目的:利用生物信息学方法,探讨脂酰转移酶1(lipoyltransferase 1, LIPT1)在乳腺癌发生发展及免疫浸润中的作用。方法:运用TCGA、GEPIA、HPA、UALCAN、Kaplan-Meier、TIMER 2.0等数据库,分析LIPT1在乳腺癌中的表达情况、蛋白互相作用网络及对乳腺浸润性癌免疫浸润的影响。结果:LIPT1表达与乳腺浸润性癌相关,PPI网络分析LIPT1蛋白表达与酰基辅酶A合成酶中链家族成员1、二氢硫辛胺支链转酰基酶、二氢硫辛酸转乙酰基酶、二硫辛酰胺转乙酰酶、二氢硫辛酸脱氢酶、甘氨酸裂解系统蛋白H、脂酰合酶、脂酰转移酶2、丙酮酸脱氢酶β以及丙酮酸脱氢酶复合体成分X相关;乳腺浸润性癌中LIPT1表达与CD8+T细胞、CD4+T细胞、巨噬细胞以及中性粒细胞浸润相关(P＜0.001)。结论:生物信息学预测LIPT1在乳腺浸润性癌中表达下调,并与肿瘤免疫细胞浸润相关。
Objective: To explore the role of lipoyltransferase (LIPT1) in the development and immune infiltration of breast cancer by bioinformatics. Methods: TCGA, GEPIA, HPA, UALCAN, Kaplan-Meier, TIMER 2.0 and other databases were used to explore the differential expression, function and related mechanism of LIPT1 in breast cancer, and to analyze the effect of LIPT1 on the immune infiltration of invasive breast cancer. Results: The expression of LIPT1 was associated with invasive breast cancer, and PPI network analysis showed that LIPT1 was associated with ACSM1, DBT, DLAT, DLT, DLST, GCSH, LIAS, LIPT2, PDHB, and PDHX; the expression of LIPT1 was associated with immune cell infiltration in invasive breast cancer. Conclusion: Bioinformatics predicts that LIPT1 expression is down-regulated in invasive breast cancer and is associated with tumor immune cell infiltration.
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