-3-3β mRNA及miRNA-152表达量,并用免疫组织化学PS法、Western blot检测14-3-3β蛋白的表达情况,再分别分析其表达水平上的差异。结果: HCC组织中14-3-3β表达量高于癌旁正常组织,HCC组织中miRNA-152表达量低于癌旁正常组织。miRNA-152相对高表达时,14-3-3β相对低表达(P＜0.05);并且,14-3-3β及miRNA-152的表达在HCC中呈负相关(P＜0.05)。结论: 14-3-3β蛋白在HCC组织表达上调,miRNA-152在HCC组织中表达下调,在HCC中,miRNA-152可通过负调控机制降低14-3-3β表达。
Objective: To study the expression levels of 14-3-3β and miRNA-152 in primary hepatocellular carcinoma (HCC), and to clarify their relationship with clinicopathological features and the relationship between 14-3-3β and miRNA-152. Methods: Forty patients of HCC tissues and adjacent tissues admitted to Baotou Cancer Hospital from 2019 to 2023 were selected. The expression levels of 14-3-3β mRNA and miRNA-152 were detected by RT-qPCR. The expression of 14-3-3β protein was detected by immunohistochemical PS method and Western blot, and the differences in expression levels were analyzed. Results: The expression of 14-3-3β in HCC tissues was higher than that in adjacent normal tissues, and the expression of miRNA-152 in HCC tissues was lower than that in adjacent normal tissues, meanwhile, the expression of 14-3-3β was relatively low when the expression of miRNA-152 was relatively high (P＜0.05). Moreover, the expression of 14-3-3β and miRNA-152 was negatively correlated in HCC (P＜0.05). Conclusion: The expression of 14-3-3β protein is up-regulated in HCC tissues, and the expression of miRNA-152 is down-regulated in HCC tissues. In HCC, miRNA-152 can reduce the expression of 14-3-3β through negative regulation mechanism.
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