目的:研究血清长链非编码RNA母系表达基因8(lncRNA MEG8)表达与缺血性脑卒中患者神经功能缺损程度的相关性。方法:将本院收治的135例缺血性脑卒中患者作为缺血性脑卒中组,并根据NIHSS评分将其细分为轻度缺损组、中度缺损组、重度缺损组;另选取于本院体检的脑神经功能正常的128例体检者为对照组。收集各组基线资料,采用实时荧光定量PCR(qRT-PCR)法检测血清lncRNA MEG8表达水平;缺血性脑卒中患者不同时间点血清lncRNA MEG8表达水平与基线资料及神经功能缺损程度相关性采用Spearman及Pearson分析;采用Logistic回归进行缺血性脑卒中患者神经功能缺损程度的影响因素分析。结果:缺血性脑卒中组入院第2 d、入院第7 d、入院第14 d血清lncRNA MEG8表达水平均高于对照组(P＜0.05)。入院第14 d血清lncRNA MEG8表达水平低于入院第2 d、入院第7 d(P＜0.05)。重度缺损组血清lncRNA MEG8表达水平高于中度缺损组和轻度缺损组(P＜0.05),中度缺损组血清lncRNA MEG8表达水平高于轻度缺损组(P＜0.05)。缺血性脑卒中患者血清lncRNA MEG8表达水平与神经功能缺损程度、高血压、颈动脉粥样硬化、糖尿病、收缩压、TC、LDL-C、Hs-CRP均呈正相关(P＜0.05),与HDL-C呈负相关(P＜0.05)。lncRNA MEG8、颈动脉粥样硬化是缺血性脑卒中患者发展为中/重度神经功能缺损的独立危险因素(P＜0.05)。结论:血清lncRNA MEG8表达水平与缺血性脑卒中患者神经功能缺损程度呈正相关。
Objective: To study the correlation between the expression of serum long non-coding RNA maternally expressed gene 8 (lncRNA MEG8) and the degree of neurological deficit in patients with ischemic stroke. Methods: A total of 135 patients with ischemic stroke admitted in our hospital were selected as ischemic stroke group, and they were divided into mild defect group, moderate defect group and severe defect group according to NIHSS score. In addition, 128 subjects with normal cranial nerve function in our hospital were selected as the control group. Baseline data were collected from each group, the expression level of serum lncRNA MEG8 was detected by real-time fluorescence quantitative PCR (qRT-PCR) method; the correlation between the expression level of serum lncRNA MEG8 with baseline data and the degree of neurological deficit in patients with ischemic stroke at different time was analyzed by Spearman and Pearson analyses; Logistic regression was used to analyze the influencing factors of the degree of neurological deficit in patients with ischemic stroke. Results: The expression levels of serum lncRNA MEG8 in the ischemic stroke group on the 2nd, 7th and 14th day of admission were higher than those in the control group (P＜0.05). The expression level of serum lncRNA MEG8 on the 14th day of admission was lower than that on the 2nd and 7th day of admission (P＜0.05). The expression level of serum lncRNA MEG8 in the severe defect group was higher than that in the moderate defect group and mild defect group (P＜0.05), and the the expression level of serum lncRNA MEG8 in the moderate defect group was higher than that in the mild defect group (P＜0.05). The expression level of serum lncRNA MEG8 in patients with ischemic stroke was positively correlated with the degree of neurological deficit, hypertension, carotid atherosclerosis, diabetes, systolic blood pressure, TC, LDL-C, and Hs-CRP (P＜0.05), while was negatively correlated with HDL-C (P＜0.05). LncRNA MEG8 and carotid atherosclerosis were independent risk factors for patients with ischemic stroke to develop moderate/severe neurological deficit (P＜0.05). Conclusion: The expression level of serum lncRNA MEG8 is positively correlated with the degree of neurological deficit in ischemic stroke patients.
[1] 华键, 周其达, 秦琳, 等. 颅内动脉狭窄与急性轻型缺血性脑卒中神经功能恶化及早期预后的关系[J]. 中西医结合心脑血管病杂志, 2021, 19(11):1911-1913.
[2] Paul S, Candelario-Jalil E. Emerging neuroprotective strategies for the treatment of ischemic stroke: an overview of clinical and preclinical studies[J]. Exp Neurol, 2021,335:113518.
[3] Xing Y, Bai YL. A review of exercise-induced neuroplasticity in ischemic stroke: pathology and mechanisms[J]. Mol Neurobiol, 2020,57(10):4218-4231.
[4] Lou J, Yan W, Li QY, et al. LncRNA MEG8 plays an oncogenic role in hepatocellular carcinoma progression through miR-367-3p/14-3-3ζ/TGFβR1 axis[J]. Neoplasma, 2021,68(2):273-282.
[5] Zheng YH, Sun SP, Yu M, et al. Identification of potential hub-lncRNAs in ischemic stroke based on Subpathway-LNCE method[J]. J Cell Biochem, 2019,120(8):12832-12842.
[6] 中华医学会神经病学分会, 中华医学会神经病学分会脑血管病学组, 彭斌, 等. 中国急性缺血性脑卒中诊治指南2018[J]. 中华神经科杂志, 2018, 51(9):666-682.
[7] Eskioglou E, Huchmandzadeh Millotte M, Amiguet M, et al. National institutes of health stroke scale zero strokes[J]. Stroke, 2018,49(12):3057-3059.
[8] Carandina A, Lazzeri G, Villa D, et al. Targeting the autonomic nervous system for risk stratification, outcome prediction and neuromodulation in ischemic stroke[J]. Int J Mol Sci, 2021,22(5):2357.
[9] Shkirkova K, Saver JL, Starkman S, et al. Frequency, predictors, and outcomes of prehospital and early postarrival neurological deterioration in acute stroke: exploratory analysis of the FAST-MAG randomized clinical trial[J]. JAMA Neurol, 2018,75(11):1364-1374.
[10] Gong PY, Liu YK, Gong YC, et al. The association of neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, and lymphocyte to monocyte ratio with post-thrombolysis early neurological outcomes in patients with acute ischemic stroke[J]. J Neuroinflammation, 2021,18(1):51.
[11] 郑晶, 杨斐斐, 占茂芳, 等. 急性缺血性脑卒中患者静脉溶栓后早期神经功能恶化的研究[J]. 国际神经病学神经外科学杂志, 2020, 47(3):272-276.
[12] Cai HY, Huang HH, Yang CG, et al. Eosinophil-to-neutrophil ratio predicts poor prognosis of acute ischemic stroke patients treated with intravenous thrombolysis[J]. Front Neurol, 2021,12:665827.
[13] 李俊杰, 邵建林. 长链非编码RNAs-微小RNAs-mRNAs调控轴在脑缺血/再灌注损伤机制中的作用进展[J]. 实用医学杂志, 2021, 37(12):1631-1635.
[14] Vasudeva K, Dutta A, Munshi A. Role of lncRNAs in the development of ischemic stroke and their therapeutic potential[J]. Mol Neurobiol, 2021,58(8):3712-3728.
[15] Xiang YX, Zhang YY, Xia YN, et al. LncRNA MEG3 targeting miR-424-5p via MAPK signaling pathway mediates neuronal apoptosis in ischemic stroke[J]. Aging, 2020,12(4):3156-3174.
[16] Sui SH, Sun L, Zhang WJ, et al. LncRNA MEG8 attenuates cerebral ischemia after ischemic stroke through targeting miR-130a-5p/VEGFA signaling[J]. Cell Mol Neurobiol, 2021,41(6):1311-1324.
[17] 关晶波, 袁蓉, 丛伟红. 颈动脉粥样硬化中医证候分布及心脑血管疾病危险因素分析[J]. 中国中西医结合杂志, 2020, 40(3):295-299.
[18] Zhang BY, Dong Y, Zhao Z. LncRNA MEG8 regulates vascular smooth muscle cell proliferation, migration and apoptosis by targeting PPARα[J]. Biochem Biophys Res Commun, 2019,510(1):171-176.
[19] Qin Z, Tang LS, Huang QY, et al. A systematic review of the correlation between serum asymmetric dimethylarginine, carotid atherosclerosis and ischaemic stroke[J]. Eur J Clin Invest, 2021,51(8):e13558.
[20] Sharma D, Gandhi N. Role of platelet to lymphocyte ratio (PLR) and its correlation with NIHSS (national institute of health stroke scale) for prediction of severity in patients of acute ischemic stroke[J]. J Assoc Physicians India, 2021,69(1):56-60.
[21] Ospel JM, Brown S, Kappelhof M, et al. Comparing the prognostic impact of age and baseline national institutes of health stroke scale in acute stroke due to large vessel occlusion[J]. Stroke, 2021,52(9):2839-2845.
