目的:探讨TLR3基因 SNP rs3775290与慢性乙型肝炎病毒感染性风险的关系。方法:应用病例对照研究方法,在内蒙古鄂尔多斯市选取172例慢性乙型肝炎患者(作为CHB病例组)和200例健康者(作为对照组)。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法检测CHB病例组和对照组TLR3基因SNP rs3775290 位点的基因多态性。并用非条件性Logisic回归计算比值比(OR)及其95 %可信区间(CI)以评估等位基因、基因型与慢性乙肝发病风险的关系。结果:SNP rs3775290位点在对照组中GG、AG 、AG基因型的频率分别为50.5 %、39.5 %、10.0 %, CHB病例组GG、AG、AA基因型的频率分别为56.4 %、34.9 %、8.7 %,两组差异无统计学意义(P＞0.05)。rs3775290位点在共显性遗传模型、显性遗传模型、隐性遗传模型、超显性遗传模型下均与慢性HBV感染风险无关(P＞0.05)。结论:TLR3 rs3775290与内蒙古鄂尔多斯市汉族人群慢性乙型肝炎病毒感染无风险关联。

Objective: To investigate the relationship between Toll-Like Receptor 3 (TLR3) rs3775290 and the risk of chronic hepatitis B virus infection. Methods: A total of 172 patients with chronic hepatitis B were selected as the CHB case group and 200 healthy people as the control group in Ordos, Inner Mongolia. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used to detect the gene polymorphism of TLR3 rs3775290 in the CHB case group and the control group. The odds ratio (OR) and its 95 % confidence interval (CI) were calculated by non-conditional Logistic regression to assess the relationship between alleles,genotypes and the risk of chronic hepatitis B infection. Results: Frequencies of SNP rs3775290 on GG, AG and AG genotypes in the control group were 50.5 %, 39.5 % and 10.0 % respectively, while the frequencies in the CHB case group were 56.4 %, 34.9 % and 8.7 % respectively. There was no significant difference between the two groups (P＞0.05). SNP rs3775290 was not associated with the risk of chronic HBV infection under codominant genetic model , dominant genetic model, recessive genetic model and super dominant genetic model (P＞0.05). Conclusion: TLR3 rs3775290 has no relationship with the risk of chronic hepatitis B virus infection in Han population in Ordos, Inner Mongolia.