体外存储过程中红细胞衰亡的研究

doi:10.16833/j.cnki.jbmc.2022.09.001

包头医学院学报 ›› 2022, Vol. 38 ›› Issue (9): 1-4.doi: 10.16833/j.cnki.jbmc.2022.09.001

• 基础医学论著 • 下一篇

体外存储过程中红细胞衰亡的研究

杨丽丽¹, 丁慧荣², 李姝³, 李旭妍³, 孙明玥³, 苏燕³

1.鄂尔多斯市蒙医医院,内蒙古鄂尔多斯 017000;
2.北京大学肿瘤医院中心实验室;
3.包头医学院生物化学与分子生物学教研室

收稿日期:2021-06-21 出版日期:2022-09-25 发布日期:2022-09-09
通讯作者: 苏燕

Study on eryptosis of red blood cells during storage in vitro

YANG Lili¹, DING HuiRong², LI Shu³, LI XuYan³, SUN MingYue³, SU Yan³

1. Ordos Mongolia medicine Hospital, Ordos 017000, China;
2. Department of Biochemistry and Molecular Biology,Peking University Cancer Hospital & Institute;
3. Departmengt of Biochemistry and Molecular Biology, Baotou Medical College

Received:2021-06-21 Online:2022-09-25 Published:2022-09-09

摘要/Abstract

摘要： 目的:研究体外存储对红细胞衰亡(eryptosis)的影响。方法:募集6名健康成年志愿献血者,分别采集400 mL抗凝全血,滤白后离心去除血浆,将红细胞(red blood cells,RBC)重悬于100 mL MAP红细胞保护液中,4 ℃保存。于储存期0、7、14、21、28和35 d 分别抽取血样6 mL,利用流式细胞仪、全自动血细胞计数仪、血气分析仪等分别检测RBC膜磷脂酰丝氨酸(phosphatidylserine,PS)含量、红细胞平均体积(mean corpuscular volume,MCV)、细胞外离子浓度、细胞外葡萄糖(glucose,Glu)、乳酸(lactic acid,Lac)、总胆红素(total bilirubin,TBil)浓度及高铁血红蛋白分数(fraction of methemoglobin,FMetHb)。结果:随着存储时间延长,PS含量降低(r=-0.8356),MCV、细胞外Cl^-浓度没有变化,细胞外TBil浓度(r=0.8035)、Lac浓度(r=0.9377)及FMetHb(r=0.9706)随存储时间延长不断增加,细胞内Na⁺浓度(r=-0.9274)和Glu浓度(r=-0.9860)随存储时间延长降低。结论:体外存储并不引起RBC衰亡增加。

关键词: 红细胞, 体外存储, 衰亡

Abstract: Objective: To study the effect of storage in vitro on Eryptosis. Methods: Recruit 6 healthy adult volunteer blood donors, collect 400 mL of anticoagulated whole blood, filter and remove the plasma by centrifugation, resuspend red blood cells (RBC) in 100 mL MAP red blood cell protection solution, and store at 4 ℃ . 6 mL of blood samples were drawn at the 0, 7, 14, 21, 28, and 35th day of the storage period respectively. The RBC membrane Phosphatidylserine (PS) content was measured by flow cytometry, automatic blood cell counter, blood gas analyzer, etc, mean corpuscular volume (MCV), extracellular ion concentration, extracellular glucose (Glucose, Glu), lactic acid (Lactic acid, Lac), total bilirubin (Total bilirubin, TBil) concentration and methemoglobin fraction (Fraction of Methemoglobin, FMetHb). Results: With the prolonged storage time, PS content decreased (r=-0.8356), MCV, extracellular Cl^- concentration did not change, extracellular TBi concentration (r=0.8035) ,Lac concentration (r=0.9377) and FMetHb (r=0.9706) .The concentration of Na⁺ (r=-0.9274) and Glu (r=-0.9860) in the cells decreased with the extension of storage time. Conclusion: In vitro storage does not cause increased RBC death.

Key words: red blood cells, storage, eryptosis

杨丽丽, 丁慧荣, 李姝, 李旭妍, 孙明玥, 苏燕. 体外存储过程中红细胞衰亡的研究[J]. 包头医学院学报, 2022, 38(9): 1-4.

YANG Lili, DING HuiRong, LI Shu, LI XuYan, SUN MingYue, SU Yan. Study on eryptosis of red blood cells during storage in vitro[J]. Journal of Baotou Medical College, 2022, 38(9): 1-4.

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体外存储过程中红细胞衰亡的研究

Study on eryptosis of red blood cells during storage in vitro

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