目的: 探究CCN1蛋白在食管癌中对增殖诱导配体(APRIL)和B细胞刺激因子(BAFF)所起到的作用。方法: 用质粒转染技术在细胞株KYSE150及OE-19 中过表达CCN1,Western blot验证其转染效果,同时检测APRIL和BAFF的表达情况。结果: 在KYSE150细胞中过表达CCN1后,APRIL/BAFF高表达(P<0.05);在OE19细胞中过表达CCN1后,APRIL/BAFF低表达(P<0.05)。结论: CCN1在食管鳞癌细胞KYSE150中对APRIL/BAFF为激活作用,在食管腺癌细胞OE19中对APRIL/BAFF为抑制作用。
Objective: To investigate the role of CCN1 protein in a proliferation-inducing ligand (APRIL) and B-cell activating factor (BAFF) in esophageal cancer. Methods: CCN1 was overexpressed in KYSE150 and OE-19 cell lines by plasmid transfection technique. The transfection effect was verified by Western blot, and the expression of APRIL and BAFF was detected. Results: APRIL/BAFF was highly expressed after overexpression of CCN1 in KYSE150 cells (P<0.05), and APRIL/BAFF was lowly expressed after overexpression of CCN1 in OE19 cells (P<0.05). Conclusion: CCN1 activates APRIL/BAFF in esophageal squamous cell carcinoma cell line KYSE150 and inhibits APRIL/BAFF in esophageal adenocarcinoma cell line OE19.
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