目的: 探讨人参皂苷Rb1(GsRb1)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)模型小鼠的神经保护作用及量效关系。方法: 将50只C57BL/6小鼠随机分为5组,分别为对照组、MPTP组、GsRb1处理组(低剂量组、中剂量组和高剂量组),每组10只。模型组和处理组使用MPTP(30 mg/kg)连续腹膜腔注射5 d制备PD小鼠模型。处理组每天分别以10 mg/kg、20 mg/kg和40 mg/kg的剂量腹膜腔注射GsRb1;对照组给予等容量生理盐水。给药结束后第2 d,采用转棒实验、爬杆实验、悬挂实验等行为学方法检测小鼠的行为学变化。结果: 与对照组相比,MPTP组小鼠掉落潜伏期显著降低(P＜0.01),爬杆时间显著升高(P＜0.01),悬挂实验评分显著降低(P＜0.01)。与MPTP组比较,中、高剂量组掉落潜伏期明显升高(P＜0.05);低、中剂量组爬杆时间明显降低(P＜0.05);中、高剂量组悬挂实验评分明显升高(P＜0.05)。结论: GsRb1对MPTP诱导的PD模型小鼠具有神经保护作用,每天给予20 mg/kg效果较好。
Objective: To investigate the neuroprotective effect of ginsenoside Rb1 (GsRb1) on Parkinson's disease (PD) model mice induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) and its dose-effect relationship. Methods: Fifty C57BL/6 mice were randomly divided into five groups: control group, MPTP group, GsRb1 treatment group (low-dose group, middle dose group and high-dose group), with 10 mice in each group. The model group and the treatment group were injected MPTP (30 mg/kg) into the peritoneal cavity continuously for 5 days to prepare the PD mouse model. The treatment group received intraperitoneal injection of GsRb1 at doses of 10 mg/kg, 20 mg/kg and 40mg/kg respectively every day. The control group was given the same amount of normal saline. On the second day after administration, the behavioral changes of the mice were detected by using Rotarod test, Pole-climbing test and Grasping test. Results: Compared with the control group, the latent period of MPTP group mice was significantly reduced (P＜0.01), the climbing time was significantly increased (P＜0.01), and the Graspiing test score was significantly reduced (P＜0.01). Compared with the MPTP group, the latent period in the middle and high dose groups increased significantly (P＜0.05), the climbing time in low and middle dose groups decreased significantly (P＜0.05) and the scores of Graspiing test in middle and high dose groups were significantly higher (P＜0.05). Conclusion: GsRb1 has neuroprotective effect on MPT-induced PD model mice, and 20 mg/kg/d has a better effect.
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