Objective: To investigate the effect of liver kinase B1 ( LKB1 ) gene on the proliferation, migration and invasion of lung cancer cells and its mechanism. Methods: LKB1 overexpression lentivirus was transfected into A549 cells, and A549 blank group (without transfection of lentivirus), NC-A549 group (transfected with empty vector) and LKB1-A549 group (transfected with LKB1 gene) were established. Western blot and RT-qPCR were used to detect the expression level of LKB1 after transfection. CCK8 assay was used to detect the effect of LKB1 overexpression on the proliferation of A549 cells. Transwell assay was used to detect the effect of LKB1 overexpression on the migration and invasion of A549 cells. The effect of LKB1 overexpression on the expression of epithelial-mesenchymal transition markers was detected by RT-qPCR. The effects of LKB1 overexpression on the expression of vascular endothelial growth factor A and tropomyosin 1 were detected by Western blot. Results: Compared with the A549 blank group, the expression of LKB1 in the LKB1-A549 group was increased (P＜0.01), the cell proliferation, migration and invasion abilities were decreased (P＜0.01), the expression of epithelial-mesenchymal transition marker E-cadherin was increased (P＜0.01), and the expression of N-cadherin was decreased (P＜0.01). The expression of vascular endothelial growth factor A (VEGFA) and tropomodulin-1 (TMOD1) decreased (P＜0.01). Conclusion: LKB1 overexpression inhibits the proliferation, migration, invasion, epithelial-mesenchymal transition and angiogenesis of human lung cancer A549 cells, which may become a potential target for lung cancer treatment.
ZHAO Zongting
,
WANG Xinxin
,
JIA Jianxin
,
CHEN Xiaoyan
. Effect and mechanism of LKB1 on the proliferation, migration and invasion of lung cancer cells[J]. Journal of Baotou Medical College, 2026
, 42(1)
: 56
-61
.
DOI: 10.16833/j.cnki.jbmc.2026.01.010
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