基础医学论著

CCN1对食管癌细胞APRIL/BAFF表达的影响*

  • 邢玲玲 ,
  • 刘琳 ,
  • 江振宇 ,
  • 柴建原 ,
  • 武金宝
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  • 1.内蒙古科技大学包头医学院,内蒙古包头 014040;
    2.内蒙古科技大学包头医学院第二附属医院内蒙古消化病研究所
武金宝

收稿日期: 2024-05-10

  网络出版日期: 2024-12-24

基金资助

*内蒙古自然科学基金项目(2020LH08005);内蒙古自治区高等学校科学研究项目(JZY22057,NJZY22095);包头医学院青年科技人才发展计划(YTT-QNGG2022065);内蒙古医学科学院临床需求性基础研究项目(2023GLLH0213);内蒙古自治区教育科学研究“十四五”规划课题(NGJGH2023539)

Effect of CCN1 on APRIL/BAFF expression in esophageal cancer cells

  • XING Lingling ,
  • LIU Lin ,
  • JIANG Zhenyu ,
  • CHAI Jianyuan ,
  • WU Jinbao
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  • 1. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China;
    2. Inner Mongolia Digestive Disease Research Institute, the Second Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology

Received date: 2024-05-10

  Online published: 2024-12-24

摘要

目的: 探究CCN1蛋白在食管癌中对增殖诱导配体(APRIL)和B细胞刺激因子(BAFF)所起到的作用。方法: 用质粒转染技术在细胞株KYSE150及OE-19 中过表达CCN1,Western blot验证其转染效果,同时检测APRIL和BAFF的表达情况。结果: 在KYSE150细胞中过表达CCN1后,APRIL/BAFF高表达(P<0.05);在OE19细胞中过表达CCN1后,APRIL/BAFF低表达(P<0.05)。结论: CCN1在食管鳞癌细胞KYSE150中对APRIL/BAFF为激活作用,在食管腺癌细胞OE19中对APRIL/BAFF为抑制作用。

本文引用格式

邢玲玲 , 刘琳 , 江振宇 , 柴建原 , 武金宝 . CCN1对食管癌细胞APRIL/BAFF表达的影响*[J]. 包头医学院学报, 2024 , 40(12) : 13 -17 . DOI: 10.16833/j.cnki.jbmc.2024.12.003

Abstract

Objective: To investigate the role of CCN1 protein in a proliferation-inducing ligand (APRIL) and B-cell activating factor (BAFF) in esophageal cancer. Methods: CCN1 was overexpressed in KYSE150 and OE-19 cell lines by plasmid transfection technique. The transfection effect was verified by Western blot, and the expression of APRIL and BAFF was detected. Results: APRIL/BAFF was highly expressed after overexpression of CCN1 in KYSE150 cells (P<0.05), and APRIL/BAFF was lowly expressed after overexpression of CCN1 in OE19 cells (P<0.05). Conclusion: CCN1 activates APRIL/BAFF in esophageal squamous cell carcinoma cell line KYSE150 and inhibits APRIL/BAFF in esophageal adenocarcinoma cell line OE19.

参考文献

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