目的:探讨骨肉瘤细胞中LncRNA-ANCR的表达情况以及LncRNA-ANCR对骨肉瘤细胞生物学的影响。方法:采用荧光定量PCR检测实验各组细胞中LncRNA-ANCR的表达水平,采用CCK-8法检测各组细胞的增殖率,采用流式细胞术检测细胞凋亡,采用Transwell小室法检测细胞的侵袭、迁移能力。结果:MG-63和UMR-106骨肉瘤细胞LncRNA-ANCR的表达水平最高,选择其进行后续实验研究;与WT组相比较,Mock组、NC组细胞中LncRNA-ANCR的表达水平、转染后细胞的增殖率、细胞凋亡率、侵袭细胞和迁移数均无明显变化(P＞0.05);与WT组相比较,siRNA组细胞中LncRNA-ANCR的表达水平、转染后增殖率、细胞的侵袭和迁移数显著下降(P＜0.05),细胞的凋亡率明显上升(P＜0.05)。结论:LncRNA-ANCR可促进MG-63和UMR-106骨肉瘤细胞的增殖、侵袭以及转移,抑制细胞的凋亡。
Objective: To investigate the expression of LncRNA-ANCR in osteosarcoma cells and the effect of LncRNA-ANCR on the biology of osteosarcoma cells. Methods: The expression level of LncRNA-ANCR in each group was detecteol by fluorescence quantitative PCR. The proliferation rate of each group was detected by CCK-8 method. Flow cytometry was used to detect apoptosis. Transwell chamber method was used to detect cell invasion and migration ability. Results: The expression level of LncRNA-ANCR in MG-63 and UMR-106 osteosarcoma cells was the highest, and it was selected for subsequent experimental research. Compared with the WT group, there was no significant change in the expression level of LncRNA-ANCR, cell proliferation rate, apoptosis rate, invasive cells and migration number in Mock group and NC group (P＞0.05). Compared with the WT group, the expression level of LncRNA-ANCR, the proliferation rate after transfection, the number of invasion and migration of cells decreased significantly (P＜0.05), and the apoptosis rate of cells increased significantly in the siRNA group (P＜0.05). Conclusion: LncRNA-ANCR can promote the proliferation, invasion and metastasis of MG-63 and UMR-106 osteosarcoma cells, and inhibit cell apoptosis.
[1] Chen Y, Tang GQ, Qian HB, et al. LncRNA LOC100129620 promotes osteosarcoma progression through regulating CDK6 expression, tumor angiogenesis, and macrophage polarization[J]. Aging, 2021,13(10):14258-14276.
[2] Tong CJ, Deng QC, Ou DJ, et al. LncRNA RUSC1-AS1 promotes osteosarcoma progression through regulating the miR-340-5p and PI3K/AKT pathway[J]. Aging, 2021,13(16):20116-20130.
[3] Ma WG, Zhao X, Xue N, et al. The LINC01410/miR-122-5p/NDRG3 axis is involved in the proliferation and migration of osteosarcoma cells[J]. IUBMB Life, 2021,73(4):705-717.
[4] Wang Y, Wang YY, Qin ZY, et al. The role of non-coding RNAs in ABC transporters regulation and their clinical implications of multidrug resistance in cancer[J]. Expert Opin Drug Metab Toxicol, 2021,17(3):291-306.
[5] Wang Q, Liu MJ, Bu J, et al. miR-485-3p regulated by MALAT1 inhibits osteosarcoma glycolysis and metastasis by directly suppressing c-MET and AKT3/mTOR signalling[J]. Life Sci, 2021,268:118925.
[6] Li HQ, Zhao J, Jia XM, et al. miR-21 promotes growth, invasion and migration of lung cancer cells by AKT/P-AKT/cleaved-caspase 3/MMP-2/MMP-9 signaling pathway[J]. Int J Clin Exp Pathol, 2020,13(4):692-700.
[7] Kang HR, Moon JY, Ediriweera MK, et al. Dietary flavonoid myricetin inhibits invasion and migration of radioresistant lung cancer cells (A549-IR) by suppressing MMP-2 and MMP-9 expressions through inhibition of the FAK-ERK signaling pathway[J]. Food Sci Nutr, 2020,8(4):2059-2067.
[8] BenAyed-Guerfali D, Dabbèche-BourichaE, Ayadi W, et al. Association of FOXA1 and EMT markers (Twist1 and E-cadherin) in breast cancer[J]. Mol Biol Rep, 2019,46(3):3247-3255.
[9] Yuan JH, Yang F, Wang F, et al. A long noncoding RNA activated by TGF-β promotes the invasion-metastasis cascade in hepatocellular carcinoma[J]. Cancer Cell, 2014,25(5):666-681.
[10] Peng Y, Guo JJ, Liu YM, et al. microRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression[J]. Biosci Rep, 2014,34(3):e00112.
[11] Wang T, Gilkes DM, Takano N, et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis[J]. Proc Natl Acad Sci USA, 2014,111(31):E3234-E3242.
[12] Yang ZY, Yang F, Zhang YL, et al. LncRNA-ANCR down-regulation suppresses invasion and migration of colorectal cancer cells by regulating EZH2 expression[J]. Cancer Biomark, 2017,18(1):95-104.
[13] Li ZW, Hou PF, Fan DM, et al. The degradation of EZH2 mediated by lncRNA ANCR attenuated the invasion and metastasis of breast cancer[J]. Cell Death Differ, 2017,24(1):59-71.
