组织蛋白酶B联合TRAIL诱导胃癌BGC-823细胞凋亡的作用研究*

查道德; 涂龙霞; 吴文涌

doi:10.16833/j.cnki.jbmc.2023.01.002

包头医学院学报 >

2023 , Vol. 39 >Issue 1: 4 - 7

DOI: https://doi.org/10.16833/j.cnki.jbmc.2023.01.002

基础医学论著

组织蛋白酶B联合TRAIL诱导胃癌BGC-823细胞凋亡的作用研究^*

查道德 ,
涂龙霞 ,
吴文涌

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1.淮北职业技术学院医学系, 安徽淮北 235000;
2.安徽省第二人民医院普外科

收稿日期: 2022-05-31

网络出版日期: 2023-03-07

基金资助

*安徽省重点研究与开发计划项目人口健康专项(202104j07020005);安徽省高校自然科学研究重点课题项目(KJ2018A0712);合肥市关键共性技术研发和重大科技成果工程化项目(2021YL001)

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Role of cathepsin B combining with TRAIL on inducing apoptosis in gastric cancer BGC-823 cells

ZHA Daode ,
TU Longxia ,
WU Wenyong

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1. Department of Medicine, Huaibei Vocational and Technical College, Huaibei 235000, China;
2. Department of General Surgery, Anhui Second People's Hospital

Received date: 2022-05-31

Online published: 2023-03-07

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摘要

目的: 观察组织蛋白酶B(CTSB)联合TRAIL诱导胃癌BGC-823细胞凋亡的作用。方法: BGC-823细胞传代培养。采用MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡率以及免疫印迹法(Western blot)检测Cathepsin B蛋白表达。结果: TRAIL对BGC-823细胞增殖有抑制作用,对细胞凋亡有促进作用(P＜0.05); Cathepsin B抑制剂浓度越高,细胞凋亡率越低(P＜0.05);Western blot结果表明,Cathepsin B抑制剂浓度越高,Cathepsin B表达越少(P＜0.05)。结论: Cathepsin B和TRAIL联合使用有协同诱导胃腺癌BGC-823细胞凋亡作用。

关键词： 组织蛋白酶B; 肿瘤坏死因子; 凋亡配体; BGC-823细胞; 细胞凋亡

本文引用格式

查道德 , 涂龙霞 , 吴文涌 . 组织蛋白酶B联合TRAIL诱导胃癌BGC-823细胞凋亡的作用研究^*[J]. 包头医学院学报, 2023 , 39(1) : 4 -7 . DOI: 10.16833/j.cnki.jbmc.2023.01.002

Abstract

Objective: To observe the apoptosis of gastric cancer BGC-823 cells induced by cathepsin B (CTSB) combined with TRAIL. Methods: BGC-823 cells were routinely subcultured. The proliferation of BGC-823 cells was detected with MTT Cell Proliferation Assay Kit. The apoptosis rate was detected by flow cytometry (FCM) and the expression level of cathepsin B protein was detected with Western blot. Results: TRAIL could inhibit the proliferation of BGC-823 cells and promote apoptosis(P＜0.05). The higher concentration of cathepsin B inhibitor could lead to the lower apoptosis rate(P＜0.05). Western blot results showed that the higher concentration of cathepsin B inhibitor would cause the lower expression of cathepsin B(P＜0.05). Conclusion: Cathepsin B combining with TRAIL could synergistically induce apoptosis of gastric cancer BGC-823 cells.

Key words： Cathepsin B; TRAIL; BGC-823 cell; Apoptosis

参考文献

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