Objective: To explore the effect of over-expressing WNT11 on osteogenesis of osteoblasts under LPS-incurred inflammatory condition in vitro. Methods: The objective gene sequences targeting for WNT11 were transduced into osteoblasts by lentivirus, then the WNT11 protein level was tested by Western blot assay from the functional perspective. The cells after transduction were evaluated the cell proliferation activity by the CCK-8 method. The experimental cells were enrolled into 3 groups in vitro: non-induced group with LPS, general induced group with LPS and over-expressing WNT11 induced group with LPS. All cells were induced in vitro by alizarin red staining, mineral nodules and alkaline phosphatase (ALP) semi-quantitative analysis, and qRT-PCR was used to analyze the expression levels of osteogenic specific genes ALP, runt-related transcription factor 2 (Runx2), osteocalcin (OC) and collagen I mRNA after induction, and the differences in osteogenic ability were comprehensively evaluated. Results: After transduction using lentivirus, the results of Western blot assay showed the WNT11 protein level was significantly higher than that in control group (P＜0.001). The cell proliferation activity demonstrated no significant differences after transduction, when compared with non-transduction at any time point (P＞0.05). There was no significant apoptosis of osteoblasts cultured in LPS (100 ng/mL) concentration. After 1 week and 2 weeks of induction, mineralized nodules were observed in the induction group and gradually increased with time, while no obvious mineralized nodules were observed in the control group.The quantitatively analysis results of mineralization nodules and ALP activity were significant higher in WNT11 over-expressing group than in general induced group (P＜0.000 1). Moreover, the results of osteogenic relate genes demonstrated similar trends. Conclusion: Over-expressing WNT11 may significantly enhances the osteogenic ability of osteoblasts under LPS-incurred inflammatory condition.
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