CCN1对食管癌细胞APRIL/BAFF表达的影响*

邢玲玲; 刘琳; 江振宇; 柴建原; 武金宝

doi:10.16833/j.cnki.jbmc.2024.12.003

包头医学院学报 >

2024 , Vol. 40 >Issue 12: 13 - 17

DOI: https://doi.org/10.16833/j.cnki.jbmc.2024.12.003

基础医学论著

CCN1对食管癌细胞APRIL/BAFF表达的影响^*

邢玲玲 ,
刘琳 ,
江振宇 ,
柴建原 ,
武金宝

展开

1.内蒙古科技大学包头医学院,内蒙古包头 014040;
2.内蒙古科技大学包头医学院第二附属医院内蒙古消化病研究所

武金宝

收稿日期: 2024-05-10

网络出版日期: 2024-12-24

基金资助

*内蒙古自然科学基金项目(2020LH08005);内蒙古自治区高等学校科学研究项目(JZY22057,NJZY22095);包头医学院青年科技人才发展计划(YTT-QNGG2022065);内蒙古医学科学院临床需求性基础研究项目(2023GLLH0213);内蒙古自治区教育科学研究“十四五”规划课题(NGJGH2023539)

收起

Effect of CCN1 on APRIL/BAFF expression in esophageal cancer cells

XING Lingling ,
LIU Lin ,
JIANG Zhenyu ,
CHAI Jianyuan ,
WU Jinbao

Expand

1. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China;
2. Inner Mongolia Digestive Disease Research Institute, the Second Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology

Received date: 2024-05-10

Online published: 2024-12-24

Fold

摘要

目的: 探究CCN1蛋白在食管癌中对增殖诱导配体(APRIL)和B细胞刺激因子(BAFF)所起到的作用。方法: 用质粒转染技术在细胞株KYSE150及OE-19 中过表达CCN1,Western blot验证其转染效果,同时检测APRIL和BAFF的表达情况。结果: 在KYSE150细胞中过表达CCN1后,APRIL/BAFF高表达(P＜0.05);在OE19细胞中过表达CCN1后,APRIL/BAFF低表达(P＜0.05)。结论: CCN1在食管鳞癌细胞KYSE150中对APRIL/BAFF为激活作用,在食管腺癌细胞OE19中对APRIL/BAFF为抑制作用。

关键词： CCN1; 增殖诱导配体; 细胞刺激因子; 食管癌

本文引用格式

邢玲玲 , 刘琳 , 江振宇 , 柴建原 , 武金宝 . CCN1对食管癌细胞APRIL/BAFF表达的影响^*[J]. 包头医学院学报, 2024 , 40(12) : 13 -17 . DOI: 10.16833/j.cnki.jbmc.2024.12.003

Abstract

Objective: To investigate the role of CCN1 protein in a proliferation-inducing ligand (APRIL) and B-cell activating factor (BAFF) in esophageal cancer. Methods: CCN1 was overexpressed in KYSE150 and OE-19 cell lines by plasmid transfection technique. The transfection effect was verified by Western blot, and the expression of APRIL and BAFF was detected. Results: APRIL/BAFF was highly expressed after overexpression of CCN1 in KYSE150 cells (P＜0.05), and APRIL/BAFF was lowly expressed after overexpression of CCN1 in OE19 cells (P＜0.05). Conclusion: CCN1 activates APRIL/BAFF in esophageal squamous cell carcinoma cell line KYSE150 and inhibits APRIL/BAFF in esophageal adenocarcinoma cell line OE19.

Key words： CCN1; A proliferation-inducing ligand; B-cell activating factor; Esophageal cancer

参考文献

[1] Morgan E,Soerjomataram I,Rumgay H, et al.The global landscape of esophageal squamous cell carcinoma and esophageal adenocarcinoma incidence and mortality in 2020 and projections to 2040: new estimates from GLOBOCAN 2020[J].Gastroenterology,2022,163(3):649-658.e2.
[2] Chang Z, Dang T, Meng X, et al. The role of CCN1 in esophageal adenocarcinoma: what we have learned from the lab[J].Cancer Control, 2022, 29:10732748221074734.
[3] Ullah MA, Mackay F. The BAFF-APRIL system in cancer[J].Cancers (Basel),2023,15(6):1791.
[4] Mulazzani M, Huber M, Borchard S, et al. APRIL and BAFF: novel biomarkers for central nervous system lymphoma[J].J Hematol Oncol,2019,12(1):102.
[5] Alturaiki W. The roles of B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) in allergic asthma[J].Immunol Lett,2020,225:25-30.
[6] Tian S, Chen K, Xiao J, et al. APRIL and BAFF play a key role in differentiating vitreoretinal lymphoma from uveitis[J].Clin Chim Acta,2022,535:1-6.
[7] Lee L, Lim WC, Galas-Filipowicz D, et al. Limited efficacy of APRIL CAR in patients with multiple myeloma indicate challenges in the use of natural ligands for CAR T-cell therapy [J].J Immunother Cancer,2023,11(6):e006699.
[8] Chan CC, Harley ITW, Pfluger PT, et al. A BAFF/APRIL axis regulates obesogenic diet-driven weight gain[J].Nat Commun,2021,12(1):2911.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献