目的: 研究闽产风柜斗草黄酮提取物的体外抗氧化作用并对风柜斗草黄酮进行定量分析。方法: 采用高效液相色谱法测定提取物中总黄酮醇苷的含量,并通过体外实验测定风柜斗草黄酮的体外抗氧化作用和抑制猪油过氧化反应。结果: 提取物中总黄酮醇苷的平均含量为3.359 mg/g。风柜斗草黄酮体外抗氧化的实验中发现,0.55 mg/mL风柜斗草黄酮对超氧阴离子自由基的清除率可达70.03 %;当风柜斗草黄酮的浓度在0.35～0.55 mg/mL时,对羟自由基清除的效果好,达到80 %以上。在抑制油脂过氧化反应中,风柜斗草黄酮粗粉能抑制过氧化值(POV)的升高。结论: 风柜斗草黄酮具有较好的体外抗氧化活性,是一种有效的天然自由基吸收剂。

Objective: To explore the antioxidation of lavonoids extracted from Sarcopyramis nepalensis Wall in South Fujian Province and make the quantitative analysis of lavonoids.Methods: HPLC was adopted to determine the total content of flavonol glycosides in the extract, and vitro experiments were carried out to determine the antioxidant effect of lavonoids extracted and the peroxidation of lard.Results: The average content of total flavonol glycosides in the extracts was 3.359mg/g. The scavenging rate of superoxide anion radical in 0.55 mg/mL flavonoids compounds was up to 70.03 %. When the concentration of flavonoids was 0.35～0.55 mg/mL, the scavenging effect of hydroxyl radical was good, reaching more than 80 %. It can effectively inhibit the increase of POV value in the inhibition of peroxidation lard.Conclusion: The lavonoids extracted has better anti-oxidative activity in vitro. It is an effective natural free radical absorber.