目的:建立反相高效液相色谱(reversed-phase high performance liquid chromatogra,RP-HPLC)法测定香青兰乙酸乙酯提取物中木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和田蓟苷的含量。方法:采用Inertsil ODS-SP(4.6×150 nm,5 μm)色谱柱,以乙腈(A)-0.1 %甲酸水溶液(B)为流动相,梯度洗脱,流速1 mL/min,检测波长254 nm,柱温35 ℃,进样体积10 μL。结果:木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸与田蓟苷分离效果良好,分别在10.6～106.0 μg/mL(r=0.998 7)、11.5～115.2 μg/mL(r=0.999 4)、8.1～81.2 μg/mL(r=0.999 6)呈良好的线性关系;平均回收率为97.83 %、99.07 %、98.63 %;RSD值为1.03 %、1.44 %以及0.69 %。结论:该方法重复性好,结果准确可靠,为香青兰提取物中木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和田蓟苷成分的含量测定提供科学依据。
Objective: To establish a RP-HPLC method for determination of Luteolin-7-O-β-D-Glucuronide, rosmarinic acid and tilianin in ethyl acetate extract of Mongolia Dracocephalum moldavica L. Methods: The test was conducted on chromatographic column of Inertsil ODS-SP (4. 6 × 150nm, 5 um), and the mobile phase was acetonitrile (A)-0.1% formic acid (B) for gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm and the column temperature was 35 ℃, the injection volumn 10μL. Results: Luteolin-7-O-β-D-Glucuronide, rosmarinic acid and tilianin displayed good linear relationships within the ranges of 10.6~106.0 μg/mL (r=0.998 7), 11.5~115.2 μg/mL (r=0.999 4), and 8.1~81.2 μg/mL(r=0.999 6), and the average recoveries were 97.83 %, 99.07 %, and 98.63 %, with the RSDs of 1.03 %, 1.44 %, and 0.69 % respectively. Conclusion: The method is simple, accurate and reliability, and it can be used to provide scientific evidences for determination of Luteolin-7-O-β-D-Glucuronide, rosmarinus acid and tilianin in Mongolia D. moldavica Extracts.
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