Objective: To explore the role and molecular mechanism of down-regulation of thioredoxin system in cigarette smoke extract (CSE) -induced human bronchial epithelial cell (BEAS-2B) injury model, so as to provide experimental basis for finding potential prevention and treatment targets of smoking-related diseases. Methods: CCK-8 method was used to detect the effect of different concentrations of CSE on the viability of BEAS-2B cells, and the treatment concentration of CSE was screened to establish a cell injury model. Lentivirus LentiCRISPRv 2 plasmid was constructed to knock down thioredoxin-1 (Trx-1) in BEAS-2B cells. The cells were divided into control group, 10% CSE treatment group, knockdown group and knockdown + 10% CSE treatment group. CCK-8 method was used to detect cell activity. RT-qPCR method was used to detect the expression levels of inflammatory factors, Trx-1 and thioredoxin reductase (TrxR) mRNA. Western blot method was used to detect the expression levels of Trx-1 and TrxR proteins. Flow cytometry was used to detect the level of reactive oxygen species (ROS). Results: After CSE intervention in BEAS-2B cells for 24 hours, the cell viability of BEAS-2B cells gradually decreased with increasing CSE concentration (P＜0.05). The protein expression levels of Trx1 and TrxR were downregulated (P＜0.001), and their mRNA expression levels were also downregulated (P＜0.05). The mRNA expression levels of the inflammatory cytokines IL-6, IL-8, and IL-1β in the cell model were upregulated (P＜0.001), and the ROS level was increased (P＜0.000 1). Following Trx-1 knockdown, compared with the control group, cell viability after CSE exposure was more markedly decreased (P＜0.000 1), and the mRNA expression levels of the inflammatory cytokines were all increased (P＜0.000 1). Conclusion: CSE induces injury in BEAS-2B cells. Decreased Trx-1 levels in the cells exacerbate CSE-induced inflammatory responses and oxidative stress injury in BEAS-2B cells. These findings suggest that downregulation of the Trx system may mediate CSE-induced injury in BEAS-2B cells.
YANG Yixuan
,
YANG Sen
,
ZHANG Kun
,
SUN Jie
,
LIN Yi
,
TAO Weiwei
,
LIU Jia
. Role of down-regulation of thioredoxin system in CSE-induced BEAS-2B cell injury[J]. Journal of Baotou Medical College, 2026
, 42(4)
: 41
-46
.
DOI: 10.16833/j.cnki.jbmc.2026.04.007
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