目的: 运用网络药理学的方法,探讨蒙药森登-9汤(布病Ⅱ号)治疗布鲁氏菌病的作用机制。方法: 运用BATMAN-TCM数据库分析蒙药森登-9汤的活性成分及潜在靶点;通过Genecards、OMIM、DisGeNET数据库筛选布鲁氏菌病相关靶点;构建韦恩图,得到疾病交集靶点与蒙药森登-9汤治疗布鲁氏菌病的交集靶点;用 Cytoscape 软件绘制“药物-成分－疾病－靶点”关系网络图;通过STRING 数据库构建“活性成分-疾病”共同靶点的蛋白互作(PPI)网络;使用David 数据库对关键靶标进行基因本体功能(GO)及京都基因和基因组百科全书(KEGG)通路富集分析。结果: 蒙药森登-9汤活性成分有182个,作用于 1 306 个靶点;布鲁氏菌病靶点620个,药物与疾病交集靶点152个。蒙药森登-9汤治疗布鲁氏菌病的主要活性成分有月桂酸、肉豆蔻酸、亚油酸、茉莉酮、羽扇烯酮、香叶基丙酮、氧化槐果碱、3-壬烯-2-酮等;治疗布鲁氏菌病的核心靶点有IL-6、IL-1β、INS、TNF、PTGS2、JUN、TP53、IGF1、CCL2、HIF1A。富集得到GO条目1 064个, 其中BP条目834个, CC条目107个, MF条目123 个。KEGG信号通路共162条,主要集中在HIF-1信号通路、TNF信号通路、MAPK 信号通路、FoxO信号通路、IL-17信号通路等。结论: 本研究通过网络药理学的方法初步探讨了蒙药森登-9汤有效成分抗炎、抗病原微生物等作用机制,为布鲁氏菌病的治疗,及进一步研究该方作用机理、临床应用提供了理论依据。
Objective: To explore the mechanism of Mongolian medicine Sendeng-9 Decoction (Brucellosis Ⅱ) in the treatment of brucellosis by network pharmacology. Methods: The BATMAN-TCM database was used to analyze the active components and potential targets of Mongolian medicine Sendeng-9 decoction. Brucellosis-related targets were screened through Genecards, OMIM, and DisGeNET databases. The Venn diagram was constructed to obtain the intersection target of disease and the intersection target of Mongolian medicine Sendeng-9 decoction in the treatment of brucellosis. The network diagram of “drug-component-disease-target” relationship was drawn by Cytoscape software. The protein-protein interaction (PPI) network of ' active ingredient-disease ' common targets was constructed by STRING database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of key targets were performed using the David database. Results: There were 182 active components of Mongolian medicine Sendeng-9 Decoction, which acted on 1306 targets. There were 620 brucellosis targets and 152 drug-disease intersection targets. The main active ingredients of Mongolian medicine Sendeng-9 Decoction in the treatment of brucellosis were lauric acid, myristic acid, linoleic acid, jasmonone, lupinone, geranylacetone, oxysophocarpine, 3-nonen-2-one, etc. The core targets for the treatment of brucellosis were IL-6, IL-1β, INS, TNF, PTGS2, JUN, TP53, IGF1, CCL2, HIF1A. A total of 1064 GO entries were obtained, including 834 BP entries, 107 CC entries, and 123 MF entries. There were 162 KEGG signaling pathways, mainly focusing on HIF-1 signaling pathway, TNF signaling pathway, MAPK signaling pathway, FoxO signaling pathway, IL-17 signaling pathway and so on. Conclusion: Through the method of network pharmacology, this study preliminarily discusses the anti-inflammatory and disease-resistant pathogenic microorganisms of the effective components of Mongolian medicine Senden-9 decoction, which provides a theoretical basis for the treatment of brucellosis, and further study of the mechanism of action and clinical application of this prescription.
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