目的:探讨大鼠脑出血后注射人参皂苷Rg1促进功能恢复机制中有无凋亡相关因子的参与。方法:大鼠随机分为3组,对照组大鼠脑立体定位下于内囊内侧注射生理盐水1 μL,模型组注射Ⅶ型胶原酶1 μL,术后1 d、7 d、14 d进行大鼠运动功能评分;人参皂苷组于造模后次日腹腔注射人参皂苷Rg1;7 d后测定大鼠脑含水量,脑组织进行免疫组化观察Bcl-2、Bax的表达。结果:脑出血模型组内囊周围神经细胞有明显细胞形态受损,细胞边界不清,结构移位受压;在1 d、7 d、14 d时,模型组大鼠神经功能评分高于对照组(P<0.05),人参皂苷Rg1组大鼠在7 d、14 d时低于模型组(P<0.05);7 d时脑含水量测定模型组高于对照组(P<0.05),人参皂苷Rg1组低于模型组(P<0.05);模型组和人参皂苷Rg1组大鼠内囊区周围的Bax阳性神经元数量高于对照组(P<0.05);Bcl-2阳性细胞数量在模型组大鼠脑切片中低于对照组,人参皂苷Rg1组高于模型组,低于对照组(P<0.05)。结论:大鼠脑出血后注射人参皂苷Rg1,有利于大鼠运动功能恢复;相关脑区的Bcl-2阳性细胞增加。
Objective: To analyze the participation of apoptosis-related factors in the mechanism of ginsenoside Rg1 promoting functional recovery after intracerebral hemorrhage in rats. Methods: The rats were randomly divided into three groups, while the rats in the control group were injected with 1 μL of normal saline into the inner capsule under stereotaxic localization, and the rats in the model group were injected with 1 μL of type Ⅶ collagenase. The motor function of the rats was scored on 1st, 7th and 14th day after operation. The ginsenoside group was intraperitoneally injected with ginsenoside Rg1 on the next day after modeling. The brain water content of rats was measured on the 7th day after operation, and the expression of Bcl-2 and Bax in brain tissue was observed by immunohistochemistry. Results: In the model group of intracerebral hemorrhage, there were significant cell morphology damage, unclear cell boundaries, and structural displacement and compression of the nerve cells around the inner capsule; On the 1st, 7th and 14th day, the neurological function score of the model group was higher than that of the control group (P<0.05), and that in the ginsenoside Rg1 group was lower than that in the model group on the 7th and 14th day (P<0.05); On the 7th day, the brain water content in the model group was higher than that in the control group (P<0.05), and that in the ginsenoside Rg1 group was lower than that in the model group (P<0.05); The number of Bax positive neurons around the inner capsule area in the model group and ginsenoside Rg1 group was more than that in the control group (P<0.05); The number of Bcl-2 positive cells in brain slices of rats in the model group was lower than that in the control group, and that in the ginsenoside Rg1 group was higher than that in the model group and lower than that in the control group (P<0.05). Conclusion: The injection of ginsenoside Rg1 after intracerebral hemorrhage in rats is beneficial to the recovery of motor function, and the increase of Bcl-2 positive cells in related brain regions.
[1] 苏男,石素琴,高天一,等. 脑出血实验动物模型动物选择与制备方法的研究现状[J]. 包头医学院学报,2022,38(12):91-96.
[2] 冯乘铭,寇珍珍,周红英,等. 大鼠丘脑出血后丘脑网状核中GAD67和MOR受体含量的变化[J]. 包头医学院学报,2022,38(11):34-38.
[3] 魏山山,王孟迪,姜宁,等. 人参皂苷Rg1、Rb1、Rg1+Rb1改善东莨菪碱致小鼠认知障碍的作用比较[J]. 中国比较医学杂志,2022,32(4):94-101.
[4] 索胧胧,蔡志平,杨占君,等. 人参皂甙Rg1对大鼠坐骨神经损伤后修复的剂量效应[J]. 包头医学院学报,2016,32(9):4-5.
[5] 李秋畅,闫顺昌,蒙亚珍,等. Nrf2-GPX4介导的铁死亡通路参与右美托咪定对脑出血大鼠神经保护作用的机制研究[J]. 天津医药,2022,50(8):817-821.
[6] 杨智鑫,张振兴,李晨光,等. 神经内镜与显微镜在治疗高血压性丘脑出血破入脑室的效果对比[J]. 锦州医科大学学报,2022,43(5):59-63.