Objective: To explore the mechanism by which pressure affects osteogenesis of dura mater cells through the FAK-PI3K-AKT signaling pathway. Methods: After osteogenic induction of dura mater cells, the morphological changes of the cells were observed under an inverted microscope. Alizarin red staining and alkaline phosphatase staining were used to evaluate the formation of calcium nodules. The mRNA expression levels of focal adhesion kinase (FAK), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (AKT) under pressure intervention were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The expression levels of p-AKT, PI3K, and p-FAK proteins in the FAK-PI3K-AKT signaling pathway under pressure intervention were detected by Western blot. Results: After osteogenic induction, the morphological characteristics of dura mater cells changed significantly, gradually transforming from fusiform to short fusiform or polygonal, with elongated and flattened cell bodies and significantly increased cell and nuclear volumes. The results of alizarin red staining and alkaline phosphatase staining showed that the cell staining was significantly deepened after osteogenic induction. Under pressure stimulation, the mRNA levels of AKT, PI3K, and FAK in dura mater cells and the protein expression levels of p-AKT, PI3K, and p-FAK were all increased. The expression levels of osteogenesis-related proteins osteocalcin (OCN) and alkaline phosphatase (ALP) were significantly upregulated. Conclusion: After osteogenic induction of dura mater cells, the number of osteogenic nodules increased over time. The mechanism may be achieved by activating the FAK-PI3K-AKT signaling pathway, thereby increasing the expression of osteogenesis-related proteins.
SU Dula
,
ZHAO Lijun
,
ZHANG Chunyang
,
ZHU Meilin
,
BAO Lijuan
. The molecular mechanism of pressure affecting the osteogenesis of dural cells through the FAK-PI3K-AKT signaling pathway[J]. Journal of Baotou Medical College, 2026
, 42(3)
: 38
-44
.
DOI: 10.16833/j.cnki.jbmc.2026.03.008
[1] 寇正雄, 张海燕, 侯晓峰, 等.硬脑膜对颅骨生长影响的研究进展[J].中国医刊, 2021, 56(10): 1059-1062.
[2] 张司玺, 张晓璐, 王雷, 等.FAK-PI3K/AKT信号通路在新生大鼠颅盖骨生长发育过程中作用机制的研究[J].安徽医科大学学报, 2023, 58(1): 109-115.
[3] 安冉, 邵国, 张春阳.大鼠硬脑膜对颅骨成骨增强的影响[J].中国组织工程研究, 2024, 28(22): 3478-3483.
[4] 董新航.Crouzon综合征疾病模型中硬脑膜细胞介导FGFR2对成骨细胞学水平的影响及调控Hippo/YAP与PI3K-AKT信号通路的实验研究[D].北京: 北京协和医学院, 2022.
[5] Ghezzi L, Cantoni C, Cignarella F, et al.T cells producing GM-CSF and IL-13 are enriched in the cerebrospinal fluid of relapsing MS patients[J].Multiple Sclerosis, 2020, 26(10): 1172-1186.
[6] Hsu S J, Zhang C, Jeong J, et al.Enhanced meningeal lymphatic drainage ameliorates neuroinflammation and hepatic encephalopathy in cirrhotic rats[J].Gastroenterology, 2021, 160(4): 1315-1329.e13.
[7] Wu M, Xu T, Zhou Y, et al.Pressure and inflammatory stimulation induced increase of cadherin-11 is mediated by PI3K/Akt pathway in synovial fibroblasts from temporomandibular joint[J].Osteoarthritis and Cartilage, 2013, 21(10): 1605-1612.
[8] Petrie C, Tholpady S, Ogle R, et al.Proliferative capacity and osteogenic potential of novel dura mater stem cells on poly-lactic-co-glycolic acid[J].Journal of Biomedical Materials Research Part A, 2008, 85(1): 61-71.
[9] Peptan I A, Hong L, Evans C A.Multiple differentiation potentials of neonatal dura mater-derived cells[J].Neurosurgery, 2007, 60(2): 346-352.
[10] Moazen M, Alazmani A, Rafferty K, et al.Intracranial pressure changes during mouse development[J].Journal of Biomechanics, 2016, 49(1): 123-126.
[11] Matsumoto T.Osteoporosis andMechano-biosciences[J].Clinical Calcium, 2016, 26(12): 1717-1727.
[12] Lunde KB, Hellem S, Bogstad J, et al.Pressure during compaction of morsellised bone gives an increase in stiffness: an in vitro study[J].Journal of Biomechanics, 2008, 41(1): 231-234.
[13] Gusmão CVB, Batista NA, Lemes VT, et al.Effect of low-intensity pulsed ultrasound stimulation, extracorporeal shockwaves and radial pressure waves on Akt, BMP-2, ERK-2, FAK and TGF-β1 during bone healing in rat tibial defects[J].Ultrasound in Medicine and Biology, 2019, 45(8): 2140-2161.
[14] Wu HJ, Zhang ZQ, Yu B, et al.Pressure activates Src-dependent FAK-Akt and ERK1/2 signaling pathways in rat hepatic stellate cells[J].Cellular Physiology and Biochemistry, 2010, 26(3): 273-280.
[15] 米杜秋.不同压力高压氧治疗颅脑损伤后失语症患者的临床效果[J].医疗装备, 2017, 30(15): 136-137.
[16] 易超亮, 周广.不同压力下高压氧治疗脑外伤临床研究[J].深圳中西医结合杂志, 2021, 31(23): 37-40.
[17] Oyaizu T, Enomoto M, Yamamoto N, et al.Hyperbaric oxygen reduces inflammation, oxygenates injured muscle, and regenerates skeletal muscle via macrophage and satellite cell activation[J].Scientific Reports, 2018, 8(1): 1288.
[18] Li L, Xia Y, Wang Z, et al.Suppression of the PI3K-Akt pathway is involved in thedecreased adhesion and migration of bone marrow-derived mesenchymal stem cells from non-obese diabetic mice[J].Cell Biology International, 2011, 35(9): 961-966.
