目的:探究miR-152-3p、DNA甲基转移酶1(DNA methyltransferases, DNMT1)在结肠癌细胞中的调控机制。方法:qRT-PCR检测miR-152-3p和DNMT1的表达;细胞增殖实验、克隆形成实验、划痕愈合实验以及Transwell实验检测各处理组癌细胞的增殖、迁移和侵袭能力;双荧光素酶实验检测miR-152-3p与DNMT1的靶向关系;Western blot实验检测DNMT1蛋白表达;流式细胞术实验检测细胞凋亡率。结果:结肠癌细胞中miR-152-3p显著低表达,DNMT1显著高表达;过表达miR-152-3p会抑制结肠癌细胞增殖、迁移和侵袭;miR-152-3p能靶向抑制DNMT1的表达,从而抑制结肠癌细胞的增殖、迁移和侵袭;过表达miR-152-3p可以靶向DNMT1并促进细胞凋亡。结论:miR-152-3p通过靶向下调DNMT1的表达,从而抑制结肠癌细胞的发生发展。
Objective: To investigate the regulatory mechanism of miR-152-3p and DNA Methyltransferases(DNMT1) in colon cancer cells. Methods: qRT-PCR was used to detect the expression of miR-152-3p and DNMT1. Cell proliferation assay, colony formation assay, scratch healing assay and Transwell assay were used to detect the proliferation, migration and invasion ability of cancer cells in each treatment group. Dual luciferase assay was used to detect the targeting relationship between miR-152-3p and DNMT1. Western blot assay was used to detect the protein expression of DNMT1. Flow cytometry assay was used to detect apoptosis rate of cells. Results: MiR-152-3p was significantly downregulated while DNMT1 was highly expressed in colon cancer cells. Overexpression of miR-152-3p inhibited the proliferation, migration and invasion of colon cancer cells. MiR-152-3p could inhibit the expression of DNMT1, thereby inhibiting the proliferation, migration and invasion of colon cancer cells. Overexpression of miR-152-3p could target DNMT1 and promote cell apoptosis. Conclusion: MiR-152-3p could downregulate the expression of DNMT1, thereby inhibiting the development of colon cancer cells.
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