目的: 通过检测急性心肌梗死患者、健康人群血浆中miR-296-5p、miR-107表达水平及在急性心肌梗死患者体内时间变化趋势,探讨其作为急性心肌梗死诊断标志物的可能性。方法: 选取2021年11月—2022年3月间在包头市中心医院心内科就诊急性心肌梗死患者60例(心梗组)和同时期在体检中心体检的年龄相匹配的健康就诊者60例(对照组)。心梗组根据采取干预措施不同分为再灌注组30例,常规药物治疗30例。应用荧光定量实时PCR技术(RT-qPCR)检测入院时及采取干预措施后不同时间段血浆miR-296-5p、miR-107的相对表达量。结果: 与对照组相比,急性心肌梗死患者血浆miR-296-5p、miR-107表达量显著升高(P＜0.05)。经再灌注治疗或常规药物治疗48 h后,血浆miR-296-5p、miR-107相对表达量明显降低(P＜0.05)。经再灌注治疗或常规药物治疗7 d后,血浆miR-296-5p、miR-107相对表达量降至正常人水平(P＜0.05)。结论: 急性心肌梗死患者血浆miR-296-5p、miR-107相对表达含量较正常人显著升高,在采取干预措施后呈下降趋势,对急性心肌梗死的诊断有预测价值。
Objective: To explore the possibility of miR-296-5p and miR-107 as diagnostic markers of acute myocardial infarction by detecting the expression levels of miR-296-5p and miR-107 in plasma of patients with acute myocardial infarction and healthy people and the time trend in patients with acute myocardial infarction. Methods: From November 2021 to March 2022, 60 patients with acute myocardial infarction (myocardial infarction group) and 60 age-matched healthy patients (control group) who underwent physical examination in the physical examination center of Baotou Central Hospital were selected as the research objects. The myocardial infarction group was divided into reperfusion group (30 patients) and conventional drug treatment group (30 patients) according to different intervention measures. Fluorescence quantitative real-time PCR (RT-qPCR) was used to detect the relative expression of plasma miR-296-5p and miR-107 at admission and at different time points after intervention. Results: Compared with the control group, the expression levels of plasma miR-296-5p and miR-107 in patients with acute myocardial infarction were significantly increased (P＜0.05). After 48 hours of reperfusion therapy or conventional drug treatment, the relative expression levels of plasma miR-296-5p and miR-107 were significantly decreased (P＜0.05). After 7 days of reperfusion therapy or conventional drug treatment, the relative expression of plasma miR-296-5p and miR-107 decreased to normal levels (P＜0.05). Conclusion: The relative expression levels of miR-296-5p and miR-107 in plasma of patients with acute myocardial infarction are significantly higher than those of normal individuals, and shows a downward trend after intervention measures, which has predictive value for the diagnosis of acute myocardial infarction.
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