Objective: To investigate the damage of neodymium oxide (Nd2O3) exposure on mitochondria of astrocytes (AST) and the role of tunneling nanotubes (TNTs) in it. Methods: AST was extracted from SD rats within 3 days of birth, and the specific expression of GFAP was identified by immunofluorescence. CCK8 kit was used to detect the cell survival rate after neodymium oxide exposure. DCFH-DA probe was used to detect intracellular reactive oxygen species; MitoSOX Red was used to detect the reactive oxygen species in mitochondria. Mitochondrial membrane potential was detected by Rhodamine 123. The live cell staining markers F-actin, β-tubulin and mitochondria were used to observe the structure, quantity and mitochondrial transmission of TNTs. Results: The extracted astrocytes specifically expressed GFAP; the optimal exposure dose and time were determined by cell viability testing to be 10 μg/mL Nd2O3 and 12 hours. After Nd2O3 exposure, the levels of intracellular reactive oxygen species and mitochondrial reactive oxygen species were significantly increased, and the mitochondrial membrane potential was decreased; the structure of TNTs in AST-AST was dominated by F-actin; and after Nd2O3 exposure, the number of TNTs in ASTs was increased, while TNTs mediated mitochondrial transmission in ASTs after Nd2O3 exposure. Conclusion: The increased level of oxidative stress in AST caused by Nd2O3 has been demonstrated to trigger mitochondrial damage and to mediate intercellular mitochondrial transmission via TNTs.
CAO Jing
,
GAO Xiaocheng
,
GAO Lei
,
LIANG Qingqing
,
JIA Hailian
,
HUO Zhi
,
ZHAO Xin
,
DENG Yang
. Study on the role of tunneling nanotubes in mitochondrial damage in astrocytes caused by neodymium oxide[J]. Journal of Baotou Medical College, 2026
, 42(3)
: 1
-6
.
DOI: 10.16833/j.cnki.jbmc.2026.03.001
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