Objective: To explore the effect and mechanism of cynomorium songaricum flavonoids (CSF) on senescence-accelerated mice prone 8 (SAMP8). Methods: Thirty 7-month-old male SAMP8 mice were selected and randomly divided into control group, cynomorium songaricum flavonoids group, and donepezil group. The SAMP8 mice in the cynomorium songaricum flavonoids group were given cynomorium songaricum flavonoids 50 mg/(kg·d) by gavage for 20 days, and the donepezil group was given donepezil 0.71 mg/(kg·d) by gavage for 20 days. After administration, the Morris water maze was used to evaluate the cognitive function, and Nissl staining was used to observe the morphology and count of hippocampal pyramidal cells. The protein expression levels of NADPH: quinone oxidoreductase-1 (NOQ1), nuclear factor e2 related factor 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) in hippocampus were determined by Western blotting. The activity of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) and the content of malondialdehyde(MDA) in serum were measured by ELISA. Results: Compared with the control group, the distance and escape latency of the mice in the cynomorium songaricum flavonoids were decreased, and the percentage of the number of crossing the platform and the time of the target quadrant were increased (P＜0.05). Compared with the control group, the expression of Nrf2, Keap1 and NOQ1 protein in the hippocampus of the mice in the cynomorium songaricum flavonoids group and donepezil group was increased (P＜0.05). Compared with the control group, the activity of SOD and GSH-Px in the serum of mice in the cynomorium songaricum flavonoids group was increased (P＜0.05), and the content of MDA was decreased (P＜0.05). Conclusion: Cynomorium songaricum flavonoids can improve cognitive dysfunction in SAMP8 mice, and its mechanism may be related to regulating the Nrf2 Keap1 pathway to inhibit oxidative stress.
ZHANG Jianping
,
ZHAO Jing
,
SHI Ershuan
,
FANG Gang
,
JIA Jianxin
,
SONG Wei
,
ZHOU Ruiqing
. Improvement effect of cynomorium songaricum flavonoids on cognitive dysfunction in SAMP8 mice and its possible mechanism[J]. Journal of Baotou Medical College, 2025
, 41(11)
: 13
-17
.
DOI: 10.16833/j.cnki.jbmc.2025.11.003
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