目的:观察蒙药清心沉香八味散的抗抑郁作用及对单胺类神经递质的影响。方法:将实验动物(雄性昆明种小鼠)随机分为5组,分别为空白对照组(蒸馏水)、阳性药对照组(盐酸氟西汀0.02 g/kg)及受试蒙药清心沉香八味散高、中、低剂量组(27、9、3 g/kg),各组小鼠采用灌胃方式给药,7 d后采用小鼠悬尾实验(TST)、小鼠强迫游泳实验(FST)初步评估其抗抑郁作用。FST行为学实验完成后取血浆及海马组织,采用酶联免疫吸附方法(ELISA)分别检测动物血浆及海马组织中5-HT、DA、NE的含量。结果:与空白组比较,蒙药中、低剂量组能显著缩短小鼠FST的行为绝望时间,差异具有统计学意义(P＜0.05),蒙药低剂量组能显著缩短小鼠TST的行为绝望时间,差异具有统计学意义(P＜0.05),蒙药清心沉香八味散低剂量组能显著升高小鼠血浆中DA水平,差异具有统计学意义(P＜0.05),蒙药清心沉香八味散低、中、高剂量组能显著升高小鼠海马组织中5-HT、DA、NE的含量,差异具有统计学意义(P＜0.05)。结论:蒙药清心沉香八味散对小鼠可能有一定的抗抑郁样作用,其作用可能与其调节单胺类神经递质的含量相关。
Objective: To investigate the antidepressive effect of Qingxin Chenxiang Bawei Powder and its effect on monoamine neurotransmitters.Methods: Kunming male mice were randomly divided into the blank control group (distilled water), positive-drug group (fluoxetine hydrochloride 0.02 g/kg) and Mongolian medicine Qingxin Chenxiang Bawei Powder groups [the high-dose (27 g/kg) group, medium-dose (9 g/kg)group and low-dose (3 g/kg) group].Intragastric administration were performed on all experimental mice.Mice tail suspension test (TST) and forced swimming test (FST) were used to evaluate the antidepressive effect of the drug 7 days after intragastric administration.After FST behavioral experiment, the contents of 5-HT, DA and NE in plasma and hippocampus were determined by enzyme-linked immunosorbent assay (ELISA).Results: Compared with the blank control group, medium-dose and low-dose groups of Mongolian medicine could significantly shorten the immobility time in mice FST, and the difference was statistically significant (P＜0.05).The immobility time in mice TST was significantly shortened in the low-dose group, and the difference was statistically significant(P＜0.05).The plasma DA level was significantly increased in the low-dose group, and the difference was statistically significant (P＜0.05).The levels of 5-HT, DA and NE in the hippocampus of mice in the low-dose, medium-dose and high-dose groups were significantly increased, and the difference was statistically significant (P＜0.05).Conclusion: Qingxin Chenxiang Bawei Powder have certain antidepressive effect on mice, which may be related to its regulation effect on monoamine neurotransmitter content.
[1] 陈颖, 袁勇贵.中药单体、药对、复方、中成药治疗抑郁症研究进展[J].中国临床药理学与治疗学, 2021, 26(5): 586-593.
[2] 周苗苗, 魏盛, 冯雪研, 等.中药强志胶囊对抑郁模型大鼠神经行为及不同脑区单胺类神经递质的影响[J].时珍国医国药, 2020, 31(11): 2565-2568.
[3] 于冰清, 邵欣欣, 付晓凡, 等.抗抑郁中药复方的组方特点及作用机制研究[J].中草药, 2021, 52(11): 3344-3352.
[4] 王婷, 孙静莹, 王云, 等.近10年天然药物抗抑郁研究进展[J].广东药科大学学报, 2019, 35(6): 853-857.
[5] 白荣, 苏和.蒙药槟榔十三味丸抗抑郁作用和改善抑郁综合症状[J].世界最新医学信息文摘, 2017, 17(55): 21-22.
[6] 任小梅, 任常胜.抑郁症蒙医治疗概述[J].中国民族医药杂志, 2015, 21(7): 50-52.
[7] Steru L, Chermat R, Thierry B, et al.The tail suspension test: a new method for screening antidepressants in mice[J].Psychopharmacology, 1985, 85(3): 367-370.
[8] Porsolt RD, Bertin A, Jalfre M.Behavioral despair in mice: a primary screening test for antidepressants[J].Arch Int Pharmacodyn Ther, 1977, 229(2): 327-336.
[9] 毕力格, 满达, 白音夫.八味清心沉香胶囊与散剂对动物心血管与镇静作用的对比研究[J].中国民族医药杂志, 2007, 13(3): 64-65.
[10] 薛涛, 邬丽莎, 刘新民, 等.抑郁症动物模型及评价方法研究进展[J].中国实验动物学报, 2015, 23(3): 321-326.
[11] 温瀑, 李玲玲, 牛源菲, 等.细辛干预小鼠抑郁的初步研究[J].世界中医药, 2018, 13(4): 794-798.
[12] Chatterjee M, Verma P, Maurya R, et al.Evaluation of ethanol leaf extract of Ocimum sanctum in experimental models of anxiety and depression[J].Pharm Biol, 2011, 49(5): 477-483.
[13] 潘开瑞, 崔琳, 张振巍, 等.远志酊对小鼠脑神经递质及氧化应激作用的研究[J].中医研究, 2017, 30(5): 69-72.
[14] 李春艳, 王宇红, 王华, 等.抑郁合并失眠对大鼠HPA轴及下丘脑中氨基酸类和单胺类神经递质的影响[J].中国药理学通报, 2021, 37(6): 815-822.
[15] 王永杰, 刘广超, 王慧娟, 等.血府逐瘀汤对抑郁模型小鼠行为学及脑内单胺类神经递质的影响[J].山西中医药大学学报, 2021, 22(2): 97-100.
[16] Zhou XJ, Liu M, Yan JJ, et al.Antidepressant-like effect of the extracted of Kai Xin San, a traditional Chinese herbal prescription, is explained by modulation of the central monoaminergic neurotransmitter system in mouse[J].J Ethnopharmacol, 2012, 139(2): 422-428.
[17] Troubat R, Barone P, Leman S, et al.Neuroinflammation and depression: a review[J].Eur J Neurosci, 2021, 53(1): 151-171.
[18] Elhwuegi AS.Central monoamines and their role in major depression[J].Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2004, 28(3): 435-451.
[19] 高贵元, 黄捷, 刘丹, 等.抑郁症的发病机制及抗抑郁药物的研究进展[J].中国医药导报, 2021, 18(1): 52-55, 70.
