Objective: To optimize the bacterial growth curve determination method and systematically evaluate the effects of detection devices (spectrophotometer vs. microplate reader), culture methods (batch vs. aliquoted tube), and detection timing (real-time vs. endpoint) on growth curve analysis. Methods: Using Escherichia coli and Staphylococcus aureus as model strains, a three-factor orthogonal experimental design (8 combinations) was implemented. Optical density values were measured via spectrophotometer (OD600) and microplate reader (OD630) to plot growth curves. The growth curve was drawn and the correlation between devices, time sequence equivalence and culture stability were analyzed. Results: Growth curves under different method combinations showed strong positive correlations (P＜0.01). No significant differences were observed between real-time and endpoint refrigeration detection in OD values (P＞0.05). The optimized tube culture-endpoint-microplate reader (OD630) detection method significantly improved the smoothness of the curve by pre-mixed inoculation standardization and single-tube continuous sampling. Conclusion: The integrated protocol combining tube pre-mixed culture, 4 ℃ refrigeration endpoint detection, and microplate reader-based high-throughput analysis (OD630) improves operational efficiency and data consistency, providing an optimization scheme for bacterial growth curve determination.
HUO Chenhui
,
PAN Xiangying
,
CAI Lin
,
YU Yangyang
,
ZHAO Jia
,
MAO Yixuan
,
ZHANG Meiying
. Modified methods for determining bacterial growth curves based on tube culture and microplate reader[J]. Journal of Baotou Medical College, 2026
, 42(1)
: 80
-84
.
DOI: 10.16833/j.cnki.jbmc.2026.01.014
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