Objective: To investigate the effect of isoliquiritigenin on the migration of gastric cancer cells and its possible mechanism. Methods: The cultured gastric cancer cells HGC-27 were divided into control group, 50 μmol/L ISL group and 75 μmol/L ISL group, and AGS cells were divided into control group, 100 μmol/L ISL group and 150 μmol/L ISL group. The number of cells migrated in each group was detected by scratch test and Transwell migration test. The mRNA expression levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in each group were determined via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results: As the concentration of isoliquiritigenin rose, there was a marked reduction in the migratory cell counts of HGC-27 and AGS cells (P＜0.05). RT-qPCR results showed that the relative expression levels of MMP-2 and MMP-9 mRNA in Isoliquiritigenin intervention groups were significantly lower than those in blank control group (P＜0.05). Conclusion: Isoliquiritigenin may inhibit the migration of HGC-27 and AGS by decreasing the MMP-2 and MMP-9 expression.
SHEN Yingxue
,
DONG Wenjie
,
GAO Fang
,
JIA Yanbin
. Effect of isoliquiritigenin on the migration of gastric cancer cells[J]. Journal of Baotou Medical College, 2026
, 42(1)
: 42
-46
.
DOI: 10.16833/j.cnki.jbmc.2026.01.008
[1] Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2024, 74(3):229-263.
[2] Peng F, Du Q, Peng C, et al. A review: the pharmacology of isoliquiritigenin[J].Phytother Res, 2015, 29(7): 969-977.
[3] 辛红, 孙鹏然, 宋鹏, 等.异甘草素对人肾透明细胞癌786-O细胞抗癌作用及分子机制[J].中国实验方剂学杂志, 2019, 25(12):83-89.
[4] Wang Z, Li W, Wang X, et al. Isoliquiritigenin induces HMOX1 and GPX4-mediated ferroptosis in gallbladder cancer cells[J]. Chin Med J (Engl), 2023, 136(18):2210-2220.
[5] Shuman Moss LA, Jensen-Taubman S, Stetler-Stevenson WG. Matrixmetalloproteinases: changing roles in tumor progression and metastasis[J]. Am J Pathol, 2012 , 181(6):1895-1899.
[6] Lu YT, Hsin CH, Kao SH, et al. Isoliquiritigenin diminishes invasiveness of human nasopharyngeal carcinoma cells associating with inhibition of MMP-2 expression and STAT3 signalling[J]. J Cell Mol Med, 2024, 28(15):e18586.
[7] Ganesan K, Xu C, Xie C, et al. Cryoprotective isoliquiritigenin-zein phosphatidylcholine nanoparticles inhibits breast cancer-bone metastasis by targeting JAK-STAT signaling pathways[J].Chem Biol Interact, 2024, 396: 111037.
[8] Lee CH, Tsai HY, Chen CL, et al. Isoliquiritigenin inhibits gastric cancer stemness, modulates tumor microenvironment, and suppresses tumor growth through Glucose-regulated protein 78 downregulation[J]. Biomedicines, 2022, 10(6):1350.
[9] Wu S, Wang J. Isoliquiritigenin regulates the circ_0002860/miR-431-5p/RAB9A axis to function as a tumor inhibitor in melanoma[J]. J Venom Anim Toxins Incl Trop Dis, 2023, 29:e20220019.
[10] 李杨. xCT调节肝癌细胞转移的作用机制研究[D].天津:天津医科大学, 2014.
[11] 赵静丽, 季峰.胃癌细胞转移相关促移动因子研究进展[J].国际消化病杂志, 2009, 29(3):178-180.
[12] Satelli A, Li S. Vimentin in cancer and its potential as a molecular target for cancer therapy[J]. Cell Mol Life Sci, 2011, 68(18):3033-3046.
[13] Na TY, Schecterson L, Mendonsa AM, et al. The functional activity of E-cadherin controls tumor cell metastasis at multiple steps[J]. Proc Natl Acad Sci U S A, 2020, 117(11):5931-5937.
[14] Kai F, Drain AP, Weaver VM. The extracellular matrix modulates the metastatic journey[J]. Dev Cell, 2019, 49(3):332-346.
[15] Ardizzone A, Bova V, Casili G, et al. Role of basic fibroblast growth factor in cancer: biological activity, targeted therapies, and prognostic value[J]. Cells, 2023, 12(7):1002.
[16] Lee CH, Tsai HY, Chen CL, et al. Isoliquiritigenin inhibits gastric cancer stemness, modulates tumor microenvironment, and suppresses tumor growth through Glucose-regulated protein 78 downregulation[J]. Biomedicines, 2022, 10(6):1350.
[17] Ganesan K, Xu C, Du B, et al. Isoliquiritigenin: A natural compound with a promising role in inhibiting breast cancer lung metastasis[J]. J Tradit Complement Med, 2024, 2225-4110.
