Objective: To explore the effect of miR-204-5p on the proliferation, migration and invasion of bladder cancer cells by specifically regulating the expression level of TCF12, and to further reveal its mechanism. Methods: The expression levels of miR-204-5p in bladder cancer cells and human urothelial cells, as well as bladder cancer tissues and adjacent tissues were detected by qRT-PCR. CCK-8, Transwell and flow cytometry were used to verify the effect of miR-204-5p on bladder tumor cells. The dual luciferase assay was used to verify the targeting relationship between miR-204-5 p and TCF12. Western-blot and qRT-PCR were used to detect the regulatory effect of miR-204-5p on the expression of TCF12. Results: The expression of miR-204-5p was significantly up-regulated in bladder cancer tissues and cell lines (P＜0.05). The overexpression of miR-204-5p was confirmed to promote the progression of bladder cancer in vitro (P＜0.05). Studies had confirmed that TCF12 was a potential target gene of miR-204-5p, and down-regulation of TCF12 by miR-204-5p promoted the proliferation, migration and invasion of bladder cancer cells. Overexpression of TCF12 could also reverse the effect of miR-204-5p overexpression on bladder cancer cells. Conclusion: miR-204-5p may promote the proliferation, migration and invasion of bladder cancer cells by targeting TCF12 and down-regulating its expression level. Targeting miR-204-5p / TCF12 may provide a potential therapeutic strategy for bladder cancer.
GAO Wuyue
,
ZOU Zhenhai
,
ZHANG Shuchao
,
WANG Zhongqi
,
LIU Jianmin
,
WANG Chao
,
GUO Yuanyuan
. The mechanism of miR-204-5p promoting the biological behavior of bladder cancer cells by down-regulating TCF12[J]. Journal of Baotou Medical College, 2025
, 41(11)
: 18
-23
.
DOI: 10.16833/j.cnki.jbmc.2025.11.004
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