目的: 探讨淫羊藿苷(icariin, ICA)对成牙骨质细胞(OCCM-30)体外增殖及成骨分化能力的影响。方法: 通过体外培养OCCM-30细胞,加入不同浓度(10、20、40 μmol/L) ICA, 倒置显微镜下观察细胞形态。设置10 μmol/L ICA实验组与空白对照组处理,MTT法连续7 d检测OCCM-30细胞增殖率。通过Real-time PCR研究10 μmol/L ICA作用于OCCM-30细胞后(1、4、7 d)成骨相关因子OCN和Runx-2的表达。结果: 与对照组相比,加入ICA后OCCM-30细胞形态无显著变化,MTT结果显示10 μmol/L ICA明显促进OCCM-30细胞增殖(P＜0.05)。Real-time PCR结果显示实验组中成骨相关因子OCN和Runx-2的表达显著增高,与对照组相比较差异具有统计学意义(P＜0.05)。结论: 适宜浓度的ICA可有效促进OCCM-30的体外增殖及向成骨分化。
Objective: To investigate the effects of Icariin (ICA) on the proliferation and osteogenic differentiation of cementoblasts (OCCM-30) in vitro. Methods: OCCM-30 cells were cultured in vitro and added with different concentrations (10, 20, 40 μmol/L) of ICA and observed the cell morphology under inverted microscope. OCCM-30 cells was cultured in vitro and treated with blank control group and 10 μmol/L ICA group. The proliferation rate of OCCM-30 cells was detected by MTT method for 7 days. Real-time PCR was used to study the expression of osteogenic-related factors OCN and Runx-2 after 10 μmol/LICA was applied to OCCM-30 cells (1 d, 4 d, 7 d). Result: The results showed that there was no significant change in the morphology of OCCM-30 cells after adding ICA. MTT results showed that 10 μmol/L ICA significantly promoted the proliferation of OCCM-30 cells (P＜0.05). Real-time PCR results showed that the expression of osteogenic related factors OCN and Runx-2 in the experimental group was significantly higher than that in the control group (P＜0.05). Conclusion: Appropriate concentration of ICA can effectively promote the proliferation and osteogenic differentiation of OCCM-30 cells in vitro.
[1] Kumar S. Evidence-based update on diagnosis and management of gingivitis and periodontitis[J]. Dent Clin North Am, 2019, 63(1): 69-81.
[2] Kumar S, Madurantakam P. Limited evidence shows short-term benefit of probiotics when used as an adjunct to scaling and root planing in the treatment of chronic periodontitis[J]. Evid Based Dent, 2017, 18(4): 109-110.
[3] 朱亚桥, 陈丽娟, 刘一. 广泛型侵袭性牙周炎的牙周基础治疗长期疗效观察[J]. 临床口腔医学杂志, 2019, 35(7): 418-421.
[4] 张锦明, 田滢舟, 赵玲, 等. 淫羊藿苷促进骨髓间充质干细胞成骨分化缓解小鼠骨质疏松的机制[J]. 中国组织工程研究, 2022, 26(19): 2991-2996.
[5] 马小妮, 葛宝丰, 陈克明, 等. 淫羊藿苷通过OPG/RANKL信号途径调节骨吸收的机理研究[J]. 中国骨质疏松杂志, 2013, 19(1): 1-5.
[6] Xu HY, Zhou SR, Qu RY, et al. Icariin prevents oestrogen deficiency-induced alveolar bone loss through promoting osteogenesis via STAT3[J]. Cell Prolif, 2020, 53(2): e12743.
[7] Sun S, Liu L, Tian XJ, et al. Icariin attenuates high glucose-induced apoptosis, oxidative stress, and inflammation in human umbilical venous endothelial cells[J]. Planta Med, 2019, 85(6): 473-482.
[8] 姜涛, 凌翠敏, 陈庆真, 等. 淫羊藿苷通过提高自噬促进成骨细胞分化防治骨质疏松[J]. 中国组织工程研究, 2021, 25(17): 2643-2649.
[9] Zhang J, Fu J, Pan YL, et al. Silencing of miR-1247 by DNA methylation promoted non-small-cell lung cancer cell invasion and migration by effects of STMN1[J]. Onco Targets Ther, 2016, 9: 7297-7307.
[10] Zou L, Kidwai FK, Kopher RA, et al. Use of RUNX2 expression to identify osteogenic progenitor cells derived from human embryonic stem cells[J]. Stem Cell Reports, 2015, 4(2): 190-198.
[11] Zur Bruegge J, Backes C, Gölz G, et al. microRNA response of primary human macrophages to Arcobacter butzleri infection[J]. Eur J Microbiol Immunol (Bp), 2016, 6(2): 99-108.
[12] 涂艳, 熊莉娜, 柳湘洁, 等. 淫羊藿苷对成骨细胞成骨分化的影响及Wnt/-catenin信号系统的关系研究[J]. 中国中医急症, 2017, 26(3): 448-450, 466.
[13] 龚一昕. 淫羊藿次苷Ⅱ通过p38MAPK信号通路调控成骨细胞护骨素表达和成骨分化研究[D]. 合肥: 安徽医科大学, 2016.
[14] 吴曦, 彭锐. 不同浓度淫羊藿苷对人骨髓间充质干细胞成骨分化的影响[J]. 中国组织工程研究, 2018, 22(5): 669-674.
