目的:研究急性心肌梗死(acute myocardial infarction,AMI)患者血浆miRNA-208a-3p、miRNA-499a-5p、miRNA-221-3p三种miRNAs表达水平和与肌钙蛋白(cTnI)、心肌脂肪酸结合蛋白(H-FABP)的相关性及其对AMI早期诊断的潜在价值。方法:连续性收集52例AMI患者为AMI组,非心源性胸痛患者24例为对照组。采用高通量测序检测两组患者血浆中三种miRNAs表达水平,Pearson分析该三种miRNAs表达水平与cTnI、H-FABP的相关性;受试者工作特征曲线(ROC)分析三种miRNAs单独及联合诊断AMI的曲线下面积(AUC)。结果:与对照组相比,AMI组患者血浆中这三种miRNAs表达水平明显升高(P＜0.05),分别与cTnI、H-FABP呈正相关(P＜0.05);三者单独诊断AMI的AUC分别为0.794、0.772、0.806,联合诊断AMI的AUC为0.862。结论:血浆miRNA-208a-3p、miRNA-499a-5p、miRNA-221-3p可作为早期诊断AMI的潜在生物标志物,且联合指标的潜在诊断价值更高。
Objective: To investigate the expression levels of plasma miRNA-208a-3p, miRNA-499a-5p and miRNA-221-3p and their correlation with cardiac troponin (cTnⅠ) and heart fatty acid-binding protein (H-FABP) in patients acute myocardial infarction (AMI), and to explore their potential value for early diagnosis of AMI. Methods: A total of 52 patients with AMI were continuously collected as AMI group, and 24 patients with non-cardiac chest pain were collected as control group.High-throughput sequencing was used to detect the expression levels of three miRNAs in plasma of the two groups.Pearson analysis was used to analyze the correlation between the expression levels of three miRNAs and cTnⅠ and H-FABP.The receiver operating characteristic curve (ROC) was used to analyze the area under the curve (AUC) of the three miRNAs in the diagnosis of AMI alone and in combination. Results: Compared with the control group, the expression levels of these three miRNAs in the plasma of AMI group were significantly increased (P＜0.05), which were positively correlated with cTnⅠ and H-FABP respectively (P＜0.05), and the AUC of single diagnosis of AMI was 0.794, 0.772 and 0.806, respectively; the AUC of combined diagnosis of AMI was 0.862. Conclusion: Plasma miRNA-208a-3p, miRNA-499a-5p and miRNA-221-3p can be used as potential biomarkers for early diagnosis of AMI, and the potential diagnostic value of combined indicators is higher.
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