Objective: To explore the mechanism of concentrated growth factor (CGF) in the autologous repair of skull defect. Methods: Without damaging the dura mater, a circular defect with a diameter of 3 mm was made in the left parietal bone of 2-week-old rats. After removal of the bone flap, they were divided into the Control group and the CGF group. Subsequently, 5 μg of hydrogel and CGF gel were respectively added to the skull defect sites of the two groups, and the skin was sutured. The experiment lasted for a total of 4 weeks. Six rats from each group were sacrificed weekly to measure the remaining area of the defect and other physiological indexes. After 4 weeks, the remaining 16 rats in each group were sacrificed to obtain samplesfor determination of the expression levels of SMAD1, RUNX2, and OSX in the skull and dura mater at the defect site by Western-blot and Real-time qPCR. Results: At the end of the experiment, the defect area of the rats in the CGF group was significantly smaller than that in the Control group, with statistical significance (P＜0.001). The results of HE staining showed that the number of new bone trabeculae and inflammatory factors in the defect area of the CGF group was significantly different from that of the Control group. Western-blot and Real-time qPCR showed that the expression levels of SMAD1, RUNX2, and OSX in the skull and dura mater of the CGF group were significantly higher than those of the Control group, with statistical significance (P＜0.001). Conclusion: Under the effect of CGF, the speed of autologous repair of skull defect is significantly accelerated, and the mRNA and protein expression of SMAD1, RUNX2 and OSX in skull and dura mater is enhanced. The dura mater may play an important role in promoting the process of autologous repair of skull defects through the SMAD1 / RUNX2 / OSX pathway.
FENG Zilong
,
BAO Jianmin
,
SHAO Guo
,
ZHAO Zhijun
,
ZHANG Chunyang
,
SUN Zhigang
. Research on the mechanism of CGF affecting the autologous repair of skull defect[J]. Journal of Baotou Medical College, 2025
, 41(12)
: 33
-39
.
DOI: 10.16833/j.cnki.jbmc.2025.12.007
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