目的: 通过化学诱导手段建立MC3T3-E1细胞成骨模型,探讨局部黏着斑激酶(FAK)基因表达与成骨细胞形成的关系,了解FAK对成骨分化的影响。方法: 观察MC3T3-E1细胞成骨诱导前后细胞形态,茜素红化学染色检测矿化情况,实时荧光定量PCR检测成骨标志物Runx2、ALP的表达,同时分析FAK在成骨过程中的表达情况。结果: MC3T3-E1细胞成骨诱导3 d后细胞呈长梭形或多角形;第21、28 d时,茜素红染色可见矿化结节;成骨标志物Runx2、ALP的表达呈持续升高的趋势,FAK的表达总体呈升高趋势,在第7 d时略有下降。结论: FAK的表达与成骨相关基因的表达趋势基本一致,提示FAK可能在MC3T3-E1细胞成骨过程中发挥一定作用。
Objective: To establish an osteogenic model of MC3T3-E1 cells by chemical induction, to explore the relationship between FAK gene expression and osteoblast formation, and to understand the effect of FAK on osteogenic differentiation. Methods: The cell morphology of MC3T3-E1 cells after osteogenesis induction was observed, mineralization was detected by alizarin red chemical staining, and the expression of osteogenic markers Runx2 and ALP was detected by real-time fluorescence quantitative PCR, while the expression of FAK in the osteogenesis process was analyzed. Results: MC3T3-E1 cells showed long shuttle or polygonal shape after 3 d of osteogenesis induction; mineralized nodules were visible by alizarin red staining at 21 d and 28 d. The expression of osteogenic markers Runx2 and ALP showed a continuous increase, and the expression of FAK showed an overall increasing trend, with a slight decrease at 7 d. Conclusion: The expression of FAK was basically consistent with the expression trend of osteogenesis-related genes, suggesting that FAK may play a role in the osteogenesis process of MC3T3-E1 cells.
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